Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
ORFeome_LaBaer-005.0088
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00821822
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6408nts
Open reading frame: 1 to 6408
ATGTCTCGATCCCGGCATGCAAGGCCTTCCAGATTAGTCAGGAAGGAAGATGTGAACAAA
AAAAAGAAAAACAGCCAACTACGAAAGACAACCAAGGGAGCCAACAAAAATGTGGCATCA
GTCAAGACTTTGAGCCCTGGAAAATTAAAGCAATTAATTCAAGAAAGAGATGTTAAGAAA
AAAACAGAACCTAAACCACCCGTGCCAGTCAGAAGCCTCCTGACAAGAGCTGGAGCAGCA
CGCATGAATTTGGATAGGACTGAGGTTCTTTTTCAGAACCCAGAGTCCTTAACCTGCAAT
GGGTTTACAATGGCGCTACGAAGCACCTCCCTTAGCAGACGACTCTCCCAACCCCCACTG
GTCGTAGCCAAATCCAAAAAGGTTCCACTTTCTAAGGGTTTAGAAAAGCAACATGATTGT
GATTATAAGATACTCCCTGCTTTGGGAGTAAAGCACTCAGAAAATGATTCGGTTCCAATG
CAAGACACCCAAGTCCTTCCTGATATAGAGACACTAATTGGTGTACAAAATCCCTCTTTA
CTTAAAGGTAAGAGCCAAGAGACAACTCAGTTTTGGTCCCAAAGAGTTGAGGATTCCAAG
ATCAATATCCCTACCCACAGTGGCCCTGCAGCTGAGATCCTTCCTGGGCCACTGGAAGGG
ACACGCTGTGGTGAAGGACTATTCTCTGAAGAGACATTGAATGATACCAGTGGTTCCCCA
AAAATGTTTGCTCAGGACACAGTGTGTGCTCCTTTTCCCCAAAGAGCAACCCCCAAAGTT
ACCTCTCAAGGAAACCCCAGCATTCAGTTAGAAGAGTTGGGTTCACGAGTAGAATCTCTT
AAGTTATCTGATTCTTACCTGGATCCCATTAAAAGTGAACATGATTGCTACCCCACCTCC
AGTCTTAATAAGGTTATACCTGACTTGAACCTTAGAAACTGCTTGGCTCTTGGTGGGTCT
ACGTCTCCTACCTCTGTAATAAAATTCCTCTTGGCAGGCTCAAAACAAGCGACCCTTGGT
GCTAAACCAGATCATCAAGAGGCCTTCGAAGCTACTGCAAATCAACAGGAAGTTTCTGAT
ACCACCTCTTTCCTAGGACAGGCCTTTGGTGCTATCCCACATCAATGGGAACTTCCTGGT
GCTGACCCAGTTCATGGTGAGGCCCTGGGTGAAACCCCAGATCTACCAGAGATTCCTGGT
GCTATTCCAGTCCAAGGAGAGGTCTTTGGTACTATTTTAGACCAACAAGAAACTCTTGGT
ATGAGTGGGAGTGTTGTCCCAGACTTGCCTGTCTTCCTTCCTGTTCCTCCAAATCCAATT
GCTACCTTTAATGCTCCTTCCAAATGGCCTGAGCCCCAAAGTACTGTCTCATATGGACTT
GCAGTCCAGGGTGCTATACAGATTTTGCCTTTGGGCTCAGGACACACTCCTCAATCATCA
TCAAACTCAGAGAAAAATTCATTACCTCCAGTAATGGCTATAAGCAATGTAGAAAATGAG
AAGCAGGTTCATATAAGCTTCCTGCCAGCTAACACTCAGGGGTTCCCATTAGCCCCTGAG
