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Detailed Vector Information: pMCSG19C

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pMCSG19C              
Source: M. Donnelly
Description: Vector for solubility of insert and for purification, with T7 promoter, adds N-terminal MBP, TVMV, His, TEV, NA; amp resistance; ligation independent cloning (LIC).
Comments: None
Publications: None
Authors: M.Donnelly
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI
EvNO00084274

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AVAILABLE
No restriction
Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pMCSG19C

pMCSG19C in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Vector for solubility of insert and for purification, with T7 promoter, adds N-terminal MBP, TVMV, His, TEV, NA; amp resistance; ligation independent cloning (LIC).
Comments: as 19 + constitutive expression of TVMV protease from integrated gene
Size (bp): 7468
Parent Vector: None
Properties: in vitro transcription, ligation independent cloning (LIC)
Author Name: M.Donnelly
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 5432 5432
gene fragment TVMV cds TVMV protease gene 1927 2637
ligation independent cloning LIC LIC Site 204 235
promoter T7 T7 promoter 1515 1531
protease cleavage site TVMV TVMV protease cleavage site 286 306
protease cleavage site TEV protease cleavage site 223 243
repressor binding site lac operator LacO fragment 1490 1512
repressor binding site tet operator TetO fragment 1753 1908
repressor protein gene LacI LacI CDS 3072 4031
selectable marker AmpR ampicillin resistance gene 6220 7080
tag His N-terminal 6x His tag 268 285
tag MBP N-terminal MBP tag 337 1443
trxn termination sequence T7 term T7 terminator 26 72

 

Other Link: MCSG Cloning Information
Other Link: MCSG Vector Information
Species Specific ID: None
Target GenBank: None