Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00859264
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6264nts
Open reading frame: 1 to 6264
ATGGAGACTGAAAGTGAGAGTAGCACTTTAGGGGATGACAGTGTCTTCTGGTTGGAGTCT
GAAGTTATAATCCAGGTGACTGACTGTGAAGAGGAAGAAAGGGAAGAGAAGTTCAGGAAG
ATGAAGTCCTCAGTACATTCTGAAGAAGATGATTTTGTTCCAGAGCTACATAGAAATGTA
CACCCTCGAGAGCGGCCTGATTGGGAAGAAACTCTTAGCGCAATGGCAAGAGGCGCAGAT
GTTCCAGAGATTCCTGGAGATCTTACTCTTAAGACGTGTGGCAGTACAGCCAGTATGAAG
GTTAAGCATGTGAAAAAGTTACCCTTCACTAAAGGTCACTTTCCGAAGATGGCTGAATGT
GCGCATTTCCATTATGAGAATGTTGAGTTCGGCAGCATACAGCTTTCACTTTCAGAAGAA
CAGAATGAAGTAATGAAAAATGGCTGTGAATCTAAAGAGCTGGTCTACCTCGTGCAGATT
GCTTGTCAGGGAAAAAGTTGGATTGTTAAAAGAAGTTATGAAGATTTTCGGGTACTTGAT
AAACATCTTCATCTGTGTATTTATGACCGAAGATTTTCCCAGCTCTCAGAACTTCCCCGT
TCTGACACCCTGAAGGACAGTCCAGAGTCGGTCACTCAGATGCTTATGGCTTACCTGTCA
CGCCTTTCAGCTATCGCTGGCAACAAGATCAACTGTGGGCCCGCCCTTACCTGGATGGAG
ATTGATAATAAGGGAAATCACCTTTTGGTTCATGAGGAGTCATCCATCAACACTCCTGCT
GTCGGTGCTGCCCATGTTATCAAGAGGTACACTGCTCGGGCCCCTGACGAACTGACCTTA
GAGGTGGGAGACATTGTTTCTGTTATTGACATGCCCCCGAAAGTGTTAAGCACATGGTGG
AGAGGCAAGCACGGATTCCAGGTGGGACTCTTCCCTGGACACTGTGTTGAGTTAATTAAC
CAAAAAGTTCCCCAGTCAGTGACCAACTCAGTGCCAAAACCAGTGTCTAAAAAGCACGGC
AAGCTCATTACGTTCTTACGAACATTCATGAAGTCTCGTCCAACAAAACAGAAGCTGAAG
CAGCGGGGAATCTTGAAAGAGAGGGTGTTTGGTTGTGACCTGGGGGAACACCTTCTAAAT
TCTGGTTTTGAAGTGCCGCAGGTTCTTCAAAGCTGCACAGCATTCATTGAGAGATATGGC
ATCGTGGATGGAATCTATCGCCTTTCTGGTGTTGCCTCCAATATCCAGAGACTACGCCAT
GAATTTGACTCTGAGCACGTCCCCGACCTGACGAAAGAACCGTATGTTCAGGACATCCAT
TCTGTGGGTTCCCTATGTAAGCTGTACTTCCGGGAACTCCCAAACCCTCTGCTTACCTAC
CAGCTGTATGAGAAATTTTCTGATGCAGTTTCAGCAGCAACAGATGAAGAAAGGCTGATA
AAAATCCACGATGTCATCCAGCAGCTCCCCCCACCACACTACAGAACACTGGAGTTCCTG
ATGAGACACTTGTCTCTTCTAGCTGACTATTGTTCCATCACAAATATGCATGCAAAAAAT
