Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Gene synthesis by Gen9. Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00840399
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 4488nts
Open reading frame: 1 to 4488
ATGGCCCTGAGTAAAGGGCTTCGGCTTCTTGGGCGCCTGGGGGCCGAAGGAGATTGTAGC
GTGCTTCTTGAGGCGCGCGGCCGAGACGATTGCCTTCTGTTTGAGGCCGGCACGGTTGCC
ACGCTTGCTCCAGAAGAAAAGGAAGTCATTAAAGGACAGTATGGCAAGCTCACGGACGCG
TACGGCTGCCTGGGGGAGCTGAGGCTGAAATCAGGTGGCACGTCTCTCAGCTTCCTGGTG
TTGGTGACAGGCTGCACATCTGTGGGCAGAATTCCAGATGCTGAAATTTACAAAATCACT
GCCACTGACTTTTACCCTCTTCAGGAAGAGGCCAAGGAGGAGGAACGCCTCATAGCTTTG
AAGAAAATCCTCAGCTCGGGGGTGTTCTATTTCTCATGGCCAAACGATGGGTCCCGGTTT
GACCTGACCGTCCGCACGCAGAAGCAGGGGGATGACAGCTCTGAATGGGGGAACTCCTTC
TTCTGGAACCAGCTCTTGCACGTGCCTTTGAGGCAGCACCAGGTGAGCTGCTGTGACTGG
CTGCTGAAGATCATCTGCGGGGTGGTCACCATCCGCACCGTCTATGCCTCACACAAGCAG
GCCAAGGCCTGCCTCGTCTCTCGCGTTAGCTGTGAGCGCACAGGCACTCGCTTCCACACC
CGTGGCGTGAACGACGACGGCCATGTGTCCAACTTCGTGGAGACAGAGCAGATGATTTAC
ATGGACGATGGAGTGTCATCTTTTGTCCAGATCAGAGGCTCCGTTCCGCTGTTCTGGGAA
CAGCCAGGGCTTCAGGTTGGCTCCCATCATCTGAGACTCCACAGAGGCCTGGAAGCCAAT
GCCCCTGCTTTCGACAGGCACATGGTGCTTCTGAAGGAGCAGTACGGGCAGCAAGTGGTC
GTGAACCTTCTGGGAAGCAGAGGCGGAGAGGAGGTGCTCAACAGAGCCTTCAAGAAGCTG
CTCTGGGCTTCCTGCCACGCGGGCGACACGCCCATGATCAATTTTGACTTCCATCAGTTT
GCCAAAGGTGGGAAGCTAGAGAAATTGGAAACCCTCTTGAGGCCACAGTTAAAGCTGCAC
TGGGAAGACTTCGATGTGTTCACAAAGGGGGAGAACGTCAGTCCACGTTTTCAGAAAGGC
ACTTTGCGGATGAACTGTCTGGACTGCCTGGACCGAACCAACACTGTGCAGAGCTTCATC
GCGCTGGAGGTCCTGCATCTGCAGCTCAAGACCCTGGGGCTGAGCTCAAAACCCATCGTT
GACCGCTTTGTGGAGTCCTTCAAAGCCATGTGGTCCCTGAATGGCCACAGCCTGAGCAAG
GTGTTCACAGGCAGCAGAGCCCTGGAAGGGAAGGCCAAGGTGGGGAAGCTGAAGGATGGA
GCCCGGTCCATGTCTCGGACCATCCAGTCCAACTTCTTCGACGGGGTGAAGCAGGAGGCC
ATCAAGCTGCTGCTGGTTGGGGACGTCTACGGCGAGGAGGTCGCAGACAAAGGGGGCATG
CTGCTGGACAGCACGGCGCTCCTGGTGACTCCTAGGATCCTGAAAGCTATGACTGAGCGT
CAGTCCGAATTCACAAATTTCAAGAGGATCCGGATTGCTATGGGGACCTGGAACGTGAAC
GGAGGAAAGCAGTTCCGGAGCAACGTGCTCAGGACGGCGGAGCTGACAGACTGGCTGCTC
GACTCGCCCCAGCTCTCGGGAGCTACCGACTCTCAGGATGACAGCAGCCCAGCTGACATA
TTTGCTGTGGGGTTTGAAGAGATGGTGGAATTGAGCGCAGGGAATATTGTCAATGCCAGT
ACTACCAACAAGAAGATGTGGGGTGAACAGCTTCAGAAAGCCATCTCACGCTCTCATAGA
