PPP1R3A (H. sapiens ) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  PPP1R3A
Gene Name:  protein phosphatase 1, regulatory subunit 3A
Original Clone ID:
FLH267396.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00082619
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3366nts
Open reading frame: 1 to 3366

ATGGAGCCTTCTGAAGTACCTAGTCAGATTAGCAAAGATAATTTTTTAGAAGTTCCTAAT
TTATCTGACTCTCTTTGTGAAGATGAAGAAGTTACTTTCCAACCTGGTTTCTCCCCTCAA
CCAAGTAGACGAGGTTCTGATTCTTCTGAAGACATATACCTGGATACCCCATCTTCAGGT
ACTAGAAGAGTTTCATTTGCTGATTCCTTTGGATTCAATCTTGTGTCTGTTAAAGAATTT
GATTGCTGGGAATTACCGAGTGCTTCAACCACTTTTGACTTAGGGACGGACATTTTCCAC
ACAGAAGAATATGTTTTAGCCCCACTGTTTGACTTGCCTTCTTCAAAAGAAGATCTTATG
CAACAACTCCAAATACAGAAAGCAATACTGGAGTCAACTGAGTCTCTTCTTGGGTCTACA
AGTATCAAGGGTATTATTCGAGTTTTGAATGTTTCTTTTGAGAAGTTAGTATATGTAAGA
ATGTCTTTAGATGACTGGCAGACACATTATGACATTTTAGCAGAATATGTTCCTAATTCA
TGTGATGGTGAAACTGACCAGTTCTCCTTTAAGATTGTATTGGTTCCTCCTTATCAAAAA
GATGGCAGTAAAGTTGAGTTTTGTATACGTTATGAAACTTCTGTTGGTACATTTTGGTCA
AATAATAATGGCACAAATTATACATTCATTTGTCAAAAGAAAGAACAAGAGCCGGAGCCT
GTAAAACCATGGAAAGAAGTTCCTAACAGACAAATAAAAGGCTGCTTAAAGGTAAAATCA
AGTAAAGAAGAATCATCAGTAACATCAGAAGAAAATAACTTTGAGAATCCAAAGAATACA
GATACCTATATCCCAACAATCATTTGTTCTCATGAGGACAAGGAAGATTTGGAAGCCAGT
AATCGAAATGTAAAAGATGTAAACAGGGAACATGATGAACATAATGAAAAAGAATTAGAG
TTGATGATAAATCAACACTTAATAAGAACCAGAAGTACTGCTTCCAGAGATGAAAGGAAT
ACATTTTCAACAGATCCAGTCAATTTTCCAAATAAAGCAGAGGGGTTAGAGAAGAAGCAA
ATCCATGGTGAAATATGTACTGACTTGTTCCAAAGGTCTCTGTCTCCAAGTTCATCAGCA
GAAAGCTCCGTAAAGGGAGATTTTTACTGCAATGAAAAATATTCCTCAGGAGATGACTGT
ACACATCAACCTTCAGAGGAAACTACTTCAAATATGGGAGAAATCAAGCCATCATTGGGA
GATACTAGTAGTGATGAACTAGTGCAATTACATACTGGCAGCAAAGAAGTCCTGGATGAT
AATGCTAATCCAGCCCATGGCAATGGCACAGTGCAAATACCTTGCCCCTCTTCAGATCAA
CTAATGGCAGGAAACCTTAATAAAAAACATGAAGGAGGAGCTAAAAATATTGAAGTAAAA
GATTTGGGATGTTTACGAAGAGATTTCCATTCAGATACGTCGGCATGTCTCAAAGAATCA
ACAGAAGAAGGATCTTCTAAGGAAGATTATTATGGCAATGGTAAGGATGATGAAGAACAA
AGAATATATTTAGGTGTTAATGAAAAACAAAGAAAAAATTTCCAAACAATCTTACATGAC
CAAGAAAGGAAGATGGGTAACCCTAAAATAAGTGTGGCAGGGATTGGAGCTAGTAACAGA
GACCTGGCTACTCTGCTGAGCGAACATACCGCAATCCCCACCCGGGCAATCACAGCAGAT
GTGTCTCATTCACCAAGGACAAATTTAAGTTGGGAAGAAGCTGTGTTAACCCCAGAGCAT
CATCATTTGACTAGTGAAGGCAGCGCTTTAGGAGGGATAACTGGTCAAGTTTGTTCATCA
AGAACTGGAAATGTTTTGAGGAATGATTATCTTTTCCAAGTTGAAGAAAAATCAGGTGGG
ATTAATTCTGAAGATCAGGATAATAGCCCACAGCATAAACAAAGTTGGAATGTTCTGGAA
AGTCAGGGAAAATCAAGAGAGAATAAGACAAACATAACAGAGCATATCAAAGGACAAACA
GATTGTGAAGACGTGTGGGGAAAAAGAGATAATACGAGGAGTTTGAAAGCTACTACAGAA
GAATTGTTTACCTGCCAAGAAACAGTGTGCTGTGAACTGTCTTCTCTAGCTGATCATGGC
ATTACTGAGAAAGCAGAAGCTGGTACAGCCTATATAATTAAGACAACATCAGAAAGTACT
CCAGAAAGCATGTCTGCTAGAGAAAAAGCAATAATTGCTAAGCTACCTCAAGAGACAGCA
CGAAGTGACAGGCCCATCGAGGTAAAGGAAACAGCGTTTGATCCACATGAAGGGAGAAAT
GATGATTCACATTATACCCTTTGTCAACGAGATACAGTAGGTGTAATCTATGACAATGAT
TTTGAAAAGGAATCACGTTTAGGTATTTGTAATGTACGTGTAGATGAAATGGAGAAGGAA
GAAACCATGTCTATGTACAATCCTAGGAAGACACATGACAGGGAGAAATGTGGCACTGGA
AATATAACATCTGTGGAAGAATCCTCATGGGTCATTACAGAATATCAAAAAGCAACTTCA
AAACTGGATTTACAGTTGGGAATGTTACCAACAGACAAAACTGTATTTTCAGAAAACAGA
GATCTTAGGCAGGTTCAAGAATTATCAAAGAAAACAGACTCGGATGCCATTGTGCATTCT
GCTTTTAACTCAGACACTAATAGAGCTCCTCAGAATAGCTCTCCTTTTTCCAAACATCAT
ACTGAAATTTCAGTGTCAACTAATGAGCAGGCAATTGCTGTAGAGAATGCAGTTACTACC
ATGGCTAGCCAACCTATTTCTACGAAATCAGAAAATATTTGTAATTCAACAAGAGAAATC
CAGGGTATTGAGAAGCACCCTTATCCTGAGTCTAAACCTGAAGAAGTTTCCAGAAGTTCA
GGAATAGTGACATCAGGTAGTAGAAAAGAAAGATGCATAGGCCAGATTTTCCAAACAGAA
GAGTATAGTGTGGAAAAATCTCTAGGGCCAATGATTTTAATCAACAAACCTCTTGAGAAT
ATGGAAGAAGCAAGGCATGAAAATGAAGGATTAGTAAGCTCTGGGCAATCACTATACACT
TCAGGTGAAAAGGAATCTGACAGCTCTGCTTCTACTAGTCTTCCTGTTGAGGAAAGTCAA
GCTCAAGGCAACGAATCTTTGTTTTCAAAATATACCAACTCTAAAATACCTTATTTCCTT
TTGTTTCTGATATTTCTTATAACTGTCTACCATTATGACTTAATGATTGGCTTGACATTC
TACGTTTTGTCATTGTCCTGGCTATCCTGGGAAGAGGGTAGACAAAAAGAGTCTGTCAAA
AAGAAG

