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1110067D22Rik (Mus musculus) in pVP16 (His and MBP-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  Lgalsl
Gene Name:  lectin, galactoside binding-like
Sequence             
Original Clone ID: 1385.1.25_GO.49308
Keyword: None
Species: Mus musculus
Type: cDNA
Vector Name: pVP16               Format:  CLOSED
Source: Center for Eukaryotic Structural Genomics
Description: None
Comments: His8-MBP fusion prtotein with TEV protease cleavage site is produced; after cleavage the protein obtained will be the translated CDS with the addition of SER at the N-term
Mutation/
Discrepancy :
No / No
Publications: None
Authors: University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
MmCD00083727

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 593nts         Open reading frame : 28 to 567

ACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCGCGGGGTCGGTG
GCCGACAGCGACGCCGTGGTGAAACTTGATGATGGGCACTTAAACAACTCCTTGGGTTCT
CCAGTTCAAGCCGACGTGTACTTCCCACGACTGATAGTTCCATTTTGTGGGCACATTAAA
GGTGGCATGAGACCAGGCAAGAAGGTATTAGTGATGGGCATCGTAGATCTCAACCCCGAA
AGCTTTGCCATCAGCTTGACCTGTGGAGACTCTGAAGATCCTCCTGCCGATGTGGCAATT
GAACTCAAAGCTGTGTTCACAGACCGGCAGCTGCTCAGAAACTCTTGTATCTCAGGAGAA
AGAGGCGAGGAGCAGTCGGCAATCCCTTACTTTCCATTCATCCCAGACCAGCCGTTCAGG
GTGGAGATTCTTTGCGAGCACCCACGTTTCAGAGTGTTTGTGGATGGACACCAACTTTTT
GATTTTTATCACCGTATTCAAACATTATCTGCAATTGACACCATAAAGATCAATGGGGAC
CTCCAGATCACCAAACTTGGCTGATAGGACCCAGCTTTCTTGTACAAAGTGGT


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 540
Start on reference sequence 1
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ACAAGTTTGTACAAAAAAGCAGGCTCC

3' Linker Sequence:
TAGGACCCAGCTTTCTTGTACAAAGTGGT

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pVP16

pVP16 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  MBP Forward
Reverse:  pQE Reverse
Description: Bacterial (E. coli) expression, adds N-terminal 8xHis and MBP, adds TEV site on 5p end of insert; ampicillin resistance in bacteria; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette.
Comments: TEV protease cleavage site is "partial" and not functional. The TEV cleavage site must be added to the insert by PCR, which allows amino acids encoded by the attB1 site to be cut off after protein expression. Gateway cloning into this vector will remove the Gateway cassette
Size (bp): 7631
Parent Vector: None
Empty Vector: EvNO00084279
Properties: Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name: Center for Eukaryotic Structural Genomics
Publications: PMID: 15750721
Title: Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type bacterial origin of replication 6471 5789
negative selection marker ccdB ccdB death cassette gene (lost in with-insert form) 2643 2852
promoter T5 T5 promoter 5 54
recombination site AttR1 AttR1 recombination site 1316 1440
recombination site AttR2 AttR2 recombination site 3020 2896
repressor protein gene LacI LacI coding region 4132 5211
selectable marker AmpR ampicillin resistance gene 7228 6569
selectable marker CmR chloramphenicol resistance gene (CmR) (Lost in with-insert form) 1549 2205
tag His N-terminal 8xHis tag 128 145
tag MBP N-terminal MBP 148 1246

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : Lgalsl
Symbol Nomenclature : Lgalsl
SYNONYM : 1110067D22Rik; A530071M23; Grpa; Hspc159; Lgalsla
Designation : galectin-related protein|galectin-related protein A|lectin galactoside-binding-like protein A
Full Nomenclature : lectin, galactoside binding-like
GENEID : 216551
Locus Tag : RP23-455B19.1
Mouse Genome Informatics : 1916114
Vega : OTTMUSG00000005233
Target GenBank: BC019131

 

Protein

SMART domain : GLECT : Galectin
SMART domain : Gal-bind_lectin : Galactoside-binding lectin
Structural Annotation Wiki : TOPSAN
UniProt : Q8VED9

Clone

Cloning Information : 268860
HIP Master Clone ID : 200435
Original Clone ID : 1385.1.25_GO.49308

Experimental

TargetTrack - Experimental data : GO.49308

Organism


TAX_ID : 10090
Species Specific ID: 216551

Genome

Chromosome : 11
Map Location : 11 A3.1|11 13.53 cM
Ensembl : ENSMUSG00000042363

Reagents

Labome : Lgalsl-antibody