AGAGGACTCTTCCATGCTTCACTGGGTATAGCCCAACTCTCTCAGGCTGGTCCTAGCAAA
TCAGACAGAGGGAGCTCTCAGGTCAGTGTAACCAGCACAGTTCATGTTGTCAACACCACA
GTGGTGACTATGCCAGTGCCAATGGTCAGTACCTCCTCTTCTTCCTATACCACTTTGCTA
CCGACTTTGGAAAAGAAGAAAAGAAAGCGATGTGGGGTCTGTGAACCCTGCCAGCAGAAG
ACCAACTGTGGTGAATGCACTTACTGCAAGAACAGAAAGAACAGCCATCAGATCTGTAAG
AAAAGAAAATGTGAGGAGCTGAAAAAGAAACCATCTGTTGTTGTGCCTCTGGAGGTTATA
AAGGAAAACAAGAGGCCCCAGAGGGAAAAGAAGCCCAAAGTTTTAAAGGCAGATTTTGAC
AACAAACCAGTAAATGGCCCCAAGTCAGAATCCATGGACTACAGTAGATGTGGTCATGGG
GAAGAACAAAAATTGGAATTGAACCCACATACTGTTGAAAATGTAACTAAAAATGAAGAC
AGCATGACAGGCATCGAGGTGGAGAAGTGGACACAAAACAAGAAATCACAGTTAACTGAT
CACGTGAAAGGAGATTTTAGTGCTAATGTCCCAGAAGCTGAAAAATCGAAAAACTCTGAA
GTTGACAAGAAACGAACCAAATCTCCAAAATTGTTTGTACAAACCGTAAGAAATGGCATT
AAACATGTACACTGTTTACCAGCTGAAACAAATGTGTCCTTCAAAAAGTTCAATATTGAA
GAGTTCGGCAAGACATTGGAAAACAATTCTTATAAGTTCCTAAAAGACACCGCAAACCAT
AAAAACGCTATGAGCTCTGTTGCTACTGATATGAGTTGTGATCATCTCAAGGGGAGAAGT
AACGTTTTAGTATTCCAGCAGCCTGGCTTTAACTGCAGTTCCATTCCACATTCTTCACAC
TCCATCATAAATCATCATGCTAGTATACACAATGAAGGTGATCAACCAAAAACTCCTGAG
AATATACCAAGTAAAGAACCAAAAGATGGATCACCCGTTCAACCAAGTCTCTTATCGTTA
ATGAAAGATAGGAGATTAACATTGGAGCAAGTGGTAGCCATAGAGGCCCTGACTCAACTC
TCAGAAGCCCCATCAGAGAATTCCTCTCCATCAAAGTCAGAGAAGGATGAGGAATCAGAG
CAGAGAACAGCCAGTTTGCTTAATAGTTGCAAAGCTATCCTCTACACTGTAAGAAAAGAC
CTCCAAGACCCAAACTTACAGGGAGAGCCACCAAAACTTAATCACTGTCCATCTTTGGAA
AAACAAAGTTCATGCAACACGGTGGTTTTCAATGGGCAAACTACTACCCTTTCCAACTCA
CATATCAACTCAGCTACTAACCAAGCATCCACAAAGTCACATGAATATTCAAAGGTCACC
AATTCATTATCCCTTTTTATACCAAAATCCAATTCATCCAAGATTGACACCAATAAAAGT
ATTGCTCAGGGGATAATTACTCTTGACAATTGTTCCAATGATTTGCATCAGTTGCCACCA
AGAAATAATGAAGTGGAGTATTGCAACCAGTTACTGGACAGCAGCAAAAAATTGGACTCA
GATGATCTATCATGTCAGGATGCAACCCATACCCAAATTGAGGAAGATGTTGCAACACAG
TTGACACAACTTGCTTCTATAATTAAGATCAATTATATAAAACCAGAGGACAAAAAAGTT
