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Detailed Vector Information: p11

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: p11              
Source: Midwest Center for Structural Genomics
Description: "Bacterial (E. coli) expression vector, adds His tag, TEV cleavage site; amp resistance; restriction enzyme cloning."
Comments: None
Publications: PMID: 24012809
Title: UDP-galactopyranose mutases from Leishmania species that cause 4 visceral and cutaneous leishmaniasis
PMID: 24668168
Title: The Kinesin-12 Kif15 is a processive track-switching tetramer
Authors: PSI
Midwest Center for Structural Genomics
University of Toronto
EvNO00085126

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Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: p11

p11 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7p
Reverse:  T7t
Description: Bacterial (E. coli) expression vector, adds His tag, TEV cleavage site; ampicillin resistance; restriction enzyme cloning.
Comments: None
Size (bp): 5704
Parent Vector: None
Properties: bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type bacterial origin of replication 1164 1864
promoter T7 T7 promoter 5230 5249
repressor binding site LacO LacO sequence region 5249 5271
repressor protein gene lacI lacI cds 3764 4843
selectable marker AmpR ampicillin resistance gene 407 1066
tag His 6xHis tag 5329 5349
trxn termination sequence T7 term T7 termination sequence 5446 5492

 

Species Specific ID: None
Target GenBank: None