Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00859539
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 7401nts
Open reading frame: 1 to 7401
ATGGGGAGAAAAACGAGCCGCGTGCAGCAGGGGCCGCCTCGGTCCCCTGCCTGCTCCGCT
CACGGCGAGCGTTCCTGGGGCCTCGAGTTTCCCCCCTTCCGCTGGCTGCTCGGAATCGCG
GGACGGACCCACCTGGCCGCCCTGCCTCCCTTCCAGTGGGTCCTGGCCTTCGAGATCTTC
ATCCCCCTGGTGCTGTTCTTTATCCTGCTGGGGCTGCGACAGAAGAAGCCCACCATCTCC
GTGAAGGAAGTCTCCTTCTACACAGCGGCGCCCCTGACGTCTGCCGGCATCCTGCCTGTC
ATGCAATCGCTGTGCCCGGACGGCCAGCGAGACGAGTTCGGCTTCCTGCAGTACGCCAAC
TCCACGGTCACGCAGCTGCTTGAGCGCCTGGACCGCGTGGTGGAGGAAGGCAACCTGTTT
GACCCAGCGCGGCCCAGCCTGGGCTCAGAGCTCGAGGCCCTACGCCAGCATCTGGAGGCC
CTCAGTGCGGGCCCGGGCACCTCGGGGAGCCACCTGGACAGATCCACAGTGTCTTCCTTC
TCTCTGGACTCGGTGGCCAGAAACCCGCAGGAGCTCTGGCGTTTCCTGACGCAAAACTTG
TCGCTGCCCAATAGCACGGCCCAAGCACTCTTGGCCGCCCGTGTGGACCCGCCCGAGGTC
TACCACCTGCTCTTTGGTCCCTCATCTGCCCTGGATTCACAGTCTGGCCTCCACAAGGGT
CAGGAGCCCTGGAGCCGCCTAGGGGGCAATCCCCTGTTCCGGATGGAGGAGCTGCTGCTG
GCTCCTGCCCTCCTGGAGCAGCTCACCTGCACGCCGGGCTCGGGGGAGCTGGGCCGGATC
CTCACTGTGCCTGAGAGTCAGAAGGGAGCCCTGCAGGGCTACCGGGATGCTGTCTGCAGT
GGGCAGGCTGCTGCGCGTGCCAGGCGCTTCTCTGGGCTGTCTGCTGAGCTCCGGAACCAG
CTGGACGTGGCCAAGGTCTCCCAGCAGCTGGGCCTGGATGCCCCCAACGGCTCGGACTCC
TCGCCACAGGCGCCACCCCCACGGAGGCTGCAGGCGCTTCTGGGGGACCTGCTGGATGCC
CAGAAGGTTCTGCAGGATGTGGATGTCCTGTCGGCCCTGGCCCTGCTACTGCCCCAGGGT
GCCTGCACTGGCCGGACCCCCGGACCCCCAGCCAGTGGTGCGGGTGGGGCGGCCAATGGC
ACTGGGGCAGGGGCAGTCATGGGCCCCAACGCCACCGCTGAGGAGGGCGCACCCTCTGCT
GCAGCACTGGCCACCCCGGACACGCTGCAGGGCCAGTGCTCAGCCTTCGTACAGCTCTGG
GCCGGCCTGCAGCCCATCTTGTGTGGCAACAACCGCACCATTGAACCCGAGGCGCTGCGG
CGGGGCAACATGAGCTCCCTGGGCTTCACGAGCAAGGAGCAGCGGAACCTGGGCCTCCTC
GTGCACCTCATGACCAGCAACCCCAAAATCCTGTACGCGCCTGCGGGCTCTGAGGTCGAC
CGCGTCATCCTCAAGGCCAACGAGACTTTTGCTTTTGTGGGCAACGTGACTCACTATGCC
CAGGTCTGGCTCAACATCTCGGCGGAGATCCGCAGCTTCCTGGAGCAGGGCAGGCTGCAG
CAACACCTGCGCTGGCTGCAGCAGTATGTAGCAGAGCTGCGGCTGCACCCCGAGGCACTG
AACCTGTCACTGGATGAGCTGCCGCCGGCCCTGAGACAGGACAACTTCTCGCTGCCCAGT
GGCATGGCCCTCCTGCAGCAGCTGGATACCATTGACAACGCGGCCTGCGGCTGGATCCAG
TTCATGTCCAAGGTGAGCGTGGACATCTTCAAGGGCTTCCCCGACGAGGAGAGCATTGTC
AACTACACCCTCAACCAGGCCTACCAGGACAACGTCACTGTTTTTGCCAGTGTGATCTTC
CAGACCCGGAAGGACGGCTCGCTCCCGCCTCACGTGCACTACAAGATCCGCCAGAACTCC
AGCTTCACCGAGAAAACCAACGAGATCCGCCGCGCCTACTGGCGGCCTGGGCCCAATACT
GGCGGCCGCTTCTACTTCCTCTACGGCTTCGTCTGGATCCAGGACATGATGGAGCGCGCC
ATCATCGACACTTTTGTGGGGCACGATGTGGTGGAGCCAGGCAGCTACGTGCAGATGTTC
CCCTACCCCTGCTACACACGCGATGACTTCCTGTTTGTCATTGAGCACATGATGCCGCTG
TGCATGGTGATCTCCTGGGTCTACTCCGTGGCCATGACCATCCAGCACATCGTGGCGGAG
AAGGAGCACCGGCTCAAGGAGGTGATGAAGACCATGGGCCTGAACAACGCGGTGCACTGG
GTGGCCTGGTTCATCACCGGCTTTGTGCAGCTGTCCATCTCCGTGACAGCACTCACCGCC
ATCCTGAAGTACGGCCAGGTGCTTATGCACAGCCACGTGGTCATCATCTGGCTCTTCCTG
GCAGTCTACGCGGTGGCCACCATCATGTTCTGCTTCCTGGTGTCTGTGCTGTACTCCAAG
GCCAAGCTGGCCTCGGCCTGCGGTGGCATCATCTACTTCCTGAGCTACGTGCCCTACATG
TACGTGGCGATCCGAGAGGAGGTGGCGCATGATAAGATCACGGCCTTCGAGAAGTGCATC
GCGTCCCTCATGTCCACGACGGCCTTTGGTCTGGGCTCTAAGTACTTCGCGCTGTATGAG
GTGGCCGGCGTGGGCATCCAGTGGCACACCTTCAGCCAGTCCCCGGTGGAGGGGGACGAC
TTCAACTTGCTCCTGGCTGTCACCATGCTGATGGTGGACGCCGTGGTCTATGGCATCCTC
ACGTGGTACATTGAGGCTGTGCACCCAGGCATGTACGGGCTGCCCCGGCCCTGGTACTTC
CCACTGCAGAAGTCCTACTGGCTGGGCAGTGGGCGGACAGAAGCCTGGGAGTGGAGCTGG
CCGTGGGCACGCACCCCCCGCCTCAGTGTCATGGAGGAGGACCAGGCCTGTGCCATGGAG
AGCCGGCGCTTTGAGGAGACCCGTGGCATGGAGGAGGAGCCCACCCACCTGCCTCTGGTT
GTCTGCGTGGACAAACTCACCAAGGTCTACAAGGACGACAAGAAGCTGGCCCTGAACAAG
CTGAGCCTGAACCTCTACGAGAACCAGGTGGTCTCCTTCTTGGGCCACAACGGGGCGGGC
AAGACCACCACCATGTCCATCCTGACCGGCCTGTTCCCTCCAACGTCGGGTTCCGCCACC
