Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
ORFeome_LaBaer-011.0098
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00821282
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5070nts
Open reading frame: 1 to 5070
ATGGCGGGCGTCGGGCCCGGGGGCTACGCGGCCGAGTTCGTGCCACCGCCAGAGTGCCCC
GTCTTTGAGCCGAGTTGGGAGGAGTTCACAGATCCGCTAAGCTTTATCGGGAGAATCCGG
CCTTTGGCGGAGAAAACCGGCATCTGCAAAATCCGCCCGCCCAAGGACTGGCAGCCTCCA
TTTGCCTGTGAAGTAAAAAGCTTTCGTTTCACTCCAAGAGTCCAGCGGCTGAATGAACTT
GAGGCAATGACCAGAGTGAGATTGGATTTCTTGGATCAACTAGCAAAATTTTGGGAACTT
CAAGGATCTACTCTGAAGATCCCTGTGGTAGAGAGAAAAATCCTGGATCTGTATGCTCTA
AGCAAGATTGTTGCCAGCAAAGGAGGTTTTGAAATGGTCACAAAAGAGAAGAAATGGTCC
AAAGTGGGTAGCCGCTTGGGATATCTGCCAGGAAAAGGGACTGGCTCTCTTTTAAAGTCA
CATTATGAAAGAATTCTCTACCCATATGAGCTTTTCCAGTCCGGTGTGAGCCTTATGGGT
GTGCAGATGCCTAATTTAGATCTTAAAGAAAAAGTGGAGCCTGAGGTTCTCAGCACTGAT
ACCCAAACTTCCCCAGAGCCAGGCACAAGGATGAACATTCTGCCGAAGAGAACAAGGCGT
GTGAAGACTCAGTCAGAATCAGGAGATGTGAGTAGGAACACGGAACTGAAGAAACTTCAG
ATTTTTGGGGCTGGGCCCAAGGTTGTCGGCTTGGCAATGGGAACCAAAGATAAAGAAGAT
GAGGTCACCCGAAGACGAAAAGTTACCAACAGGTCAGACGCATTTAACATGCAAATGAGA
CAACGGAAAGGCACTCTCTCTGTTAACTTTGTTGATCTTTATGTTTGTATGTTTTGTGGT
CGGGGAAACAATGAAGATAAATTGCTTTTGTGTGATGGATGTGATGACTCCTATCACACA
TTTTGTCTAATTCCCCCACTACCTGATGTGCCCAAAGGAGACTGGAGGTGTCCTAAATGT
GTCGCCGAGGAATGTAGCAAACCTCGAGAAGCCTTTGGATTTGAACAAGCTGTACGAGAG
TATACACTTCAGAGCTTTGGCGAGATGGCAGATAATTTTAAGTCTGATTATTTTAATATG
CCAGTCCATATGGTTCCCACAGAACTAGTAGAAAAGGAATTTTGGCGGCTGGTAAGCAGC
ATTGAAGAAGATGTTATTGTGGAATATGGAGCAGATATCTCCTCAAAAGACTTTGGAAGT
GGATTTCCGGTTAAGGATGGGCGAAGAAAGATTCTGCCAGAAGAAGAGGAATATGCACTT
TCTGGTTGGAATTTGAATAACATGCCTGTCCTGGAACAGTCAGTGCTTGCACATATTAAT
GTGGACATCTCTGGTATGAAAGTGCCGTGGCTGTATGTGGGAATGTGCTTCTCGTCTTTT
TGCTGGCACATTGAGGATCACTGGAGTTATTCCATCAACTACCTCCACTGGGGCGAGCCA
AAGACATGGTATGGTGTGCCCTCTCATGCTGCAGAGCAACTGGAGGAGGTCATGAGAGAG
CTGGCCCCCGAGTTATTTGAATCCCAGCCTGATCTTCTGCATCAGTTAGTTACCATCATG
AACCCCAACGTGCTTATGGAGCATGGGGTGCCTGTGTACAGGACCAATCAGTGTGCTGGC
GAGTTTGTTGTGACATTTCCTCGTGCCTATCACTCTGGATTTAACCAGGGATACAACTTT
GCTGAAGCTGTGAACTTCTGTACTGCTGACTGGTTGCCCATTGGACGTCAATGTGTAAAT
CATTACCGACGCCTAAGGCGCCACTGTGTCTTTTCACACGAGGAACTAATTTTCAAGATG
GCAGCAGATCCAGAATGCTTAGATGTGGGGCTGGCTGCTATGGTCTGCAAAGAATTGACT
CTCATGACTGAAGAAGAAACACGATTAAGAGAGTCTGTTGTACAGATGGGTGTCCTGATG
TCAGAAGAAGAAGTGTTTGAACTTGTTCCTGATGATGAGCGGCAGTGTTCAGCATGCAGA
ACCACATGTTTTCTCTCTGCTCTCACATGTTCCTGTAATCCCGAGCGGCTTGTATGTCTC
TACCATCCAACTGATCTGTGCCCCTGCCCCATGCAGAAGAAATGTCTTAGATATCGCTAC
CCATTAGAAGACCTCCCTTCTCTGCTCTATGGTGTAAAAGTCAGGGCACAGTCCTATGAC
ACCTGGGTCAGTCGAGTTACAGAAGCATTGTCTGCTAACTTCAACCACAAAAAAGATTTA
ATTGAATTGCGAGTAATGCTGGAAGATGCTGAGGATAGGAAGTACCCAGAGAATGATCTC
TTTCGAAAACTCAGGGATGCTGTAAAAGAAGCCGAAACCTGTGCTTCTGTGGCTCAGCTG
TTACTGAGCAAAAAGCAGAAACACAGACAGAGCCCAGATAGTGGGAGAACTCGCACCAAA
CTGACAGTGGAAGAATTGAAGGCCTTTGTGCAACAACTGTTTAGTCTTCCGTGTGTCATC
AGCCAAGCTCGGCAAGTAAAGAATCTCCTAGATGATGTGGAAGAGTTTCATGAACGTGCT
CAGGAGGCCATGATGGATGAAACCCCAGATTCTTCCAAACTCCAGATGTTGATAGATATG
GGCTCTAGTCTCTATGTGGAACTGCCTGAATTACCAAGACTGAAGCAAGAGCTACAACAA
GCTCGGTGGTTGGACGAAGTAAGACTGACCTTATCAGATCCGCAACAAGTCACTTTGGAT
GTGATGAAGAAGCTGATAGACTCTGGGGTAGGGTTGGCACCCCACCATGCCGTGGAGAAA
GCAATGGCTGAACTACAGGAGCTCCTTACAGTCTCTGAACGATGGGAAGAAAAGGCTAAG
GTCTGCCTACAGGCAAGGCCGAGGCACAGCGTGGCAAGTTTAGAAAGCATTGTGAATGAA
GCCAAGAACATTCCAGCCTTTCTGCCCAATGTGTTGTCCTTGAAAGAAGCCCTACAAAAG
GCTCGAGAATGGACCGCTAAAGTGGAAGCTATTCAGAGTGGCAGCAATTACGCTTATTTG
GAGCAGCTCGAGAGCTTGTCTGCGAAAGGACGGCCTATTCCTGTGCGTCTTGAAGCACTG
CCGCAAGTGGAATCTCAGGTAGCAGCAGCACGGGCATGGAGAGAACGGACTGGGCGGACG
TTTCTTAAGAAGAATTCGAGCCATACATTGTTACAGGTGCTGAGCCCCAGGACCGACATT
GGTGTATATGGGAGTGGCAAAAATAGGAGGAAAAAAGTAAAAGAACTAATAGAAAAAGAA
