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KIAA0318 (Homo sapiens) in pVP16 (His and MBP-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  RIMBP2
Gene Name:  RIMS binding protein 2
Sequence             
Original Clone ID: 1025.1.64_GO.34963
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pVP16               Format:  CLOSED
Source: Center for Eukaryotic Structural Genomics
Description: None
Comments: His8-MBP fusion prtotein with TEV protease cleavage site is produced; after cleavage the protein obtained will be the translated CDS with the addition of SER at the N-term
Mutation/
Discrepancy :
No / No
Publications: None
Authors: University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
HsCD00083683

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 2012nts         Open reading frame : 28 to 1986
Reference Sequence Alignment

ACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCAATGGCCTAGCC
ACCTCCCTCGGCAAAGGTCAGGAGAGCGCTATTGGAGGCAGCTCTGCGATCGGTGAATAT
ATCCGGCCCCTTCCGCAGCCTGGTGACAGGCCGGAGCCTCTGTCCGCCAAGCCCACCTTC
CTGTCGAGATCCGGTAGCGCAAGATGCAGATCTGAGTCAGACATGGAGAATGAACGGAAT
TCCAATACCTCCAAGCAGAGATACTCGGGGAAGGTCCACCTCTGTGTTGCCCGCTATAGT
TACAACCCCTTCGATGGACCGAACGAGAACCCCGAAGCTGAGCTGCCCCTCACGGCGGGA
AAATACCTCTACGTCTATGGAGACATGGATGAGGATGGGTTCTATGAAGGAGAGCTCCTC
GATGGCCAGAGGGGTCTGGTGCCCTCCAACTTCGTGGACTTTGTGCAGGACAACGAGTCG
CGGTTGGCAAGCACGCTGGGGAACGAGCAGGATCAGAACTTCATCAACCATTCCGGCATC
GGCCTGGAGGGAGAGCACATCCTGGACCTCCACTCCCCAACCCACATAGATGCGGGCATC
ACCGACAACAGTGCCGGGACCCTGGACGTGAACATCGACGACATCGGAGAAGACATCGTG
CCTTACCCTAGAAAAATCACCCTCATCAAACAACTCGCCAAAAGTGTTATTGTGGGCTGG
GAGCCCCCGGCGGTGCCACCAGGATGGGGAACGGTGAGCAGCTACAACGTCCTGGTGGAC
AAGGAGACACGCATGAACCTCACGCTGGGGAGCAGAACTAAAGCCCTCATCGAGAAGCTC
AACATGGCAGCCTGCACCTACCGCATCTCCGTGCAGTGCGTCACCAGCAGGGGCAGCTCA
GATGAGCTGCAGTGCACGCTGCTGGTGGGCAAGGACGTGGTGGTGGCCCCCTCCCACCTG
CGGGTGGACAACATCACGCAGATCTCCGCCCAGCTCTCCTGGCTACCCACCAACAGCAAC
TACAGCCACGTCATCTTCCTCAACGAGGAGGAGTTCGACATCGTCAAGGCCGCCAGGTAC
AAGTACCAGTTCTTCAATCTCAGGCCCAACATGGCCTATAAGGTGAAGGTTCTGGCCAAA
CCCCACCAGATGCCGTGGCAGCTCCCGCTGGAGCAAAGGGAGAAGAAGGAGGCCTTTGTG
GAGTTCTCCACGTTGCCTGCAGGACCCCCAGCACCCCCACAAGATGTTACCGTCCAGGCT
GGGGTGACCCCCGCCACCATCCGGGTCTCCTGGAGACCACCTGTGCTGACGCCCACCGGG
CTGTCCAATGGCGCAAACGTTACCGGCTACGGCGTGTATGCCAAAGGGCAGAGGGTGGCT
GAAGTCATCTTCCCCACGGCAGACAGCACGGCCGTGGAGCTTGTGCGGCTGCGGAGCCTG
GAGGCCAAGGGCGTGACCGTGCGGACCCTCTCCGCCCAGGGCGAGTCCGTGGACTCTGCA
GTTGCTGCCGTTCCCCCCGAGCTCCTGGTGCCTCCTACCCCCCACCCGAGACCTGCACCC
CAATCAAAGCCATTAGCAAGTTCTGGAGTCCCCGAAACCAAAGACGAGCACCTGGGTCCC
CACGCCAGGATGGATGAGGCCTGGGAGCAGAGCCGTGCACCTGGCCCTGTGCATGGGCAC
ATGCTGGAGCCGCCCGTGGGCCCCGGAAGGCGGTCGCCCTCACCCAGCCGCATCCTGCCA
CAGCCACAGGGCACCCCGGTGTCCACCACCGTCGCCAAGGCCATGGCCCGGGAGGCCGCG
CAGAGGGTGGCCGAGAGCAGCAGGTTAGAGAAAAGGAGCGTCTTCCTAGAGAGAAGCAGC
GCGGGGCAGTACGCCGCCTCAGACGAGGAGGACGCCTATGACTCTCCAGACTTCAAGAGG
AGGGGCGCCTCGGTGGACGACTTCCTGAAAGGCTCTGAACTTGGCAAGCAGATGGTCTCC
TAATAGGACCCAGCTTTCTTGTACAAAGTGGT