TACATTCTGTTGACTTCGGCACAGCTGGTGGGCGTCTGTCTTTATATCTTTGTACGGCCA
TACCATGTCCCGTTCATCAGGGACGTAGCCATCGACACAGTGAAGACGGGCATGGGAGGC
AAGGCGGGGAACAAGGGCGCCGTCGGCATCCGCTTCCAGTTCCACAGCACCAGCTTCTGC
TTCATATGTAGTCACCTGACGGCCGGGCAGTCCCAGGTGAAGGAGCGGAATGAAGACTAC
AAGGAGATCACCCAGAAACTCTGCTTCCCAATGGGGAGAAATGTGTTTTCTCATGATTAT
GTATTTTGGTGTGGCGATTTCAACTACCGCATTGATCTTACTTATGAAGAAGTCTTCTAT
TTCGTTAAACGCCAAGACTGGAAGAAACTGCTGGAATTTGATCAACTACAGCTACAGAAA
TCAAGTGGAAAAATTTTTAAGGACTTTCACGAAGGAGCCATTAACTTTGGACCCACCTAC
AAGTATGACGTTGGCTCCGCCGCCTACGATACAAGCGACAAATGCCGCACCCCCGCCTGG
ACAGACAGGGTGCTGTGGTGGAGGAAGAAACATCCCTTTGATAAAACAGCTGGAGAACTG
AACCTTCTAGACAGTGATCTAGATGTTGACACCAAAGTCAGACACACCTGGTCCCCTGGT
GCCCTGCAGTATTATGGTCGTGCGGAGCTACAAGCGTCTGATCACAGACCTGTGCTGGCG
ATCGTGGAGGTGGAAGTTCAGGAAGTCGATGTGGGTGCTCGGGAGAGGGTTTTCCAGGAA
GTGTCCTCCTTCCAGGGCCCCCTGGATGCCACTGTTGTAGTAAACCTTCAATCACCGACC
TTAGAAGAGAAAAACGAGTTTCCAGAGGACCTGCGTACTGAGCTCATGCAGACCTTGGGG
AGCTATGGGACAATTGTTCTTGTCAGGATCAACCAAGGGCAGATGCTGGTAACTTTTGCA
GACAGTCACTCCGCTCTCAGTGTCCTGGACGTGGACGGTATGAAGGTGAAAGGCAGAGCA
GTGAAGATTAGACCGAAGACCAAGGACTGGCTGAAAGGTTTGCGAGAGGAGATCATTAGG
AAACGAGACAGCATGGCCCCCGTGTCTCCCACTGCCAACTCCTGTTTGCTGGAGGAAAAC
TTTGACTTCACAAGTTTGGACTATGAGTCCGAAGGGGATATTCTTGAAGACGATGAAGAC
TACTTGGTGGATGAATTCAATCAGCCTGGAGTCTCGGACAGTGAACTCGGGGGAGACGAC
CTCTCTGATGTCCCCGGCCCCACAGCACTGGCTCCTCCCAGCAAGTCCCCTGCTCTCACC
AAAAAGAAGCAGCATCCAACGTACAAAGATGACGCGGACCTGGTGGAGCTCAAGCGGGAG
CTCGAAGCCGTTGGGGAGTTCCGCCATCGTTCTCCGTCTAGGTCACTGTCTGTCCCCAAT
CGGCCTAGGCCACCTCAACCCCCGCAGAGGCCCCCTCCTCCAACCGGTTTAATGGTGAAA
AAGTCGGCTTCAGATGCGTCCATCTCCTCCGGCACCCATGGACAGTATTCAATTTTGCAG
ACGGCAAGGCTTCTACCAGGAGCACCTCAGCAACCTCCCAAGGCTCGGACTGGAATAAGT
AAACCTTATAATGTCAAGCAGATCAAAACCACCAATGCCCAGGAGGCAGAAGCAGCAATC
CGGTGTCTCCTGGAAGCCAGAGGAGGTGCCTCCGAAGAAGCCCTAAGTGCCGTGGCCCCA
CGGGACCTTGAAGCATCCTCTGAACCAGAGCCTACACCGGGGGCAGCTAAACCAGAGACA
CCACAAGCGCCCCCACTGCTTCCCCGTAGGCCCCCACCCAGAGTTCCTGCCATCAAGAAG
CCAACCTTGAGAAGGACAGGAAAGCCCCTGTCACCCGAAGAACAGTTTGAGCAACAGACT
GTCCATTTTACAATCGGGCCCCCGGAGACAAGCGTTGAGGCCCCTCCTGTCGTGACAGCC
CCTCGAGTCCCTCCTGTTCCCAAACCAAGAACATTTCAGCCTGGGAAAGCTGCAGAGCGG
CCAAGCCACAGGAAGCCAGCATCAGACGAAGCTCCTCCTGGAGCAGGAGCCTCTGTGCCA
