EOMES (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  EOMES
Gene Name:  eomesodermin
Original Clone ID:
ORFeome_LaBaer-003.0129
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00820851
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2115nts
Open reading frame: 1 to 2115

ATGCAGTTAGGGGAGCAGCTCTTGGTGAGCTCAGTAAACCTGCCAGGAGCGCACTTTTAT
CCGCTTGAGAGTGCGCGAGGCGGAAGCGGAGGAAGCGCTGGCCACCTCCCCAGCGCGGCT
CCTTCTCCTCAGAAGTTGGACTTAGACAAAGCGTCCAAGAAGTTTTCCGGCAGTCTCTCC
TGCGAGGCGGTTAGCGGGGAGCCAGCAGCCGCCAGCGCAGGAGCTCCTGCTGCCATGCTT
AGTGACACAGACGCTGGAGATGCATTTGCTAGTGCTGCGGCAGTGGCCAAGCCGGGACCA
CCGGATGGACGAAAAGGATCCCCTTGTGGAGAAGAGGAACTTCCTTCTGCTGCTGCAGCC
GCTGCCGCTGCTGCTGCCGCGGCTGCTGCTACTGCTCGATACTCCATGGACAGCCTTAGT
TCCGAGCGGTACTACCTACAGTCCCCCGGTCCTCAGGGGTCTGAGTTAGCTGCTCCCTGC
TCACTCTTTCCTTATCAGGCGGCGGCTGGGGCGCCCCATGGACCTGTGTACCCTGCTCCT
AATGGGGCTCGATATCCTTACGGATCCATGCTTCCCCCTGGAGGCTTCCCTGCTGCTGTT
TGCCCACCTGGAAGGGCTCAGTTCGGACCAGGAGCTGGTGCGGGAAGTGGAGCAGGCGGT
TCTAGTGGAGGAGGCGGAGGACCTGGAACCTATCAGTACAGCCAGGGAGCTCCGCTTTAC
GGGCCTTACCCTGGAGCCGCAGCAGCTGGATCTTGTGGAGGACTGGGAGGCCTTGGGGTT
CCAGGTTCTGGCTTCCGTGCCCACGTCTACCTGTGCAACCGGCCTCTGTGGCTCAAATTC
CACCGCCACCAAACTGAGATGATCATTACGAAACAGGGCAGGCGCATGTTTCCTTTCTTG
AGCTTCAACATAAACGGACTCAATCCCACTGCCCACTACAATGTGTTCGTAGAGGTGGTG
CTGGCGGACCCCAACCACTGGCGCTTCCAAGGGGGCAAATGGGTGACCTGTGGCAAAGCC
GACAATAACATGCAGGGCAACAAAATGTATGTTCACCCAGAGTCTCCTAATACTGGTTCC
CACTGGATGAGACAGGAGATTTCATTCGGGAAATTAAAACTCACCAATAACAAAGGCGCA
AATAACAACAACACCCAGATGATAGTCTTACAATCCTTACACAAATACCAACCCCGACTG
CATATTGTTGAAGTTACAGAGGATGGCGTGGAGGACTTGAATGAGCCCTCAAAGACCCAG
ACTTTTACCTTCTCAGAAACGCAATTCATCGCAGTGACTGCCTACCAAAACACCGATATT
ACTCAACTAAAGATTGATCATAACCCCTTTGCAAAAGGCTTCAGAGACAACTATGATTCC
ATGTACACCGCTTCAGAAAATGACAGGTTAACTCCATCTCCCACGGATTCTCCTAGATCC
CATCAGATTGTCCCTGGAGGTCGGTACGGCGTTCAATCCTTCTTCCCGGAGCCCTTTGTC
AACACTTTACCTCAAGCCCGCTATTATAATGGCGAGAGAACCGTGCCACAGACCAACGGC
CTCCTTTCACCCCAACAGAGCGAAGAGGTGGCCAACCCTCCCCAGCGGTGGCTTGTCACG
CCTGTCCAGCAACCTGGGACCAACAAACTAGACATCAGTTCCTATGAATCTGAATATACT
TCTAGCACATTGCTCCCATATGGCATTAAATCCTTGCCCCTTCAGACATCCCATGCCCTG
GGGTATTACCCAGACCCAACCTTTCCTGCAATGGCAGGGTGGGGAGGTCGAGGTTCTTAC
CAGAGGAAGATGGCAGCTGGACTACCATGGACCTCCAGAACAAGCCCCACTGTCTTCTCT
GAAGATCAGCTCTCCAAGGAGAAAGTGAAAGAGGAAATTGGCTCTTCTTGGATAGAGACA
CCCCCTTCCATCAAATCTCTAGATTCCAATGATTCAGGAGTATACACCAGTGCTTGTAAG
CGAAGGCGGCTGTCTCCTAGCAACTCCAGTAATGAAAATTCACCCTCCATAAAGTGTGAG
GACATTAATGCTGAAGAGTATAGTAAAGACACCTCAAAAGGCATGGGAGGGTATTATGCT
TTTTACACAACTCCC

Protein sequence predicted based on nucleotide sequence

MQLGEQLLVSSVNLPGAHFYPLESARGGSGGSAGHLPSAAPSPQKLDLDKASKKFSGSLS
CEAVSGEPAAASAGAPAAMLSDTDAGDAFASAAAVAKPGPPDGRKGSPCGEEELPSAAAA
AAAAAAAAAATARYSMDSLSSERYYLQSPGPQGSELAAPCSLFPYQAAAGAPHGPVYPAP
NGARYPYGSMLPPGGFPAAVCPPGRAQFGPGAGAGSGAGGSSGGGGGPGTYQYSQGAPLY
GPYPGAAAAGSCGGLGGLGVPGSGFRAHVYLCNRPLWLKFHRHQTEMIITKQGRRMFPFL
SFNINGLNPTAHYNVFVEVVLADPNHWRFQGGKWVTCGKADNNMQGNKMYVHPESPNTGS
HWMRQEISFGKLKLTNNKGANNNNTQMIVLQSLHKYQPRLHIVEVTEDGVEDLNEPSKTQ
TFTFSETQFIAVTAYQNTDITQLKIDHNPFAKGFRDNYDSMYTASENDRLTPSPTDSPRS
HQIVPGGRYGVQSFFPEPFVNTLPQARYYNGERTVPQTNGLLSPQQSEEVANPPQRWLVT
PVQQPGTNKLDISSYESEYTSSTLLPYGIKSLPLQTSHALGYYPDPTFPAMAGWGGRGSY
QRKMAAGLPWTSRTSPTVFSEDQLSKEKVKEEIGSSWIETPPSIKSLDSNDSGVYTSACK
RRRLSPSNSSNENSPSIKCEDINAEEYSKDTSKGMGGYYAFYTTP

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)
3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable