Bacterial (E. coli) expression, adds N-terminal 8xHis and MBP, adds TEV site on 5p end of insert; amp resistance; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette.
Comments:
None
Publications:
PMID:15750721 Title: Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination PMID:19365792 Title: Autoinduction of protein expression.
Authors:
University of Wisconsin
PSI
Center for Eukaryotic Structural Genomics
Bacterial (E. coli) expression, adds N-terminal 8xHis and MBP, adds TEV site on 5p end of insert; ampicillin resistance in bacteria; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette.
Comments:
TEV protease cleavage site is "partial" and not functional. The TEV cleavage site must be added to the insert by PCR, which allows amino acids encoded by the attB1 site to be cut off after protein expression. Gateway cloning into this vector will remove the Gateway cassette
Size (bp):
7631
Parent Vector:
None
Properties:
Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker