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LYSMD3 (Homo sapiens) in pDONR201 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  LYSMD3
Gene Name:  LysM, putative peptidoglycan-binding, domain containing 3
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH266641.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR201               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: The ORFeome Collaboration
HsCD00082017

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 918nts        
Open reading frame : 1 to 918
Reference Sequence Alignment

ATGGCAGGGAGGCATCAGAATCGTAGTTTTCCTCTTCCAGGAGTTCAGTCAAGTGGTCAA
GTACATGCATTTGGAAATTGTTCAGACAGTGATATTTTGGAGGAGGATGCTGAAGTGTAT
GAGCTTCGATCCAGAGGAAAAGAGAAAGTCCGAAGAAGTACATCAAGAGATAGACTTGAC
GACATTATAGTATTAACAAAAGATATACAAGAAGGAGATACATTAAATGCAATAGCCCTT
CAGTACTGTTGTACGGTAGCAGATATCAAGAGAGTTAACAATCTCATCAGTGATCAAGAC
TTTTTTGCCCTTAGGTCTATCAAAATTCCAGTAAAAAAGTTCAGTTCCTTGACCGAAACA
CTTTGTCCTCCAAAAGGAAGACAGACTTCACGTCATTCATCTGTTCAATACTCTTCCGAA
CAACAGGAAATTTTGCCAGCTAATGATTCTCTTGCTTACAGTGACTCAGCTGGTAGCTTT
TTAAAAGAAGTAGACCGAGACATAGAACAAATAGTAAAGTGTACAGACAATAAGAGAGAG
AACCTCAATGAGGTAGTATCGGCCTTAACAGCACAACAAATGCGTTTTGAACCTGATAAC
AAAAACACTCAACGTAAAGACCCCTATTATGGAGCAGACTGGGGAATAGGGTGGTGGACA
GCTGTAGTGATAATGTTGATAGTAGGTATAATAACACCAGTGTTTTATTTGTTGTATTAT
GCAATTTTAGCTAAGGTGGATGTTAGTCATCATTCAACAGTGGACTCTTCACATTTACAT
TCAAAAATCACACCCCCATCACAGCAGAGAGAAATGGAAAATGGAATTGTGCCAACTAAA
GGAATACATTTCAGCCAACAAGATGATCATAAACTGTATAGTCAAGATTCTCAGTCACCT
GCTGCTCAACAGGAAACA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1069
Start on reference sequence 149
Mutations:
None
Discrepancy:
a123g; a722c,E241A
                                

5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR201

pDONR201 in advanced viewed

Synonyms: ''
Sequencing Primer: Forward:  pDONR201-forward
Reverse:  pDONR201-reverse
Description: Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4470
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 (pUC-type) origin of replication (modified, low copy number) 3744 4426
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1264 959
primer site forward primer recommended forward primer site 5 tcgcgttaacgctagcatggatctc 3 300 324
primer site reverse primer recommended reverse primer site 5 gtaacatcagagattttgagacac 3 2769 2792
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 332 563
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2744 2513
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 332 563
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2744 2513
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2265 2513
selectable marker KanR kanamycin resistance gene 2868 3677
trxn termination sequence rrn T2 rrn T2 transcription termination sequence 73 100
trxn termination sequence rrn T1 rrn T1 transcription termination sequence 232 275

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LYSMD3
Symbol Nomenclature : LYSMD3
Designation : lysM and putative peptidoglycan-binding domain-containing protein 3
Full Nomenclature : LysM, putative peptidoglycan-binding, domain containing 3
GENEID : 116068
GI : 117646377
GenBank Accession : AM393781
HGNC : 26969
Vega : OTTHUMG00000162667
Target GenBank: NM_198273

 

Reference Sequence Alignment

  

HsCD00082017      ATGGCAGGGAGGCATCAGAATCGTAGTTTTCCTCTTCCAGGAGTTCAGTCAAGTGGTCAA
NM_198273.2       ATGGCAGGGAGGCATCAGAATCGTAGTTTTCCTCTTCCAGGAGTTCAGTCAAGTGGTCAA
                  ************************************************************

HsCD00082017      GTACATGCATTTGGAAATTGTTCAGACAGTGATATTTTGGAGGAGGATGCTGAAGTGTAT
NM_198273.2       GTACATGCATTTGGAAATTGTTCAGACAGTGATATTTTGGAGGAGGATGCTGAAGTGTAT
                  ************************************************************

HsCD00082017      GAGCTTCGATCCAGAGGAAAAGAGAAAGTCCGAAGAAGTACATCAAGAGATAGACTTGAC
NM_198273.2       GAACTTCGATCCAGAGGAAAAGAGAAAGTCCGAAGAAGTACATCAAGAGATAGACTTGAC
                  **.*********************************************************