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 1959
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ACAAGTTTGTACAAAAAAGCAGGCTCC

3' Linker Sequence:
TAGGACCCAGCTTTCTTGTACAAAGTGGT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pVP16

pVP16 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  MBP Forward
Reverse:  pQE Reverse
Description: Bacterial (E. coli) expression, adds N-terminal 8xHis and MBP, adds TEV site on 5p end of insert; ampicillin resistance in bacteria; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette.
Comments: TEV protease cleavage site is "partial" and not functional. The TEV cleavage site must be added to the insert by PCR, which allows amino acids encoded by the attB1 site to be cut off after protein expression. Gateway cloning into this vector will remove the Gateway cassette
Size (bp): 7631
Parent Vector: None
Empty Vector: EvNO00084279
Properties: Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name: Center for Eukaryotic Structural Genomics
Publications: PMID: 15750721
Title: Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type bacterial origin of replication 6471 5789
negative selection marker ccdB ccdB death cassette gene (lost in with-insert form) 2643 2852
promoter T5 T5 promoter 5 54
recombination site AttR1 AttR1 recombination site 1316 1440
recombination site AttR2 AttR2 recombination site 3020 2896
repressor protein gene LacI LacI coding region 4132 5211
selectable marker AmpR ampicillin resistance gene 7228 6569
selectable marker CmR chloramphenicol resistance gene (CmR) (Lost in with-insert form) 1549 2205
tag His N-terminal 8xHis tag 128 145
tag MBP N-terminal MBP 148 1246

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RIMBP2
Symbol Nomenclature : RIMBP2
SYNONYM : RBP2; RIM-BP2; RP11-662M24.2
Designation : RIM binding protein 2|RIMS-binding protein 2
Full Nomenclature : RIMS binding protein 2
GENEID : 23504
HGNC : 30339
MIM : 611602
Vega : OTTHUMG00000168385
Target GenBank: BC007632

 

Reference Sequence Alignment

  

HsCD00083683      ------------------------------------------------------------
NM_015347.4       ATGCGAGAGGCGGCTGAACGGCGGCAGCAGCTGCAGTTGGAGCATGACCAGGCCCTGGCT
                                                                              

HsCD00083683      ------------------------------------------------------------
NM_015347.4       GTTCTCAGTGCCAAGCAGCAGGAAATTGACCTTCTGCAGAAGTCCAAGGTTCGAGAGCTG
                                                                              

HsCD00083683      ------------------------------------------------------------
NM_015347.4       GAAGAGAAATGCCGGACTCAAAGTGAGCAGTTCAACCTGCTGTCCCGGGACCTGGAGAAG
                                                                              

