Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
ORFeome_LaBaer-010.0192
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00822129
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3486nts
Open reading frame: 1 to 3486
ATGGAAGAAGTTAGGTGTCCAGAGCACGGGACTTTCTGCTTTCTTAAGACCGGCGTCCGC
GATGGCCCGAATAAAGGAAAGAGCTTCTACGTGTGCCGGGCAGACACGTGCAGCTTCGTG
CGGGCCACCGACATTCCTGTTTCCCATTGCTTATTGCATGAGGACTTTGTGGTAGAGCTT
CAGGGTTTGCTTCTGCCACAGGACAAGAAAGAATACAGATTGTTCTTCCGATGCATTAGA
AGTAAGGCAGAGGGGAAACGCTGGTGTGGAAGTATTCCATGGCAGGATCCTGATTCCAAA
GAACATTCTGTATCCAATAAGTCTCAGCATGCATCTGAGACATTTCATCATTCTTCCAAC
TGGCTGAGAAATCCATTCAAGGTACTTGACAAGAATCAAGAACCAGCTCTCTGGAAACAG
CTCATCAAAGGTGAAGGTGAGGAAAAGAAGGCTGATAAGAAGCAAAGAGAAAAGGGAGAT
CAGCTTTTCGATCAAAAGAAAGAACAGAAGCCTGAAATGATGGAGAAAGACCTCTCATCT
GGCCTGGTACCAAAGAAAAAACAATCTGTAGTTCAAGAGAAGAAGCAAGAAGAGGGAGCA
GAGATTCAGTGTGAGGCAGAGACTGGAGGCACACACAAAAGAGACTTTTCTGAAATTAAA
TCTCAACAGTGCCAAGGTAATGAGCTTACAAGACCATCTGCATCTTCTCAGGAGAAATCA
AGTGGTAAGAGTCAAGATGTCCAAAGAGAATCAGAACCTCTGAGAGAAAAGGTTACCCAG
CTTTTGCCTCAAAATGTTCACAGTCACAACTCAATAAGCAAGCCCCAGAAAGGGGGACCC
CTCAACAAGGAGTACACGAACTGGGAGGCTAAAGAAACAAAGGCAAAGGATGGCCCTAGC
ATACAGGCCACCCAGAAAAGCCTGCCTCAGGGGCATTTCCAAGAGCGGCCGGAAACCCAC
AGTGTGCCTGCTCCTGGAGGACCAGCGGCTCAGGCTGCACCAGCAGCACCAGGGCTTTCC
CTGGGTGAGGGCCGTGAAGCTGCCACAAGCAGTGACGACGAGGAGGAAGATGATGTTGTT
TTTGTTTCCTCTAAGCCTGGGAGCCCCCTACTCTTTGACTCGACTCTGGACTTAGAGACG
AAGGAAAACCTCCAATTCCCTGATCGAAGTGTGCAAAGAAAGGTGTCTCCTGCCTCAGGT
GTTTCCAAGAAGGTAGAACCCTCAGACCCAGTAGCCCGGCGTGTCTACCTTACAACACAA
CTGAAACAAAAGAAGAGCACACTGGCATCAGTGAACATCCAGGCTCTTCCAGACAAGGGT
CAGAAGTTGATCAAACAAATCCAGGAGCTGGAGGAAGTACTCAGTGGTCTTACCCTTTCC
CCAGAGCAAGGAACTAATGAGAAGAGTAACAGTCAAGTACCACAGCAGAGTCACTTCACC
AAAACTACCACTGGCCCTCCCCACCTGGTGCCTCCCCAACCCCTTCCTCGTCGTGGTACC
CAACCTGTGGGTTCTCTAGAACTAAAGTCTGCCTGCCAGGTGACTGCTGGAGGATCCAGT
CAGTGCTATCGAGGCCATACAAACCAAGATCACGTTCATGCAGTGTGGAAAATCACAAGT
GAAGCCATCGGTCAACTGCATCGCTCACTTGAGTCATGTCCTGGTGAGACGGTTGTGGCA
GAAGATCCCGCCGGGCTGAAGGTCCCTTTGCTACTACACCAGAAGCAGGCATTGGCTTGG
TTACTATGGCGAGAAAGTCAGAAGCCACAAGGAGGAATTCTGGCAGATGATATGGGCTTA
GGAAAAACCCTGACAATGATTGCGCTCATCCTGACCCAGAAGAATCAAGAGAAAAAGGAA
GAAAAGGAGAAAAGCACAGCTTTGACGTGGCTCTCCAAAGATGACTCTTGTGACTTTACT
TCCCATGGAACACTAATCATCTGTCCTGCCTCCCTGATCCATCATTGGAAAAATGAGGTG
GAGAAACGGGTGAACAGCAACAAACTAAGAGTCTATCTCTACCATGGGCCAAACCGGGAT
TCACGTGCCAGAGTCCTCTCTACATATGACATCGTGATCACTACCTATAGCCTCGTGGCC
AAGGAGATTCCCACAAACAAGCAAGAGGCAGAGATCCCAGGTGCAAACCTCAATGTGGAG
GGCACCTCAACACCTTTGCTTCGAATAGCCTGGGCTCGAATCATATTGGATGAAGCTCAC
AATGTTAAGAATCCCCGAGTGCAGACTTCCATAGCTGTGTGTAAGCTACAAGCCTGTGCC
