Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
ORFeome_LaBaer-011.0116
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00821932
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5613nts
Open reading frame: 1 to 5613
ATGCCCTCTGTCTGCCTCCTTCTCCTGCTCTTCCTTGCTGTCGGCGGGGCCCTGGGCAAC
AGGCCTTTCCGTGCCTTCGTGGTGACCGACACCACCCTTACCCACCTGGCCGTGCACCGG
GTGACCGGGGAGGTGTTCGTGGGCGCAGTGAACCGAGTCTTTAAGCTGGCCCCCAACCTG
ACTGAGCTGCGGGCCCATGTCACGGGGCCCGTCGAGGACAACGCTCGCTGCTACCCGCCC
CCCAGCATGCGCGTGTGTGCCCACCGGCTCGCCCCCGTGGACAACATAAATAAGCTCCTG
CTCATAGACTATGCGGCCCGCCGCCTCGTGGCCTGCGGCAGCATCTGGCAGGGCATCTGC
CAGTTCCTGCGTCTGGACGACCTCTTCAAGCTTGGTGAGCCGCACCACCGCAAGGAGCAC
TACCTGTCGGGGGCCCAGGAGCCCGACTCCATGGCTGGTGTCATTGTGGAGCAGGGCCAG
GGGCCCAGCAAGCTGTTTGTGGGCACTGCCGTTGACGGCAAGTCGGAGTACTTCCCCACC
TTGAGCTCCCGCAAGCTTATCAGTGATGAAGACAGTGCGGACATGTTCAGTCTCGTGTAC
CAGGATGAGTTCGTATCCTCCCAGATCAAGATCCCATCAGACACGCTGTCCTTGTACCCT
GCCTTTGACATCTACTACATCTACGGCTTCGTCAGCGCCTCCTTCGTCTACTTCCTGACG
CTGCAGCTTGACACCCAGCAGACGCTGTTGGACACAGCGGGCGAGAAATTTTTCACGTCC
AAGATCGTGCGCATGTGCGCGGGAGACTCAGAGTTCTACTCATACGTGGAATTCCCCATC
GGATGCTCCTGGCGAGGAGTCGAGTACAGATTGGTGCAGAGCGCCCACTTGGCCAAACCT
GGACTGCTGCTGGCCCAGGCCCTGGGCGTGCCGGCTGATGAGGACGTGCTCTTCACCATC
TTCTCTCAGGGCCAGAAGAACCGGGCCAGCCCCCCCCGGCAGACCATCCTCTGCCTCTTC
ACCCTTAGCAACATCAATGCCCACATCCGGCGCCGCATCCAGTCCTGCTATCGTGGGGAG
GGCACTCTGGCTCTGCCCTGGCTGCTGAACAAGGAGCTGCCTTGCATCAACACCCCCATG
CAGATCAACGGCAACTTCTGTGGGCTGGTGCTTAACCAGCCTCTTGGAGGCCTGCATGTG
ATCGAGGGGCTGCCCCTGCTGGCCGACAGCACCGACGGCATGGCCTCCGTGGCCGCATAC
ACCTACCGGCAGCACTCTGTGGTCTTCATTGGCACGCGCAGCGGCAGCTTGAAGAAGGTG
CGGGTCGATGGCTTCCAGGATGCCCACCTGTATGAGACAGTGCCAGTGGTGGATGGCTCT
CCCATCCTCCGAGACTTGCTGTTCAGTCCGGACCACCGGCATATCTATCTCCTGAGTGAG
AAACAGGTGAGCCAGCTCCCGGTGGAGACATGTGAGCAGTACCAGAGCTGCGCAGCCTGC
CTGGGCTCCGGGGACCCGCACTGTGGTTGGTGTGTGCTCCGACACAGGTGCTGCCGCGAA
GGAGCCTGTCTGGGCGCCTCTGCCCCACATGGCTTTGCTGAGGAGCTGAGCAAGTGTGTC
CAGGTGAGGGTACGGCCCAACAATGTCTCAGTGACGTCACCTGGGGTGCAGCTCACCGTC
ACACTGCACAACGTGCCAGACCTCAGTGCGGGCGTGAGCTGCGCCTTCGAGGCGGCGGCG
