Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00840771
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3261nts
Open reading frame: 1 to 3261
ATGACAACCGAATCTGGATCCGACTCGGAATCCAAGCCTGACCAAGAGGCTGAGCCCCAG
GAAGCTGCTGGGGCTCAAGGACGAGCTGGGGCTCCCGTTCCGGAGCCTCCCAAAGAAGAG
CAACAGCAGGCTTTAGAGCAGTTCGCCGCCGCTGCAGCTCACAGCACCCCTGTACGGAGG
GAGGTCACTGACAAGGAACAGGAGTTTGCTGCCAGGGCTGCAAAACAGCTCGAATATCAG
CAATTAGAAGACGATAAACTTTCTCAGAAATCATCTAGCAGTAAACTCTCTCGCTCTCCA
TTGAAGATTGTCAAAAAGCCTAAAAGCATGCAGTGCAAAGTGATACTTCTGGATGGATCA
GAATATACCTGTGATGTAGAGAAACGCTCCAGAGGCCAAGTGCTGTTTGATAAAGTGTGT
GAACACTTGAACCTGCTAGAGAAAGACTACTTTGGGCTTACGTATCGAGATGCTGAAAAC
CAGAAGAATTGGTTGGACCCTGCTAAGGAAATAAAAAAACAGGTTCGAAGTGGTGCTTGG
CACTTTTCATTTAATGTGAAATTTTATCCACCAGACCCTGCTCAACTATCTGAAGATATC
ACCAGGTACTACCTCTGCTTGCAGTTGCGAGATGACATCGTGTCCGGAAGGCTGCCCTGC
TCCTTTGTTACCCTGGCCTTGCTTGGCTCCTACACTGTCCAGTCAGAGCTGGGAGACTAT
GACCCAGATGAATGTGGGAGCGATTACATCAGTGAGTTCCGCTTTGCACCAAACCACACT
AAAGAACTGGAAGACAAAGTGATCGAGCTGCACAAGAGCCACAGAGGAATGACGCCAGCA
GAAGCAGAGATGCATTTCTTGGAAAATGCCAAAAAGTTATCAATGTATGGGGTAGATTTA
CATCATGCTAAGGACTCAGAAGGGGTAGAAATTATGTTAGGAGTTTGTGCAAGTGGTCTG
TTGATATATCGCGACCGGCTTAGAATAAACAGATTTGCCTGGCCCAAGGTTCTAAAGATT
TCATACAAACGGAACAACTTTTACATTAAGATCAGGCCCGGAGAGTTTGAACAATTTGAA
AGCACCATCGGGTTTAAGCTGCCAAACCATCGAGCTGCCAAGCGTTTATGGAAAGTATGT
GTGGAGCATCATACATTTTTCAGACTACTCTTACCAGAAGCACCTCCCAAGAAATTCCTA
ACCTTGGGTTCTAAGTTTCGTTATAGTGGCAGGACACAAGCGCAAACGAGAAGAGCCAGT
GCGTTGATAGATCGGCCCGCACCTTACTTTGAACGCTCATCTAGCAAACGTTATACCATG
TCTCGCAGCTTGGATGGAGAGGTTGGTACTGGCCAGTACGCCACAACCAAAGGGATCTCT
CAGACAAACTTGATCACCACCGTGACTCCGGAGAAGAAGGCTGAGGAGGAGCGGGACGAG
GAAGAGGACAAACGGAGGAAGGGGGAAGAAGTCACCCCCATCTCGGCCATCCGGCACGAG
GGAAAGTCACCTGGGCTTGGCACTGACTCCTGTCCCTTGTCACCCCCATCCACCCATTGT
GCCCCCACATCTCCCACAGAGCTCCGTAGGAGGTGTAAGGAGAATGACTGCAAACTGCCA
GGTTATGAGCCGTCCAGAGCTGAGCATCTGCCTGGAGAGCCCGCCTTGGACTCTGATGGC
CCAGGGAGGCCTTACCTAGGGGATCAAGATGTGGCTTTTAGCTACAGGCAGCAAACTGGC
AAGGGGACCACCCTGTTCTCCTTCTCCTTGCAGCTCCCTGAGTCATTCCCCTCCCTCCTA
GATGATGATGGATACCTCTCCTTCCCCAACCTTTCAGAAACCAACCTCCTGCCCCAGAGC
CTGCAGCATTACCTCCCGATCCGCTCACCGTCCCTTGTGCCCTGTTTCCTCTTCATCTTT
TTCTTTCTGCTGTCTGCCTCCTTCTCAGTGCCATACGCTCTCACTCTCTCCTTCCCTCTG
GCACTCTGCCTCTGCTACCTGGAACCCAAGGCCGCCTCCTTGAGCGCCTCCCTAGACAAT
GACCCGAGTGACAGTTCAGAGGAAGAGACTGACAGTGAGCGCACGGACACCGCAGCCGAC
GGGGAAACCACCGCTACTGAGTCCGACCAGGAGGAAGATGCAGAGCTCAAGGCACAGGAG
