Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Gene synthesis by Gen9. Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00840322
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 5886nts
Open reading frame: 1 to 5886
ATGTTTCGACAGTTTTATCTCTGGACATGTTTAGCTTCAGGGATCATCCTGGGCTCTCTC
TTTGAAATCTGCTTGGGCCAGTATGATGATGACTGGCAATATGAGGATTGCAAACTAGCT
AGGGGAGGACCACCAGCTACCATAGTTGCTATTGATGAAGAAAGTCGGAATGGTACAATT
CTGGTGGACAACATGCTGATCAAAGGGACTGCTGGAGGACCAGACCCCACCATAGAACTT
TCTTTAAAGGATAATGTGGATTACTGGGTGTTGATGGATCCTGTTAAGCAAATGCTTTTC
CTGAACAGCACCGGAAGAGTTCTGGATAGAGATCCACCGATGAACATACACTCCATTGTG
GTGCAGGTCCAGTGCATCAACAAAAAAGTGGGCACTATTATCTACCATGAAGTGCGAATA
GTGGTGAGAGACAGGAATGACAACTCACCCACTTTCAAGCATGAAAGCTACTATGCCACA
GTGAATGAGCTCACTCCAGTTGGTACCACAATATTCACAGGATTTTCAGGAGACAATGGA
GCTACAGATATAGATGATGGACCAAATGGACAGATAGAGTATGTTATTCAGTATAATCCA
GATGATCCGACATCCAATGACACCTTTGAAATTCCCCTAATGTTGACTGGAAATATAGTG
TTAAGGAAGAGGCTCAACTATGAAGATAAGACTCGCTACTTTGTCATAATCCAAGCTAAT
GACCGTGCCCAAAATCTGAATGAGAGGCGAACCACCACCACCACTCTCACAGTGGATGTT
CTGGATGGAGATGACTTGGGTCCAATGTTTCTTCCTTGTGTCCTTGTGCCAAACACTCGT
GATTGCCGTCCACTCACTTATCAAGCTGCCATACCTGAGTTGAGAACTCCGGAAGAACTG
AACCCCATTATTGTTACGCCACCAATCCAAGCCATTGATCAGGACCGGAATATTCAACCG
CCATCAGATAGGCCAGGAATCCTCTATTCCATCCTTGTTGGGACTCCTGAGGATTACCCA
CGATTTTTCCATATGCATCCTAGGACAGCAGAACTTAGTCTCCTGGAGCCAGTAAACAGA
GACTTTCACCAGAAATTTGATTTGGTTATTAAGGCTGAACAAGACAATGGTCATCCTCTT
CCTGCCTTTGCCGGTCTACACATTGAAATACTGGATGAAAACAATCAAAGTCCATATTTT
ACAATGCCCAGTTATCAAGGCTATATCCTGGAATCTGCCCCAGTGGGAGCAACCATTTCG
GACAGTCTCAATTTGACTTCACCTTTAAGAATAGTAGCTCTGGACAAGGACATAGAAGAT
ACAAAAGACCCAGAGCTTCACCTTTTTCTGAATGACTACACCTCAGTCTTCACCGTCACA
CAGACTGGTATTACTCGCTACCTCACCTTACTTCAACCAGTGGACAGGGAAGAACAGCAA
ACTTACACCTTTTCGATAACAGCATTTGATGGTGTACAAGAAAGTGAGCCAGTCATCGTC
AATATTCAAGTGATGGATGCAAATGATAACACGCCAACCTTCCCTGAAATATCCTATGAT
GTGTATGTTTATACAGACATGCGACCTGGGGACAGTGTCATACAGCTCACTGCAGTCGAC
GCAGACGAAGGGTCAAATGGGGAGATCACATATGAAATCCTTGTTGGGGCTCAGGGAGAC
TTCATCATCAATAAAACAACAGGGCTTATCACCATCGCTCCAGGGGTGGAAATGATAGTC
GGGCGGACTTACGCACTCACGGTCCAAGCAGCGGATAATGCTCCTCCTGCAGAGCGAAGG