AAAGAAAAGGATCTGGACCTGGAGCCTCTGAGCGATCTCGAGGAAGGACTGGAGGAAACC
AGAGATACAGCCATGGTGGTGGCAGTTTTCAAAGAACGGGAGCAAAAAGAGATTGAAGCC
ATGCATTCTCTGAGAGCAGCCAACCTAGCCAAGATGACAATGGTGGACCGCATAGAAGAA
GTAAAATTTTGCATTTGCCGCAAGACAGCCAGTGGGTTTATGCTACAGTGTGAGCTCTGC
AAAGACTGGTTCCATAACAGCTGTGTTCCTCTTCCTAAATCAAGTAGCCAAAAAAAAGGC
TCCAGCTGGCAAGCTAAGGAAGTAAAATTCCTTTGCCCTCTTTGTATGCGGTCCCGACGA
CCCAGGCTAGAGACTATTCTGTCTCTCCTGGTATCCCTTCAGAAGTTGCCCGTACGGTTG
CCTGAAGGAGAGGCCCTGCAGTGTTTGACAGAACGTGCTATGAGTTGGCAAGATAGAGCG
CGCCAGGCTCTAGCCACAGATGAACTATCCTCTGCCCTGGCCAAACTGTCTGTGTTGAGT
CAGCGTATGGTGGAACAGGCGGCTCGAGAAAAAACTGAAAAGATCATCAGTGCAGAACTC
CAAAAAGCAGCTGCTAATCCAGATTTACAGGGCCACTTACCTAGTTTCCAGCAGTCTGCT
TTTAACAGGGTGGTGAGCAGTGTGTCATCTTCTCCTCGACAAACAATGGACTATGATGAT
GAAGAAACAGACTCTGATGAAGACATTCGAGAGACATATGGCTACGACATGAAGGACACC
GCTTCTGTGAAGTCTTCTAGTAGTCTTGAACCCAATCTTTTTTGTGATGAAGAGATTCCC
ATCAAATCCGAGGAGGTGGTGACCCACATGTGGACAGCACCTTCATTTTGTGCAGAGCAT
GCTTATTCCTCTGCTTCTAAAAGTTGTTCTCAAGGTTCTAGCACCCCAAGGAAACAACCT
CGGAAGAGCCCTTTGGTGCCCCGAAGTTTGGAACCCCCAGTGCTGGAGTTGTCACCTGGA
GCTAAGGCACAACTCGAAGAACTTATGATGGTTGGAGATCTCCTGGAAGTATCTCTGGAC
GAGACTCAACACATATGGCGGATTCTCCAGGCCACACACCCACCCTCTGAAGACAGATTC
TTGCATATCATGGAGGATGACAGCATGGAAGAGAAACCACTAAAAGTGAAAGGAAAGGAC
TCTTCAGAGAAGAAACGGAAACGGAAGCTGGAAAAGGTAGAGCAACTTTTTGGAGAAGGA
AAACAGAAGTCCAAGGAGTTAAAGAAAATGGACAAACCTAGAAAGAAGAAATTAAAATTA
GGTGCAGACAAATCAAAGGAGCTGAATAAACTCGCCAAGAAACTAGCAAAAGAAGAAGAG
AGGAAGAAAAAGAAGGAGAAGGCTGCTGCAGCCAAAGTTGAACTTGTGAAAGAGAGCACT
GAAAAGAAAAGAGAGAAAAAGGTGCTGGACATCCCCTCGAAGTATGACTGGTCAGGAGCA
GAGGAGTCTGATGATGAGAATGCTGTGTGCGCCGCACAGAACTGCCAAAGGCCCTGCAAG
GACAAGGTCGACTGGGTCCAATGTGATGGTGGCTGTGATGAGTGGTTTCATCAAGTTTGT
GTGGGTGTATCTCCAGAAATGGCTGAAAATGAAGATTACATCTGTATAAACTGTGCAAAG
AAGCAGGGGCCAGTTAGCCCAGGTCCAGCACCACCTCCTTCCTTCATAATGAGCTACAAA
CTACCCATGGAGGATCTTAAAGAAACCAGT