CTAGCAATTGTTTGGGCTCCAAACCTGTTAAGATCAAAACAGATAGAATCTGCCTGCTTC
AGTGGAACAGCAGCTTTCATGGAAGTGAGGATTCAGTCTGTGGTTGTTGAGTTCATCCTG
AATCACGTTGATGTGCTGTTCAGCGGCAGAATCAGCATGGCCATGCAAGAGGGGGCAGCT
TCTCTATCAAGGCCCAAGTCCCTCCTGGTATCCTCTCCATCCACCAAACTGCTGACATTG
GAAGAGGCCCAGGCACGAACACAAGCTCAGGTCAATTCTCCAATTGTGACGGAAAATAAA
TATATCGAAGTAGGAGAAGGACCTGCTGCACTTCAGGGGAAATTTCATACCATAATTGAG
TTCCCACTTGAAAGAAAGAGGCCTCAAAATAAGATGAAAAAGTCTCCTGTGGGTAGCTGG
CGTTCCTTTTTCAACTTGGGGAAATCATCATCTGTTTCTAAACGAAAGCTGCAGCGGAAT
GAGAGTGAGCCTTCAGAGATGAAAGCCATGGCTCTGAAAGGTGGCAGGGCAGAAGGAACC
CTCCGTTCAGCTAAAAGTGAGGAGTCTCTTACATCTCTCCATGCAGTTGATGGTGATTCT
AAGCTCTTCCGACCCAGAAGACCCAGATCTTCCAGTGATGCACTGTCTGCCTCTTTTAAT
GGAGAAATGCTGGGGAACCGCTGTAACTCCTATGATAATCTGCCTCATGACAATGAGAGT
GAGGAGGAAGGAGGGCTGCTTCATATCCCAGCCCTTATGTCTCCTCATTCAGCTGAGGAT
GTTGACTTGAGCCCACCAGACATTGGAGTAGCCAGCCTGGATTTTGATCCAATGTCATTT
CAATGTAGTCCTCCTAAGGCCGAATCAGAATGTCTGGAGAGTGGTGCTTCCTTTTTAGAT
TCACCAGGATACTCCAAGGATAAACCAAGTGCCAATAAAAAGGATGCAGAAACAGGTAGT
AGCCAATGTCAGACTCCAGGAAGCACAGCAAGCTCTGAACCTGTCTCTCCTCTTCAGGAG
AAACTGAGTCCATTCTTTACCCTGGACTTGAGCCCAACTGAAGATAAATCATCTAAGCCA
TCCTCCTTTACTGAAAAGGTCGTCTATGCTTTCTCTCCGAAGATAGGACGGAAATTAAGC
AAATCACCTTCTATGAGCATATCTGAGCCAATTTCAGTGACCCTACCACCACGGGTGTCA
GAAGTCATTGGTACAGTCTCAAATACCACAGCTCAGAATGCATCATCTTCAACCTGGGAC
AAATGCGTTGAAGAAAGGGATGCCACAAATAGATCCCCCACCCAGATAGTAAAGATGAAA
ACAAATGAGACAGTTGCCCAAGAAGCATATGAATCTGAAGTCCAGCCCCTGGACCAGGTG
GCTGCTGAAGAAGTAGAATTGCCAGGGAAAGAGGATCAGTCTGTCTCAAGCAGTCAGAGT
AAGGCTGTAGCTTCTGGACAGACTCAGACAGGAGCAGTTACCCATGACCCCCCTCAGGAT
TCCGTTCCTGTCAGTTCAGTCTCTCTTATCCCACCACCACCGCCTCCGAAAAATGTTGCC
CGAATGTTGGCGCTAGCATTAGCTGAGTCCGCACAGCAAGCCTCAACTCAGTCATTGAAG
AGACCAGGGACCTCTCAGGCTGGGTATACAAATTATGGAGACATAGCGGTGGCTACAACT
GAAGATAATCTGTCCAGTTCTTACTCTGCAGTTGCTCTAGATAAGGCCTATTTCCAAACC
