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Detailed Vector Information: pHM6g

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pHM6g              
Source: Berkeley Structural Genomics Center
Description: "Vector that adds N-terminal 6xHis tag, MBP, Tev protease site; kanamycin resistance; restriction enzyme cloning."
Comments: None
Publications: None
Authors: PSI
Berkeley Structural Genomics Center
Lawrence Berkeley National Laboratory
EvNO00085135

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AVAILABLE
No restriction
Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
       DyNA Vector Map

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Vector Name: pHM6g

pHM6g in advanced viewed

Synonyms: pHM, pHMG, pHMGly, pET28a.HMV.6gly
Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: Vector that adds N-terminal 6xHis tag, MBP, Tev protease site; kanamycin resistance; restriction enzyme cloning.
Comments: None
Size (bp): 0
Parent Vector: None
Properties: bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name: Berkeley Structural Genomics Center
Publications: None
Vector Map:         Vector Sequence:
Sequence: Not Available

 



Vector Features:

Type Name Description Start Position End Position
protease cleavage site TEV TEV protease cleavage site cds 0 0
tag His 6xHis tag (after cleavage GHSGGGGGGH) 0 0
tag MBP maltose binding protein cds 0 0

 

Species Specific ID: None
Target GenBank: None