Gene:
Gene Symbol:
BNC1
Gene Name:
basonuclin 1
Original Clone ID:
FLH267371.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00082594
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCGGCGGCGCCCGCCGAGCCGGGGCGGACGCGGGGCGGCCCGGGCCCGGGAGACGCGC
CGGCAGCCCCGGCACCGCAGCGGTCGCAGGATGGCCGAGGCTATCAGCTGTACTCTGAAC
TGTAGTTGCCAAAGTTTCAAACCCGGGAAAATAAACCACCGTCAGTGTGACCAATGCAAG
CATGGATGGGTGGCCCACGCTCTAAGTAAGCTAAGGATCCCCCCCATGTATCCAACAAGC
CAGGTGGAGATTGTCCAGTCCAATGTAGTGTTTGATATTAGCAGCCTCATGCTCTATGGG
ACCCAGGCCATCCCCGTTCGCCTAAAAATCCTACTGGACCGGCTCTTCAGTGTGTTGAAG
CAAGATGAGGTTCTCCAGATCCTCCATGCCTTGGACTGGACACTTCAGGATTATATCCGT
GGATACGTACTGCAGGATGCATCAGGAAAGGTGTTGGATCACTGGAGCATCATGACCAGT
GAGGAAGAAGTGGCCACCTTGCAGCAGTTCCTTCGTTTTGGAGAGACCAAATCTATAGTT
GAACTCATGGCAATTCAAGAGAAAGAAGAGCAATCCATCATCATACCACCTTCCACAGCA
AATGTAGATATCAGGGCTTTCATCGAGAGCTGCAGTCACAGGAGTTCTAGCCTCCCCACT
CCTGTGGACAAAGGAAACCCCAGCAGTATACACCCCTTTGAGAACCTCATAAGCAACATG
ACTTTCATGCTGCCTTTCCAGTTCTTCAACCCTCTGCCTCCTGCACTGATAGGGTCATTG
CCCGAACAATATATGTTGGAGCAGGGTCATGACCAAAGTCAGGACCCCAAACAGGAAGTC
CATGGGCCCTTCCCTGACAGCAGCTTCTTAACTTCCAGTTCCACACCATTTCAGGTTGAA
AAAGATCAGTGTTTAAACTGTCCGGATGCTATTACTAAAAAAGAAGACAGCACCCATTTA
AGTGACTCCAGCTCATACAACATTGTCACTAAGTTTGAAAGGACACAGTTATCCCCTGAG
GCCAAAGTGAAGCCTGAGAGGAATAGCCTTGGTACAAAGAAGGGCCGGGTGTTCTGCACT
GCATGTGAGAAGACCTTCTATGACAAAGGCACCCTCAAAATCCACTACAATGCCGTCCAC
TTGAAGATCAAGCATAAGTGCACCATCGAAGGGTGTAACATGGTGTTCAGCTCCCTAAGG
AGCCGGAATCGCCATAGCGCCAACCCCAACCCTCGGCTGCACATGCCAATGAACAGAAAT
AACCGGGACAAAGACCTCAGGAACAGCCTGAACCTGGCCAGCTCTGAGAACTACAAGTGC
CCAGGTTTCACAGTGACGTCCCCAGACTGTAGGCCTCCTCCCAGCTACCCTGGTTCAGGA
GAGGATTCCAAAGGCCAACCAGCCTTCCCAAACATTGGGCAAAATGGTGTGCTTTTTCCC
AACCTAAAGACAGTCCAGCCAGTCCTTCCTTTCTACCGCAGTCCAGCCACGCCTGCCGAG
GTAGCAAACACGCCTGGGATACTCCCTTCCCTCCCGCTGTTGTCCTCTTCAATCCCAGAA
CAGCTCATTTCAAACGAAATGCCATTTGATGCCCTTCCCAAGAAGAAATCCAGGAAGTCC
AGTATGCCTATCAAAATAGAGAAAGAAGCTGTGGAAATAGCTAATGAGAAAAGACACAAC
CTCAGCTCAGATGAAGACATGCCCCTACAGGTGGTCAGTGAAGATGAGCAGGAGGCCTGC
AGTCCTCAGTCACACAGAGTATCTGAGGAGCAGCATGTACAGTCAGGAGGCTTAGGGAAG
CCTTTCCCTGAAGGGGAGAGGCCCTGCCATCGTGAATCAGTAATTGAGTCCAGTGGAGCC
ATCAGCCAAACCCCTGAGCAGGCCACACACAATTCAGAGAGGGAGACTGAGCAGACACCA
GCATTGATCATGGTGCCAAGGGAGGTCGAGGATGGTGGCCATGAACACTACTTCACACCT