GAAAGTACACCCACAAGCCTGGTCACATGTAATGTACAGCAAAAATACAATCAGGAGAAG
GGCACAATACAACAGAAACCACCTTCAAGTGTACACAATAATCATGGCTCATCATTAACA
AAACAAAAGAACCCAACCCAGAAAAAGACAAAATCCACCCCATCAAGAGATCGGCGGAAA
AAGAAGCCCACAGTTGTAAGTTATCAAGAAAATGATCGGCAGAAGTGGGAAAAGTTGTCC
TATATGTATGGCACAATATGCGACATTTGGATAGCATCGAAATTTCAAAATTTTGGGCAA
TTTTGTCCACATGATTTTCCTACTGTATTTGGGAAAATTTCTTCCTCGACCAAAATATGG
AAACCACTGGCTCAAACGAGGTCCATTATGCAACCCAAAACAGTATTTCCACCACTCACT
CAGATAAAATTACAGAGATATCCTGAATCAGCAGAGGAAAAGGTGAAGGTTGAACCATTG
GATTCACTCAGTTTATTTCATCTGAAAACGGAATCCAACGGGAAGGCATTCACTGATAAA
GCTTATAATTCTCAGGTACAGTTAACGGTGAATGCCAATCAGAAAGCCCATCCTTTGACC
CAGCCCTCTTCTCCACCTAACCAGTGTGCTAACGTGATGGCAGGCGATGACCAAATACGG
TTTCAGCAGGTTGTTAAGGAGCAACTCATGCATCAGCGACTGCCAACATTGCCTGGTATC
TCTCATGAAACACCCTTACCGGAGTCAGCACTAACTCTCAGGAATGTAAATGTAGTGTGT
TCAGGTGGAATTACAGTGGTTTCTACCAAAAGTGAAGAGGAAGTCTGTTCATCCAGTTTT
GGAACATCAGAATTTTCCACAGTGGACAGTGCACAGAAAAATTTTAATGATTATGCCATG
AACTTCTTTACTAACCCTACAAAAAACCTAGTGTCTATAACTAAAGATTCTGAACTCCCC
ACCTGTAGCTGTCTTGATCGAGTTATACAAAAAGACAAAGGCCCATATTATACACACCTT
GGGGCAGGACCAAGTGTTGCTGCTGTCAGGGAAATCATGGAGAATAGGTATGGTCAAAAA
GGAAACGCAATAAGGATAGAAATAGTAGTGTACACCGGTAAAGAAGGGAAAAGCTCTCAT
GGGTGTCCAATTGCTAAGTGGGTTTTAAGAAGAAGCAGTGATGAAGAAAAAGTTCTTTGT
CTGGTCCGGCAGCGTACAGGCCACCACTGTCCAACTGCTGTGATGGTGGTGCTCATCATG
GTGTGGGATGGCATCCCTCTTCCAATGGCCGACCGGCTATACACAGAGCTCACAGAGAAT
CTAAAGTCATACAATGGGCACCCTACCGACAGAAGATGCACCCTCAATGAAAATCGTACC
TGTACATGTCAAGGAATTGATCCAGAGACTTGTGGAGCTTCATTCTCTTTTGGCTGTTCC
TGGAGTATGTACTTTAATGGCTGTAAGTTTGGTAGAAGCCCAAGCCCCAGAAGATTTAGA
ATTGATCCAAGCTCTCCCTTGCATGAAAAAAACCTTGAAGATAACTTACAGAGTTTGGCT
ACACGATTAGCTCCAATCTATAAGCAGTATGCTCCAGTAGCTTACCAAAATCAGGTGGAA
TATGAAAATGTTGCCCGAGAATGTCGGCTTGGCAGCAAGGAAGGTCGTCCCTTCTCTGGG
GTCACTGCTTGCCTGGACTTCTGTGCTCATCCCCACAGGGACATTCACAACATGAATAAT
GGAAGCACTGTGGTTTGTACCTTAACTCGAGAAGATAACCGCTCTTTGGGTGTTATTCCT