AACTCCATCTGCACTGTGTATATTGAAGTGCTTCCACCAAATAATCAAAGCCCTCCTCGC
TTCCCACAGCTGATGTATAGCCTTGAAATTAGTGAAGCCATGAGGGTTGGTGCTGTTTTA
TTAAATCTACAGGCAACTGATCGAGAGGGAGACTCAATAACATATGCCATTGAGAATGGA
GATCCTCAGAGAGTTTTTAATCTTTCAGAAACCACGGGGATTCTAACCTTAGGGAAAGCA
CTGGACAGGGAAAGCACTGATCGCTACATTCTGATCATCACAGCTTCAGATGGCAGGCCA
GATGGGACCTCAACTGCCACAGTAAACATAGTGGTGACAGATGTCAATGACAATGCTCCA
GTGTTTGATCCTTATCTGCCAAGAAATTTATCTGTGGTGGAAGAAGAAGCCAATGCCTTT
GTGGGTCAAGTAAAAGCAACAGACCCTGATGCTGGAATAAATGGTCAAGTGCACTACAGT
TTGGGTAACTTTAATAATCTTTTTCGTATCACATCCAATGGGAGCATTTACACAGCAGTG
AAGCTTAACAGAGAAGTCAGGGACTACTATGAACTTGTTGTTGTGGCAACAGATGGAGCA
GTACACCCTCGTCATTCAACTCTAACCTTGGCCATCAAGGTTTTGGACATTGATGATAAC
AGTCCTGTGTTCACCAATTCAACATACACTGTCCTTGTTGAAGAGAATTTGCCAGCTGGG
ACTACCATCCTTCAAATAGAGGCCAAAGATGTCGACCTTGGAGCAAATGTGTCTTACCGG
ATAAGAAGCCCAGAAGTGAAGCACTTTTTTGCACTACATCCATTTACAGGAGAACTATCG
CTTTTAAGGAGTTTAGATTATGAGGCATTTCCAGACCAAGAAGCAAGTATCACTTTTCTG
GTAGAGGCCTTTGATATTTATGGAACAATGCCACCTGGTATTGCTACTGTCACAGTGATT
GTAAAGGATATGAATGATTATCCTCCTGTCTTTAGTAAACGAATATACAAAGGGATGGTG
GCTCCGGATGCAGTCAAGGGTACACCTATCACAACAGTTTATGCTGAAGATGCAGACCCT
CCTGGATTACCTGCAAGTCGTGTGAGGTATAGAGTAGATGATGTACAGTTTCCTTACCCT
GCCAGTATTTTTGAAGTGGAAGAAGATTCTGGAAGAGTAATAACACGAGTCAATCTTAAT
GAAGAACCTACAACAATTTTTAAGTTGGTGGTGGTTGCTTTTGATGATGGGGAGCCTGTG
ATGTCCAGCAGTGCCACAGTGAAGATTCTTGTCTTACATCCTGGTGAGATCCCACGCTTC
ACACAGGAGGAATATAGACCTCCTCCAGTAAGTGAACTTGCCACCAAAGGGACCATGGTT
GGTGTAATTTCTGCTGCTGCCATTAATCAAAGTATTGTGTACTCCATTGTTTCAGGAAAT
GAAGAAGATACATTTGGAATTAATAACATCACAGGTGTTATCTATGTGAATGGACCTCTG
GATTATGAAACCAGGACAAGCTATGTACTTCGAGTCCAAGCTGATTCCCTGGAAGTGGTC
CTTGCCAATCTCCGAGTTCCTTCAAAAAGCAATACAGCTAAAGTATACATTGAGATTCAG
GATGAAAATAATCATCCCCCAGTGTTTCAGAAAAAATTCTACATCGGAGGTGTATCTGAA
GATGCAAGAATGTTTACTTCTGTACTCAGAGTGAAGGCTACTGATAAAGATACTGGCAAT
TATAGTGTCATGGCCTACAGACTCATAATACCACCAATTAAAGAGGGAAAAGAAGGATTT
GTAGTGGAAACATATACAGGGCTTATCAAAACTGCTATGCTCTTCCATAATATGAGGAGA
TCCTACTTCAAGTTTCAAGTTATTGCAACTGACGACTATGGGAAGGGACTGAGCGGCAAA
GCCGATGTACTCGTCTCCGTGGTCAATCAGCTGGATATGCAAGTCATTGTTTCCAATGTG
CCTCCTACTCTAGTGGAAAAAAAGATAGAAGATCTTACAGAGATCTTGGATCGCTATGTT