Protein sequence predicted based on nucleotide sequence
MAGVGPGGYAAEFVPPPECPVFEPSWEEFTDPLSFIGRIRPLAEKTGICKIRPPKDWQPP FACEVKSFRFTPRVQRLNELEAMTRVRLDFLDQLAKFWELQGSTLKIPVVERKILDLYAL SKIVASKGGFEMVTKEKKWSKVGSRLGYLPGKGTGSLLKSHYERILYPYELFQSGVSLMG VQMPNLDLKEKVEPEVLSTDTQTSPEPGTRMNILPKRTRRVKTQSESGDVSRNTELKKLQ IFGAGPKVVGLAMGTKDKEDEVTRRRKVTNRSDAFNMQMRQRKGTLSVNFVDLYVCMFCG RGNNEDKLLLCDGCDDSYHTFCLIPPLPDVPKGDWRCPKCVAEECSKPREAFGFEQAVRE YTLQSFGEMADNFKSDYFNMPVHMVPTELVEKEFWRLVSSIEEDVIVEYGADISSKDFGS GFPVKDGRRKILPEEEEYALSGWNLNNMPVLEQSVLAHINVDISGMKVPWLYVGMCFSSF CWHIEDHWSYSINYLHWGEPKTWYGVPSHAAEQLEEVMRELAPELFESQPDLLHQLVTIM NPNVLMEHGVPVYRTNQCAGEFVVTFPRAYHSGFNQGYNFAEAVNFCTADWLPIGRQCVN HYRRLRRHCVFSHEELIFKMAADPECLDVGLAAMVCKELTLMTEEETRLRESVVQMGVLM SEEEVFELVPDDERQCSACRTTCFLSALTCSCNPERLVCLYHPTDLCPCPMQKKCLRYRY PLEDLPSLLYGVKVRAQSYDTWVSRVTEALSANFNHKKDLIELRVMLEDAEDRKYPENDL FRKLRDAVKEAETCASVAQLLLSKKQKHRQSPDSGRTRTKLTVEELKAFVQQLFSLPCVI SQARQVKNLLDDVEEFHERAQEAMMDETPDSSKLQMLIDMGSSLYVELPELPRLKQELQQ ARWLDEVRLTLSDPQQVTLDVMKKLIDSGVGLAPHHAVEKAMAELQELLTVSERWEEKAK VCLQARPRHSVASLESIVNEAKNIPAFLPNVLSLKEALQKAREWTAKVEAIQSGSNYAYL EQLESLSAKGRPIPVRLEALPQVESQVAAARAWRERTGRTFLKKNSSHTLLQVLSPRTDI GVYGSGKNRRKKVKELIEKEKEKDLDLEPLSDLEEGLEETRDTAMVVAVFKEREQKEIEA MHSLRAANLAKMTMVDRIEEVKFCICRKTASGFMLQCELCKDWFHNSCVPLPKSSSQKKG SSWQAKEVKFLCPLCMRSRRPRLETILSLLVSLQKLPVRLPEGEALQCLTERAMSWQDRA RQALATDELSSALAKLSVLSQRMVEQAAREKTEKIISAELQKAAANPDLQGHLPSFQQSA FNRVVSSVSSSPRQTMDYDDEETDSDEDIRETYGYDMKDTASVKSSSSLEPNLFCDEEIP IKSEEVVTHMWTAPSFCAEHAYSSASKSCSQGSSTPRKQPRKSPLVPRSLEPPVLELSPG AKAQLEELMMVGDLLEVSLDETQHIWRILQATHPPSEDRFLHIMEDDSMEEKPLKVKGKD SSEKKRKRKLEKVEQLFGEGKQKSKELKKMDKPRKKKLKLGADKSKELNKLAKKLAKEEE RKKKKEKAAAAKVELVKESTEKKREKKVLDIPSKYDWSGAEESDDENAVCAAQNCQRPCK DKVDWVQCDGGCDEWFHQVCVGVSPEMAENEDYICINCAKKQGPVSPGPAPPPSFIMSYK LPMEDLKETS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
