DNASU Plasmid Repository • 480.965.5697 | Email

AMACR (Homo sapiens) in pDONR221 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  AMACR
Gene Name:  alpha-methylacyl-CoA racemase
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH266489.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00081865

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 594nts         Open reading frame : 1 to 594
Reference Sequence Alignment

ATGGCACTGCAGGGCATCTCGGTCATGGAGCTGTCCGGCCTGGCCCCGGGCCCGTTCTGT
GCTATGGTCCTGGCTGACTTCGGGGCGCGTGTGGTACGCGTGGACCGGCCCGGCTCCCGC
TACGACGTGAGCCGCTTGGGCCGGGGCAAGCGCTCGCTAGTGCTGGACCTGAAGCAGCCG
CGGGGAGCCGCCGTGCTGCGGCGTCTGTGCAAGCGGTCGGATGTGCTGCTGGAGCCCTTC
CGCCGCGGTGTCATGGAGAAACTCCAGCTGGGCCCAGAGATTCTGCAGCGGGAAAATCCA
AGGCTTATTTATGCCAGGCTGAGTGGATTTGGCCAGTCAGGAAGCTTCTGCCGGTTAGCT
GGCCACGATATCAACTATTTGGCTTTGTCAGGTGGAAGGAACAGCATATTTAAGTTCTTT
TCTGTGGAAAACTCAGAAATCGAGTCTGTGGGAAGCACCTCGAGGACAGAACATGTTGGA
TGGTGGAGCACCTTTCTATACGACTTACAGGACAGCAGATGGGGAATTCATGGCTGTTGG
AGCAATAGAACCCCAGTTCTACGAGCTGCTGATCAAAGGACTTGGACTAAAGTC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 609
Start on reference sequence 13
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagctggcaCC

3' Linker Sequence:
GGCGacccagctttcttgtaca

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : AMACR
Symbol Nomenclature : AMACR
SYNONYM : AMACRD; CBAS4; RACE; RM
Designation : 2-methylacyl-CoA racemase
Full Nomenclature : alpha-methylacyl-CoA racemase
GENEID : 23600
GI : 117646775
GenBank Accession : AM392625
HGNC : 451
MIM : 604489
Vega : OTTHUMG00000090734
Target GenBank: BC009471

 

Reference Sequence Alignment

  

HsCD00081865        ATGGCACTGCAGGGCATCTCGGTCATGGAGCTGTCCGGCCTGGCCCCGGGCCCGTTCTGT
NM_203382.2         ATGGCACTGCAGGGCATCTCGGTCGTGGAGCTGTCCGGCCTGGCCCCGGGCCCGTTCTGT
NM_001167595.1      ATGGCACTGCAGGGCATCTCGGTCGTGGAGCTGTCCGGCCTGGCCCCGGGCCCGTTCTGT
NM_014324.5         ATGGCACTGCAGGGCATCTCGGTCGTGGAGCTGTCCGGCCTGGCCCCGGGCCCGTTCTGT
                    ************************.***********************************

HsCD00081865        GCTATGGTCCTGGCTGACTTCGGGGCGCGTGTGGTACGCGTGGACCGGCCCGGCTCCCGC
NM_203382.2         GCTATGGTCCTGGCTGACTTCGGGGCGCGTGTGGTACGCGTGGACCGGCCCGGCTCCCGC
NM_001167595.1      GCTATGGTCCTGGCTGACTTCGGGGCGCGTGTGGTACGCGTGGACCGGCCCGGCTCCCGC
NM_014324.5         GCTATGGTCCTGGCTGACTTCGGGGCGCGTGTGGTACGCGTGGACCGGCCCGGCTCCCGC
                    ************************************************************

HsCD00081865        TACGACGTGAGCCGCTTGGGCCGGGGCAAGCGCTCGCTAGTGCTGGACCTGAAGCAGCCG
NM_203382.2         TACGACGTGAGCCGCTTGGGCCGGGGCAAGCGCTCGCTAGTGCTGGACCTGAAGCAGCCG
NM_001167595.1      TACGACGTGAGCCGCTTGGGCCGGGGCAAGCGCTCGCTAGTGCTGGACCTGAAGCAGCCG
NM_014324.5         TACGACGTGAGCCGCTTGGGCCGGGGCAAGCGCTCGCTAGTGCTGGACCTGAAGCAGCCG
                    ************************************************************