ATCTACGGGCACGACATCCGCACGGAGATGGATGAGATCCGCAAGAACCTGGGCATGTGC
CCGCAGCACAATGTGCTCTTTGACCGGCTCACGGTGGAGGAACACCTCTGGTTCTACTCA
CGGCTCAAGAGCATGGCTCAGGAGGAGATCCGCAGAGAGATGGACAAGATGATCGAGGAC
CTGGAGCTCTCCAACAAACGGCACTCACTGGTGCAGACATTGTCGGGTGGCATGAAGCGC
AAGCTGTCCGTGGCCATCGCCTTCGTGGGCGGCTCTCGCGCCATCATCCTGGACGAGCCC
ACGGCGGGCGTGGACCCCTACGCGCGCCGCGCCATCTGGGACCTCATCCTGAAGTACAAG
CCAGGCCGCACCATCCTTCTGTCCACCCACCACATGGATGAGGCTGACCTGCTTGGGGAC
CGCATTGCCATCATCTCCCATGGGAAGCTCAAGTGCTGCGGCTCCCCGCTCTTCCTCAAG
GGCACCTATGGCGACGGGTACCGCCTCACGCTGGTCAAGCGGCCCGCCGAGCCGGGGGGC
CCCCAAGAGCCAGGGCTGGCATCCAGCCCCCCAGGTCGGGCCCCGCTGAGCAGCTGCTCC
GAGCTCCAGGTGTCCCAGTTCATCCGCAAGCATGTGGCCTCCTGCCTGCTGGTCTCAGAC
ACAAGCACGGAGCTCTCCTACATCCTGCCCAGCGAGGCCGCCAAGAAGGGGGCTTTCGAG
CGCCTCTTCCAGCACCTGGAGCGCAGCCTGGATGCACTGCACCTCAGCAGCTTCGGGCTG
ATGGACACGACCCTGGAGGAAGTGTTCCTCAAGGTGTCGGAGGAGGATCAGTCGCTGGAG
AACAGTGAGGCCGATGTGAAGGAGTCCAGGAAGGATGTGCTCCCTGGGGCGGAGGGCCCG
GCGTCTGGGGAGGGTCACGCTGGCAATCTGGCCCGGTGCTCGGAGCTGACCCAGTCGCAG
GCATCGCTGCAGTCGGCGTCATCTGTGGGCTCTGCCCGTGGCGACGAGGGAGCTGGCTAC
ACCGACGTCTATGGCGACTACCGCCCCCTCTTTGATAACCCACAGGACCCAGACAATGTC
AGCCTGCAAGAGGTGGAGGCAGAGGCCCTGTCGAGGGTCGGCCAGGGCAGCCGCAAGCTG
GACGGCGGGTGGCTGAAGGTGCGCCAGTTCCACGGGCTGCTGGTCAAACGCTTCCACTGC
GCCCGCCGCAACTCCAAGGCACTCTTCTCCCAGATCTTGCTGCCAGCCTTCTTCGTCTGC
GTGGCCATGACCGTGGCCCTGTCCGTCCCGGAGATTGGTGATCTGCCCCCGCTGGTCCTG
TCACCTTCCCAGTACCACAACTACACCCAGCCCCGTGGCAATTTCATCCCCTACGCCAAC
GAGGAGCGCCGCGAGTACCGGCTGCGGCTATCGCCCGACGCCAGCCCCCAGCAGCTCGTG
AGCACGTTCCGGCTGCCGTCGGGGGTGGGTGCCACCTGCGTGCTCAAGTCTCCCGCCAAC
GGCTCGCTGGGGCCCACGTTGAACCTGAGCAGCGGGGAGTCGCGCCTGCTGGCGGCTCGG
TTCTTCGACAGCATGTGTCTGGAGTCCTTCACACAGGGGCTGCCACTGTCCAATTTCGTG
CCACCCCCACCCTCGCCCGCCCCATCTGACTCGCCAGCGTCCCCGGATGAGGACCTGCAG