GGGATGGAACCCCAAGTTCCTTTTTCTGACTACATGGAACTGCAGCAGCGCCTGCTGGCT
GGGGGACTCTTCAGTGCTTTGTCCAACAGGGGAATGGCTTTTCCTTGTCTTGAAGATTCT
AAAGAACTGGAGCACGTGGGTCAGCATGCATTAGCAAGGCAGATAGAAGAAAATCGCTTC
CAGTGTGACATCTGCAAGAAGACCTTTAAAAATGCTTGTAGTGTGAAAATTCATCACAAG
AATATGCATGTCAAAGAAATGCACACATGCACAGTGGAGGGCTGTAATGCTACCTTTCCC
TCCCGCAGGAGCAGAGACAGACACAGCTCAAACCTAAACCTCCACCAAAAAGCATTGAGC
CAGGAAGCATTGGAGAGTAGTGAAGATCATTTCCGTGCAGCTTACCTTCTGAAAGATGTG
GCTAAGGAAGCCTATCAGGATGTGGCTTTTACACAGCAAGCCTCCCAGACATCTGTCATC
TTCAAAGGAACAAGTCGAATGGGCAGTCTGGTTTACCCAATAACGCAAGTCCACAGTGCC
AGCCTGGAGAGCTACAACTCTGGCCCCTTGAGCGAGGGCACCATCCTGGATTTGAGCACT
ACCTCGAGCATGAAGTCAGAGAGTAGCAGCCATTCTTCCTGGGACTCTGACGGGGTGAGT
GAGGAAGGCACTGTGCTTATGGAGGACAGTGATGGGAACTGTGAAGGGTCGAGCCTTGTC
CCTGGGGAAGATGAGTACCCCATCTGTGTCCTGATGGAGAAGGCTGACCAGAGCCTTGCT
AGCCTGCCTTCTGGGTTGCCCATAACCTGTCATCTCTGCCAAAAGACATACAGTAACAAA
GGGACCTTTAGGGCCCACTACAAAACTGTGCACCTCCGGCAGCTCCACAAATGCAAAGTA
CCAGGCTGCAACACCATGTTTTCGTCTGTTCGCAGTCGAAACAGACACAGCCAGAATCCC
AACCTGCACAAAAGCCTGGCCTCATCTCCAAGTCACCTCCAG
Protein sequence predicted based on nucleotide sequence
MRRRPPSRGGRGAARARETRRQPRHRSGRRMAEAISCTLNCSCQSFKPGKINHRQCDQCK HGWVAHALSKLRIPPMYPTSQVEIVQSNVVFDISSLMLYGTQAIPVRLKILLDRLFSVLK QDEVLQILHALDWTLQDYIRGYVLQDASGKVLDHWSIMTSEEEVATLQQFLRFGETKSIV ELMAIQEKEEQSIIIPPSTANVDIRAFIESCSHRSSSLPTPVDKGNPSSIHPFENLISNM TFMLPFQFFNPLPPALIGSLPEQYMLEQGHDQSQDPKQEVHGPFPDSSFLTSSSTPFQVE KDQCLNCPDAITKKEDSTHLSDSSSYNIVTKFERTQLSPEAKVKPERNSLGTKKGRVFCT ACEKTFYDKGTLKIHYNAVHLKIKHKCTIEGCNMVFSSLRSRNRHSANPNPRLHMPMNRN NRDKDLRNSLNLASSENYKCPGFTVTSPDCRPPPSYPGSGEDSKGQPAFPNIGQNGVLFP NLKTVQPVLPFYRSPATPAEVANTPGILPSLPLLSSSIPEQLISNEMPFDALPKKKSRKS SMPIKIEKEAVEIANEKRHNLSSDEDMPLQVVSEDEQEACSPQSHRVSEEQHVQSGGLGK PFPEGERPCHRESVIESSGAISQTPEQATHNSERETEQTPALIMVPREVEDGGHEHYFTP GMEPQVPFSDYMELQQRLLAGGLFSALSNRGMAFPCLEDSKELEHVGQHALARQIEENRF QCDICKKTFKNACSVKIHHKNMHVKEMHTCTVEGCNATFPSRRSRDRHSSNLNLHQKALS QEALESSEDHFRAAYLLKDVAKEAYQDVAFTQQASQTSVIFKGTSRMGSLVYPITQVHSA SLESYNSGPLSEGTILDLSTTSSMKSESSSHSSWDSDGVSEEGTVLMEDSDGNCEGSSLV PGEDEYPICVLMEKADQSLASLPSGLPITCHLCQKTYSNKGTFRAHYKTVHLRQLHKCKV PGCNTMFSSVRSRNRHSQNPNLHKSLASSPSHLQ
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3019
Start on reference sequence
35
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