CGTTGGGCTGTCACTGGAACCCCCATTCAAAACAACTTATTGGATATGTATTCGCTGCTG
AAGTTTCTCCGTTGCTCTCCATTTGATGAGTTCAATCTGTGGAGGAGTCAGGTTGACAAT
GGCTCAAAGAAAGGAGGAGAACGGTTAAGTATTTTAACCAAGAGCCTTTTGCTGAGGAGA
ACAAAAGACCAGCTGGACTCTACTGGCAGACCTTTGGTGATACTGCCCCAGCGTAAATTT
CAGTTGCACCATTTAAAGCTTTCTGAAGATGAAGAGACTGTTTACAATGTGTTTTTTGCA
AGATCAAGGTCAGCTCTGCAATCCTATCTAAAAAGACATGAAAGTAGAGGCAACCAATCT
GGAAGAAGCCCTAATAATCCATTCAGTAGAGTGGCACTGGAGTTTGGGTCTGAGGAGCCT
AGACACTCGGAGGCAGCAGACTCACCGAGATCCAGCACCGTCCACATACTGTCCCAGTTG
CTGAGACTCCGCCAGTGTTGCTGTCATCTTTCTTTACTGAAGTCGGCCCTGGATCCAATG
GAACTGAAGGGTGAAGGTCTTGTCCTTTCCCTGGAAGAACAGCTCAGTGCTTTGACCTTG
TCAGAACTCCGTGACTCAGAGCCATCTTCCACTGTTTCCCTTAACGGCACCTTCTTCAAG
ATGGAGCTTTTTGAAGGCATGCGAGAGAGCACCAAGATTTCATCTCTGTTGGCAGAATTG
GAGGCAATTCAAAGAAATTCAGCATCCCAAAAGAGTGTCATTGTCTCTCAGTGGACCAAC
ATGCTGAAAGTTGTAGCATTGCACCTGAAGAAGCATGGACTGACTTATGCCACCATCGAT
GGCTCTGTCAATCCCAAGCAGAGAATGGACTTGGTAGAGGCATTTAACCACTCCAGAGGC
CCTCAGGTAATGCTAATCTCTCTCTTGGCCGGAGGTGTTGGTCTAAACCTGACTGGAGGA
AATCACCTCTTTCTTTTGGACATGCACTGGAATCCATCACTTGAAGATCAAGCTTGTGAC
CGAATTTACCGAGTAGGGCAGCAGAAAGATGTTGTCATACACAGATTTGTTTGTGAGGGA
ACAGTAGAAGAAAAGATCTTACAGCTCCAAGAAAAAAAGAAAGATTTGGCCAAACAAGTT
CTATCAGGGTCTGGAGAATCTGTCACCAAGCTCACCTTGGCTGACCTCAGAGTCCTTTTT
GGCATC
Protein sequence predicted based on nucleotide sequence
MEEVRCPEHGTFCFLKTGVRDGPNKGKSFYVCRADTCSFVRATDIPVSHCLLHEDFVVEL QGLLLPQDKKEYRLFFRCIRSKAEGKRWCGSIPWQDPDSKEHSVSNKSQHASETFHHSSN WLRNPFKVLDKNQEPALWKQLIKGEGEEKKADKKQREKGDQLFDQKKEQKPEMMEKDLSS GLVPKKKQSVVQEKKQEEGAEIQCEAETGGTHKRDFSEIKSQQCQGNELTRPSASSQEKS SGKSQDVQRESEPLREKVTQLLPQNVHSHNSISKPQKGGPLNKEYTNWEAKETKAKDGPS IQATQKSLPQGHFQERPETHSVPAPGGPAAQAAPAAPGLSLGEGREAATSSDDEEEDDVV FVSSKPGSPLLFDSTLDLETKENLQFPDRSVQRKVSPASGVSKKVEPSDPVARRVYLTTQ LKQKKSTLASVNIQALPDKGQKLIKQIQELEEVLSGLTLSPEQGTNEKSNSQVPQQSHFT KTTTGPPHLVPPQPLPRRGTQPVGSLELKSACQVTAGGSSQCYRGHTNQDHVHAVWKITS EAIGQLHRSLESCPGETVVAEDPAGLKVPLLLHQKQALAWLLWRESQKPQGGILADDMGL GKTLTMIALILTQKNQEKKEEKEKSTALTWLSKDDSCDFTSHGTLIICPASLIHHWKNEV EKRVNSNKLRVYLYHGPNRDSRARVLSTYDIVITTYSLVAKEIPTNKQEAEIPGANLNVE GTSTPLLRIAWARIILDEAHNVKNPRVQTSIAVCKLQACARWAVTGTPIQNNLLDMYSLL KFLRCSPFDEFNLWRSQVDNGSKKGGERLSILTKSLLLRRTKDQLDSTGRPLVILPQRKF QLHHLKLSEDEETVYNVFFARSRSALQSYLKRHESRGNQSGRSPNNPFSRVALEFGSEEP RHSEAADSPRSSTVHILSQLLRLRQCCCHLSLLKSALDPMELKGEGLVLSLEEQLSALTL SELRDSEPSSTVSLNGTFFKMELFEGMRESTKISSLLAELEAIQRNSASQKSVIVSQWTN MLKVVALHLKKHGLTYATIDGSVNPKQRMDLVEAFNHSRGPQVMLISLLAGGVGLNLTGG NHLFLLDMHWNPSLEDQACDRIYRVGQQKDVVIHRFVCEGTVEEKILQLQEKKKDLAKQV LSGSGESVTKLTLADLRVLFGI
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
