DNASU Plasmid Repository • 480.965.5697 | Email

Detailed Vector Information: pMCSG20

TOP

Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG20              
Source: M. Donnelly
Description: Coexpression vector with T7 promoter, adds N-terminal Stag, GST, TEV, SNA; amp resistance; ligation independent cloning (LIC
Comments: None
Publications: PMID: 17363272
Title: New vectors for co-expression of proteins: structure of Bacillus subtilis ScoAB obtained by high-throughput protocols
PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.
Authors: M.Donnelly
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI
EvNO00084266

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pMCSG20

pMCSG20 in advanced viewed

Synonyms: pMCSG-20
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Coexpression vector with T7 promoter, adds N-terminal Stag, GST, TEV, SNA; amp resistance; ligation independent cloning (LIC
Comments: pMCSG7 derivative
Size (bp): 5991
Parent Vector: None
Properties: bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: PMID: 17363272
Title: New vectors for co-expression of proteins: structure of Bacillus subtilis ScoAB obtained by high-throughput protocols

PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS LIC Ligation independent cloning (LIC) site 204 235
bacterial origin ori ColE1-type bacterial origin of replication 3985 3985
promoter T7 T7 promoter 1065 1081
protease cleavage site TEV TEV cleavage site cds 223 243
repressor binding site lac operator LacO fragment 1040 1062
repressor protein gene LacI LacI coding sequence 1595 2554
selectable marker AmpR ampicillin resistance gene 4743 5603
tag S-tag N-terminal S-tag 946 990
tag GST Glutathione transferase (GST) tag 265 924
tag His C-terminal 6xHis tag 140 157
trxn termination sequence T7 term T7 terminator sequence 26 72

 

Other Link: SBKB Technology Portal
Other Link: SBKB Technology Portal
Other Link: MCSG Cloning Information
Other Link: MCSG Vector Information
Species Specific ID: None
Target GenBank: None