CAAGATGAGCAGCTCCATGTGCTACCTCTTTATAAGCTTTCAGACACAGATGAGTTTGGC
TCCAAGGAAGGAATGGAAGCCAAGATCAAATCTGGGGCCATCGAGGTCCTGGCACCCCGC
CGCAAAAAAAGAACGTGTTTCACTCAGCCTGTTCCCCGTTCTGGAAAGAAGAGGGCTGCG
ATGATGACAGAGGTTCTTGCACATAAGATAAGGGCAGTGGAAAAGAAACCTATTCCTCGA
ATCAAGCGGAAGAATAACTCCACAACAACAAACAACAGTAAGCCTTCGTCACTGCCAACC
TTAGGGAGTAACACTGAGACTGTGCAACCTGAAGTAAAAAGTGAAACCGAACCCCATTTT
ATCTTAAAAAGTTCAGACAACACTAAAACTTATTCGCTGATGCCATCCGCTCCTCACCCA
GTGAAAGAGGCATCTCCAGGCTTCTCCTGGTCCCCGAAGACTGCTTCAGCCACACCAGCT
CCACTGAAGAATGACGCAACAGCCTCATGCGGGTTTTCAGAAAGGAGCAGCACTCCCCAC
TGTACGATGCCTTCGGGAAGACTCAGTGGTGCCAATGCAGCTGCTGCTGATGGCCCTGGC
ATTTCACAGCTTGGCGAAGTGGCTCCTCTCCCCACCCTGTCTGCTCCTGTGATGGAGCCC
CTCATTAATTCTGAGCCTTCCACCGGTGTGACTGAGCCGCTAACGCCTCATCAGCCAAAC
CACCAGCCCTCCTTCCTCACCTCTCCTCAAGACCTTGCCTCTTCTCCAATGGAAGAAGAT
GAGCAGCATTCTGAAGCAGATGAGCCTCCATCAGACGAACCCCTATCTGATGACCCCCTG
TCCCCTGCTGAGGAGAAATTGCCCCACATTGATGAGTATTGGTCAGACAGTGAGCACATC
TTTTTGGATGCAAATATTGGTGGGGTGGCCATCGCCCCTGCTCACGGCTCGGTTTTGATT
GAGTGTGCCCGGCGAGAGCTGCACGCTACCACTCCTGTTGAGCACCCCAACCGTAATCAT
CCAACCCGCCTCTCCCTTGTCTTTTACCAGCACAAAAACCTAAATAAGCCCCAACATGGT
TTTGAACTAAACAAGATTAAGTTTGAGGCTAAAGAAGCTAAGAATAAGAAAATGAAGGCC
TCAGAGCAAAAAGACCAGGCAGCTAATGAAGGTCCAGAACAGTCCTCCGAAGTAAATGAA
TTGAACCAAATTCCTTCTCATAAAGCATTAACATTAACCCATGACAATGTTGTCACCGTG
TCCCCTTATGCTCTCACACACGTTGCGGGGCCCTATAACCATTGGGTC
Protein sequence predicted based on nucleotide sequence
MSRSRHARPSRLVRKEDVNKKKKNSQLRKTTKGANKNVASVKTLSPGKLKQLIQERDVKK KTEPKPPVPVRSLLTRAGAARMNLDRTEVLFQNPESLTCNGFTMALRSTSLSRRLSQPPL VVAKSKKVPLSKGLEKQHDCDYKILPALGVKHSENDSVPMQDTQVLPDIETLIGVQNPSL LKGKSQETTQFWSQRVEDSKINIPTHSGPAAEILPGPLEGTRCGEGLFSEETLNDTSGSP KMFAQDTVCAPFPQRATPKVTSQGNPSIQLEELGSRVESLKLSDSYLDPIKSEHDCYPTS SLNKVIPDLNLRNCLALGGSTSPTSVIKFLLAGSKQATLGAKPDHQEAFEATANQQEVSD TTSFLGQAFGAIPHQWELPGADPVHGEALGETPDLPEIPGAIPVQGEVFGTILDQQETLG