Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00850816
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 2889nts
Open reading frame: 1 to 2889
ATGGCAGTAGCCCCGCGACTGTTTGGGGGGCTCTGCTTCCGTTTCCGGGACCAGAATCCG
GAAGTGGCTGTTGAGGGGCGTCTTCCAATCTCGCACAGCTGCGTTGGCTGTAGAAGAGAA
CGGACGGCGATGGCGACGGTCGCAGCAAATCCAGCTGCTGCTGCGGCGGCTGTGGCGGCG
GCAGCGGCGGTGACTGAGGATAGAGAGCCACAGCACGAGGAGCTGCCAGGCCTGGACAGC
CAGTGGCGCCAGATAGAAAACGGCGAGAGTGGGCGAGAACGTCCACTGCGGGCCGGCGAA
AGCTGGTTCCTTGTGGAGAAGCACTGGTATAAGCAGTGGGAGGCATACGTGCAGGGAGGG
GACCAGGACTCCAGCACCTTCCCTGGCTGCATCAACAATGCCACACTCTTTCAAGATGAG
ATAAACTGGCGCCTCAAGGAGGGACTGGTGGAAGGCGAGGATTATGTGCTGCTCCCAGCA
GCTGCTTGGCATTACCTGGTCAGCTGGTATGGTCTAGAGCATGGCCAGCCACCCATTGAA
CGCAAGGTCATAGAGCTGCCCAACATCCAGAAGGTCGAAGTGTACCCAGTAGAACTGCTG
CTTGTCCGGCACAATGATTTGGGCAAATCTCACACTGTTCAGTTCAGCCATACCGATTCT
ATTGGCCTAGTATTGCGCACAGCTCGGGAGCGGTTTCTGGTGGAGCCCCAGGAAGACACT
CGGCTTTGGGCCAAGAACTCAGAAGGCTCTTTGGATAGGTTGTATGACACACACATCACG
GTTCTCGATGCGGCCCTTGAGACTGGGCAGTTGATCATCATGGAGACCCGCAAGAAAGAT
GGCACTTGGCCCAGCGCACAGCTGCATGTCATGAACAACAACATGTCGGAAGAGGATGAG
GACTTCAAGGGTCAGCCAGGCATCTGTGGCCTCACCAATCTGGGCAACACGTGCTTCATG
AACTCGGCCCTGCAGTGCCTCAGCAATGTGCCACAGCTCACCGAGTACTTCCTCAACAAC
TGCTACCTGGAGGAGCTCAACTTCCGCAACCCACTGGGCATGAAGGGTGAGATCGCAGAG
GCCTATGCAGACCTGGTGAAGCAGGCGTGGTCTGGCCACCACCGCTCCATTGTGCCACAT
GTGTTCAAGAACAAGGTTGGCCATTTTGCATCCCAATTTCTGGGCTACCAGCAGCATGAC
TCTCAGGAGCTGCTGTCATTCCTCCTGGACGGGCTGCATGAGGACCTTAATCGGGTGAAG
AAGAAGGAGTATGTGGAGCTGTGCGATGCTGCTGGGCGACCGGATCAGGAGGTGGCACAG
GAGGCATGGCAAAACCACAAACGGCGGAACGATTCTGTGATCGTGGACACTTTCCACGGC
CTCTTCAAGTCCACGCTGGTGTGCCCCGATTGTGGCAATGTATCTGTGACCTTCGACCCC
TTCTGCTACCTCAGTGTTCCACTGCCTATCAGCCACAAGAGGGTCTTGGAGGTCTTCTTT
ATCCCCATGGATCCGCGCCGCAAGCCAGAGCAGCACCGGCTCGTGGTCCCCAAGAAAGGC
AAGATCTCGGATCTATGTGTGGCTCTGTCCAAACACACGGGCATCTCGCCAGAGAGGATG
ATGGTGGCTGATGTCTTCAGTCACCGCTTCTATAAGCTCTATCAGCTAGAGGAGCCTCTG
AGCAGCATCTTGGACCGTGATGATATCTTCGTCTATGAGGTGTCAGGTCGCATTGAGGCC
ATTGAGGGCTCAAGAGAGGACATCGTGGTTCCTGTCTACCTGCGGGAGCGCACCCCTGCC
CGTGACTACAACAACTCCTACTACGGCCTGATGCTTTTTGGACACCCCCTCCTGGTATCA
GTGCCCCGGGACCGCTTCACCTGGGAGGGCCTGTATAACGTCCTGATGTACCGGCTCTCA