GCCTGGAACGTCTCCCTGCCGCCCACCGCTGGGCCAGAAATGTGGACGTCGGCACCCTCC
CTGCCGCGCCTGGTACGGGAGCCCGTCCGCTGCACCTGCTCTGCGCAGGGCACCGGCTTC
TCCTGCCCCAGCAGTGTGGGCGGGCACCCGCCCCAGATGCGGGTGGTCACAGGCGACATC
CTGACCGACATCACCGGCCACAATGTCTCTGAGTACCTGCTCTTCACCTCCGACCGCTTC
CGACTGCACCGGTATGGGGCCATCACCTTTGGAAACGTCCTGAAGTCCATCCCAGCCTCA
TTTGGCACCAGGGCCCCACCCATGGTGCGGAAGATCGCGGTGCGCAGGGCTGCCCAGGTT
TTCTACAACAACAAGGGCTATCACAGCATGCCCACCTACCTCAACAGCCTCAACAACGCC
ATCCTGCGTGCCAACCTGCCCAAGAGCAAGGGCAACCCGGCGGCTTACGGCATCACCGTC
ACCAACCACCCCATGAATAAGACCAGCGCCAGCCTCTCCCTGGATTACCTGCTGCAGGGC
ACGGATGTCGTCATCGCCATCTTCATCATCGTGGCCATGTCCTTCGTGCCGGCCAGCTTC
GTTGTCTTCCTCGTGGCCGAGAAGTCCACCAAGGCCAAGCACCTGCAGTTTGTCAGCGGC
TGCAACCCCATCATCTACTGGCTGGCGAACTACGTGTGGGACATGCTCAACTACCTGGTC
CCCGCTACCTGCTGTGTCATCATCCTGTTTGTGTTCGACCTGCCGGCCTACACGTCGCCC
ACCAACTTCCCTGCCGTCCTCTCCCTCTTCCTGCTCTATGGGTGGTCCATCACGCCCATC
ATGTACCCGGCCTCCTTCTGGTTCGAGGTCCCCAGCTCCGCCTACGTGTTCCTCATTGTC
ATCAATCTCTTCATCGGCATCACCGCCACCGTGGCCACCTTCCTGCTACAGCTCTTCGAG
CACGACAAGGACCTGAAGGTTGTCAACAGTTACCTGAAAAGCTGCTTCCTCATTTTCCCC
AACTACAACCTGGGCCACGGGCTCATGGAGATGGCCTACAACGAGTACATCAACGAGTAC
TACGCCAAGATTGGCCAGTTTGACAAGATGAAGTCCCCGTTCGAGTGGGACATTGTCACC
CGCGGACTGGTGGCCATGGCGGTTGAGGGCGTCGTGGGCTTCCTCCTGACCATCATGTGC
CAGTACAACTTCCTGCGGCGGCCACAGCGCATGCCTGTGTCTACCAAGCCTGTGGAGGAT
GATGTGGACGTGGCCAGTGAGCGGCAGCGAGTGCTCCGGGGAGACGCCGACAATGACATG
GTCAAGATTGAGAACCTGACCAAGGTCTACAAGTCCCGGAAGATTGGCCGTATCCTGGCC
GTTGACCGCCTGTGCCTGGGTGTGCGTCCTGGCGAGTGCTTCGGGCTCCTGGGCGTCAAC
GGTGCGGGCAAGACCAGCACCTTCAAGATGCTGACCGGCGACGAGAGCACGACGGGGGGC
GAGGCCTTCGTCAATGGACACAGCGTGCTGAAGGAGCTGCTCCAGGTGCAGCAGAGCCTC
GGCTACTGCCCGCAGTGTGACGCGCTGTTCGACGAGCTCACGGCCCGGGAGCACCTGCAG
CTGTACACGCGGCTGCGTGGGATCTCCTGGAAGGACGAGGCCCGGGTGGTGAAGTGGGCT