CCACCTCTGGAGGCGCCGCCTCTTGTGCCTAAGGTACCCCCCAGAAGGAAGAAGTCAGCC
CCCGCAGCCTTCCACCTCCAGGTCCTGCAGAGCAACAGCCAGCTTCTCCAGGGCCTCACT
TACAATAGTAGTGACAGCCCCTCTGGGCACCCACCCGCCGCGGGCACAGTCTTCCCACAA
GGGGACTTTCTCAGCACTTCATCCGCTACAAGCCCCGACAGCGATGGCACCAAAGCGATG
AAGCCAGAGGCAGCCCCACTTCTTGGTGATTATCAGGACCCCTTCTGGAACCTTCTTCAC
CACCCTAAACTGTTGAATAACACTTGGCTTTCTAAGAGCTCAGACCCTTTGGACTCAGGA
ACCAGGAGCCCCAAAAGAGATCCCATAGACCCAGTGTCAGCTGGCGCTTCCGCTGCCAAG
GCAGAGCTGCCACCAGATCATGAACACAAAACCTTAGGTCACTGGGTGACAATCAGTGAC
CAAGAAAAGAGGACAGCACTGCAGGTCTTTGACCCACTGGCAAAAACA
Protein sequence predicted based on nucleotide sequence
MALSKGLRLLGRLGAEGDCSVLLEARGRDDCLLFEAGTVATLAPEEKEVIKGQYGKLTDA YGCLGELRLKSGGTSLSFLVLVTGCTSVGRIPDAEIYKITATDFYPLQEEAKEEERLIAL KKILSSGVFYFSWPNDGSRFDLTVRTQKQGDDSSEWGNSFFWNQLLHVPLRQHQVSCCDW LLKIICGVVTIRTVYASHKQAKACLVSRVSCERTGTRFHTRGVNDDGHVSNFVETEQMIY MDDGVSSFVQIRGSVPLFWEQPGLQVGSHHLRLHRGLEANAPAFDRHMVLLKEQYGQQVV VNLLGSRGGEEVLNRAFKKLLWASCHAGDTPMINFDFHQFAKGGKLEKLETLLRPQLKLH WEDFDVFTKGENVSPRFQKGTLRMNCLDCLDRTNTVQSFIALEVLHLQLKTLGLSSKPIV DRFVESFKAMWSLNGHSLSKVFTGSRALEGKAKVGKLKDGARSMSRTIQSNFFDGVKQEA IKLLLVGDVYGEEVADKGGMLLDSTALLVTPRILKAMTERQSEFTNFKRIRIAMGTWNVN GGKQFRSNVLRTAELTDWLLDSPQLSGATDSQDDSSPADIFAVGFEEMVELSAGNIVNAS TTNKKMWGEQLQKAISRSHRYILLTSAQLVGVCLYIFVRPYHVPFIRDVAIDTVKTGMGG KAGNKGAVGIRFQFHSTSFCFICSHLTAGQSQVKERNEDYKEITQKLCFPMGRNVFSHDY VFWCGDFNYRIDLTYEEVFYFVKRQDWKKLLEFDQLQLQKSSGKIFKDFHEGAINFGPTY KYDVGSAAYDTSDKCRTPAWTDRVLWWRKKHPFDKTAGELNLLDSDLDVDTKVRHTWSPG ALQYYGRAELQASDHRPVLAIVEVEVQEVDVGARERVFQEVSSFQGPLDATVVVNLQSPT LEEKNEFPEDLRTELMQTLGSYGTIVLVRINQGQMLVTFADSHSALSVLDVDGMKVKGRA VKIRPKTKDWLKGLREEIIRKRDSMAPVSPTANSCLLEENFDFTSLDYESEGDILEDDED YLVDEFNQPGVSDSELGGDDLSDVPGPTALAPPSKSPALTKKKQHPTYKDDADLVELKRE LEAVGEFRHRSPSRSLSVPNRPRPPQPPQRPPPPTGLMVKKSASDASISSGTHGQYSILQ TARLLPGAPQQPPKARTGISKPYNVKQIKTTNAQEAEAAIRCLLEARGGASEEALSAVAP RDLEASSEPEPTPGAAKPETPQAPPLLPRRPPPRVPAIKKPTLRRTGKPLSPEEQFEQQT VHFTIGPPETSVEAPPVVTAPRVPPVPKPRTFQPGKAAERPSHRKPASDEAPPGAGASVP PPLEAPPLVPKVPPRRKKSAPAAFHLQVLQSNSQLLQGLTYNSSDSPSGHPPAAGTVFPQ GDFLSTSSATSPDSDGTKAMKPEAAPLLGDYQDPFWNLLHHPKLLNNTWLSKSSDPLDSG TRSPKRDPIDPVSAGASAAKAELPPDHEHKTLGHWVTISDQEKRTALQVFDPLAKT
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