HsCD00083683      ---------------------------------------------------ACAAGTTTG
NM_015347.4       TTCCGGCAGCACGCTGGCAAGATTGACCTGCTGGGTGGCAGCGCGGTGGCCCCCCTGGAC
                                                                     .*..   : 

HsCD00083683      TACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCAATGGCCTAGCCACCTCCCTC
NM_015347.4       ATCTCCACGGCCCCCAGCAAGCCTTTCCCACAGTTCATGAATGGCCTAGCCACCTCCCTC
                  ::*:..*..**.  *: *.*...  *  .. :  : :  *********************

HsCD00083683      GGCAAAGGTCAGGAGAGCGCTATTGGAGGCAGCTCTGCGATCGGTGAATATATCCGGCCC
NM_015347.4       GGCAAAGGTCAGGAGAGCGCTATTGGAGGCAGCTCTGCGATCGGTGAATATATCCGGCCC
                  ************************************************************

HsCD00083683      CTTCCGCAGCCTGGTGACAGGCCGGAGCCTCTGTCCGCCAAGCCCACCTTCCTGTCGAGA
NM_015347.4       CTTCCGCAGCCTGGTGACAGGCCGGAGCCTCTGTCCGCCAAGCCCACCTTCCTGTCGAGA
                  ************************************************************

HsCD00083683      TCCGGTAGCGCAAGATGCAGATCTGAGTCAGACATGGAGAATGAACGGAATTCCAATACC
NM_015347.4       TCCGGTAGCGCAAGATGCAGATCTGAGTCAGACATGGAGAATGAACGGAATTCCAATACC
                  ************************************************************

HsCD00083683      TCCAAGCAGAGATACTCGGGGAAGGTCCACCTCTGTGTTGCCCGCTATAGTTACAACCCC
NM_015347.4       TCCAAGCAGAGATACTCGGGGAAGGTCCACCTCTGTGTTGCCCGCTATAGTTACAACCCC
                  ************************************************************

HsCD00083683      TTCGATGGACCGAACGAGAACCCCGAAGCTGAGCTGCCCCTCACGGCGGGAAAATACCTC
NM_015347.4       TTCGATGGACCGAACGAGAACCCCGAAGCTGAGCTGCCCCTCACGGCGGGAAAATACCTC
                  ************************************************************

HsCD00083683      TACGTCTATGGAGACATGGATGAGGATGGGTTCTATGAAGGAGAGCTCCTCGATGGCCAG
NM_015347.4       TACGTCTATGGAGACATGGATGAGGATGGGTTCTATGAAGGAGAGCTCCTCGATGGCCAG
                  ************************************************************

HsCD00083683      AGGGGTCTGGTGCCCTCCAACTTCGTGGACTTTGTGCAGGACAACGAGTCGCGGTTGGCA
NM_015347.4       AGGGGTCTGGTGCCCTCCAACTTCGTGGACTTTGTGCAGGACAACGAGTCGCGGTTGGCA
                  ************************************************************

HsCD00083683      AGCACGCTGGGGAACGAGCAGGATCAGAACTTCATCAACCATTCCGGCATCGGCCTGGAG
NM_015347.4       AGCACGCTGGGGAACGAGCAGGATCAGAACTTCATCAACCATTCCGGCATCGGCCTGGAG
                  ************************************************************

HsCD00083683      GGAGAGCACATCCTGGACCTCCACTCCCCAACCCACATAGATGCGGGCATCACCGACAAC
NM_015347.4       GGAGAGCACATCCTGGACCTCCACTCCCCAACCCACATAGATGCGGGCATCACCGACAAC
                  ************************************************************

HsCD00083683      AGTGCCGGGACCCTGGACGTGAACATCGACGACATCGGAGAAGACATCGTGCCTTACCCT
NM_015347.4       AGTGCCGGGACCCTGGACGTGAACATCGACGACATCGGAGAAGACATCGTGCCTTACCCT
                  ************************************************************