CTGGAGAAGCTGGAGCTGACCAAGTACGCAGACAAGCCGGCTGGCACCTACAGCGGCGGC
AACAAGCGGAAGCTCTCCACGGCCATCGCCCTCATTGGGTACCCAGCCTTCATCTTCCTG
GACGAGCCCACCACAGGCATGGACCCCAAGGCCCGGCGCTTCCTCTGGAACCTCATCCTT
GACCTCATCAAGACAGGGCGTTCAGTGGTGCTGACATCACACAGCATGGAGGAGTGCGAG
GCGCTGTGCACGCGGCTGGCCATCATGGTGAACGGTCGCCTGCGGTGCCTGGGCAGCATC
CAGCACCTGAAGAACCGGTTTGGAGATGGCTACATGATCACGGTGCGGACCAAGAGCAGC
CAGAGTGTGAAGGACGTGGTGCGGTTCTTCAACCGCAACTTCCCGGAAGCCATGCTCAAG
GAGCGGCACCACACAAAGGTGCAGTACCAGCTCAAGTCGGAGCACATCTCGCTGGCCCAG
GTGTTCAGCAAGATGGAGCAGGTGTCTGGCGTGCTGGGCATCGAGGACTACTCGGTCAGC
CAGACCACACTGGACAATGTGTTCGTGAACTTTGCCAAGAAGCAGAGTGACAACCTGGAG
CAGCAGGAGACGGAGCCGCCATCCGCACTGCAGTCCCCTCTCGGCTGCTTGCTCAGCCTG
CTCCGGCCCCGGTCTGCCCCCACGGAGCTCCGGGCACTTGTGGCAGACGAGCCCGAGGAC
CTGGACACGGAGGACGAGGGCCTCATCAGCTTCGAGGAGGAGCGGGCCCAGCTGTCCTTC
AACACGGACACGCTCTGCTTG
Protein sequence predicted based on nucleotide sequence
MGRKTSRVQQGPPRSPACSAHGERSWGLEFPPFRWLLGIAGRTHLAALPPFQWVLAFEIF IPLVLFFILLGLRQKKPTISVKEVSFYTAAPLTSAGILPVMQSLCPDGQRDEFGFLQYAN STVTQLLERLDRVVEEGNLFDPARPSLGSELEALRQHLEALSAGPGTSGSHLDRSTVSSF SLDSVARNPQELWRFLTQNLSLPNSTAQALLAARVDPPEVYHLLFGPSSALDSQSGLHKG QEPWSRLGGNPLFRMEELLLAPALLEQLTCTPGSGELGRILTVPESQKGALQGYRDAVCS GQAAARARRFSGLSAELRNQLDVAKVSQQLGLDAPNGSDSSPQAPPPRRLQALLGDLLDA QKVLQDVDVLSALALLLPQGACTGRTPGPPASGAGGAANGTGAGAVMGPNATAEEGAPSA AALATPDTLQGQCSAFVQLWAGLQPILCGNNRTIEPEALRRGNMSSLGFTSKEQRNLGLL VHLMTSNPKILYAPAGSEVDRVILKANETFAFVGNVTHYAQVWLNISAEIRSFLEQGRLQ QHLRWLQQYVAELRLHPEALNLSLDELPPALRQDNFSLPSGMALLQQLDTIDNAACGWIQ FMSKVSVDIFKGFPDEESIVNYTLNQAYQDNVTVFASVIFQTRKDGSLPPHVHYKIRQNS SFTEKTNEIRRAYWRPGPNTGGRFYFLYGFVWIQDMMERAIIDTFVGHDVVEPGSYVQMF PYPCYTRDDFLFVIEHMMPLCMVISWVYSVAMTIQHIVAEKEHRLKEVMKTMGLNNAVHW VAWFITGFVQLSISVTALTAILKYGQVLMHSHVVIIWLFLAVYAVATIMFCFLVSVLYSK