CAGGAACAAATTCCTGGTGCCAAGGTCGTAGTGGAGTCCATTGGAGCTCGCCGGCATGGA
GATGCCTTTTCCCTAGAAGATTACACCAAATGTGACTTGACTGTCTATGCAATTGACCCC
CAAACCAACAGAGCCATCGATAGAAATGAGCTTTTTAAATTTTTGGATGGCAAACTACTT
GATATCAATAAAGACTTTCAGCCGTATTATGGGGAAGGAGGACGCATTCTGGAGATCCGG
ACTCCAGAGGCAGTGACCAGCATTAAAAAGAGAGGAGAAAGTCTAGGATACACAGAAGGG
GCCTTGTTGGCTCTGGCCTTCATCATCATCCTCTGCTGCATTCCTGCCATCTTGGTGGTT
TTGGTCAGCTACAGACAGTTTAAAGTACGTCAAGCTGAGTGTACAAAGACTGCACGAATT
CAGGCCGCATTACCCGCGGCTAAACCAGCAGTGCCGGCTCCTGCACCAGTGGCAGCGCCC
CCGCCGCCGCCGCCGCCTCCGCCAGGTGCGCATCTCTATGAAGAACTTGGAGACAGCTCA
ATGCATAATCTTTTCCTTCTCTACCATTTTCAACAAAGCAGGGGAAATAACTCAGTCTCA
GAAGACAGGAAACATCAACAAGTTGTGATGCCCTTTTCTTCCAATACTATTGAGGCTCAC
AAGTCAGCTCATGTAGACGGATCACTTAAGAGCAACAAACTGAAGTCTGCAAGAAAATTC
ACATTTCTATCTGATGAGGATGACTTAAGTGCCCATAATCCCCTTTATAAGGAAAACATA
AGTCAAGTATCAACAAATTCAGACATTTCACAGAGAACAGATTTTGTAGACCCATTTTCA
CCCAAAATACAAGCCAAGAGTAAGTCTCTGAGGGGCCCAAGAGAAAAGATTCAGAGGCTG
TGGAGTCAGTCAGTCAGCTTACCCAGGAGGCTGATGAGGAAAGTTCCAAATAGACCAGAG
ATCATAGATCTGCAGCAGTGGCAAGGCACCAGGCAGAAAGCTGAAAATGAAAACACTGGA
ATCTGTACAAACAAAAGAGGTAGCAGCAATCCATTGCTTACAACTGAAGAGGCAAATTTG
ACAGAGAAAGAGGAAATAAGGCAAGGTGAAACACTGATGATAGAAGGAACAGAACAGTTG
AAATCTCTCTCTTCAGACTCTTCATTTTGCTTTCCCAGGCCTCACTTCTCATTCTCCACT
TTGCCAACTGTTTCAAGAACTGTGGAACTCAAATCAGAACCTAATGTCATCAGTTCTCCT
GCTGAGTGTTCCTTGGAACTTTCTCCTTCAAGGCCTTGTGTTTTACATTCTTCACTCTCT
AGGAGAGAGACACCTATTTGTATGTTACCTATTGAAACCGAAAGAAATATTTTTGAAAAT
TTTGCCCATCCACCAAACATCTCTCCTTCTGCCTGTCCCCTTCCCCCTCCTCCTCCTATT
TCTCCTCCTTCTCCTCCTCCTGCTCCTGCTCCTCTTGCTCCTCCTCCTGACATTTCTCCT
TTTTCTCTTTTTTGTCCTCCTCCCTCTCCTCCTTCTATCCCTCTTCCTCTTCCTCCTCCT
ACATTTTTTCCACTTTCCGTTTCAACGTCTGGTCCCCCAACACCACCTCTTCTACCTCCA
TTTCCAACTCCTCTTCCTCCACCACCTCCTTCTATTCCTTGCCCTCCACCTCCTTCAGCT
TCATTTCTGTCCACAGAGTGTGTCTGTATAACAGGTGTTAAATGCACGACCAACTTGATG
CCTGCCGAGAAAATTAAGTCCTCTATGACACAGCTATCAACAACGACAGTGTGTAAAACA
GACCCTCAGAGAGAACCAAAAGGCATCCTCAGACACGTTAAAAACTTAGCAGAACTTGAA
AAATCAGTAGCTAACATGTACAGTCAAATAGAAAAAAACTATCTACGCACAAATGTTTCA
GAACTTCAAACTATGTGCCCTTCAGAAGTAACAAATATGGAAATCACATCTGAACAAAAC
AAGGGGAGTTTGAACAATATTGTCGAGGGAACTGAAAAACAATCTCACAGTCAATCTACT
TCACTG