GATCGACCAGCAGAGCAGTTCCACCTCCAGAATAATGCACCAGGAAACTGTGACCATCCT
CTACCAGAGACAACAGCTACTGGGGATCCTACCCATTCCAACACAACTGAATCTGGGGAG
CAACATCACCAAGTAGACTTAACAGGGAATCAGCCACATCAAGCATATTTATCTGGGGAC
CCAGAAAAGGCCAGAATTACTTCAGTTCCCTTAGACTCAGAGAAGTCTGATGATCATGTA
AGTTTCCCTGAAGACCAGTCTGGGAAGAACAGTATGCCAACTGTCTCCTTCTTGGATCAG
GACCAGTCTCCACCCCGTTTCTACAGTGGAGATCAGCCTCCTTCTTATCTTGGTGCAAGT
GTGGATAAACTCCATCACCCTTTAGAATTTGCAGACAAATCTCCCACACCTCCTAATTTA
CCTAGCGATAAAATCTACCCTCCTTCTGGGTCCCCCGAAGAGAATACCAGCACAGCCACC
ATGACTTACATGACAACTACTCCAGCAACAGCCCAAATGAGCACCAAGGAAGCCAGCTGG
GATGTGGCTGAACAACCCACCACTGCTGATTTTGCTGCTGCCACACTTCAGCGCACGCAC
AGAACTAATCGTCCCCTTCCCCCTCCGCCTTCCCAGAGATCTGCAGAGCAGCCACCAGTT
GTGGGGCAGGTACAAGCAGCAACCAATATAGGATTAAATAATTCCCACAAGGTTCAAGGA
GTAGTTCCAGTTCCAGAGAGGCCACCTGAACCTCGAGCCATGGATGACCCTGCGTCTGCC
TTCATCAGTGACAGTGGTGCTGCTGCTGCTCAGTGTCCCATGGCTACAGCTGTCCAGCCA
GGCCTGCCTGAGAAAGTGCGGGACGGTGCCCGGGTCCCGCTGCTGCACCTGCGCGCCGAG
TCTGTCCCTGCGCATCCCTGTGGCTTTCCTGCACCACTGCCCCCCACCAGGATGATGGAG
AGTAAGATGATTGCTGCCATACACTCCAGCAGTGCAGATGCCACCAGCAGTTCAAATTAT
CATTCCTTTGTCACTGCTTCATCCACCTCTGTGGACGATGCATTGCCTTTACCACTTCCT
GTCCCACAACCTAAGCATGCTTCTCAGAAAACAGTTTACTCCTCCTTTGCTAGGCCCGAT
GTCACCACTGAACCCTTTGGTCCAGATAACTGTTTGCATTTCAATATGACTCCAAACTGC
CAGTACCGTCCCCAGAGTGTACCTCCCCATCACAATAAATTGGAGCAGCACCAAGTGTAT
GGTGCCAGGTCAGAGCCACCAGCCTCCATGGGTCTTCGTTATAACACATATGTGGCCCCA
GGAAGAAACGCATCTGGACACCACTCCAAGCCATGCAGCCGGGTCGAGTATGTGTCTTCT
TTGAGCTCCTCTGTCAGGAATACCTGTTACCCCGAAGACATTCCACCGTACCCTACCATC
CGGAGAGTGCAGTCTCTCCATGCTCCGCCGTCTTCCATGATTCGCTCTGTTCCCATTTCA
CGGACAGAAGTTCCCCCAGATGATGAGCCAGCCTACTGCCCAAGACCTCTGTACCAATAT
AAGCCATATCAGTCCTCCCAGGCCCGCTCAGATTATCATGTCACTCAGCTTCAGCCTTAC
TTTGAGAATGGCCGGGTCCACTACAGGTATAGCCCATATTCCAGTTCTTCTAGTTCCTAT
TACAGTCCAGATGGGGCCCTGTGTGATGTGGATGCCTATGGCACAGTCCAGTTGAGACCC
