TM0936 (T. maritima ) in pMH1 (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  TM0936
Gene Name:  hypothetical protein
Original Clone ID:
8173
Keyword:
None
Species:
Thermotoga maritima
Type:
cDNA
Vector Name:
pMH1
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
TmCD00084735
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1221nts
Open reading frame: 51 to 1271

AGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACATGATCATAG
GAAATTGCCTGATACTGAAAGATTTCTCTTCTGAACCTTTCTGGGGAGCCGTAGAGATCG
AAAACGGCACGATAAAACGAGTGTTACAGGGAGAAGTGAAGGTCGATCTGGATCTCTCCG
GCAAACTCGTCATGCCGGCTCTTTTCAACACGCACACTCACGCGCCGATGACTCTTCTGA
GAGGAGTCGCGGAAGATCTCAGTTTCGAAGAGTGGCTCTTCTCTAAGGTGCTTCCCATAG
AAGACAGATTGACAGAGAAGATGGCCTACTACGGTACGATCCTTGCCCAGATGGAAATGG
CAAGACATGGTATCGCCGGCTTTGTCGATATGTACTTTCATGAGGAGTGGATTGCAAAGG
CAGTCAGAGATTTTGGAATGAGAGCCCTCTTGACACGGGGACTTGTGGACAGTAATGGGG
ACGATGGAGGACGACTCGAGGAGAATTTGAAACTCTACAACGAGTGGAACGGTTTTGAGG
GGAGGATTTTCGTTGGATTCGGTCCTCACTCACCTTACCTTTGTTCTGAAGAATACCTGA
AGAGAGTCTTCGACACTGCGAAGTCTCTGAACGCTCCAGTGACGATTCATCTCTATGAAA
CCTCCAAAGAAGAGTATGATCTGGAGGACATTTTGAACATCGGGTTGAAAGAAGTGAAGA
CCATCGCAGCTCATTGTGTTCATCTTCCCGAGAGATATTTCGGTGTTCTGAAAGATATTC
CGTTTTTCGTTTCTCACAATCCTGCGAGCAATTTGAAACTCGGAAACGGAATAGCACCTG
TTCAGAGAATGATAGAGCATGGGATGAAGGTCACCCTCGGCACGGATGGAGCGGCGAGCA
ACAACTCTCTGAATCTGTTCTTCGAAATGAGGCTCGCGAGTCTTCTTCAGAAAGCACAGA
ATCCGCGCAATCTGGATGTGAACACATGCTTGAAAATGGTGACGTACGATGGAGCCCAAG
CGATGGGGTTCAAAAGCGGAAAGATTGAAGAAGGCTGGAACGCCGATCTTGTGGTAATAG
ACCTCGATCTCCCTGAAATGTTTCCAGTCCAGAACATAAAGAACCACCTCGTTCACGCTT
TCAGCGGTGAAGTTTTCGCCACGATGGTTGCTGGAAAGTGGATCTACTTCGATGGAGAGT
ACCCGACCATAGATTCAGAAGAAGTGAAAAGAGAGCTGGCCCGTATAGAAAAAGAACTCT
ACTCTTCCTAAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAGAGAAGATTTT
C

Protein sequence predicted based on nucleotide sequence

MIIGNCLILKDFSSEPFWGAVEIENGTIKRVLQGEVKVDLDLSGKLVMPALFNTHTHAPM
TLLRGVAEDLSFEEWLFSKVLPIEDRLTEKMAYYGTILAQMEMARHGIAGFVDMYFHEEW
IAKAVRDFGMRALLTRGLVDSNGDDGGRLEENLKLYNEWNGFEGRIFVGFGPHSPYLCSE
EYLKRVFDTAKSLNAPVTIHLYETSKEEYDLEDILNIGLKEVKTIAAHCVHLPERYFGVL
KDIPFFVSHNPASNLKLGNGIAPVQRMIEHGMKVTLGTDGAASNNSLNLFFEMRLASLLQ
KAQNPRNLDVNTCLKMVTYDGAQAMGFKSGKIEEGWNADLVVIDLDLPEMFPVQNIKNHL
VHAFSGEVFATMVAGKWIYFDGEYPTIDSEEVKRELARIEKELYSS*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 1218
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGGGATCTGATAAAATTCATCATCATCATCATCAC
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DL41
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 282805
Recommended expression in:
Not Applicable