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U2af1-rs2 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  Zrsr2
Gene Name:  zinc finger (CCCH type), RNA binding motif and serine/arginine rich 2
Sequence             
Original Clone ID: FLH270291.01L
Keyword: None
Species: Mus musculus
Type: cDNA
Vector Name: pENTR223.1               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
MmCD00083217

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1623nts         Open reading frame : 1 to 1623

ATGGAAACGGCGGGGGCTACGGCGGACGCTACGGCGGGGCCGCAGAAACTGAGCCGTAAG
AAGTACTTGGCCCTCCGGAAGAAGGAGAGACGTAAGAGGCGACGGCAGGCGCTCGCTCGG
CTCAGAGAGGCAGAATTGGCACAGAAAGAGGAGGAAGAAGATCCTCTTGCTGAAGAAAAA
CGACTAGAAGAAGAAAGGTTGTTAGAGGAAGAGAGGCAGAGACTGCATGAAGAATGGTTA
CTGAGGGAAGAAAAGGCACAAGAAGAATTCAGGGCAAAGAAGAAAAAAGAAGAGGAAGCT
CGAAAACGGAAGGAAGAACTAGAGAGAAAGTTAAAGGCAGAATGGGAAGAACAGCAGAGA
AAAGAGCGAGAGGAAGAGGAGCAGAAACGACAAGAGAAGAGAGAAAGAGAGGAAGCTGTG
CAGAAGATGCTGGATCAGGCTGAAAATGAGCTGGAAAATGGTGGCACCTGGCAGAACCCA
GAACCGCCCATGGATATAAGAGTACTGGAGAAAGATCGAGCCAACTGTCCGTTCTACAGC
AAAACGGGAGCTTGCAGATTTGGAGATAGGTGTTCACGTAAACACAACTTCCCCACATCA
AGTCCCACCTTGCTGATTAAGGGCATGTTTACAACATTTGGAATGGAGCAGTGTCGGAGG
GATGACTATGACCCTGACTCAAGCCTAGAATTCAGTGAAGAAGAGATCTACCAACAGTTC
CTAGACTTCTATTATGATGTCCTGCCTGAATTCAAGAGTGTGGGAAAAGTGATTCAGTTT
AAGGTCAGCTGCAACCTGGAACCTCATCTGAGGGGCAATGTGTATGTTCAGTACCAGTCG
GAAGAAGACTGTCAAGCAGCCTTTTCTGTTTTTAATGGACGATGGTATGCAGGACGACAG
CTCCAGTGTGAATTCTGCCCAGTGACCCGGTGGAAAATGGCAATTTGTGGCTTATTTGAA
GTCCAGCAGTGTCCAAGAGGAAAGCACTGCAACTTTCTTCACGTGTTCAGAAATCCCAAC
AATGAATATAGGGACGCTAATAGAGACCTCTACCCGTCTCCAGATTGGACTAGCTCCTCC
TTCGGTAAGAATTCAGAGAGGAGGGAAAGGGCCAGTCACTATGATGAATACTATGGCAGG
TCAAGGAGGAGGAGGAGGAGCCCGAGTCCCGACTTCTACAAGAGAAATGGCGAGTCTGAC
AGGAAGAGCAGTAGCAGACACAGGGTGAAGAAATCTCACAGATATGGAATGAAGAGTCGT
GAGAGGCGCAGTTCACCCAGTAGAAGAAGAAAGGACCACAGTCCGGGCCCCTGGAGCCAG
AGCAGGAGGAGCCACCGCAGCAGGAGTCGCAGTCGCAGCCGCAGCCGCAGCCGCAGTCGG
ACCCGTAGCCGAAGCCGGGGCCGGGGCCGGAGCAGGAGCCGGAGCCGGAGCAGGAGCAGG
GGCCGGAGCAGGAGCAGGGGCCGGGGCAGCGGCAGGGGCAGGGGCAGGGGCAGGGGCAGG
GGCAGGAACCAGAGCAGGAGTTGGAGTCAAAGCCGGAGCCGGAGTAGTAGCTCGTCAAGG
TCCAGAAGTCGTGGCAGGAGGTCAGGTAGTAGAGACAAAACCACCCAGAGTCCCAAATCT
AAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1898
Start on reference sequence 273
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
gtacaaaaaagcagaagGGCCGTCAAGGCCCACC

3' Linker Sequence:
TCAGGCCTCATGGgcccagctttcttg

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223.1

pENTR223.1 in advanced viewed

Synonyms: pDONR-223.1, pENTR223-star-Sfi
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp): 4311
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 1265 1277
bacterial origin ori bacterial origin of replication 1 534
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 1312 1617
primer site M13 F M13 forward primer for insert sequencing 1113 1129
primer site T7 T7 Forward primer for insert sequencing 2902 2921
primer site M13 reverse M13 reverse primer for insert sequencing 2926 2942
recombination site attL1 attL1 recombination site 1165 1264
recombination site attL2 attL2 recombination site 2786 2885
selectable marker SpnR spectinomycin resistance gene 3154 4164
selectable marker CmR chlR (no insert form only; lost in with insert form) 1959 2618

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : Zrsr2
Symbol Nomenclature : Zrsr2
SYNONYM : 35kDa; 5031411E02Rik; A230052C13Rik; C77286; U2af1-rs2; URP
Designation : U2 small nuclear ribonucleoprotein auxiliary factor (U2AF), 35 kDa, related sequence 2|U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2|U2(RNU2) small nuclear RNA auxiliary factor 1-like 2|U2AF35-related protein
Full Nomenclature : zinc finger (CCCH type), RNA binding motif and serine/arginine rich 2
GENEID : 22184
Locus Tag : RP23-391L6.2
GI : 162319137
GenBank Accession : BC156404
Mouse Genome Informatics : 103287
Vega : OTTMUSG00000019507
Target GenBank: NM_009453

 

Protein

SMART domain : ZnF_C3H1 : zinc finger
SMART domain : RRM : RNA recognition motif
SMART domain : RRM_1 : RNA recognition motif
UniProt : Q8BG24
UniProt : B1B0E9
UniProt : B1B0E8

Clone

Cloning Information : 270291
HIP Master Clone ID : 201793
IMAGE ID : 100061959
Original Clone ID : FLH270291.01L

Organism


TAX_ID : 10090
Species Specific ID: 22184

Genome

Chromosome : X
Map Location : X F5|X 75.99 cM
Ensembl : ENSMUSG00000031370

Reagents

Labome : U2af1-rs2-antibody