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ZNF284 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF284
Gene Name:  zinc finger protein 284
Sequence             
Original Clone ID: FLH269558.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223.1               Format:  CLOSED
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00082783

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1782nts         Open reading frame : 1 to 1782
Reference Sequence Alignment

ATGACCATGTTCAAGGAGGCAGTGACCTTCAAGGATGTGGCTGTGGTCTTCACCGAGGAG
GAGCTGGGGCTGCTGGACGTTTCCCAGAGGAAGCTGTATCGAGATGTCATGCTGGAGAAC
TTCAGAAACCTGCTATCAGTGGGGCATCAACTTTCCCACCGAGATACTTTTCACTTCCAA
AGAGAAGAAAAGTTTTGGATCATGGAGACAGCAACCCAAAGAGAAGGGAATTCAGGAGGC
AAGATCCAAACTGAGTTGGAGTCTGTTCCAGAAACAGGACCACATGAAGAGTGGTCTTGC
CAGCAAATCTGGGAACAAACTGCAAGTGAGTTAACTAGACCTCAAGACTCCATAAGTAGC
TCTCAGTTCTCCACACAAGGTGATGTCCCCTCCCAGGTTGACGCAGGACTATCTATAATT
CACATAGGAGAGACACCTTCTGAGCATGGGAAGTGTAAAAAATTCTTCAGTGATGTCTCC
ATCCTTGATCTTCATCAACAATTACACTCAGGAAAGATATCCCATACATGTAATGAGTAC
AGGAAGAGATTCTGTTATAGCTCAGCTCTTTGTCTTCATCAGAAAGTTCACATGGGAGAG
AAACGCTATAAGTGTGATGTGTGTAGTAAGGCATTTAGTCAGAACTCACAACTGCAAACT
CATCAGAGAATCCACACTGGAGAGAAACCATTCAAATGTGAGCAGTGTGGGAAAAGTTTC
AGCCGTAGATCAGGAATGTATGTTCATTGCAAATTACACACAGGAGAAAAACCTCATATT
TGTGAGGAATGTGGGAAGGCCTTCATTCACAATTCCCAGCTTCGGGAACATCAAAGAATC
CATACTGGGGAGAAGCCATTCAAATGTTATATATGTGGTAAGAGCTTCCATAGTAGATCA
AATCTTAATAGGCATTCCATGGTCCACATGCAAGAGAAATCATTTAGATGTGATACCTGT
AGTAATAGCTTTGGTCAGAGATCAGCACTTAATAGTCATTGCATGGACCACACAAAAGAG
AAACTATACAAATGTGAAGAATGTGGAAGGAGCTTCACTTGTAGGCAAGATCTTTGTAAG
CATCAGATGGACCATACAGGAGACAAACCATATAATTGTAATGTATGTGGGAAGGGCTTC
AGGTGGTCCTCATGTCTTTCAAGACATCAGCGGGTCCACAATGGAGAAACAACATTCAAG
TGCGACGGATGTGGGAAGAGATTTTATATGAATTCACAGGGCCATTCACATCAGAGAGCC
TATAGAGAAGAAGAACTGTATAAATGTCAGAAGTGTGGGAAGGGCTACATTAGTAAGTTT
AATCTTGACTTGCACCAGAGGGTCCACACGGGAGAGAGACCTTATAATTGTAAGGAATGT
GGAAAGAGCTTCAGGTGGGCCTCAGGTATTTTGAGACATAAGAGACTCCATACTGGAGAA
AAACCATTCAAATGTGAAGAGTGTGGGAAGAGGTTTACTGAGAATTCAAAACTTCGTTTC
CATCAAAGAATTCACACTGGAGAAAAGCCTTACAAATGTGAGGAGTGTGGAAAGGGATTC
AGATGGGCCTCAACTCATCTAACCCATCAAAGACTCCACAGCAGAGAAAAACTATTCCAA
TGTGAGGATTGTGGGAAGAGCAGTGAGCACAGTTCATGCCTTCAAGACCAACAAAGCGAC
CACAGTGGAGAAAAAACATCCAAATGTGAGGACTGTGGGAAGCGCTACGAGAGGCGCTTG
AATCTAGATATGATTTTATCATTATTTTTAAATGATATATAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1998
Start on reference sequence 217
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TAAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF284
Symbol Nomenclature : ZNF284
SYNONYM : ZNF284L
Full Nomenclature : zinc finger protein 284
GENEID : 342909
GI : 162317721
GenBank Accession : BC156663
HGNC : 13078
Target GenBank: NM_001037813