GAGAACGAGGCGGTCCTGCTGCCCTCCGGTGAACTGCTGTGCCCCTCACCCTCCCTCCAG
GAGCTCCGAGCTCTTACCAGGGGGCACGGAGCCACCCGCACTGTGCGGCTGCAGCTGCTC
TCCAAGGAGACAGGCGTGAGGTTTGCCGGTGCTGACTTTGTCTTCTACAACTGCAGCGTC
CTCCAGTCATGCATGTCTTGTGTTGGCAGCCCTTACCCCTGCCACTGGTGTAAGTACCGC
CACACGTGTACCAGCCGCCCCCACGAGTGCTCCTTCCAGGAGGGCAGGGTCCACAGCCCC
GAGGGCTGCCCTGAGATCCTGCCCAGTGGGGACCTTCTGATCCCCGTTGGGGTCATGCAG
CCTCTTACCCTGCGGGCTAAGAACCTACCTCAGCCGCAGTCGGGCCAGAAAAACTATGAG
TGCGTGGTGCGGGTGCAGGGGCGGCAGCAGCGGGTGCCTGCCGTGCGCTTCAACAGTAGC
AGTGTGCAGTGCCAAAACGCCTCGTACTCCTATGAAGGCGATGAGCATGGTGACACCGAG
CTGGATTTTTCCGTGGTCTGGGATGGAGACTTCCCCATAGACAAGCCTCCTAGCTTCCGA
GCACTCCTCTACAAGTGCTGGGCGCAACGGCCCAGCTGTGGCCTCTGCCTCAAGGCTGAT
CCCCGCTTCAACTGTGGCTGGTGCATCTCAGAGCACAGGTGCCAGCTGCGGACCCACTGC
CCGGCCCCGAAGACCAACTGGATGCACCTGAGCCAGAAGGGCACCCGGTGCAGCCACCCC
CGCATCACGCAGATCCATCCTCTCGTGGGGCCCAAGGAAGGAGGCACCCGGGTCACCATC
GTGGGTGAGAACCTGGGCCTCTTGTCCCGAGAGGTGGGCCTGCGGGTGGCTGGCGTGCGT
TGTAACTCCATTCCGGCCGAGTACATTAGTGCAGAGAGGATCGTGTGTGAGATGGAGGAG
TCGCTGGTGCCCAGCCCCCCACCGGGGCCCGTGGAGCTGTGCGTGGGTGACTGTTCAGCC
GACTTCCGGACGCAGTCGGAGCAGGTCTACAGCTTTGTGACCCCAACGTTTGACCAGGTG
AGTCCCAGCCGTGGCCCGGCGTCCGGGGGCACACGGCTTACCATCTCCGGCAGCTCTCTG
GATGCTGGCAGCAGGGTCACAGTGACTGTGAGGGACAGCGAGTGCCAGTTTGTACGGAGG
GATGCTAAGGCGATCGTCTGCATCTCACCTCTCTCCACCTTGGGCCCCAGCCAGGCCCCC
ATCACACTTGCTATTGACCGGGCTAACATCTCCTCCCCTGGCCTCATCTACACCTACACT
CAGGACCCCACAGTCACCCGCCTGGAGCCCACCTGGAGCATCATCAATGGATCCACTGCC
ATCACTGTCAGTGGGACCCATCTGCTGACGGTCCAGGAACCCCGGGTCCGTGCCAAGTAC
CGCGGCATTGAGACAACCAATACATGCCAAGTGATCAACGACACCGCCATGCTGTGTAAG
GCACCCGGCATCTTTCTTGGGCGGCCACAGCCCCGGGCGCAAGGCGAGCACCCTGATGAG
TTTGGCTTCCTTCTGGACCACGTGCAAACGGCCCGCTCCCTCAACCGCTCCTCCTTTACC
TACTACCCTGATCCCAGCTTTGAGCCGCTGGGGCCCTCAGGCGTGCTGGACGTCAAACCG
GGCTCCCACGTGGTGCTGAAGGGCAAGAACCTGATTCCCGCTGCAGCCGGCAGCTCCCGC
CTCAACTACACTGTGCTGATAGGAGGCCAGCCGTGTTCGCTCACAGTCTCGGACACACAA
CTCCTGTGCGACTCACCTAGTCAGACTGGCCGGCAGCCTGTCATGGTGCTGGTTGGTGGA
CTGGAGTTCTGGCTGGGCACCCTGCACATCTCGGCAGAGCGGGCGCTGACCCTACCGGCC