HsCD00081865        CGGGGAGCCGCCGTGCTGCGGCGTCTGTGCAAGCGGTCGGATGTGCTGCTGGAGCCCTTC
NM_203382.2         CGGGGAGCCGCCGTGCTGCGGCGTCTGTGCAAGCGGTCGGATGTGCTGCTGGAGCCCTTC
NM_001167595.1      CGGGGAGCCGCCGTGCTGCGGCGTCTGTGCAAGCGGTCGGATGTGCTGCTGGAGCCCTTC
NM_014324.5         CGGGGAGCCGCCGTGCTGCGGCGTCTGTGCAAGCGGTCGGATGTGCTGCTGGAGCCCTTC
                    ************************************************************

HsCD00081865        CGCCGCGGTGTCATGGAGAAACTCCAGCTGGGCCCAGAGATTCTGCAGCGGGAAAATCCA
NM_203382.2         CGCCGCGGTGTCATGGAGAAACTCCAGCTGGGCCCAGAGATTCTGCAGCGGGAAAATCCA
NM_001167595.1      CGCCGCGGTGTCATGGAGAAACTCCAGCTGGGCCCAGAGATTCTGCAGCGGGAAAATCCA
NM_014324.5         CGCCGCGGTGTCATGGAGAAACTCCAGCTGGGCCCAGAGATTCTGCAGCGGGAAAATCCA
                    ************************************************************

HsCD00081865        AGGCTTATTTATGCCAGGCTGAGTGGATTTGGCCAGTCAGGAAGCTTCTGCCGGTTAGCT
NM_203382.2         AGGCTTATTTATGCCAGGCTGAGTGGATTTGGCCAGTCAGGAAGCTTCTGCCGGTTAGCT
NM_001167595.1      AGGCTTATTTATGCCAGGCTGAGTGGATTTGGCCAGTCAGGAAGCTTCTGCCGGTTAGCT
NM_014324.5         AGGCTTATTTATGCCAGGCTGAGTGGATTTGGCCAGTCAGGAAGCTTCTGCCGGTTAGCT
                    ************************************************************

HsCD00081865        GGCCACGATATCAACTATTTGGCTTTGTCAG-----------------------------
NM_203382.2         GGCCACGATATCAACTATTTGGCTTTGTCAG-----------------------------
NM_001167595.1      GGCCACGATATCAACTATTTGGCTTTGTCAGGTGTTCTCTCAAAAATTGGCAGAAGTGGT
NM_014324.5         GGCCACGATATCAACTATTTGGCTTTGTCAGGTGTTCTCTCAAAAATTGGCAGAAGTGGT
                    *******************************                             

HsCD00081865        ------------------------------------------------------------
NM_203382.2         ------------------------------------------------------------
NM_001167595.1      GAGAATCCGTATGCCCCGCTGAATCTCCTGGCTGACTTTGCTGGTGGTGGCCTTATGTGT
NM_014324.5         GAGAATCCGTATGCCCCGCTGAATCTCCTGGCTGACTTTGCTGGTGGTGGCCTTATGTGT
                                                                                

HsCD00081865        ------------------------------------------------------------
NM_203382.2         ------------------------------------------------------------
NM_001167595.1      GCACTGGGCATTATAATGGCTCTTTTTGACCGCACACGCACTGGCAAGGGTCAGGTCATT
NM_014324.5         GCACTGGGCATTATAATGGCTCTTTTTGACCGCACACGCACTGGCAAGGGTCAGGTCATT
                                                                                

HsCD00081865        ------------GTGGAAGGAACAGCATATTTAAGTTCTTTTCTGTGGAAAACTCAGAAA
NM_203382.2         ------------GTGGAAGGAACAGCATATTTAAGTTCTTTTCTGTGGAAAACTCAGAAA
NM_001167595.1      GATGCAAATATGGTGGAAGGAACAGCATATTTAAGTTCTTTTCTGTGGAAAACTCAGAAA
NM_014324.5         GATGCAAATATGGTGGAAGGAACAGCATATTTAAGTTCTTTTCTGTGGAAAACTCAGAAA
                                ************************************************

HsCD00081865        TCGAGTCTGTGGGAAGCACCTCGAGGACAGAACATGTTGGATGGTGGAGCACCTTTCTAT
NM_203382.2         TTGAGTCTGTGGGAAGCACCTCGAGGACAGAACATGTTGGATGGTGGAGCACCTTTCTAT
NM_001167595.1      TTGAGTCTGTGGGAAGCACCTCGAGGACAGAACATGTTGGATGGTGGAGCACCTTTCTAT
NM_014324.5         TTGAGTCTGTGGGAAGCACCTCGAGGACAGAACATGTTGGATGGTGGAGCACCTTTCTAT
                    * **********************************************************