 

Reference Sequence Alignment

  

HsCD00082783        ATGACCATGTTCAAGGAGGCAGTGACCTTCAAGGATGTGGCTGTGGTCTTCACCGAGGAG
XM_005258859.1      ATGACCATGTTCAAGGAGGCAGTGACCTTCAAGGATGTGGCTGTGGTCTTCACCGAGGAG
NM_001037813.2      ATGACCATGTTCAAGGAGGCAGTGACCTTCAAGGATGTGGCTGTGGTCTTCACCGAGGAG
                    ************************************************************

HsCD00082783        GAGCTGGGGCTGCTGGACGTTTCCCAGAGGAAGCTGTATCGAGATGTCATGCTGGAGAAC
XM_005258859.1      GAGCTGGGGCTGCTGGACGTTTCCCAGAGGAAGCTGTATCGAGATGTCATGCTGGAGAAC
NM_001037813.2      GAGCTGGGGCTGCTGGACGTTTCCCAGAGGAAGCTGTATCGAGATGTCATGCTGGAGAAC
                    ************************************************************

HsCD00082783        TTCAGAAACCTGCTATCAGTGGGGCATCAACTTTCCCACCGAGATACTTTTCACTTCCAA
XM_005258859.1      TTCAGAAACCTGCTATCAGTGGGGCATCAACTTTCCCACCGAGATACTTTTCACTTCCAA
NM_001037813.2      TTCAGAAACCTGCTATCAGTGGGGCATCAACTTTCCCACCGAGATACTTTTCACTTCCAA
                    ************************************************************

HsCD00082783        AGAGAAGAAAAGTTTTGGATCATGGAGACAGCAACCCAAAGAGAAGGGAATTCAGGAGGC
XM_005258859.1      AGAGAAGAAAAGTTTTGGATCATGGAGACAGCAACCCAAAGAGAAGGGAATTCAGGAGGC
NM_001037813.2      AGAGAAGAAAAGTTTTGGATCATGGAGACAGCAACCCAAAGAGAAGGGAATTCAGGAGGC
                    ************************************************************

HsCD00082783        AAGATCCAAACTGAGTTGGAGTCTGTTCCAGAAACAGGACCACATGAAGAGTGGTCTTGC
XM_005258859.1      AAGATCCAAACTGAGTTGGAGTCTGTTCCAGAAACAGGACCACATGAAGAGTGGTCTTGC
NM_001037813.2      AAGATCCAAACTGAGTTGGAGTCTGTTCCAGAAACAGGACCACATGAAGAGTGGTCTTGC
                    ************************************************************

HsCD00082783        CAGCAAATCTGGGAACAAACTGCAAGTGAGTTAACTAGACCTCAAGACTCCATAAGTAGC
XM_005258859.1      CAGCAAATCTGGGAACAAACTGCAAGTGAGTTAACTAGACCTCAAGACTCCATAAGTAGC
NM_001037813.2      CAGCAAATCTGGGAACAAACTGCAAGTGAGTTAACTAGACCTCAAGACTCCATAAGTAGC
                    ************************************************************

