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Detailed Vector Information: pVP56A

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pVP56A              
Source: CESG
Description: Bacterial (E. coli) expression, adds N-terminal 8xHis, MBP, 3CP cleavage, Tev cleavage; amp resistance; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat casstte.
Comments: None
Publications: PMID: 16377204
Title: High efficiency single step production of expression plasmids from cDNA clones using the Flexi Vector cloning system
Authors: Center for Eukaryotic Structural Genomics
PSI
University of Wisconsin
EvNO00084281

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AVAILABLE
No restriction
Special MTA: PSI,Promega

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : BR610
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pVP56A

pVP56A in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  MBP Forward
Reverse:  T7 Terminator
Description: Bacterial (E. coli) expression, adds N-terminal 8xHis, MBP, 3CP cleavage, Tev cleavage; amp resistance; restriction enzyme cloning (SgfI, PmeI).
Comments: Cloning into this vector will remove the BarCat casstte
Size (bp): 7222
Parent Vector: None
Properties: bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name: CESG
Publications: PMID: 16377204
Title: High efficiency single step production of expression plasmids from cDNA clones using the Flexi Vector cloning system

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type bacterial origin of replication 6098 5416
promoter T5 T5 promoter 43 89
protease cleavage site TEV TEV protease cleavage site 1333 1353
protease cleavage site 3C 3C protease cleavage site 1308 1332
repressor protein gene LacI LacI cds 3759 4838
selectable marker Barnase Barnase gene 1378 1714
selectable marker AmpR ampicillin resistance gene 6855 6196
selectable marker CmR chloramphenicol resistance gene (CmR) 1817 2473
tag MBP MBP tag 187 1283
tag His 6xHis tag 157 180

 

Other Link: SBKB Technology Portal
Species Specific ID: None
Target GenBank: None