MSGSVVPDLPVFLPVPPNPIATFNAPSKWPEPQSTVSYGLAVQGAIQILPLGSGHTPQSS SNSEKNSLPPVMAISNVENEKQVHISFLPANTQGFPLAPERGLFHASLGIAQLSQAGPSK SDRGSSQVSVTSTVHVVNTTVVTMPVPMVSTSSSSYTTLLPTLEKKKRKRCGVCEPCQQK TNCGECTYCKNRKNSHQICKKRKCEELKKKPSVVVPLEVIKENKRPQREKKPKVLKADFD NKPVNGPKSESMDYSRCGHGEEQKLELNPHTVENVTKNEDSMTGIEVEKWTQNKKSQLTD HVKGDFSANVPEAEKSKNSEVDKKRTKSPKLFVQTVRNGIKHVHCLPAETNVSFKKFNIE EFGKTLENNSYKFLKDTANHKNAMSSVATDMSCDHLKGRSNVLVFQQPGFNCSSIPHSSH SIINHHASIHNEGDQPKTPENIPSKEPKDGSPVQPSLLSLMKDRRLTLEQVVAIEALTQL SEAPSENSSPSKSEKDEESEQRTASLLNSCKAILYTVRKDLQDPNLQGEPPKLNHCPSLE KQSSCNTVVFNGQTTTLSNSHINSATNQASTKSHEYSKVTNSLSLFIPKSNSSKIDTNKS IAQGIITLDNCSNDLHQLPPRNNEVEYCNQLLDSSKKLDSDDLSCQDATHTQIEEDVATQ LTQLASIIKINYIKPEDKKVESTPTSLVTCNVQQKYNQEKGTIQQKPPSSVHNNHGSSLT KQKNPTQKKTKSTPSRDRRKKKPTVVSYQENDRQKWEKLSYMYGTICDIWIASKFQNFGQ FCPHDFPTVFGKISSSTKIWKPLAQTRSIMQPKTVFPPLTQIKLQRYPESAEEKVKVEPL DSLSLFHLKTESNGKAFTDKAYNSQVQLTVNANQKAHPLTQPSSPPNQCANVMAGDDQIR FQQVVKEQLMHQRLPTLPGISHETPLPESALTLRNVNVVCSGGITVVSTKSEEEVCSSSF GTSEFSTVDSAQKNFNDYAMNFFTNPTKNLVSITKDSELPTCSCLDRVIQKDKGPYYTHL GAGPSVAAVREIMENRYGQKGNAIRIEIVVYTGKEGKSSHGCPIAKWVLRRSSDEEKVLC LVRQRTGHHCPTAVMVVLIMVWDGIPLPMADRLYTELTENLKSYNGHPTDRRCTLNENRT CTCQGIDPETCGASFSFGCSWSMYFNGCKFGRSPSPRRFRIDPSSPLHEKNLEDNLQSLA TRLAPIYKQYAPVAYQNQVEYENVARECRLGSKEGRPFSGVTACLDFCAHPHRDIHNMNN GSTVVCTLTREDNRSLGVIPQDEQLHVLPLYKLSDTDEFGSKEGMEAKIKSGAIEVLAPR RKKRTCFTQPVPRSGKKRAAMMTEVLAHKIRAVEKKPIPRIKRKNNSTTTNNSKPSSLPT LGSNTETVQPEVKSETEPHFILKSSDNTKTYSLMPSAPHPVKEASPGFSWSPKTASATPA PLKNDATASCGFSERSSTPHCTMPSGRLSGANAAAADGPGISQLGEVAPLPTLSAPVMEP LINSEPSTGVTEPLTPHQPNHQPSFLTSPQDLASSPMEEDEQHSEADEPPSDEPLSDDPL SPAEEKLPHIDEYWSDSEHIFLDANIGGVAIAPAHGSVLIECARRELHATTPVEHPNRNH PTRLSLVFYQHKNLNKPQHGFELNKIKFEAKEAKNKKMKASEQKDQAANEGPEQSSEVNE LNQIPSHKALTLTHDNVVTVSPYALTHVAGPYNHWV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