CTTCACCGCCTTCCCAATCGAGACTTTGCTTTCTACAATCCTAGGCTGCAAGGAAAGAGC
TTGTACAGTTATGCTGGTTTGGCTCCACGTCCCCGGGCCAACGTGACTGGCTATTTCTCT
CCCAACGACCATAATGTAGTCAGCATGCCTCCGGCTGCTGATGTGAAGCACACCTACACC
TCATGGGATCTTGAGGACATGGAAAAATACCGCATGCAGTCCATCCGGAGAGAGAGCCGT
GCTCGGCAGAAGGTGAAAGGGCCTGTCATGTCCCAATATGATAACATGACCCCGGCGGTG
CAGGACGACTTGGGTGGGATCTATGTCATCCATCTGCGTAGTAAATCAGATCCTGGGAAA
ACTGGACTTCTCTCAGTGGCAGAAGGAAAGGAGAGCCGCCATGCAGCCAAGGCCATCAGT
CCCGAGGGAGAGGACCGCTTCTATAGGAGGCATCCCGAGGCAGAGATGGACAGAGCCCAC
CATCACGGAGGCCATGGTAGCACGCAGCCGGAGAAGCCATCCCTGCCTCAGAAGCAGAGC
AGCCTGAGGAGCAGGAAGCTTCCTGACATGGGCTGCAGTCTTCCTGAGCACAGGGCACAC
CAAGAAGCAAGCCATAGGCAGTTCTGTGAGTCAAAGAATGGGCCCCCTTATCCCCAGGGA
GCTGGCCAGTTAGATTATGGGTCCAAAGGGATTCCAGACACTTCTGAGCCAGTCAGCTAC
CACAACTCTGGAGTAAAATATGCTGCATCCGGGCAAGAATCTTTAAGACTGAACCACAAA
GAGGTAAGGCTCTCCAAAGAGATGGAGCGACCCTGGGTTAGGCAGCCTTCTGCCCCAGAG
AAACACTCCAGAGACTGCTACAAGGAGGAAGAACACCTCACTCAGTCAATCGTCCCACCC
CCTAAACCAGAGAGGAGTCATAGCCTCAAACTCCATCATACCCAGAACGTGGAGAGGGAC
CCCAGTGTGCTGTACCAGTACCAACCACACGGCAAGCGCCAGAGCAGTGTGACTGTTGTG
TCCCAGTATGATAACCTGGAAGATTACCACTCCCTGCCTCAGCACCAGCGAGGAGTCTTT
GGAGGGGGCGGCATGGGGACGTATGTGCCCCCTGGCTTTCCCCATCCACAGAGCAGGACC
TATGCTACAGCGTTGGGTCAAGGGGCCTTCCTGCCCGCAGAGTTGTCCTTGCAGCATCCT
GAAACACAGATCCATGCAGAATTG
Protein sequence predicted based on nucleotide sequence
METESESSTLGDDSVFWLESEVIIQVTDCEEEEREEKFRKMKSSVHSEEDDFVPELHRNV HPRERPDWEETLSAMARGADVPEIPGDLTLKTCGSTASMKVKHVKKLPFTKGHFPKMAEC AHFHYENVEFGSIQLSLSEEQNEVMKNGCESKELVYLVQIACQGKSWIVKRSYEDFRVLD KHLHLCIYDRRFSQLSELPRSDTLKDSPESVTQMLMAYLSRLSAIAGNKINCGPALTWME IDNKGNHLLVHEESSINTPAVGAAHVIKRYTARAPDELTLEVGDIVSVIDMPPKVLSTWW RGKHGFQVGLFPGHCVELINQKVPQSVTNSVPKPVSKKHGKLITFLRTFMKSRPTKQKLK QRGILKERVFGCDLGEHLLNSGFEVPQVLQSCTAFIERYGIVDGIYRLSGVASNIQRLRH