HsCD00083683      AGAAAAATCACCCTCATCAAACAACTCGCCAAAAGTGTTATTGTGGGCTGGGAGCCCCCG
NM_015347.4       AGAAAAATCACCCTCATCAAACAACTCGCCAAAAGTGTTATTGTGGGCTGGGAGCCCCCG
                  ************************************************************

HsCD00083683      GCGGTGCCACCAGGATGGGGAACGGTGAGCAGCTACAACGTCCTGGTGGACAAGGAGACA
NM_015347.4       GCGGTGCCACCAGGATGGGGAACGGTGAGCAGCTACAACGTCCTGGTGGACAAGGAGACA
                  ************************************************************

HsCD00083683      CGCATGAACCTCACGCTGGGGAGCAGAACTAAAGCCCTCATCGAGAAGCTCAACATGGCA
NM_015347.4       CGCATGAACCTCACGCTGGGGAGCAGAACTAAAGCCCTCATCGAGAAGCTCAACATGGCA
                  ************************************************************

HsCD00083683      GCCTGCACCTACCGCATCTCCGTGCAGTGCGTCACCAGCAGGGGCAGCTCAGATGAGCTG
NM_015347.4       GCCTGCACCTACCGCATCTCCGTGCAGTGCGTCACCAGCAGGGGCAGCTCGGATGAGCTG
                  **************************************************.*********

HsCD00083683      CAGTGCACGCTGCTGGTGGGCAAGGACGTGGTGGTGGCCCCCTCCCACCTGCGGGTGGAC
NM_015347.4       CAGTGCACGCTGCTGGTGGGCAAGGACGTGGTGGTGGCCCCCTCCCACCTGCGGGTGGAC
                  ************************************************************

HsCD00083683      AACATCACGCAGATCTCCGCCCAGCTCTCCTGGCTACCCACCAACAGCAACTACAGCCAC
NM_015347.4       AACATCACGCAGATCTCCGCCCAGCTCTCCTGGCTACCCACCAACAGCAACTACAGCCAC
                  ************************************************************

HsCD00083683      GTCATCTTCCTCAACGAGGAGGAGTTCGACATCGTCAAGGCCGCCAGGTACAAGTACCAG
NM_015347.4       GTCATCTTCCTCAACGAGGAGGAGTTCGACATCGTCAAGGCCGCCAGGTACAAGTACCAG
                  ************************************************************

HsCD00083683      TTCTTCAATCTCAGGCCCAACATGGCCTATAAGGTGAAGGTTCTGGCCAAACCCCACCAG
NM_015347.4       TTCTTCAATCTCAGGCCCAACATGGCCTATAAGGTGAAGGTTCTGGCCAAACCCCACCAG
                  ************************************************************

HsCD00083683      ATGCCGTGGCAGCTCCCGCTGGAGCAAAGGGAGAAGAAGGAGGCCTTTGTGGAGTTCTCC
NM_015347.4       ATGCCGTGGCAGCTCCCGCTGGAGCAAAGGGAGAAGAAGGAGGCCTTTGTGGAGTTCTCC
                  ************************************************************

HsCD00083683      ACGTTGCCTGCAGGACCCCCAGCACCCCCACAAGATGTTACCGTCCAGGCTGGGGTGACC
NM_015347.4       ACGTTGCCTGCAGGACCCCCAGCACCCCCACAAGATGTTACCGTCCAGGCTGGGGTGACC
                  ************************************************************

HsCD00083683      CCCGCCACCATCCGGGTCTCCTGGAGACCACCTGTGCTGACGCCCACCGGGCTGTCCAAT
NM_015347.4       CCCGCCACCATCCGGGTCTCCTGGAGACCACCTGTGCTGACGCCCACCGGGCTGTCCAAT
                  ************************************************************

HsCD00083683      GGCGCAAACGTTACCGGCTACGGCGTGTATGCCAAAGGGCAGAGGGTGGCTGAAGTCATC
NM_015347.4       GGCGCAAACGTTACCGGCTACGGCGTGTATGCCAAAGGGCAGAGGGTGGCTGAAGTCATC
                  ************************************************************