HsCD00082783        TCTCAGTTCTCCACACAAGGTGATGTCCCCTCCCAGGTTGACGCAGGACTATCTATAATT
XM_005258859.1      TCTCAGTTCTCCACACAAGGTGATGTCCCCTCCCAGGTTGACGCAGGACTATCTATAATT
NM_001037813.2      TCTCAGTTCTCCACACAAGGTGATGTCCCCTCCCAGGTTGACGCAGGACTATCTATAATT
                    ************************************************************

HsCD00082783        CACATAGGAGAGACACCTTCTGAGCATGGGAAGTGTAAAAAATTCTTCAGTGATGTCTCC
XM_005258859.1      CACATAGGAGAGACACCTTCTGAGCATGGGAAGTGTAAAAAATTCTTCAGTGATGTCTCC
NM_001037813.2      CACATAGGAGAGACACCTTCTGAGCATGGGAAGTGTAAAAAATTCTTCAGTGATGTCTCC
                    ************************************************************

HsCD00082783        ATCCTTGATCTTCATCAACAATTACACTCAGGAAAGATATCCCATACATGTAATGAGTAC
XM_005258859.1      ATCCTTGATCTTCATCAACAATTACACTCAGGAAAGATATCCCATACATGTAATGAGTAC
NM_001037813.2      ATCCTTGATCTTCATCAACAATTACACTCAGGAAAGATATCCCATACATGTAATGAGTAC
                    ************************************************************

HsCD00082783        AGGAAGAGATTCTGTTATAGCTCAGCTCTTTGTCTTCATCAGAAAGTTCACATGGGAGAG
XM_005258859.1      AGGAAGAGATTCTGTTATAGCTCAGCTCTTTGTCTTCATCAGAAAGTTCACATGGGAGAG
NM_001037813.2      AGGAAGAGATTCTGTTATAGCTCAGCTCTTTGTCTTCATCAGAAAGTTCACATGGGAGAG
                    ************************************************************

HsCD00082783        AAACGCTATAAGTGTGATGTGTGTAGTAAGGCATTTAGTCAGAACTCACAACTGCAAACT
XM_005258859.1      AAACGCTATAAGTGTGATGTGTGTAGTAAGGCATTTAGTCAGAACTCACAACTGCAAACT
NM_001037813.2      AAACGCTATAAGTGTGATGTGTGTAGTAAGGCATTTAGTCAGAACTCACAACTGCAAACT
                    ************************************************************

HsCD00082783        CATCAGAGAATCCACACTGGAGAGAAACCATTCAAATGTGAGCAGTGTGGGAAAAGTTTC
XM_005258859.1      CATCAGAGAATCCACACTGGAGAGAAACCATTCAAATGTGAGCAGTGTGGGAAAAGTTTC
NM_001037813.2      CATCAGAGAATCCACACTGGAGAGAAACCATTCAAATGTGAGCAGTGTGGGAAAAGTTTC
                    ************************************************************

HsCD00082783        AGCCGTAGATCAGGAATGTATGTTCATTGCAAATTACACACAGGAGAAAAACCTCATATT
XM_005258859.1      AGCCGTAGATCAGGAATGTATGTTCATTGCAAATTACACACAGGAGAAAAACCTCATATT
NM_001037813.2      AGCCGTAGATCAGGAATGTATGTTCATTGCAAATTACACACAGGAGAAAAACCTCATATT
                    ************************************************************

HsCD00082783        TGTGAGGAATGTGGGAAGGCCTTCATTCACAATTCCCAGCTTCGGGAACATCAAAGAATC
XM_005258859.1      TGTGAGGAATGTGGGAAGGCCTTCATTCACAATTCCCAGCTTCGGGAACATCAAAGAATC
NM_001037813.2      TGTGAGGAATGTGGGAAGGCCTTCATTCACAATTCCCAGCTTCGGGAACATCAAAGAATC
                    ************************************************************