Protein sequence predicted based on nucleotide sequence
MFRQFYLWTCLASGIILGSLFEICLGQYDDDWQYEDCKLARGGPPATIVAIDEESRNGTI LVDNMLIKGTAGGPDPTIELSLKDNVDYWVLMDPVKQMLFLNSTGRVLDRDPPMNIHSIV VQVQCINKKVGTIIYHEVRIVVRDRNDNSPTFKHESYYATVNELTPVGTTIFTGFSGDNG ATDIDDGPNGQIEYVIQYNPDDPTSNDTFEIPLMLTGNIVLRKRLNYEDKTRYFVIIQAN DRAQNLNERRTTTTTLTVDVLDGDDLGPMFLPCVLVPNTRDCRPLTYQAAIPELRTPEEL NPIIVTPPIQAIDQDRNIQPPSDRPGILYSILVGTPEDYPRFFHMHPRTAELSLLEPVNR DFHQKFDLVIKAEQDNGHPLPAFAGLHIEILDENNQSPYFTMPSYQGYILESAPVGATIS DSLNLTSPLRIVALDKDIEDTKDPELHLFLNDYTSVFTVTQTGITRYLTLLQPVDREEQQ TYTFSITAFDGVQESEPVIVNIQVMDANDNTPTFPEISYDVYVYTDMRPGDSVIQLTAVD ADEGSNGEITYEILVGAQGDFIINKTTGLITIAPGVEMIVGRTYALTVQAADNAPPAERR NSICTVYIEVLPPNNQSPPRFPQLMYSLEISEAMRVGAVLLNLQATDREGDSITYAIENG DPQRVFNLSETTGILTLGKALDRESTDRYILIITASDGRPDGTSTATVNIVVTDVNDNAP VFDPYLPRNLSVVEEEANAFVGQVKATDPDAGINGQVHYSLGNFNNLFRITSNGSIYTAV KLNREVRDYYELVVVATDGAVHPRHSTLTLAIKVLDIDDNSPVFTNSTYTVLVEENLPAG TTILQIEAKDVDLGANVSYRIRSPEVKHFFALHPFTGELSLLRSLDYEAFPDQEASITFL VEAFDIYGTMPPGIATVTVIVKDMNDYPPVFSKRIYKGMVAPDAVKGTPITTVYAEDADP PGLPASRVRYRVDDVQFPYPASIFEVEEDSGRVITRVNLNEEPTTIFKLVVVAFDDGEPV MSSSATVKILVLHPGEIPRFTQEEYRPPPVSELATKGTMVGVISAAAINQSIVYSIVSGN EEDTFGINNITGVIYVNGPLDYETRTSYVLRVQADSLEVVLANLRVPSKSNTAKVYIEIQ DENNHPPVFQKKFYIGGVSEDARMFTSVLRVKATDKDTGNYSVMAYRLIIPPIKEGKEGF VVETYTGLIKTAMLFHNMRRSYFKFQVIATDDYGKGLSGKADVLVSVVNQLDMQVIVSNV PPTLVEKKIEDLTEILDRYVQEQIPGAKVVVESIGARRHGDAFSLEDYTKCDLTVYAIDP QTNRAIDRNELFKFLDGKLLDINKDFQPYYGEGGRILEIRTPEAVTSIKKRGESLGYTEG ALLALAFIIILCCIPAILVVLVSYRQFKVRQAECTKTARIQAALPAAKPAVPAPAPVAAP PPPPPPPPGAHLYEELGDSSMHNLFLLYHFQQSRGNNSVSEDRKHQQVVMPFSSNTIEAH KSAHVDGSLKSNKLKSARKFTFLSDEDDLSAHNPLYKENISQVSTNSDISQRTDFVDPFS PKIQAKSKSLRGPREKIQRLWSQSVSLPRRLMRKVPNRPEIIDLQQWQGTRQKAENENTG ICTNKRGSSNPLLTTEEANLTEKEEIRQGETLMIEGTEQLKSLSSDSSFCFPRPHFSFST LPTVSRTVELKSEPNVISSPAECSLELSPSRPCVLHSSLSRRETPICMLPIETERNIFEN FAHPPNISPSACPLPPPPPISPPSPPPAPAPLAPPPDISPFSLFCPPPSPPSIPLPLPPP TFFPLSVSTSGPPTPPLLPPFPTPLPPPPPSIPCPPPPSASFLSTECVCITGVKCTTNLM PAEKIKSSMTQLSTTTVCKTDPQREPKGILRHVKNLAELEKSVANMYSQIEKNYLRTNVS ELQTMCPSEVTNMEITSEQNKGSLNNIVEGTEKQSHSQSTSL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
