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Detailed Vector Information: pMCSG21

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG21              
Source: M. Donnelly
Description: Coexpression vector with T7 promoter, adds N-terminal His, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC).
Comments: None
Publications: PMID: 17363272
Title: New vectors for co-expression of proteins: structure of Bacillus subtilis ScoAB obtained by high-throughput protocols
PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.
Authors: M.Donnelly
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI
EvNO00084267

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AVAILABLE
No restriction
Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
       DyNA Vector Map

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Vector Name: pMCSG21

pMCSG21 in advanced viewed

Synonyms: pMCSG-21
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Coexpression vector with T7 promoter, adds N-terminal His, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC).
Comments: pCDFDuet-1 derivative, 2nd region replaced with LIC region
Size (bp): 3852
Parent Vector: None
Properties: bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: PMID: 17363272
Title: New vectors for co-expression of proteins: structure of Bacillus subtilis ScoAB obtained by high-throughput protocols

PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS LIC ligation-independent cloning region 353 384
MCS MCS MCS (BamHI, EcoRI, HindIII, AvaI) 385 430
MCS MCS MCS (BamHI, EcoRI, PstI, HindIII) 106 148
bacterial origin ori CloDF13-type bacterial origin of replication 1682 2420
promoter T7 T7 promoter 214 230
protease cleavage site TEV TEV cleavage site cds 345 365
repressor binding site lac operator lacO seqeuence region 5 25
repressor binding site lac operator 2 lacO sequence region 235 255
repressor protein gene LacI lacI cds 2630 3589
ribosome binding site RBS2 ribosome binding site 286 291
ribosome binding site RBS 3 Ribosome binding site 1548 1552
ribosome binding site RBS ribosome binding site 58 63
selectable marker SpectR spectinomycin resistance gene aadA 751 1542
tag His 2 6xHis tag 303 320
tag His 6xHis tag 83 100

 

Other Link: SBKB Technology Portal
Other Link: SBKB Technology Portal
Other Link: MCSG Cloning Information
Other Link: MCSG Vector Information
Species Specific ID: None
Target GenBank: None