HsCD00081865        ACGACTTACAGGACAGCAGATGGGGAATTCATGGCTGTTGGAGCAATAGAACCCCAGTTC
NM_203382.2         ACGACTTACAGGACAGCAGATGGGGAATTCATGGCTGTTGGAGCAATAGAACCCCAGTTC
NM_001167595.1      ACGACTTACAGGACAGCAGATGGGGAATTCATGGCTGTTGGAGCAATAGAACCCCAGTTC
NM_014324.5         ACGACTTACAGGACAGCAGATGGGGAATTCATGGCTGTTGGAGCAATAGAACCCCAGTTC
                    ************************************************************

HsCD00081865        TACGAGCTGCTGATCAAAGGACTTGGACTAAAGTC-------------------------
NM_203382.2         TACGAGCTGCTGATCAAAGGACTTGGACTAAAGTCTGA----------------------
NM_001167595.1      TACGAGCTGCTGATCAAAGGACTTGGACTAAAGTCTGATGAACTTCCCAATCAGATGAGC
NM_014324.5         TACGAGCTGCTGATCAAAGGACTTGGACTAAAGTCTGATGAACTTCCCAATCAGATGAGC
                    ***********************************                         

HsCD00081865        ------------------------------------------------------------
NM_203382.2         ------------------------------------------------------------
NM_001167595.1      ATGGATGATTGGCCAGAAATGAAGAAGAAGTTTGCAGATGTATTTGCAGAGAAGACGAAG
NM_014324.5         ATGGATGATTGGCCAGAAATGAAGAAGAAGTTTGCAGATGTATTTGCAGAGAAGACGAAG
                                                                                

HsCD00081865        ------------------------------------------------------------
NM_203382.2         ------------------------------------------------------------
NM_001167595.1      GCAGAGTGGTGTCAAATCTTTGACGGCACAGATGCCTGTGTGACTCCGGTTCTGACTTTT
NM_014324.5         GCAGAGTGGTGTCAAATCTTTGACGGCACAGATGCCTGTGTGACTCCGGTTCTGACTTTT
                                                                                

HsCD00081865        ------------------------------------------------------------
NM_203382.2         ------------------------------------------------------------
NM_001167595.1      GAGGAGGTTGTTCATCATGATCACAACAAGGAACGGGGCTCGTTTATCACCAGTGAGGAG
NM_014324.5         GAGGAGGTTGTTCATCATGATCACAACAAGGAACGGGGCTCGTTTATCACCAGTGAGGAG
                                                                                

HsCD00081865        ------------------------------------------------------------
NM_203382.2         ------------------------------------------------------------
NM_001167595.1      CAGGACGTGAGCCCCCGCCCTGCACCTCTGCTGTTAAACACCCCAGCCATCCCTTCTTTC
NM_014324.5         CAGGACGTGAGCCCCCGCCCTGCACCTCTGCTGTTAAACACCCCAGCCATCCCTTCTTTC
                                                                                

HsCD00081865        ------------------------------------------------------------
NM_203382.2         ------------------------------------------------------------
NM_001167595.1      AAAAGGGATCCTTTCATAGGAGAACACACTGAGGAGATACTTGAAGAATTTGGATTCAGC
NM_014324.5         AAAAGGGATCCTTTCATAGGAGAACACACTGAGGAGATACTTGAAGAATTTGGATTCAGC
                                                                                

HsCD00081865        ------------------------------------------------------------
NM_203382.2         ------------------------------------------------------------
NM_001167595.1      CGCGAAGAGATTTATCAGCTTAACTCAGATAAAATCATTGAAAGTAATAAGGCTGGTAGC
NM_014324.5         CGCGAAGAGATTTATCAGCTTAACTCAGATAAAATCATTGAAAGTAATAAGGTAAAAGCT
                                                                                

HsCD00081865        ---------------------------------------------
NM_203382.2         ---------------------------------------------
NM_001167595.1      AAGTTCTGGATCTTATACCCAACACACAGCAACATCCAGAAATAA
NM_014324.5         AGTCTCTAA------------------------------------
                                                                 

Pathways

Reactome : Beta-oxidation of pristanoyl-CoA
Reactome : Bile acid and bile salt metabolism
Reactome : Metabolism
Reactome : Metabolism of lipids and lipoproteins
Reactome : Peroxisomal lipid metabolism
Reactome : Synthesis of bile acids and bile salts
Reactome : Synthesis of bile acids and bile salts via 24-hydroxycholesterol
Reactome : Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol

Protein

UniProt : Q3KT79
UniProt : Q9UHK6
UniProt : E9PGA5
HPRD : 05134

Clone

Cloning Information : 266489
HIP Master Clone ID : 198587
IMAGE ID : 100002578
Original Clone ID : FLH266489.01L

Organism


TAX_ID : 9606
Species Specific ID: 23600

Genome

Chromosome : 5
Map Location : 5p13
Ensembl : ENSG00000242110

Reagents

Labome : AMACR-antibody