EFDSEHVPDLTKEPYVQDIHSVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLI KIHDVIQQLPPPHYRTLEFLMRHLSLLADYCSITNMHAKNLAIVWAPNLLRSKQIESACF SGTAAFMEVRIQSVVVEFILNHVDVLFSGRISMAMQEGAASLSRPKSLLVSSPSTKLLTL EEAQARTQAQVNSPIVTENKYIEVGEGPAALQGKFHTIIEFPLERKRPQNKMKKSPVGSW RSFFNLGKSSSVSKRKLQRNESEPSEMKAMALKGGRAEGTLRSAKSEESLTSLHAVDGDS KLFRPRRPRSSSDALSASFNGEMLGNRCNSYDNLPHDNESEEEGGLLHIPALMSPHSAED VDLSPPDIGVASLDFDPMSFQCSPPKAESECLESGASFLDSPGYSKDKPSANKKDAETGS SQCQTPGSTASSEPVSPLQEKLSPFFTLDLSPTEDKSSKPSSFTEKVVYAFSPKIGRKLS KSPSMSISEPISVTLPPRVSEVIGTVSNTTAQNASSSTWDKCVEERDATNRSPTQIVKMK TNETVAQEAYESEVQPLDQVAAEEVELPGKEDQSVSSSQSKAVASGQTQTGAVTHDPPQD SVPVSSVSLIPPPPPPKNVARMLALALAESAQQASTQSLKRPGTSQAGYTNYGDIAVATT EDNLSSSYSAVALDKAYFQTDRPAEQFHLQNNAPGNCDHPLPETTATGDPTHSNTTESGE QHHQVDLTGNQPHQAYLSGDPEKARITSVPLDSEKSDDHVSFPEDQSGKNSMPTVSFLDQ DQSPPRFYSGDQPPSYLGASVDKLHHPLEFADKSPTPPNLPSDKIYPPSGSPEENTSTAT MTYMTTTPATAQMSTKEASWDVAEQPTTADFAAATLQRTHRTNRPLPPPPSQRSAEQPPV VGQVQAATNIGLNNSHKVQGVVPVPERPPEPRAMDDPASAFISDSGAAAAQCPMATAVQP GLPEKVRDGARVPLLHLRAESVPAHPCGFPAPLPPTRMMESKMIAAIHSSSADATSSSNY HSFVTASSTSVDDALPLPLPVPQPKHASQKTVYSSFARPDVTTEPFGPDNCLHFNMTPNC QYRPQSVPPHHNKLEQHQVYGARSEPPASMGLRYNTYVAPGRNASGHHSKPCSRVEYVSS LSSSVRNTCYPEDIPPYPTIRRVQSLHAPPSSMIRSVPISRTEVPPDDEPAYCPRPLYQY KPYQSSQARSDYHVTQLQPYFENGRVHYRYSPYSSSSSSYYSPDGALCDVDAYGTVQLRP LHRLPNRDFAFYNPRLQGKSLYSYAGLAPRPRANVTGYFSPNDHNVVSMPPAADVKHTYT SWDLEDMEKYRMQSIRRESRARQKVKGPVMSQYDNMTPAVQDDLGGIYVIHLRSKSDPGK TGLLSVAEGKESRHAAKAISPEGEDRFYRRHPEAEMDRAHHHGGHGSTQPEKPSLPQKQS SLRSRKLPDMGCSLPEHRAHQEASHRQFCESKNGPPYPQGAGQLDYGSKGIPDTSEPVSY HNSGVKYAASGQESLRLNHKEVRLSKEMERPWVRQPSAPEKHSRDCYKEEEHLTQSIVPP PKPERSHSLKLHHTQNVERDPSVLYQYQPHGKRQSSVTVVSQYDNLEDYHSLPQHQRGVF GGGGMGTYVPPGFPHPQSRTYATALGQGAFLPAELSLQHPETQIHAEL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
