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Detailed Vector Information: pMCSG9

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG9              
Source: M. Donnelly
Description: Vector with tags for solubility of insert, with T7 promoter, adds N-terminal His tag, MBP, TEV, SNA; amp resistance; ligation independent cloning (LIC).
Comments: None
Publications: PMID: 16497515
Title: An expression vector tailored for large-scale, high-throughput purification of recombinant proteins
Authors: M.Donnelly
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI
EvNO00084273

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Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
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Vector Name: pMCSG9

pMCSG9 in advanced viewed

Synonyms: pMCSG-9
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Vector with tags for solubility of insert, with T7 promoter, adds N-terminal His tag, MBP, TEV, SNA; amp resistance; ligation independent cloning (LIC).
Comments: pMCSG7 derivative
Size (bp): 6405
Parent Vector: None
Properties: bacterial expression, improves solubility of insert, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: PMID: 16497515
Title: An expression vector tailored for large-scale, high-throughput purification of recombinant proteins

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS LIC ligation-independent cloning (LIC) site 204 235
bacterial origin ori pBR322-type bacterial origin of replication 4399 4399
promoter T7 T7 promoter 1479 1495
protease cleavage site TEV TEV protease cleavage site cds 223 243
repressor binding site lac operator lacO regulatory sequence 1454 1476
repressor protein gene LacI LacI cds 2009 2968
selectable marker AmpR ampicillin resistance 5157 6017
tag His 2 N-terminal 6xHis tag 1387 1404
tag His 6xHis tag 140 157
tag MBP maltose binding protein cds 265 1362
trxn termination sequence T7 term T7 terminator 26 72

 

Other Link: SBKB Technology Portal
Other Link: MCSG Cloning Information
Other Link: MCSG Vector Information
Species Specific ID: None
Target GenBank: None