Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00842788
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 4863nts
Open reading frame: 1 to 4863
ATGAATTCACAACCTCAAACCCGTTCTCCGTTTTTCCAGAGGCCTCAAATACAGCCTCCT
AGAGCTACCATCCCGAACAGCAGTCCTTCCATTCGTCCTGGTGCACAGACACCCACTGCA
GTGTACCAGGCTAATCAGCACATCATGATGGTTAACCATCTGCCCATGCCCTACCCAGTG
CCCCAGGGGCCTCAGTACTGTATACCACAGTACCGTCATAGTGGCCCTCCTTATGTGGGG
CCCCCCCAACAATATCCAGTTCAACCACCGGGGCCAGGTCCCTTTTATCCTGGACCAGGA
CCCGGGGACTTCCCCAATGCTTATGGAACGCCTTTTTACCCAAGTCAGCCGGTGTATCAG
TCAGCACCTATCATAGTGCCTACGCAGCAACAGCCGCCTCCCGCTAAGAGAGAGAAAAAA
ACTATAAGAATTCGGGATCCAAACCAGGGAGGTAAAGACATAACAGAGGAGATTATGTCT
GGAGGTGGCAGTAGAAATCCTACTCCACCCATAGGAAGACCCACGTCTACACCTACTCCT
CCTCAGCTGCCCAGCCAGGTGCCCGAGCACAGCCCTGTGGTTTATGGGACTGTGGAGAGC
GCTCATCTTGCTGCCAGTACCCCTGTCACTGCAGCTAGCGACCAGAAGCAAGAGGAGAAG
CCAAAACCAGATCCAGTGTTAAAGTCTCCTTCCCCAGTCCTTAGGCTAGTCCTCAGTGGA
GAGAAGAAAGAACAAGAAGGCCAGACATCTGAAACTACTGCAATAGTATCCATAGCAGAG
CTTCCTCTGCCACCATCACCTACAACTGTTTCTTCTGTTGCTCGAAGCACAATTGCAGCC
CCCACCTCTTCTGCTCTTAGTAGCCAACCAATATTCACCACTGCTATAGATGACAGATGT
GAACTCTCATCCCCAAGAGAAGACACAATTCCTATACCCAGCCTCACATCTTGCACCGAA
ACATCAGACCCTTTACCAACAAATGAAAATGATGATGATATATGCAAGAAACCCTGTAGT
GTAGCACCTAATGATATTCCACTGGTTTCTAGTACTAACCTAATTAATGAAATAAATGGA
GTTAGCGAAAAATTATCAGCCACGGAGAGCATTGTGGAAATAGTAAAACAGGAAGTACTG
CCATTGACTCTTGAACTGGAGATTCTGGAAAATCCCCCCGAAGAAATGAAACTGGAGTGT
ATCCCAGCTCCCATCACCCCTTCCACAGTTCCTTCCTTTCCTCCAACTCCTCCAACTCCT
CCAGCTTCTCCTCCTCACACTCCAGTCATTGTTCCTGCTGCTGCCACTACTGTGAGTTCT
CCGAGTGCTGCTATCACAGTCCAGAGAGTCCTAGAGGAGGACGAGAGCATAAGAACTTGC
CTTAGTGAAGATGCAAAAGAGATTCAGAACAAAATAGAGGTAGAAGCAGATGGGCAAACC
GAAGAGATTTTGGATTCTCAAAACTTAAATTCAAGAAGGAGCCCTGTCCCAGCTCAAATA
GCTATAACTGTACCAAAGACATGGAAGAAACCAAAAGATCGGACCCGAACCACTGAAGAG
ATGTTAGAGGCAGAATTGGAGCTGAAAGCTGAAGAGGAGCTTTCCATTGACAAAGTACTT
GAATCTGAACAAGATAAAATGAGCCAGGGGTTTCATCCTGAACGGGACCCCTCTGACCTA
AAAAAAGTGAAAGCTGTGGAAGAAAATGGAGAAGAAGCTGAGCCAGTACGTAATGGTGCT
GAGAGTGTTTCTGAGGGTGAAGGAATCGATGCTAATTCAGGCTCCACAGATAGTTCTGGT
GATGGGGTTACATTTCCATTTAAACCAGAATCCTGGAAGCCTACCGATACTGAAGGTAAG
AAGCAGTATGACAGGGAGTTTCTGCTGGACTTCCAGTTCATGCCTGCCTGTATACAAAAA
CCAGAGGGCCTGCCTCCTATCAGTGATGTGGTTCTTGACAAGATCAACCAACCCAAATTG
CCAATGCGAACTCTGGATCCTCGAATTTTGCCTCGAGGACCCGACTTTACACCCGCCTTT
GCTGATTTTGGAAGGCAGACACCTGGTGGAAGAGGCGTACCTATTTGCAAAGTGCAGAGC
