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Detailed Vector Information: pMCSG8

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG8              
Source: M. Donnelly
Description: Vector facillitating reduced protein toxicity, with T7 promoter, adds N-terminal His tag, Sloop, TEV, SNA; amp resistance; ligation independent cloning (LIC).
Comments: Incorrect clone. Waiting for replacement clone
Publications: PMID: 11483004
Title: Expression of a highly toxic protein, Bax, in Escherichia coli by attachment of a leader peptide derived from the GroES cochaperone
PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.
Authors: M.Donnelly
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI
EvNO00084272

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AVAILABLE
No restriction
Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pMCSG8

pMCSG8 in advanced viewed

Synonyms: pMCSG-8
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Vector facillitating reduced protein toxicity, with T7 promoter, adds N-terminal His tag, Sloop, TEV, SNA; amp resistance; ligation independent cloning (LIC).
Comments: pMCSG7 derivative; Sloop = loop of GroES that binds GroEL
Size (bp): 5340
Parent Vector: None
Properties: bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: PMID: 11483004
Title: Expression of a highly toxic protein, Bax, in Escherichia coli by attachment of a leader peptide derived from the GroES cochaperone

PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS LIC Ligation independent cloning site 204 235
bacterial origin ori ColE1-type bacterial origin of replication 3997 3315
promoter T7 T7 promoter 414 430
protease cleavage site TEV TEV cleavage site cds 223 243
repressor binding site lac operator LacO fragment 389 411
repressor protein gene LacI LacI coding region 944 1903
selectable marker AmpR ampicillin resistance gene 4092 4952
tag His 2 C-terminal 6x His tag 140 157
tag S loop N-terminal S loop 250 302
tag His N-terminal 6x His tag 322 339
trxn termination sequence T7 term T7 terminator sequence 26 72

 

Species Specific ID: None
Target GenBank: None