Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00850882
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 4020nts
Open reading frame: 1 to 4020
ATGTTTAGGGTTGGCTTCTTAATAATTTCTTCTTCCTCTTCTCTCTCTCCTCTTCTTTTG
GTCAGTGTTGTGCGGGTTAATACAACAAACTGTCACAAGTGTTTGTTGTCCGGGACGTAC
ATTTTCGCGGTCCTGCTAGTATGCGTGGTGTTCCACTCTGGCGCCCAGGAGAAAAACTAC
ACCATCCGAGAAGAAATTCCAGAAAACGTCCTGATAGGCAACTTGTTGAAAGACCTTAAC
TTGTCGCTGATTCCAAACAAGTCCTTGACAACTACTATGCAGTTCAAGCTAGTGTACAAG
ACCGGAGATGTGCCACTGATTCGAATTGAAGAGGATACTGGTGAGATCTTCACTACCGGC
GCTCGCATTGATCGTGAGAAATTATGTGCTGGTATCCCAAGGGATGAGCATTGCTTTTAT
GAAGTGGAGGTTGCCATTTTGCCGGATGAAATATTTAGACTGGTTAAGATACGTTTTCTG
ATAGAAGATATAAATGATAATGCACCATTGTTCCCAGCAACAGTTATCAACATATCAATT
CCAGAGAACTCGGCTATAAACTCTAAATATACTCTCCCAGCGGCTGTTGATCCTGACGTA
GGCATAAACGGAGTTCAAAACTACGAACTAATTAAGAGTCAAAACATTTTTGGCCTCGAT
GTCATTGAAACACCAGAAGGAGACAAGATGCCACAACTGATTGTTCAAAAGGAGTTAGAT
AGGGAAGAGAAGGATACCTATGTGATGAAAGTAAAGGTTGAAGATGGTGGCTTTCCTCAA
AGATCCAGTACTGCTATTTTGCAAGTAAGTGTTACTGATACAAATGACAACCACCCAGTC
TTTAAGGAGACAGAGATTGAAGTCAGTATACCAGAAAATGCTCCTGTAGGCACTTCAGTG
ACACAGCTCCATGCCACAGATGCTGACATAGGTGAAAATGCCAAGATCCACTTCTCTTTC
AGCAATCTAGTCTCCAACATTGCCAGGAGATTATTTCACCTCAATGCCACCACTGGACTT
ATCACAATCAAAGAACCACTGGATAGGGAAGAAACACCAAACCACAAGTTACTGGTTTTG
GCAAGTGATGGTGGATTGATGCCAGCAAGAGCAATGGTGCTGGTAAATGTTACAGATGTC
AATGATAATGTCCCATCCATTGACATAAGATACATCGTCAATCCTGTCAATGACACAGTT
GTTCTTTCAGAAAATATTCCACTCAACACCAAAATTGCTCTCATAACTGTGACGGATAAG
GATGCGGACCATAATGGCAGGGTGACATGCTTCACAGATCATGAAATTCCTTTCAGATTA
AGGCCAGTATTCAGTAATCAGTTCCTCCTGGAGAATGCAGCATATCTTGACTATGAGTCC
ACAAAAGAATATGCCATTAAATTACTGGCTGCAGATGCTGGCAAACCTCCTTTGAATCAG
TCAGCAATGCTCTTCATCAAAGTGAAAGATGAAAATGACAATGCTCCAGTTTTCACCCAG
TCTTTCGTAACTGTTTCTATTCCTGAGAATAACTCTCCTGGCATCCAGTTGATGAAAGTA
AGTGCAACGGATGCAGACAGTGGGCCTAATGCTGAGATCAATTACCTGCTAGGCCCTGAT
GCTCCACCTGAATTCAGCCTGGATCGTCGTACAGGCATGCTGACTGTAGTGAAGAAACTA
GATAGAGAAAAAGAGGATAAATATTTATTCACAATTCTGGCAAAAGATAATGGGGTACCA
