Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00859333
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4512nts
Open reading frame: 1 to 4512
ATGGCCGCGCCTGCTGAGCCCTGCGCGGGGCAGGGGGTCTGGAACCAGACAGAGCCTGAA
CCTGCCGCCACCAGCCTGCTGAGCCTGTGCTTCCTGAGAACAGCAGGGGTCTGGGTACCC
CCCATGTACCTCTGGGTCCTTGGTCCCATCTACCTCCTCTTCATCCACCACCATGGCCGG
GGCTACCTCCGGATGTCCCCACTCTTCAAAGCCAAGATGGTGCTTGGATTCGCCCTCATA
GTCCTGTGTACCTCCAGCGTGGCTGTCGCTCTTTGGAAAATCCAACAGGGAACGCCTGAG
GCCCCAGAATTCCTCATTCATCCTACTGTGTGGCTCACCACGATGAGCTTCGCAGTGTTC
CTGATTCACACCGAGAGGAAAAAGGGAGTCCAGTCATCTGGAGTGCTGTTTGGTTACTGG
CTTCTCTGCTTTGTCTTGCCAGCTACCAACGCTGCCCAGCAGGCCTCCGGAGCGGGCTTC
CAGAGCGACCCTGTCCGCCACCTGTCCACCTACCTATGCCTGTCTCTGGTGGTGGCACAG
TTTGTGCTGTCCTGCCTGGCGGATCAACCCCCCTTCTTCCCTGAAGACCCCCAGCAGTCT
AACCCCTGTCCAGAGACTGGGGCAGCCTTCCCCTCCAAAGCCACGTTCTGGTGGGTTTCT
GGCCTGGTCTGGAGGGGATACAGGAGGCCACTGAGACCAAAAGACCTCTGGTCGCTTGGG
AGAGAAAACTCCTCAGAAGAACTTGTTTCCCGGCTTGAAAAGGAGTGGATGAGGAACCGC
AGTGCAGCCCGGAGGCACAACAAGGCAATAGCATTTAAAAGGAAAGGCGGCAGTGGCATG
AAGGCTCCAGAGACCGAGCCCTTCCTACGGCAAGAAGGGAGCCAGTGGCGCCCACTGCTG
AAGGCCATCTGGCAGGTGTTCCATTCTACCTTCCTCCTGGGGACCCTCAGCCTCATCATC
AGTGATGTCTTCAGGTTCACTGTCCCCAAGCTGCTCAGCCTTTTCCTGGAGTTTATTGGT
GATCCCAAGCCTCCAGCCTGGAAGGGCTACCTCCTCGCCGTGCTGATGTTCCTCTCAGCC
TGCCTGCAAACGCTGTTTGAGCAGCAGAACATGTACAGGCTCAAGGTGCTGCAGATGAGG
TTGCGGTCGGCCATCACTGGCCTGGTGTACAGAAAGGTCCTGGCTCTGTCCAGCGGCTCC
AGAAAGGCCAGTGCGGTGGGTGATGTGGTCAATCTGGTGTCCGTGGACGTGCAGCGGCTG
ACCGAGAGCGTCCTCTACCTCAACGGGCTGTGGCTGCCTCTCGTCTGGATCGTGGTCTGC
TTCGTCTATCTCTGGCAGCTCCTGGGGCCCTCCGCCCTCACTGCCATCGCTGTCTTCCTG
AGCCTCCTCCCTCTGAATTTCTTCATCTCCAAGAAAAGGAACCACCATCAGGAGGAGCAA
ATGAGGCAGAAGGACTCACGGGCACGGCTCACCAGCTCTATCCTCAGGAACTCGAAGACC
ATCAAGTTCCATGGCTGGGAGGGAGCCTTTCTGGACAGAGTCCTGGGCATCCGAGGCCAG
GAGCTGGGCGCCTTGCGGACCTCCGGCCTCCTCTTCTCTGTGTCGCTGGTGTCCTTCCAA
GTGTCTACATTTCTGGTCGCACTGGTGGTGTTTGCTGTCCACACTCTGGTGGCCGAGAAT
GCTATGAATGCAGAGAAAGCCTTTGTGACTCTCACAGTTCTCAACATCCTCAACAAGGCC
CAGGCTTTCCTGCCCTTCTCCATCCACTCCCTCGTCCAGGCCCGGGTGTCCTTTGACCGT
CTGGTCACCTTCCTCTGCCTGGAAGAAGTTGACCCTGGTGTCGTAGACTCAAGTTCCTCT
GGAAGCGCTGCCGGGAAGGATTGCATCACCATACACAGTGCCACCTTCGCCTGGTCCCAG