CTAGAAAAAACCCAAGATGACCTGATGAAACATCAAACCAACATTAGCGAGCTGAAAAGA
ACCTTCTTAGAAACATCAACAGACACTGCCGTAACGAATGAATGGGAGAAGAGGCTTTCC
ACCTCACCCGTGCGACTGGCCGCCAGGCAGGAGGATGCCCCCATGATCGAACCACTTGTC
CCAGAAGAGACTAAGCAGTCTTCCGGGGAAAAGCTCATGGATGGCTCTGAAATCTTCAGT
TTATTAGAGTCTGCGCGAAAACCAACAGAATTCATAGGAGGGGTTACTTCGACTTCTCAA
AGCTGGGTTCAGAAAATGGAAACCAAGACGGAGTCCAGCGGCATAGAGACGGAACCCACC
GTCCACCACCTCCCGCTTAGCACTGAGAAGGTGGTGCAGGAGACAGTGTTGGTGGAGGAG
AGGCGTGTGGTGCACGCGAGTGGGGATGCTTCTTACTCGGCGGGAGACAGCGGGGATGCT
GCAGCACAGCCCGCATTCACAGGCATTAAAGGGAAAGAGGGCTCTGCCTTGACGGAGGGG
GCTAAAGAGGAAGGAGGGGAGGAGGTCGCTAAAGCTGTCCTGGAACAGGAAGAGACAGCT
GCTGCTTCCCGTGAGCGACAAGAGGAGCAGAGTGCAGCCATCCACATTTCAGAAACTCTG
GAACAAAAACCTCATTTTGAGTCCTCAACGGTGAAGACCGAAACCATCAGCTTTGGATCT
GTTTCACCGGGAGGAGTAAAGCTGGAAATTTCCACGAAGGAAGTGCCCGTAGTCCACACC
GAAACCAAAACCATCACCTATGAATCATCACAGGTCGATCCAGGCACAGATCTGGAGCCA
GGCGTGCTGATGAGTGCACAGACGATCACATCTGAAACCACCAGTACCACCACCACTACG
CACATCACCAAAACTGTGAAAGGGGGCATTTCAGAGACAAGGATTGAGAAGCGAATAGTC
ATCACCGGGGATGCAGACATTGACCATGACCAGGCGCTGGCTCAGGCAATTAAAGAGGCC
AAAGAGCAGCACCCTGACATGTCCGTGACCAAAGTAGTCGTCCATAAAGAGACAGAGATC
ACACCAGAAGATGGAGAGGAT
Protein sequence predicted based on nucleotide sequence
MTTESGSDSESKPDQEAEPQEAAGAQGRAGAPVPEPPKEEQQQALEQFAAAAAHSTPVRR EVTDKEQEFAARAAKQLEYQQLEDDKLSQKSSSSKLSRSPLKIVKKPKSMQCKVILLDGS EYTCDVEKRSRGQVLFDKVCEHLNLLEKDYFGLTYRDAENQKNWLDPAKEIKKQVRSGAW HFSFNVKFYPPDPAQLSEDITRYYLCLQLRDDIVSGRLPCSFVTLALLGSYTVQSELGDY DPDECGSDYISEFRFAPNHTKELEDKVIELHKSHRGMTPAEAEMHFLENAKKLSMYGVDL HHAKDSEGVEIMLGVCASGLLIYRDRLRINRFAWPKVLKISYKRNNFYIKIRPGEFEQFE STIGFKLPNHRAAKRLWKVCVEHHTFFRLLLPEAPPKKFLTLGSKFRYSGRTQAQTRRAS ALIDRPAPYFERSSSKRYTMSRSLDGEVGTGQYATTKGISQTNLITTVTPEKKAEEERDE EEDKRRKGEEVTPISAIRHEGKSPGLGTDSCPLSPPSTHCAPTSPTELRRRCKENDCKLP GYEPSRAEHLPGEPALDSDGPGRPYLGDQDVAFSYRQQTGKGTTLFSFSLQLPESFPSLL DDDGYLSFPNLSETNLLPQSLQHYLPIRSPSLVPCFLFIFFFLLSASFSVPYALTLSFPL ALCLCYLEPKAASLSASLDNDPSDSSEEETDSERTDTAADGETTATESDQEEDAELKAQE LEKTQDDLMKHQTNISELKRTFLETSTDTAVTNEWEKRLSTSPVRLAARQEDAPMIEPLV PEETKQSSGEKLMDGSEIFSLLESARKPTEFIGGVTSTSQSWVQKMETKTESSGIETEPT VHHLPLSTEKVVQETVLVEERRVVHASGDASYSAGDSGDAAAQPAFTGIKGKEGSALTEG AKEEGGEEVAKAVLEQEETAAASRERQEEQSAAIHISETLEQKPHFESSTVKTETISFGS VSPGGVKLEISTKEVPVVHTETKTITYESSQVDPGTDLEPGVLMSAQTITSETTSTTTTT HITKTVKGGISETRIEKRIVITGDADIDHDQALAQAIKEAKEQHPDMSVTKVVVHKETEI TPEDGED
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