HsCD00083683      TTCCCCACGGCAGACAGCACGGCCGTGGAGCTTGTGCGGCTGCGGAGCCTGGAGGCCAAG
NM_015347.4       TTCCCCACGGCAGACAGCACGGCCGTGGAGCTTGTGCGGCTGCGGAGCCTGGAGGCCAAG
                  ************************************************************

HsCD00083683      GGCGTGACCGTGCGGACCCTCTCCGCCCAGGGCGAGTCCGTGGACTCTGCAGTTGCTGCC
NM_015347.4       GGCGTGACCGTGCGGACCCTCTCCGCCCAGGGCGAGTCCGTGGACTCTGCAGTTGCTGCC
                  ************************************************************

HsCD00083683      GTTCCCCCCGAGCTCCTGGTGCCTCCTACCCCCCACCCGAGACCTGCACCCCAATCAAAG
NM_015347.4       GTTCCCCCCGAGCTCCTGGTGCCTCCTACCCCCCACCCGAGACCTGCACCCCAATCAAAG
                  ************************************************************

HsCD00083683      CCATTAGCAAGTTCTGGAGTCCCCGAAACCAAAGACGAGCACCTGGGTCCCCACGCCAGG
NM_015347.4       CCATTAGCAAGTTCTGGAGTCCCCGAAACCAAAGACGAGCACCTGGGTCCCCACGCCAGG
                  ************************************************************

HsCD00083683      ATGGATGAGGCCTGGGAGCAGAGCCGTGCACCTGGCCCTGTGCATGGGCACATGCTGGAG
NM_015347.4       ATGGATGAGGCCTGGGAGCAGAGCCGTGCACCTGGCCCTGTGCATGGGCACATGCTGGAG
                  ************************************************************

HsCD00083683      CCGCCCGTGGGCCCCGGAAGGCGGTCGCCCTCACCCAGCCGCATCCTGCCACAGCCACAG
NM_015347.4       CCGCCCGTGGGCCCCGGAAGGCGGTCGCCCTCACCCAGCCGCATCCTGCCACAGCCACAG
                  ************************************************************

HsCD00083683      GGCACCCCGGTGTCCACCACCGTCGCCAAGGCCATGGCCCGGGAGGCCGCGCAGAGGGTG
NM_015347.4       GGCACCCCGGTGTCCACCACCGTCGCCAAGGCCATGGCCCGGGAGGCCGCGCAGAGGGTG
                  ************************************************************

HsCD00083683      GCCGAGAGCAGCAGGTTAGAGAAAAGGAGCGTCTTCCTAGAGAGAAGCAGCGCGGGGCAG
NM_015347.4       GCCGAGAGCAGCAGGTTAGAGAAAAGGAGCGTCTTCCTAGAGAGAAGCAGCGCGGGGCAG
                  ************************************************************

HsCD00083683      TACGCCGCCTCAGACGAGGAGGACGCCTATGACTCTCCAGACTTCAAGAGGAGGGGCGCC
NM_015347.4       TACGCCGCCTCAGACGAGGAGGACGCCTATGACTCTCCAGACTTCAAGAGGAGGGGCGCC
                  ************************************************************

HsCD00083683      TCGGTGGACGACTTCCTGAAAGGCTCTGAACTTGGCAAGCAGATGGTCTCCTAATAGGAC
NM_015347.4       TCGGTGGACGACTTCCTGAAAGGCTCTGAACTTGGCAAGCAGCCGCACTGTTGCCATGGA
                  ******************************************. * :**  *.. * *..