GAAAGCCCTCCCTGCCTCCACAGAATAAACCTCACGGTGCCCCAGGGCTGTCTGCTGGCT
GTTGTCGGTCCAGTGGGGGCAGGGAAGTCCTCCCTGCTGTCCGCCCTCCTTGGGGAGCTG
TCAAAGGTGGAGGGGTTCGTGAGCATCGAGGGTGCTGTGGCCTACGTGCCCCAGGAGGCC
TGGGTGCAGAACACCTCTGTGGTAGAGAATGTGTGCTTCGGGCAGGAGCTGGACCCACCC
TGGCTGGAGAGAGTACTAGAAGCCTGTGCCCTGCAGCCAGATGTGGACAGCTTCCCTGAG
GGAATCCACACTTCAATTGGGGAGCAGGGCATGAATCTCTCCGGAGGCCAGAAGCAGCGG
CTGAGCCTGGCCCGGGCTGTATACAGAAAGGCAGCTGTGTACCTGCTGGATGACCCCCTG
GCGGCCCTGGATGCCCACGTTGGCCAGCATGTCTTCAACCAGGTCATTGGGCCTGGTGGG
CTACTCCAGGGAACAACACGGATTCTCGTGACGCACGCACTCCACATCCTGCCCCAGGCT
GATTGGATCATAGTGCTGGCAAATGGGGCCATCGCAGAGATGGGTTCCTACCAGGAGCTT
CTGCAGAGGAAGGGGGCCCTCGTGTGTCTTCTGGATCAAGCCAGACAGCCAGGAGATAGA
GGAGAAGGAGAAACAGAACCTGGGACCAGCACCAAGGACCCCAGAGGCACCTCTGCAGGC
AGGAGGCCCGAGCTTAGACGCGAGAGGTCCATCAAGTCAGTCCCTGAGAAGGACCGTACC
ACTTCAGAAGCCCAGACAGAGGTTCCTCTGGATGACCCTGACAGGGCAGGATGGCCAGCA
GGAAAGGACAGCATCCAATACGGCAGGGTGAAGGCCACAGTGCACCTGGCCTACCTGCGT
GCCGTGGGCACCCCCCTCTGCCTCTACGCACTCTTCCTCTTCCTCTGCCAGCAAGTGGCC
TCCTTCTGCCGGGGCTACTGGCTGAGCCTGTGGGCGGACGACCCTGCAGTAGGTGGGCAG
CAGACGCAGGCAGCCCTGCGTGGCGGGATCTTCGGGCTCCTCGGCTGTCTCCAAGCCATT
GGGCTGTTTGCCTCCATGGCTGCGGTGCTCCTAGGTGGGGCCCGGGCATCCAGGTTGCTC
TTCCAGAGGCTCCTGTGGGATGTGGTGCGATCTCCCATCAGCTTCTTTGAGCGGACACCC
ATTGGTCACCTGCTAAACCGCTTCTCCAAGGAGACAGACACGGTTGACGTGGACATTCCA
GACAAACTCCGGTCCCTGCTGATGTACGCCTTTGGACTCCTGGAGGTCAGCCTGGTGGTG
GCAGTGGCTACCCCACTGGCCACTGTGGCCATCCTGCCACTGTTTCTCCTCTACGCTGGG
TTTCAGAGCCTGTATGTGGTTAGCTCATGCCAGCTGAGACGCTTGGAGTCAGCCAGCTAC
TCGTCTGTCTGCTCCCACATGGCTGAGACGTTCCAGGGCAGCACAGTGGTCCGGGCATTC
CGAACCCAGGCCCCCTTTGTGGCTCAGAACAATGCTCGCGTAGATGAAAGCCAGAGGATC
AGTTTCCCGCGACTGGTGGCTGACAGGTGGCTTGCGGCCAATGTGGAGCTCCTGGGGAAT
GGCCTGGTGTTTGCAGCTGCCACGTGTGCTGTGCTGAGCAAAGCCCACCTCAGTGCTGGC
CTCGTGGGCTTCTCTGTCTCTGCTGCCCTCCAGGTGACCCAGACACTGCAGTGGGTTGTT
CGCAACTGGACAGACCTAGAGAACAGCATCGTGTCAGTGGAGCGGATGCAGGACTATGCC
TGGACGCCCAAGGAGGCTCCCTGGAGGCTGCCCACATGTGCAGCTCAGCCCCCCTGGCCT
CAGGGCGGGCAGATCGAGTTCCGGGACTTTGGGCTAAGATACCGACCTGAGCTCCCGCTG
GCTGTGCAGGGCGTGTCCTTCAAGATCCACGCAGGAGAGAAGGTGGGCATCGTTGGCAGG
ACCGGGGCAGGGAAGTCCTCCCTGGCCAGTGGGCTGCTGCGGCTCCAGGAGGCAGCTGAG
GGTGGGATCTGGATCGACGGGGTCCCCATTGCCCACGTGGGGCTGCACACACTGCGCTCC
AGGATCAGCATCATCCCCCAGGACCCCATCCTGTTCCCTGGCTCTCTGCGGATGAACCTC
GACCTGCTGCAGGAGCACTCGGACGAGGCTATCTGGGCAGCCCTGGAGACGGTGCAGCTC
AAAGCCTTGGTGGCCAGCCTGCCCGGCCAGCTGCAGTACAAGTGTGCTGACCGAGGCGAG
GACCTGAGCGTGGGCCAGAAACAGCTCCTGTGTCTGGCACGTGCCCTTCTCCGGAAGACC
CAGATCCTCATCCTGGACGAGGCTACTGCTGCCGTGGACCCTGGCACGGAGCTGCAGATG
CAGGCCATGCTCGGGAGCTGGTTTGCACAGTGCACTGTGCTGCTCATTGCCCACCGCCTG
CGCTCCGTGATGGACTGTGCCCGGGTTCTGGTCATGGACAAGGGGCAGGTGGCAGAGAGC
GGCAGCCCGGCCCAGCTGCTGGCCCAGAAGGGCCTGTTTTACAGACTGGCCCAGGAGTCA
GGCCTGGTCTTG
Protein sequence predicted based on nucleotide sequence
MAAPAEPCAGQGVWNQTEPEPAATSLLSLCFLRTAGVWVPPMYLWVLGPIYLLFIHHHGR GYLRMSPLFKAKMVLGFALIVLCTSSVAVALWKIQQGTPEAPEFLIHPTVWLTTMSFAVF LIHTERKKGVQSSGVLFGYWLLCFVLPATNAAQQASGAGFQSDPVRHLSTYLCLSLVVAQ FVLSCLADQPPFFPEDPQQSNPCPETGAAFPSKATFWWVSGLVWRGYRRPLRPKDLWSLG RENSSEELVSRLEKEWMRNRSAARRHNKAIAFKRKGGSGMKAPETEPFLRQEGSQWRPLL KAIWQVFHSTFLLGTLSLIISDVFRFTVPKLLSLFLEFIGDPKPPAWKGYLLAVLMFLSA CLQTLFEQQNMYRLKVLQMRLRSAITGLVYRKVLALSSGSRKASAVGDVVNLVSVDVQRL TESVLYLNGLWLPLVWIVVCFVYLWQLLGPSALTAIAVFLSLLPLNFFISKKRNHHQEEQ MRQKDSRARLTSSILRNSKTIKFHGWEGAFLDRVLGIRGQELGALRTSGLLFSVSLVSFQ VSTFLVALVVFAVHTLVAENAMNAEKAFVTLTVLNILNKAQAFLPFSIHSLVQARVSFDR LVTFLCLEEVDPGVVDSSSSGSAAGKDCITIHSATFAWSQESPPCLHRINLTVPQGCLLA VVGPVGAGKSSLLSALLGELSKVEGFVSIEGAVAYVPQEAWVQNTSVVENVCFGQELDPP WLERVLEACALQPDVDSFPEGIHTSIGEQGMNLSGGQKQRLSLARAVYRKAAVYLLDDPL AALDAHVGQHVFNQVIGPGGLLQGTTRILVTHALHILPQADWIIVLANGAIAEMGSYQEL LQRKGALVCLLDQARQPGDRGEGETEPGTSTKDPRGTSAGRRPELRRERSIKSVPEKDRT TSEAQTEVPLDDPDRAGWPAGKDSIQYGRVKATVHLAYLRAVGTPLCLYALFLFLCQQVA SFCRGYWLSLWADDPAVGGQQTQAALRGGIFGLLGCLQAIGLFASMAAVLLGGARASRLL FQRLLWDVVRSPISFFERTPIGHLLNRFSKETDTVDVDIPDKLRSLLMYAFGLLEVSLVV AVATPLATVAILPLFLLYAGFQSLYVVSSCQLRRLESASYSSVCSHMAETFQGSTVVRAF RTQAPFVAQNNARVDESQRISFPRLVADRWLAANVELLGNGLVFAAATCAVLSKAHLSAG LVGFSVSAALQVTQTLQWVVRNWTDLENSIVSVERMQDYAWTPKEAPWRLPTCAAQPPWP QGGQIEFRDFGLRYRPELPLAVQGVSFKIHAGEKVGIVGRTGAGKSSLASGLLRLQEAAE GGIWIDGVPIAHVGLHTLRSRISIIPQDPILFPGSLRMNLDLLQEHSDEAIWAALETVQL KALVASLPGQLQYKCADRGEDLSVGQKQLLCLARALLRKTQILILDEATAAVDPGTELQM QAMLGSWFAQCTVLLIAHRLRSVMDCARVLVMDKGQVAESGSPAQLLAQKGLFYRLAQES GLVL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