AGGCATGGATTGCCAATTCTGGAACAGAGCAAAGCCCCAACTTGCCCTCCACTCGTGATG
TCACACCCACCCATGAAGAGCCTGCCTCTAGGGTTGTTGAATGTTGGGTCACGAAGATCT
CAACCTGGCCAAAGAAGAGAACCCAGAAAGATCATTACAGTTTCTGTAAAAGAAGATGTA
CACCTGAAAAAGGCAGAAAATGCCTGGAAGCCAAGCCAAAAACGAGACTCCCAAGCCGAT
GATCCCGAAAACATTAAAACCCAGGAGCTTTTTAGAAAAGTTCGAAGTATCTTAAATAAA
CTGACACCACAGATGTTCAATCAACTGATGAAGCAAGTGTCAGGACTTACTGTTGACACA
GAGGAGCGGCTGAAAGGAGTTATTGACCTGGTCTTTGAGAAGGCTATTGATGAACCCAGT
TTCTCTGTGGCTTACGCCAACATGTGTCGATGTCTAGTAACGCTGAAAGTACCCATGGCA
GACAAGCCTGGTAACACAGTGAATTTCCGGAAGCTGCTACTGAACCGTTGCCAGAAGGAG
TTTGAAAAAGATAAAGCAGATGATGATGTCTTTGAGAAGAAGCAGAAAGAACTTGAGGCT
GCCAGTGCTCCAGAGGAGAGGACAAGGCTTCATGATGAACTGGAAGAAGCCAAGGACAAA
GCCCGGCGGAGATCTATTGGCAACATCAAGTTTATTGGAGAACTCTTTAAACTCAAAATG
CTCACTGAAGCCATCATGCATGACTGTGTGGTGAAGCTGCTAAAGAACCATGATGAAGAA
TCCCTCGAGTGCCTGTGTCGCCTGCTCACCACCATTGGCAAAGACTTGGACTTTGAAAAA
GCAAAGCCACGTATGGACCAGTACTTTAATCAGATGGAGAAAATTGTGAAAGAAAGAAAA
ACCTCATCTAGGATTCGGTTCATGCTTCAAGATGTTATAGACCTAAGGCTGTGCAATTGG
GTATCACGAAGGGCAGATCAAGGGCCTAAAACTATCGAACAGATTCACAAAGAGGCTAAA
ATAGAAGAACAAGAAGAGCAAAGGAAGGTCCAGCAACTCATGACCAAAGAGAAGAGAAGA
CCAGGTGTCCAGAGAGTGGACGAAGGTGGGTGGAACACTGTACAAGGGGCCAAGAACAGT
CGGGTACTGGACCCCTCAAAATTCCTAAAAATCACTAAGCCTACAATTGATGAAAAAATT
CAGCTGGTACCTAAAGCACAGCTAGGAAGCTGGGGAAAAGGCAGCAGTGGTGGAGCAAAG
GCAAGTGAGACTGATGCCTTACGGTCTAGTGCTTCCAGCTTAAACAGATTCTCTGCCCTG
CAACCTCCCGCACCCTCAGGGTCCACCCCATCCACGCCTGTAGAGTTTGATTCACGAAGG
ACCTTAACTAGTCGTGGAAGTATGGGCAGGGAGAAGAATGACAAGCCCCTTCCATCTGCA
ACCGCCCGGCCAAATACTTTCATGAGGGGTGGCAGCAGTAAAGACCTCCTAGACAATCAG
TCCCAAGAAGAGCAGCGGAGAGAGATGCTGGAAACCGTGAAGCAGCTCACAGGAGGTGTG
GATGTGGAGAGGAACAGCACTGAGGCTGAGCGAAATAAAACAAGGGAGTCAGCAAAACCA
GAAATTTCAGCAATGTCAGCCCATGACAAGGCAGCATTATCAGAAGAGGAACTGGAGAGG
AAGTCGAAATCTATCATTGATGAATTTCTACACATTAATGATTTTAAGGAAGCCATGCAG
TGTGTGGAAGAGCTGAATGCCCAGGGCCTGCTGCATGTTTTTGTGAGAGTGGGAGTGGAG
TCCACCCTGGAAAGAAGCCAGATCACCAGGGATCACATGGGCCAATTACTCTATCAGCTG
GTACAGTCAGAAAAACTGAGCAAACAGGACTTTTTCAAAGGTTTTTCAGAAACTTTGGAA
TTGGCAGATGACATGGCCATTGATATTCCCCATATTTGGTTGTACCTCGCTGAACTGGTG
ACCCCCATGTTAAAAGAAGGTGGAATCTCCATGAGGGAACTTACCATAGAATTTAGCAAA
CCTTTACTTCCTGTTGGAAGAGCTGGGGTCTTGCTATCCGAAATATTGCACCTACTATGC
AAACAAATGAGCCATAAGAAAGTGGGAGCCTTATGGAGGGAGGCTGACCTCAGCTGGAAG
GACTTTTTACCAGAAGGAGAAGATGTACATAATTTTCTTTTGGAGCAGAAGTTGGACTTC
ATAGAGTCTGACAGTCCCTGTTCCTCTGAAGCACTTTCAAAGAAAGAACTGTCTGCCGAA