ATGATGGGGCTGGCGGCCGGGGGTGGGCTCCTGCTGCTGGCCATCACAGCCGTGCTGGTA
GCTTACAAGCGCAAGACTCAGGACGCGGACCGTACACTTAAGAGACTGCAGCTGCAGATG
GACAACCTGGAGTCCCGTGTGGCCCTGGAGTGCAAGGAAGCATTTGCAGAGCTGCAGACG
GACATCAATGAGTTGACTAACCACATGGACGAGGTGCAGATCCCCTTCCTGGACTACCGG
ACTTACGCCGTGCGCGTGCTCTTCCCGGGCATAGAGGCCCATCCGGTGCTCAAGGAGCTG
GATACGCCACCCAACGTGGAGAAGGCCCTGCGCCTCTTCGGGCAGCTCCTGCACAGCCGC
GCGTTCGTGCTTACCTTCATCCACACGCTCGAGGCCCAGTCAAGCTTCTCCATGCGCGAC
AGAGGCACCGTGGCCTCGCTCACCATGGTCGCCCTGCAGAGCCGGCTCGACTATGCCACC
GGCCTGCTGAAGCAACTTCTGGCCGACCTCATCGAGAAGAACCTCGAGAGTAAGAACCAC
CCCAAGCTGCTGCTACGCAGGACAGAGTCAGTTGCTGAGAAGATGCTTACCAACTGGTTC
ACGTTCCTGCTGCATAAGTTTCTGAAGGAGTGTGCTGGCGAGCCTCTCTTCCTGCTTTAC
TGTGCCATCAAGCAGCAGATGGAGAAGGGCCCCATTGATGCCATCACGGGCGAGGCACGA
TACTCCCTGAGCGAGGACAAGCTCATCCGTCAGCAGATCGACTACAAGACACTGACCCTT
CACTGTGTGTGTCCGGAGAACGAGGGCAGCGCCCAGGTCCCAGTGAAGGTTCTCAACTGT
GACAGTATCACACAGGCCAAAGATAAGCTGCTGGACACTGTGTACAAGGGCATTCCGTAC
TCCCAGCGTCCCAAAGCTGAGGACATGGACCTGGAGTGGCGCCAGGGCCGCATGACTCGC
ATCATCCTCCAGGATGAGGATGTCACCACCAAGATCGAGTGTGACTGGAAGAGGCTCAAC
TCACTGGCCCACTACCAGGTGACAGACGGTTCCTTGGTGGCATTGGTGCCCAAACAAGTG
TCTGCCTATAACATGGCCAACTCCTTCACCTTCACCCGCTCCCTCAGTCGCTACGAGAGC
TTGCTCCGCACGGCCAGCAGCCCTGATTCTCTCCGCTCACGGGCACCCATGATTACGCCT
GACCAGGAAACAGGCACAAAATTGTGGCACCTGGTGAAAAACCACGACCATGCCGACCAT
CGCGAGGGGGACCGTGGCAGTAAGATGGTCAGCGAGATCTACCTGACTCGGCTCCTGGCC
ACAAAGGGCACACTGCAGAAGTTTGTGGATGACCTCTTTGAGACAGTGTTCAGCACAGCC
CACCGGGGCTCGGCCCTGCCCCTCGCCATCAAGTACATGTTCGACTTCCTGGATGAACAG
GCGGACCAGCGCCAGATCAGCGACCCCGATGTGCGCCACACCTGGAAGAGCAACTGCCTC
CCGCTGCGATTCTGGGTGAATGTGATCAAGAACCCGCAGTTCGTGTTCGACATCCACAAG
AACAGCATCACGGATGCCTGCCTGTCGGTGGTAGCCCAGACCTTCATGGACTCCTGCTCT
ACCTCCGAGCACCGCCTCGGGAAGGACTCGCCTTCCAACAAACTTCTCTACGCCAAGGAC
ATCCCCAACTACAAGAGCTGGGTGGAGAGGTATTATCGAGACATTGCAAAGATGGCATCC
ATCAGCGACCAGGACATGGATGCCTACCTGGTGGAGCAGTCCCGCCTCCACGCGAGCGAC
TTCTCTGTCCTCAGCGCGCTCAACGAGCTGTATTTCTATGTCACAAAGTACCGCCAGGAG
ATTCTCACGGCTCTGGACCGCGACGCCTCTTGTCGGAAGCATAAGTTGCGGCAGAAACTG
GAACAGATCATCAGCCTCGTGTCCAGCGACAGC