CGCTACGTGACCAAACCCAACTCAGATGATGAGGACGATGGGGATGAGAAAGAAGATGAC
GAGGAGGATAAAGATGACGTCCCTGGGCCCTCAACTGGGGGCAGCCTCCGAGACCCTGAG
CCAGAGCAGGCTGGGCCCAGCTCTGGAGTCACGAACAGGTGCCCGTTCCTCCTGGACAAT
TGCCTTGGCACATCTCAGTGGCCCCCAAGGCGACGACGCAAGCAGCTGTTCACCCTGCAG
ACGGTGAACTCCAATGGGACCAGCGACCGCACAACCTCCCCTGAAGAAGTCCATGCCCAG
CCGTACATTGCTATCGACTGGGAGCCAGAGATGAAGAAGCGTTACTATGACGAGGTAGAG
GCTGAGGGCTACGTGAAGCATGACTGCGTCGGGTACGTGATGAAGAAGGCTCCCGTGCGG
CTGCAGGAGTGCATTGAGCTCTTCACCACTGTGGAGACCCTGGAGAAGGAAAACCCCTGG
TACTGCCCTTCCTGCAAGCAGCACCAGCTGGCAACCAAGAAGCTGGACCTGTGGATGCTG
CCGGAGATTCTCATCATCCACCTGAAACGCTTTTCCTACACCAAGTTCTCCCGAGAGAAG
CTGGACACCCTCGTGGAGTTTCCTATCCGGGACCTGGACTTCTCTGAGTTTGTCATCCAG
CCACAGAATGAGTCGAATCCGGAGCTGTACAAATATGACCTCATCGCGGTTTCCAACCAT
TATGGGGGCATGCGTGATGGACACTACACAACATTTGCCTGCAACAAGGACAGCGGCCAG
TGGCACTACTTTGATGACAACAGCGTCTCCCCTGTCAATGAGAATCAGATCGAGTCCAAG
GCAGCCTATGTCCTCTTCTACCAACGCCAGGACGTGGCGCGACGCCTGCTGTCCCCGGCC
GGCTCATCTGGCGCCCCAGCCTCCCCTGCCTGCAGCTCCCCACCCAGCTCTGAGTTCATG
GATGTTAAT
Protein sequence predicted based on nucleotide sequence
MAVAPRLFGGLCFRFRDQNPEVAVEGRLPISHSCVGCRRERTAMATVAANPAAAAAAVAA AAAVTEDREPQHEELPGLDSQWRQIENGESGRERPLRAGESWFLVEKHWYKQWEAYVQGG DQDSSTFPGCINNATLFQDEINWRLKEGLVEGEDYVLLPAAAWHYLVSWYGLEHGQPPIE RKVIELPNIQKVEVYPVELLLVRHNDLGKSHTVQFSHTDSIGLVLRTARERFLVEPQEDT RLWAKNSEGSLDRLYDTHITVLDAALETGQLIIMETRKKDGTWPSAQLHVMNNNMSEEDE DFKGQPGICGLTNLGNTCFMNSALQCLSNVPQLTEYFLNNCYLEELNFRNPLGMKGEIAE AYADLVKQAWSGHHRSIVPHVFKNKVGHFASQFLGYQQHDSQELLSFLLDGLHEDLNRVK KKEYVELCDAAGRPDQEVAQEAWQNHKRRNDSVIVDTFHGLFKSTLVCPDCGNVSVTFDP FCYLSVPLPISHKRVLEVFFIPMDPRRKPEQHRLVVPKKGKISDLCVALSKHTGISPERM MVADVFSHRFYKLYQLEEPLSSILDRDDIFVYEVSGRIEAIEGSREDIVVPVYLRERTPA RDYNNSYYGLMLFGHPLLVSVPRDRFTWEGLYNVLMYRLSRYVTKPNSDDEDDGDEKEDD EEDKDDVPGPSTGGSLRDPEPEQAGPSSGVTNRCPFLLDNCLGTSQWPPRRRRKQLFTLQ TVNSNGTSDRTTSPEEVHAQPYIAIDWEPEMKKRYYDEVEAEGYVKHDCVGYVMKKAPVR LQECIELFTTVETLEKENPWYCPSCKQHQLATKKLDLWMLPEILIIHLKRFSYTKFSREK LDTLVEFPIRDLDFSEFVIQPQNESNPELYKYDLIAVSNHYGGMRDGHYTTFACNKDSGQ WHYFDDNSVSPVNENQIESKAAYVLFYQRQDVARRLLSPAGSSGAPASPACSSPPSSEFM DVN
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