HsCD00083683      CCAGCTTTCTTGTACAAAGTGGT-------------------------------------
NM_015347.4       GACGAGTACCACACAGAGAGCAGCCGGGGGTCTGACCTCTCAGACATCATGGAGGAGGAC
                   ..*. *:* : :...*..  .                                      

HsCD00083683      ------------------------------------------------------------
NM_015347.4       GAGGAGGAGCTGTATTCTGAAATGCAGCTGGAAGATGGGGGAAGGAGGCGGCCCAGCGGC
                                                                              

HsCD00083683      ------------------------------------------------------------
NM_015347.4       ACGTCCCACAATGCCCTCAAGATTTTAGGGAACCCAGCCTCTGCAGGACGGGTGGATCAC
                                                                              

HsCD00083683      ------------------------------------------------------------
NM_015347.4       ATGGGCCGGAGGTTTCCCCGTGGCAGCGCTGGTCCTCAGAGGTCCCGGCCCGTGACAGTC
                                                                              

HsCD00083683      ------------------------------------------------------------
NM_015347.4       CCATCCATCGACGATTACGGGCGAGACCGCCTTTCTCCAGACTTCTATGAAGAGTCAGAA
                                                                              

HsCD00083683      ------------------------------------------------------------
NM_015347.4       ACTGACCCTGGTGCCGAAGAGCTCCCGGCCCGGATCTTTGTGGCTCTCTTTGACTACGAC
                                                                              

HsCD00083683      ------------------------------------------------------------
NM_015347.4       CCGCTCACCATGTCCCCAAACCCAGATGCTGCAGAGGAGGAGCTTCCCTTTAAAGAAGGC
                                                                              

HsCD00083683      ------------------------------------------------------------
NM_015347.4       CAGATCATCAAGGTTTATGGTGATAAAGACGCTGATGGATTCTACCGTGGGGAAACCTGT
                                                                              

HsCD00083683      ------------------------------------------------------------
NM_015347.4       GCCCGGCTTGGCCTTATTCCTTGTAACATGGTCTCTGAGATACAAGCAGATGATGAGGAG
                                                                              

HsCD00083683      ------------------------------------------------------------
NM_015347.4       ATGATGGATCAGCTTCTTAGACAGGGCTTTCTCCCTCTGAATACACCTGTGGAGAAAATA
                                                                              

HsCD00083683      ------------------------------------------------------------
NM_015347.4       GAGAGAAGCAGGAGAAGTGGCAGGCGTCATTCGGTATCGACGCGGAGAATGGTGGCCCTG
                                                                              

HsCD00083683      ------------------------------------------------------------
NM_015347.4       TATGACTACGACCCCAGAGAAAGCTCGCCCAACGTCGATGTCGAGGCCGAACTTACATTT
                                                                              

HsCD00083683      ------------------------------------------------------------
NM_015347.4       TGCACAGGAGATATTATTACAGTTTTTGGTGAAATTGATGAAGATGGATTTTATTATGGG
                                                                              

HsCD00083683      ------------------------------------------------------------
NM_015347.4       GAGCTGAATGGGCAGAAAGGCCTTGTGCCCTCAAACTTCTTGGAAGAAGTGCCTGATGAC
                                                                              

HsCD00083683      ------------------------------------------------------------
NM_015347.4       GTAGAAGTCTATCTTTCTGATGCTCCATCCCACTACTCTCAAGATACGCCAATGCGCTCA
                                                                              

HsCD00083683      ---------------------------------------
NM_015347.4       AAGGCAAAAAGGAAGAAGAGTGTTCATTTCACACCTTAA
                                                         

Protein

SMART domain : FN3 : Fibronectin type 3 domain
SMART domain : SH3 : Src homology 3 domains
Structural Annotation Wiki : TOPSAN
UniProt : O15034
HPRD : 17183

Clone

Cloning Information : 268810
HIP Master Clone ID : 200385
Original Clone ID : 1025.1.64_GO.34963

Experimental

TargetTrack - Experimental data : GO.34963

Organism


TAX_ID : 9606
Species Specific ID: 23504

Genome

Chromosome : 12
Map Location : 12q24.33
Ensembl : ENSG00000060709

Reagents

Labome : RIMBP2-antibody