HsCD00082783        CATACTGGGGAGAAGCCATTCAAATGTTATATATGTGGTAAGAGCTTCCATAGTAGATCA
XM_005258859.1      CATACTGGGGAGAAGCCATTCAAATGTTATATATGTGGTAAGAGCTTCCATAGTAGATCA
NM_001037813.2      CATACTGGGGAGAAGCCATTCAAATGTTATATATGTGGTAAGAGCTTCCATAGTAGATCA
                    ************************************************************

HsCD00082783        AATCTTAATAGGCATTCCATGGTCCACATGCAAGAGAAATCATTTAGATGTGATACCTGT
XM_005258859.1      AATCTTAATAGGCATTCCATGGTCCACATGCAAGAGAAATCATTTAGATGTGATACCTGT
NM_001037813.2      AATCTTAATAGGCATTCCATGGTCCACATGCAAGAGAAATCATTTAGATGTGATACCTGT
                    ************************************************************

HsCD00082783        AGTAATAGCTTTGGTCAGAGATCAGCACTTAATAGTCATTGCATGGACCACACAAAAGAG
XM_005258859.1      AGTAATAGCTTTGGTCAGAGATCAGCACTTAATAGTCATTGCATGGACCACACAAAAGAG
NM_001037813.2      AGTAATAGCTTTGGTCAGAGATCAGCACTTAATAGTCATTGCATGGACCACACAAAAGAG
                    ************************************************************

HsCD00082783        AAACTATACAAATGTGAAGAATGTGGAAGGAGCTTCACTTGTAGGCAAGATCTTTGTAAG
XM_005258859.1      AAACTATACAAATGTGAAGAATGTGGAAGGAGCTTCACTTGTAGGCAAGATCTTTGTAAG
NM_001037813.2      AAACTATACAAATGTGAAGAATGTGGAAGGAGCTTCACTTGTAGGCAAGATCTTTGTAAG
                    ************************************************************

HsCD00082783        CATCAGATGGACCATACAGGAGACAAACCATATAATTGTAATGTATGTGGGAAGGGCTTC
XM_005258859.1      CATCAGATGGACCATACAGGAGACAAACCATATAATTGTAATGTATGTGGGAAGGGCTTC
NM_001037813.2      CATCAGATGGACCATACAGGAGACAAACCATATAATTGTAATGTATGTGGGAAGGGCTTC
                    ************************************************************

HsCD00082783        AGGTGGTCCTCATGTCTTTCAAGACATCAGCGGGTCCACAATGGAGAAACAACATTCAAG
XM_005258859.1      AGGTGGTCCTCATGTCTTTCAAGACATCAGCGGGTCCACAATGGAGAAACAACATTCAAG
NM_001037813.2      AGGTGGTCCTCATGTCTTTCAAGACATCAGCGGGTCCACAATGGAGAAACAACATTCAAG
                    ************************************************************

HsCD00082783        TGCGACGGATGTGGGAAGAGATTTTATATGAATTCACAGGGCCATTCACATCAGAGAGCC
XM_005258859.1      TGCGACGGATGTGGGAAGAGATTTTATATGAATTCACAGGGCCATTCACATCAGAGAGCC
NM_001037813.2      TGCGACGGATGTGGGAAGAGATTTTATATGAATTCACAGGGCCATTCACATCAGAGAGCC
                    ************************************************************

HsCD00082783        TATAGAGAAGAAGAACTGTATAAATGTCAGAAGTGTGGGAAGGGCTACATTAGTAAGTTT
XM_005258859.1      TATAGAGAAGAAGAACTGTATAAATGTCAGAAGTGTGGGAAGGGCTACATTAGTAAGTTT
NM_001037813.2      TATAGAGAAGAAGAACTGTATAAATGTCAGAAGTGTGGGAAGGGCTACATTAGTAAGTTT
                    ************************************************************

HsCD00082783        AATCTTGACTTGCACCAGAGGGTCCACACGGGAGAGAGACCTTATAATTGTAAGGAATGT
XM_005258859.1      AATCTTGACTTGCACCAGAGGGTCCACACGGGAGAGAGACCTTATAATTGTAAGGAATGT
NM_001037813.2      AATCTTGACTTGCACCAGAGGGTCCACACGGGAGAGAGACCTTATAATTGTAAGGAATGT
                    ************************************************************

HsCD00082783        GGAAAGAGCTTCAGGTGGGCCTCAGGTATTTTGAGACATAAGAGACTCCATACTGGAGAA
XM_005258859.1      GGAAAGAGCTTCAGGTGGGCCTCAGGTATTTTGAGACATAAGAGACTCCATACTGGAGAA
NM_001037813.2      GGAAAGAGCTTCAGGTGGGCCTCAGGTATTTTGAGACATAAGAGACTCCATACTGGAGAA
                    ************************************************************

HsCD00082783        AAACCATTCAAATGTGAAGAGTGTGGGAAGAGGTTTACTGAGAATTCAAAACTTCGTTTC
XM_005258859.1      AAACCATTCAAATGTGAAGAGTGTGGGAAGAGGTTTACTGAGAATTCAAAACTTCGTTTC
NM_001037813.2      AAACCATTCAAATGTGAAGAGTGTGGGAAGAGGTTTACTGAGAATTCAAAACTTCGTTTC
                    ************************************************************

HsCD00082783        CATCAAAGAATTCACACTGGAGAAAAGCCTTACAAATGTGAGGAGTGTGGAAAGGGATTC
XM_005258859.1      CATCAAAGAATTCACACTGGAGAAAAGCCTTACAAATGTGAGGAGTGTGGAAAGGGATTC
NM_001037813.2      CATCAAAGAATTCACACTGGAGAAAAGCCTTACAAATGTGAGGAGTGTGGAAAGGGATTC
                    ************************************************************

HsCD00082783        AGATGGGCCTCAACTCATCTAACCCATCAAAGACTCCACAGCAGAGAAAAACTATTCCAA
XM_005258859.1      AGATGGGCCTCAACTCATCTAACCCATCAAAGACTCCACAGCAGAGAAAAACTATTCCAA
NM_001037813.2      AGATGGGCCTCAACTCATCTAACCCATCAAAGACTCCACAGCAGAGAAAAACTATTCCAA
                    ************************************************************

HsCD00082783        TGTGAGGATTGTGGGAAGAGCAGTGAGCACAGTTCATGCCTTCAAGACCAACAAAGCGAC
XM_005258859.1      TGTGAGGATTGTGGGAAGAGCAGTGAGCACAGTTCATGCCTTCAAGACCAACAAAGCGAC
NM_001037813.2      TGTGAGGATTGTGGGAAGAGCAGTGAGCACAGTTCATGCCTTCAAGACCAACAAAGCGAC
                    ************************************************************

HsCD00082783        CACAGTGGAGAAAAAACATCCAAATGTGAGGACTGTGGGAAGCGCTACGAGAGGCGCTTG
XM_005258859.1      CACAGTGGAGAAAAAACATCCAAATGTGAGGACTGTGGGAAGCGCTACGAGAGGCGCTTG
NM_001037813.2      CACAGTGGAGAAAAAACATCCAAATGTGAGGACTGTGGGAAGCGCTACGAGAGGCGCTTG
                    ************************************************************

HsCD00082783        AATCTAGATATGATTTTATCATTATTTTTAAATGATATATAA
XM_005258859.1      AATCTAGATATGATTTTATCATTATTTTTAAATGATATATAA
NM_001037813.2      AATCTAGATATGATTTTATCATTATTTTTAAATGATATATAA
                    ******************************************

Protein

SMART domain : KRAB : krueppel associated box
SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q2VY69

Clone

Cloning Information : 269558
HIP Master Clone ID : 201060
IMAGE ID : 100062153
Original Clone ID : FLH269558.01X

Organism


TAX_ID : 9606
Species Specific ID: 342909

Genome

Chromosome : 19
Map Location : 19q13.31

Reagents

Labome : ZNF284-antibody