HsCD00082017      GACATTATAGTATTAACAAAAGATATACAAGAAGGAGATACATTAAATGCAATAGCCCTT
NM_198273.2       GACATTATAGTATTAACAAAAGATATACAAGAAGGAGATACATTAAATGCAATAGCCCTT
                  ************************************************************

HsCD00082017      CAGTACTGTTGTACGGTAGCAGATATCAAGAGAGTTAACAATCTCATCAGTGATCAAGAC
NM_198273.2       CAGTACTGTTGTACGGTAGCAGATATCAAGAGAGTTAACAATCTCATCAGTGATCAAGAC
                  ************************************************************

HsCD00082017      TTTTTTGCCCTTAGGTCTATCAAAATTCCAGTAAAAAAGTTCAGTTCCTTGACCGAAACA
NM_198273.2       TTTTTTGCCCTTAGGTCTATCAAAATTCCAGTAAAAAAGTTCAGTTCCTTGACCGAAACA
                  ************************************************************

HsCD00082017      CTTTGTCCTCCAAAAGGAAGACAGACTTCACGTCATTCATCTGTTCAATACTCTTCCGAA
NM_198273.2       CTTTGTCCTCCAAAAGGAAGACAGACTTCACGTCATTCATCTGTTCAATACTCTTCCGAA
                  ************************************************************

HsCD00082017      CAACAGGAAATTTTGCCAGCTAATGATTCTCTTGCTTACAGTGACTCAGCTGGTAGCTTT
NM_198273.2       CAACAGGAAATTTTGCCAGCTAATGATTCTCTTGCTTACAGTGACTCAGCTGGTAGCTTT
                  ************************************************************

HsCD00082017      TTAAAAGAAGTAGACCGAGACATAGAACAAATAGTAAAGTGTACAGACAATAAGAGAGAG
NM_198273.2       TTAAAAGAAGTAGACCGAGACATAGAACAAATAGTAAAGTGTACAGACAATAAGAGAGAG
                  ************************************************************

HsCD00082017      AACCTCAATGAGGTAGTATCGGCCTTAACAGCACAACAAATGCGTTTTGAACCTGATAAC
NM_198273.2       AACCTCAATGAGGTAGTATCGGCCTTAACAGCACAACAAATGCGTTTTGAACCTGATAAC
                  ************************************************************

HsCD00082017      AAAAACACTCAACGTAAAGACCCCTATTATGGAGCAGACTGGGGAATAGGGTGGTGGACA
NM_198273.2       AAAAACACTCAACGTAAAGACCCCTATTATGGAGCAGACTGGGGAATAGGGTGGTGGACA
                  ************************************************************

HsCD00082017      GCTGTAGTGATAATGTTGATAGTAGGTATAATAACACCAGTGTTTTATTTGTTGTATTAT
NM_198273.2       GCTGTAGTGATAATGTTGATAGTAGGTATAATAACACCAGTGTTTTATTTGTTGTATTAT
                  ************************************************************

HsCD00082017      GCAATTTTAGCTAAGGTGGATGTTAGTCATCATTCAACAGTGGACTCTTCACATTTACAT
NM_198273.2       GAAATTTTAGCTAAGGTGGATGTTAGTCATCATTCAACAGTGGACTCTTCACATTTACAT
                  *.**********************************************************

HsCD00082017      TCAAAAATCACACCCCCATCACAGCAGAGAGAAATGGAAAATGGAATTGTGCCAACTAAA
NM_198273.2       TCAAAAATCACACCCCCATCACAGCAGAGAGAAATGGAAAATGGAATTGTGCCAACTAAA
                  ************************************************************

HsCD00082017      GGAATACATTTCAGCCAACAAGATGATCATAAACTGTATAGTCAAGATTCTCAGTCACCT
NM_198273.2       GGAATACATTTCAGCCAACAAGATGATCATAAACTGTATAGTCAAGATTCTCAGTCACCT
                  ************************************************************

HsCD00082017      GCTGCTCAACAGGAAACA---
NM_198273.2       GCTGCTCAACAGGAAACATAG
                  ******************   

Protein

SMART domain : LysM : Lysin motif
UniProt : A8K613
UniProt : Q7Z3D4

Clone

Cloning Information : 266641
HIP Master Clone ID : 198739
IMAGE ID : 100001964
Original Clone ID : FLH266641.01L

Organism


TAX_ID : 9606
Species Specific ID: 116068

Genome

Chromosome : 5
Map Location : 5q14.3
Ensembl : ENSG00000176018

Reagents

Labome : LYSMD3-antibody