Protein sequence predicted based on nucleotide sequence

MEPSEVPSQISKDNFLEVPNLSDSLCEDEEVTFQPGFSPQPSRRGSDSSEDIYLDTPSSG
TRRVSFADSFGFNLVSVKEFDCWELPSASTTFDLGTDIFHTEEYVLAPLFDLPSSKEDLM
QQLQIQKAILESTESLLGSTSIKGIIRVLNVSFEKLVYVRMSLDDWQTHYDILAEYVPNS
CDGETDQFSFKIVLVPPYQKDGSKVEFCIRYETSVGTFWSNNNGTNYTFICQKKEQEPEP
VKPWKEVPNRQIKGCLKVKSSKEESSVTSEENNFENPKNTDTYIPTIICSHEDKEDLEAS
NRNVKDVNREHDEHNEKELELMINQHLIRTRSTASRDERNTFSTDPVNFPNKAEGLEKKQ
IHGEICTDLFQRSLSPSSSAESSVKGDFYCNEKYSSGDDCTHQPSEETTSNMGEIKPSLG
DTSSDELVQLHTGSKEVLDDNANPAHGNGTVQIPCPSSDQLMAGNLNKKHEGGAKNIEVK
DLGCLRRDFHSDTSACLKESTEEGSSKEDYYGNGKDDEEQRIYLGVNEKQRKNFQTILHD
QERKMGNPKISVAGIGASNRDLATLLSEHTAIPTRAITADVSHSPRTNLSWEEAVLTPEH
HHLTSEGSALGGITGQVCSSRTGNVLRNDYLFQVEEKSGGINSEDQDNSPQHKQSWNVLE
SQGKSRENKTNITEHIKGQTDCEDVWGKRDNTRSLKATTEELFTCQETVCCELSSLADHG
ITEKAEAGTAYIIKTTSESTPESMSAREKAIIAKLPQETARSDRPIEVKETAFDPHEGRN
DDSHYTLCQRDTVGVIYDNDFEKESRLGICNVRVDEMEKEETMSMYNPRKTHDREKCGTG
NITSVEESSWVITEYQKATSKLDLQLGMLPTDKTVFSENRDLRQVQELSKKTDSDAIVHS
AFNSDTNRAPQNSSPFSKHHTEISVSTNEQAIAVENAVTTMASQPISTKSENICNSTREI
QGIEKHPYPESKPEEVSRSSGIVTSGSRKERCIGQIFQTEEYSVEKSLGPMILINKPLEN
MEEARHENEGLVSSGQSLYTSGEKESDSSASTSLPVEESQAQGNESLFSKYTNSKIPYFL
LFLIFLITVYHYDLMIGLTFYVLSLSWLSWEEGRQKESVKKK

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 3400
Start on reference sequence 35
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable