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Detailed Vector Information: pPSU1

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pPSU1              
Source: Song Lab, Penn State University
Description: High copy number plasmid which provides 500, 1000, 1500, 2000 and 5000 bp EcoRV fragments of kilobase DNA ladder, and 500, 700, 800, 900, 1000, 2000 and 4100 bp PstI fragments of 100 bp DNA ladder. Confers ampicillin resistance.
Comments: Published in Nature Scientific Reports, this plasmid produces DNA molecular weight reference markers when digested with common restriction enzymes. Usually used with pPSU2.Map of the key restriction sites
Standard protocol for using this plasmid
Publications: PMID: 28550309
Title: The pPSU Plasmids for Generating DNA Molecular Weight Markers
Authors: Song Tan
EvNO00846203

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
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Vector Name: pPSU1

pPSU1 in advanced viewed

Synonyms: ''
Sequencing Primer: Forward:  None
Reverse:  None
Description: High copy number plasmid which provides 500, 1000, 1500, 2000 and 5000 bp EcoRV fragments of kilobase DNA ladder, and 500, 700, 800, 900, 1000, 2000 and 4100 bp PstI fragments of 100 bp DNA ladder. Confers ampicillin resistance.
Comments: Published in Nature Scientific Reports, this plasmid produces DNA molecular weight reference markers when digested with common restriction enzymes. Usually used with pPSU2. Map of the key restriction sites
Standard protocol for using this plasmid
Size (bp): 10000
Parent Vector: Name: pUC9
Description:
Properties: high copy number bacterial plasmid
Author Name: Song Tan
Publications: PMID: 28550309
Title: The pPSU Plasmids for Generating DNA Molecular Weight Markers

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
restriction site EcoRV 500bp EcoRV 500 bp Fragment 241 741
restriction site EcoRV 1kb EcoRV 1000 bp Fragment 741 1741
restriction site EcoRV 1.5kb EcoRV 1500 bp Fragment 1741 3241
restriction site EcoRV 2kb EcoRV 2000 bp Fragment 3241 5241
restriction site EcoRV 5kb EcoRV 5000 bp Fragment 5241 241
restriction site PstI 2kb PstI 2000 bp Fragment 2196 4196
restriction site PstI 700 bp PstI 700 bp Fragment 5196 5896
restriction site PstI 800 bp PstI 800 bp Fragment 5896 6696
restriction site PstI 900 bp PstI 900 bp Fragment 6696 7596
restriction site PstI 4100 bp PstI 4100 bp Fragment 7596 1696
restriction site PstI 500bp PstI 500 bp fragment 1696 2196
restriction site PstI 1kb PstI 1000 bp fragment 4196 5196

 

Species Specific ID: None
Target GenBank: None