GAGCTGTATAAGCGACTCGAGAAACTCATTATTGAGGACAAAGCGAATGATGAACAGATC
TTTGACTGGGTAGAGGCTAATCTAGACGAGATCCAGATGAGTTCCCCCACATTCCTTAGA
GCTTTAATGACTGCTGTTTGTAAAGCAGCTATTATAGCCGACTCTTCTACCTTCAGAGTC
GACACTGCTGTTATCAAACAGAGAGTGCCGATCTTACTCAAGTACCTAGACTCAGATACA
GAGAAGGAACTGCAAGCACTTTATGCACTACAAGCATCGATAGTAAAACTTGATCAACCT
GCCAATCTGCTGAGGATGTTTTTTGATTGTCTATATGACGAGGAGGTGATCTCCGAGGAT
GCCTTCTACAAATGGGAGAGCAGCAAGGACCCTGCAGAGCAGAATGGGAAGGGCGTGGCT
CTGAAATCTGTCACGGCATTCTTCACGTGGCTGCGGGAAGCAGAAGAGGAGTCTGAGGAT
AAC
Protein sequence predicted based on nucleotide sequence
MNSQPQTRSPFFQRPQIQPPRATIPNSSPSIRPGAQTPTAVYQANQHIMMVNHLPMPYPV PQGPQYCIPQYRHSGPPYVGPPQQYPVQPPGPGPFYPGPGPGDFPNAYGTPFYPSQPVYQ SAPIIVPTQQQPPPAKREKKTIRIRDPNQGGKDITEEIMSGGGSRNPTPPIGRPTSTPTP PQLPSQVPEHSPVVYGTVESAHLAASTPVTAASDQKQEEKPKPDPVLKSPSPVLRLVLSG EKKEQEGQTSETTAIVSIAELPLPPSPTTVSSVARSTIAAPTSSALSSQPIFTTAIDDRC ELSSPREDTIPIPSLTSCTETSDPLPTNENDDDICKKPCSVAPNDIPLVSSTNLINEING VSEKLSATESIVEIVKQEVLPLTLELEILENPPEEMKLECIPAPITPSTVPSFPPTPPTP PASPPHTPVIVPAAATTVSSPSAAITVQRVLEEDESIRTCLSEDAKEIQNKIEVEADGQT EEILDSQNLNSRRSPVPAQIAITVPKTWKKPKDRTRTTEEMLEAELELKAEEELSIDKVL ESEQDKMSQGFHPERDPSDLKKVKAVEENGEEAEPVRNGAESVSEGEGIDANSGSTDSSG DGVTFPFKPESWKPTDTEGKKQYDREFLLDFQFMPACIQKPEGLPPISDVVLDKINQPKL PMRTLDPRILPRGPDFTPAFADFGRQTPGGRGVPICKVQSRHGLPILEQSKAPTCPPLVM SHPPMKSLPLGLLNVGSRRSQPGQRREPRKIITVSVKEDVHLKKAENAWKPSQKRDSQAD DPENIKTQELFRKVRSILNKLTPQMFNQLMKQVSGLTVDTEERLKGVIDLVFEKAIDEPS FSVAYANMCRCLVTLKVPMADKPGNTVNFRKLLLNRCQKEFEKDKADDDVFEKKQKELEA ASAPEERTRLHDELEEAKDKARRRSIGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEE SLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLCNW VSRRADQGPKTIEQIHKEAKIEEQEEQRKVQQLMTKEKRRPGVQRVDEGGWNTVQGAKNS RVLDPSKFLKITKPTIDEKIQLVPKAQLGSWGKGSSGGAKASETDALRSSASSLNRFSAL QPPAPSGSTPSTPVEFDSRRTLTSRGSMGREKNDKPLPSATARPNTFMRGGSSKDLLDNQ SQEEQRREMLETVKQLTGGVDVERNSTEAERNKTRESAKPEISAMSAHDKAALSEEELER KSKSIIDEFLHINDFKEAMQCVEELNAQGLLHVFVRVGVESTLERSQITRDHMGQLLYQL VQSEKLSKQDFFKGFSETLELADDMAIDIPHIWLYLAELVTPMLKEGGISMRELTIEFSK PLLPVGRAGVLLSEILHLLCKQMSHKKVGALWREADLSWKDFLPEGEDVHNFLLEQKLDF IESDSPCSSEALSKKELSAEELYKRLEKLIIEDKANDEQIFDWVEANLDEIQMSSPTFLR ALMTAVCKAAIIADSSTFRVDTAVIKQRVPILLKYLDSDTEKELQALYALQASIVKLDQP ANLLRMFFDCLYDEEVISEDAFYKWESSKDPAEQNGKGVALKSVTAFFTWLREAEEESED N
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