Protein sequence predicted based on nucleotide sequence
MPSVCLLLLLFLAVGGALGNRPFRAFVVTDTTLTHLAVHRVTGEVFVGAVNRVFKLAPNL TELRAHVTGPVEDNARCYPPPSMRVCAHRLAPVDNINKLLLIDYAARRLVACGSIWQGIC QFLRLDDLFKLGEPHHRKEHYLSGAQEPDSMAGVIVEQGQGPSKLFVGTAVDGKSEYFPT LSSRKLISDEDSADMFSLVYQDEFVSSQIKIPSDTLSLYPAFDIYYIYGFVSASFVYFLT LQLDTQQTLLDTAGEKFFTSKIVRMCAGDSEFYSYVEFPIGCSWRGVEYRLVQSAHLAKP GLLLAQALGVPADEDVLFTIFSQGQKNRASPPRQTILCLFTLSNINAHIRRRIQSCYRGE GTLALPWLLNKELPCINTPMQINGNFCGLVLNQPLGGLHVIEGLPLLADSTDGMASVAAY TYRQHSVVFIGTRSGSLKKVRVDGFQDAHLYETVPVVDGSPILRDLLFSPDHRHIYLLSE KQVSQLPVETCEQYQSCAACLGSGDPHCGWCVLRHRCCREGACLGASAPHGFAEELSKCV QVRVRPNNVSVTSPGVQLTVTLHNVPDLSAGVSCAFEAAAENEAVLLPSGELLCPSPSLQ ELRALTRGHGATRTVRLQLLSKETGVRFAGADFVFYNCSVLQSCMSCVGSPYPCHWCKYR HTCTSRPHECSFQEGRVHSPEGCPEILPSGDLLIPVGVMQPLTLRAKNLPQPQSGQKNYE CVVRVQGRQQRVPAVRFNSSSVQCQNASYSYEGDEHGDTELDFSVVWDGDFPIDKPPSFR ALLYKCWAQRPSCGLCLKADPRFNCGWCISEHRCQLRTHCPAPKTNWMHLSQKGTRCSHP RITQIHPLVGPKEGGTRVTIVGENLGLLSREVGLRVAGVRCNSIPAEYISAERIVCEMEE SLVPSPPPGPVELCVGDCSADFRTQSEQVYSFVTPTFDQVSPSRGPASGGTRLTISGSSL DAGSRVTVTVRDSECQFVRRDAKAIVCISPLSTLGPSQAPITLAIDRANISSPGLIYTYT QDPTVTRLEPTWSIINGSTAITVSGTHLLTVQEPRVRAKYRGIETTNTCQVINDTAMLCK APGIFLGRPQPRAQGEHPDEFGFLLDHVQTARSLNRSSFTYYPDPSFEPLGPSGVLDVKP GSHVVLKGKNLIPAAAGSSRLNYTVLIGGQPCSLTVSDTQLLCDSPSQTGRQPVMVLVGG LEFWLGTLHISAERALTLPAMMGLAAGGGLLLLAITAVLVAYKRKTQDADRTLKRLQLQM DNLESRVALECKEAFAELQTDINELTNHMDEVQIPFLDYRTYAVRVLFPGIEAHPVLKEL DTPPNVEKALRLFGQLLHSRAFVLTFIHTLEAQSSFSMRDRGTVASLTMVALQSRLDYAT GLLKQLLADLIEKNLESKNHPKLLLRRTESVAEKMLTNWFTFLLHKFLKECAGEPLFLLY CAIKQQMEKGPIDAITGEARYSLSEDKLIRQQIDYKTLTLHCVCPENEGSAQVPVKVLNC DSITQAKDKLLDTVYKGIPYSQRPKAEDMDLEWRQGRMTRIILQDEDVTTKIECDWKRLN SLAHYQVTDGSLVALVPKQVSAYNMANSFTFTRSLSRYESLLRTASSPDSLRSRAPMITP DQETGTKLWHLVKNHDHADHREGDRGSKMVSEIYLTRLLATKGTLQKFVDDLFETVFSTA HRGSALPLAIKYMFDFLDEQADQRQISDPDVRHTWKSNCLPLRFWVNVIKNPQFVFDIHK NSITDACLSVVAQTFMDSCSTSEHRLGKDSPSNKLLYAKDIPNYKSWVERYYRDIAKMAS ISDQDMDAYLVEQSRLHASDFSVLSALNELYFYVTKYRQEILTALDRDASCRKHKLRQKL EQIISLVSSDS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