AKLASACGGIIYFLSYVPYMYVAIREEVAHDKITAFEKCIASLMSTTAFGLGSKYFALYE VAGVGIQWHTFSQSPVEGDDFNLLLAVTMLMVDAVVYGILTWYIEAVHPGMYGLPRPWYF PLQKSYWLGSGRTEAWEWSWPWARTPRLSVMEEDQACAMESRRFEETRGMEEEPTHLPLV VCVDKLTKVYKDDKKLALNKLSLNLYENQVVSFLGHNGAGKTTTMSILTGLFPPTSGSAT IYGHDIRTEMDEIRKNLGMCPQHNVLFDRLTVEEHLWFYSRLKSMAQEEIRREMDKMIED LELSNKRHSLVQTLSGGMKRKLSVAIAFVGGSRAIILDEPTAGVDPYARRAIWDLILKYK PGRTILLSTHHMDEADLLGDRIAIISHGKLKCCGSPLFLKGTYGDGYRLTLVKRPAEPGG PQEPGLASSPPGRAPLSSCSELQVSQFIRKHVASCLLVSDTSTELSYILPSEAAKKGAFE RLFQHLERSLDALHLSSFGLMDTTLEEVFLKVSEEDQSLENSEADVKESRKDVLPGAEGP ASGEGHAGNLARCSELTQSQASLQSASSVGSARGDEGAGYTDVYGDYRPLFDNPQDPDNV SLQEVEAEALSRVGQGSRKLDGGWLKVRQFHGLLVKRFHCARRNSKALFSQILLPAFFVC VAMTVALSVPEIGDLPPLVLSPSQYHNYTQPRGNFIPYANEERREYRLRLSPDASPQQLV STFRLPSGVGATCVLKSPANGSLGPTLNLSSGESRLLAARFFDSMCLESFTQGLPLSNFV PPPPSPAPSDSPASPDEDLQAWNVSLPPTAGPEMWTSAPSLPRLVREPVRCTCSAQGTGF SCPSSVGGHPPQMRVVTGDILTDITGHNVSEYLLFTSDRFRLHRYGAITFGNVLKSIPAS FGTRAPPMVRKIAVRRAAQVFYNNKGYHSMPTYLNSLNNAILRANLPKSKGNPAAYGITV TNHPMNKTSASLSLDYLLQGTDVVIAIFIIVAMSFVPASFVVFLVAEKSTKAKHLQFVSG CNPIIYWLANYVWDMLNYLVPATCCVIILFVFDLPAYTSPTNFPAVLSLFLLYGWSITPI MYPASFWFEVPSSAYVFLIVINLFIGITATVATFLLQLFEHDKDLKVVNSYLKSCFLIFP NYNLGHGLMEMAYNEYINEYYAKIGQFDKMKSPFEWDIVTRGLVAMAVEGVVGFLLTIMC QYNFLRRPQRMPVSTKPVEDDVDVASERQRVLRGDADNDMVKIENLTKVYKSRKIGRILA VDRLCLGVRPGECFGLLGVNGAGKTSTFKMLTGDESTTGGEAFVNGHSVLKELLQVQQSL GYCPQCDALFDELTAREHLQLYTRLRGISWKDEARVVKWALEKLELTKYADKPAGTYSGG NKRKLSTAIALIGYPAFIFLDEPTTGMDPKARRFLWNLILDLIKTGRSVVLTSHSMEECE ALCTRLAIMVNGRLRCLGSIQHLKNRFGDGYMITVRTKSSQSVKDVVRFFNRNFPEAMLK ERHHTKVQYQLKSEHISLAQVFSKMEQVSGVLGIEDYSVSQTTLDNVFVNFAKKQSDNLE QQETEPPSALQSPLGCLLSLLRPRSAPTELRALVADEPEDLDTEDEGLISFEEERAQLSF NTDTLCL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
