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Detailed Vector Information: pVP33A

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pVP33A              
Source: CESG
Description: Bacterial (E. coli) expression, adds N-terminal 8xHis, MBP, 3CP cleavage, tetra-cys, Tev cleavage; amp resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte.
Comments: None
Publications: PMID: 16377204
Title: High efficiency single step production of expression plasmids from cDNA clones using the Flexi Vector cloning system
Authors: PSI
Center for Eukaryotic Structural Genomics
University of Wisconsin
EvNO00084280

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AVAILABLE
No restriction
Special MTA: PSI,Promega

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : BR610
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pVP33A

pVP33A in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  MBP Forward
Reverse:  T7 Terminator
Description: Bacterial (E. coli) expression, adds N-terminal 8xHis, MBP, 3CP cleavage, tetra-cys, Tev cleavage; amp resistance; restriction enzyme cloning (SgfI, PmeI).
Comments: Cloning into this vector will remove the BarCat casstte
Size (bp): 7228
Parent Vector: None
Properties: bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name: Center for Eukaryotic Structural Genomics
Publications: PMID: 16377204
Title: High efficiency single step production of expression plasmids from cDNA clones using the Flexi Vector cloning system

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori pBR322-type bacterial origin of replication 5422 6104
gene fragment BarCat-cat CAT cds - chloramphenicol resistance 1819 2476
gene fragment BarCat-bar Barnase cds 1381 1717
primer site T7 rev T7 reverse primer site 2533 2551
promoter T5 pr 2 T5 promoter (two of two) 0 0
promoter T5 T5 promoter 43 89
protease cleavage site TEV TEV cleavage site cds 1335 1355
protease cleavage site 3C 3CP protease cleavage site cds 1293 1317
recombination site pF1K site pF1K homology site 2484 2612
repressor protein gene LacIq LacIq 3762 4841
selectable marker AmpR ampicillin resistance gene 6202 6861
tag His 8xHis epitope tag 157 180
tag tetraCys tetraCyc cds 1318 1335
tag MBP MBP fusion protein tag 187 1283

 

Other Link: SBKB Technology Portal
Species Specific ID: None
Target GenBank: None