CCCTTAACCAGCAATGTCACAGTCTTTGTAAGCATTATTGATCAGAATGACAATAGCCCA
GTTTTCACTCACAATGAATACAAATTCTATGTCCCAGAAAACCTTCCAAGGCATGGTACA
GTAGGACTAATCACTGTAACTGATCCTGATTATGGAGACAATTCTGCAGTTACGCTCTCC
ATTTTAGATGAGAATGATGACTTCACCATTGATTCACAAACTGGTGTCATCCGACCAAAT
ATTTCATTTGATAGAGAAAAACAAGAATCTTACACTTTCTATGTAAAGGCTGAGGATGGT
GGTAGAGTATCACGTTCTTCAAGTGCCAAAGTAACCATAAATGTGGTTGATGTCAATGAC
AACAAACCAGTTTTCATTGTCCCTCCTTACAACTATTCTTATGAATTGGTTCTACCGTCC
ACTAATCCAGGCACAGTGGTCTTTCAGGTAATTGCTGTTGACAATGACACTGGCATGAAT
GCAGAGGTTCGTTACAGCATTGTAGGAGGAAACACAAGAGATCTGTTTGCAATCGACCAA
GAAACAGGCAACATAACATTGATGGAGAAATGTGATGTTACAGACCTTGGTTTACACAGA
GTGTTGGTCAAAGCTAATGACTTAGGACAGCCTGATTCTCTCTTCAGTGTTGTAATTGTC
AATCTGTTCGTGAATGAGTCAGTGACCAATGCTACACTGATTAATGAACTGGTGCGCAAA
AGCATTGAAGCACCAGTGACCCCAAATACTGAGATAGCTGATGTATCCTCACCAACTAGT
GACTATGTCAAGATCCTGGTTGCAGCTGTTGCTGGCACCATAACTGTCGTTGTAGTTATT
TTCATCACTGCTGTAGTAAGATGTCGCCAGGCACCACACCTTAAGGCTGCTCAGAAAAAC
ATGCAGAATTCTGAATGGGCTACCCCAAACCCAGAAAACAGGCAGATGATAATGATGAAG
AAAAAGAAAAAGAAGAAGAAGCATTCCCCTAAGAACCTGCTGCTTAATGTTGTCACTATT
GAAGAAACTAAGGCAGATGATGTTGACAGTGATGGAAACAGAGTCACACTAGACCTTCCT
ATTGATCTAGAAGAGCAAACAATGGGAAAGTACAATTGGGTAACTACACCTACTACTTTC
AAGCCTGACAGCCCTGATTTGGCCCGACACTACAAATCTGCCTCTCCACAGCCTGCCTTC
CAAATTCAGCCTGAAACTCCCCTGAATTTGAAGCACCACATCATCCAAGAACTGCCTCTC
GATAACACCTTTGTGGCCTGTGACTCTATCTCCAATTGTTCCTCAAGCAGTTCAGATCCC
TACAGCGTTTCTGACTGTGGCTATCCAGTGACAACCTTCGAGGTACCTGTGTCCGTACAC
ACCAGACCGTCCCAGCGGCGTGTCACATTTCACCTGCCAGAAGGCTCTCAGGAAAGCAGC
AGTGATGGTGGACTGGGAGACCATGATGCAGGCAGCCTTACCAGCACATCCCATGGCCTG
CCCCTTGGCTATCCTCAGGAGGAGTACTTTGATCGTGCTACACCCAGCAATCGCACTGAA
GGGGATGGCAACTCCGATCCTGAATCTACTTTCATACCTGGACTAAAGAAAGAAATAACT
GTTCAACCAACTGTGGAAGAGGCCTCTGACAACTGCACTCAAGAATGTCTCATCTATGGC
CATTCTGATGCCTGCTGGATGCCGGCATCTCTGGATCATTCCAGCTCTTCACAAGCACAG
GCCTCTGCTCTATGCCACAGCCCACCACTGTCACAGGCCTCTACTCAGCACCACAGCCCA
CCAGTGACACAGACCATTGTTCTCTGCCACAGCCCTCCAGTGACACAGACCATCGCATTG
TGCCACAGCCCACCACCGATACAGGTGTCTGCTCTCCACCACAGTCCTCCTCTAGTGCAG
GGTACTGCACTTCACCACAGCCCACCATCAGCACAGGCCTCAGCCCTCTGCTACAGCCCT
CCTTTAGCACAGGCTGCTGCAATCAGCCACAGCTCTTCTCTGCCACAGGTTATTGCCCTC
CATCGTAGTCAGGCCCAATCATCAGTCAGTTTGCAGCAAGGTTGGGTGCAAGGTGCTAAT
GGACTATGCTCTGTTGATCAGGGAGTGCAAGGTAGTGCAACATCTCAGTTTTACACCATG
TCTGAAAGACTTCATCCCAGTGATGATTCAATTAAAGTCATTCCTTTGACAACCTTCGCT
CCACGCCAACAGGCCAGACCGTCCAGAGGTGATTCCCCCATTATGGAAACACATCCCTTG
Protein sequence predicted based on nucleotide sequence
MFRVGFLIISSSSSLSPLLLVSVVRVNTTNCHKCLLSGTYIFAVLLVCVVFHSGAQEKNY TIREEIPENVLIGNLLKDLNLSLIPNKSLTTTMQFKLVYKTGDVPLIRIEEDTGEIFTTG ARIDREKLCAGIPRDEHCFYEVEVAILPDEIFRLVKIRFLIEDINDNAPLFPATVINISI PENSAINSKYTLPAAVDPDVGINGVQNYELIKSQNIFGLDVIETPEGDKMPQLIVQKELD REEKDTYVMKVKVEDGGFPQRSSTAILQVSVTDTNDNHPVFKETEIEVSIPENAPVGTSV TQLHATDADIGENAKIHFSFSNLVSNIARRLFHLNATTGLITIKEPLDREETPNHKLLVL ASDGGLMPARAMVLVNVTDVNDNVPSIDIRYIVNPVNDTVVLSENIPLNTKIALITVTDK DADHNGRVTCFTDHEIPFRLRPVFSNQFLLENAAYLDYESTKEYAIKLLAADAGKPPLNQ SAMLFIKVKDENDNAPVFTQSFVTVSIPENNSPGIQLMKVSATDADSGPNAEINYLLGPD APPEFSLDRRTGMLTVVKKLDREKEDKYLFTILAKDNGVPPLTSNVTVFVSIIDQNDNSP VFTHNEYKFYVPENLPRHGTVGLITVTDPDYGDNSAVTLSILDENDDFTIDSQTGVIRPN ISFDREKQESYTFYVKAEDGGRVSRSSSAKVTINVVDVNDNKPVFIVPPYNYSYELVLPS TNPGTVVFQVIAVDNDTGMNAEVRYSIVGGNTRDLFAIDQETGNITLMEKCDVTDLGLHR VLVKANDLGQPDSLFSVVIVNLFVNESVTNATLINELVRKSIEAPVTPNTEIADVSSPTS DYVKILVAAVAGTITVVVVIFITAVVRCRQAPHLKAAQKNMQNSEWATPNPENRQMIMMK KKKKKKKHSPKNLLLNVVTIEETKADDVDSDGNRVTLDLPIDLEEQTMGKYNWVTTPTTF KPDSPDLARHYKSASPQPAFQIQPETPLNLKHHIIQELPLDNTFVACDSISNCSSSSSDP YSVSDCGYPVTTFEVPVSVHTRPSQRRVTFHLPEGSQESSSDGGLGDHDAGSLTSTSHGL PLGYPQEEYFDRATPSNRTEGDGNSDPESTFIPGLKKEITVQPTVEEASDNCTQECLIYG HSDACWMPASLDHSSSSQAQASALCHSPPLSQASTQHHSPPVTQTIVLCHSPPVTQTIAL CHSPPPIQVSALHHSPPLVQGTALHHSPPSAQASALCYSPPLAQAAAISHSSSLPQVIAL HRSQAQSSVSLQQGWVQGANGLCSVDQGVQGSATSQFYTMSERLHPSDDSIKVIPLTTFA PRQQARPSRGDSPIMETHPL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
