Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00859960
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 6177nts
Open reading frame: 1 to 6177
ATGTTTAACCTAATGAAGAAAGACAAGGACAAAGATGGCGGGCGGAAGGAGAAGAAGGAG
AAAAAGGAGAAAAAGGAGCGGATGTCAGCGGCAGAGCTTCGGAGCCTGGAGGAGATGAGC
CTGCGACGTGGCTTCTTCAACCTGAACCGCTCCTCCAAGCGTGAATCCAAGACGCGCCTG
GAAATCTCCAACCCCATCCCCATCAAGGTGGCCAGCGGCTCTGACCTGCACCTGACTGAC
ATTGACTCCGATAGTAACCGGGGCAGCGTCATCCTGGACTCGGGCCACCTAAGTACAGCC
AGCTCCAGCGATGACCTCAAGGGTGAGGAGGGTAGCTTCCGTGGCTCGGTGCTGCAGCGG
GCAGCCAAGTTCGGCTCACTGGCCAAGCAGAACTCACAGATGATTGTCAAGCGCTTTTCC
TTCTCCCAGCGTAGCCGGGATGAGAGCGCCTCAGAAACCTCGACGCCCTCAGAGCACTCT
GCCGCCCCCTCGCCACAGGTGGAGGTGAGGACTCTAGAGGGACAGCTGGTGCAGCATCCT
GGCCCAGGCATCCCTCGACCAGGGCACCGATCCCGAGCCCCTGAGCTAGTGACTAAAAAG
TTCCCAGTCGACCTGCGCCTGCCCCCCGTGGTGCCCCTGCCCCCACCTACCCTCCGGGAG
CTGGAGCTGCAACGACGGCCCACTGGAGACTTTGGCTTCTCCCTGCGGCGCACAACCATG
CTGGATCGGGGCCCCGAGGGCCAGGCCTGTCGGCGTGTGGTCCACTTTGCTGAGCCTGGT
GCAGGCACCAAGGACCTGGCCCTGGGGCTGGTGCCAGGAGATCGACTGGTGGAGATTAAT
GGGCACAATGTGGAGAGCAAGTCCAGGGATGAGATTGTGGAGATGATCCGGCAGTCAGGG
GACAGCGTGCGGCTCAAGGTGCAGCCCATTCCAGAGCTCAGCGAGCTCAGCAGGAGCTGG
CTGCGGAGCGGCGAGGGACCTCGCAGGGAGCCATCCGATCTGGACTCTGAGGCCGCCTCT
CATGCCCACAGCCAGGCGAAAACAGAAGAACAGATTGCAGCAGAAGAGGCCTGGAATGAG
ACGGAGAAGGTGTGGCTGGTCCATAGGGACGGCTTCTCACTGGCCAGTCAACTCAAATCT
GAGGAGCTCAACTTGCCTGAGGGGAAGGTGCGTGTGAAGCTGGACCACGATGGGGCCATC
CTGGATGTGGATGAGGATGACGTTGAGAAGGCTAATGCTCCCTCCTGCGACCGTCTGGAG
GATCTGGCCTCACTGGTGTACCTCAATGAGTCCAGCGTCCTGCACACCTTGCGCCAGCGC
TATGGCGCTAGCCTGCTGCACACGTATGCTGGCCCCAGCCTGCTGGTTCTTGGCCCCCGT
GGGGCCCCTGCTGTGTACTCTGAGAAGGTGATGCACATGTTCAAGGGTTGTCGGCGGGAG
GACATGGCACCCCACATCTATGCAGTGGCCCAGACCGCATACAGGGCGATGCTGATGAGC
CGTCAGGATCAGTCAATCATCCTCCTGGGCAGTAGTGGCAGTGGCAAGACCACCAGCTGC
CAGCATCTGGTGCAGTACCTGGCCACCATCGCGGGCATCAGCGGGAACAAGGTGTTTTCT
GTGGAGAAGTGGCAGGCTCTGTACACCCTCCTGGAAGCCTTTGGGAACAGCCCCACCATC
ATTAATGGCAATGCCACCCGCTTCTCCCAGATCCTCTCCCTGGACTTTGACCAAGCTGGC
CAGGTGGCCTCAGCCTCCATTCAGACAATGCTTCTGGAGAAGCTGCGTGTGGCTCGGCGC
CCAGCCAGTGAAGCCACATTCAACGTCTTCTACTACCTGCTGGCCTGTGGGGATGGCACC
CTCAGGACAGAGCTCCACCTCAACCACTTGGCAGAGAACAATGTGTTTGGGATTGTGCCA
CTGGCCAAGCCTGAGGAAAAGCAGAAGGCAGCTCAGCAGTTTAGTAAGCTGCAGGCGGCC
ATGAAGGTGCTGGGCATCTCCCCCGATGAACAGAAGGCCTGCTGGTTCATTCTGGCTGCC
ATCTACCACCTGGGGGCTGCGGGAGCCACCAAAGAGGCCCCCGAGGAGCAAGCGGAAGCT
GCTGAAGCTGGGCGCAAGCAGTTTGCCCGCCATGAGTGGGCCCAGAAGGCTGCGTACCTA
CTGGGCTGCAGCCTGGAGGAGCTGTCCTCAGCCATCTTCAAGCACCAGCACAAGGGTGGC
ACCCTGCAGCGCTCCACCTCCTTCCGCCAGGGCCCCGAGGAGAGTGGCCTGGGAGATGGG
ACAGGCCCGAAACTGAGTGCACTGGAGTGCCTTGAGGGCATGGCGGCCGGCCTCTACAGC
GAGCTCTTCACCCTTCTCGTCTCCCTGGTGAATAGGGCTCTCAAGTCCAGCCAGCACTCA
CTCTGCTCCATGATGATTGTCGACACCCCGGGCTTCCAGAACCCTGAGCAGGGTGGGTCA
GCCCGCGGAGCCTCCTTTGAGGAGCTGTGCCACAACTACACCCAAGACCGGCTGCAGAGG
CTCTTCCACGAGCGCACCTTCGTGCAGGAGTTGGAAAGATACAAGGAGGAGAACATCGAG
CTGGCGTTTGACGACTTGGAACCCCCGACGGATGACTCTGTGGCTGCTGTGGACCAGGCC
TCCCATCAGTCCCTGGTCCGCTCGCTGGCCCGCACAGACGAGGCGAGGGGCCTGCTCTGG
CTATTGGAAGAGGAGGCTCTGGTGCCAGGGGCCAGTGAGGACACCCTCCTGGAGCGCCTT
TTCTCCTATTATGGCCCCCAGGAAGGTGACAAAAAAGGCCAAAGCCCCCTTCTGCACAGC
AGCAAACCACACCACTTTCTCCTGGGCCACAGCCATGGCACCAACTGGGTAGAGTACAAT
GTGACTGGCTGGCTGAACTACACCAAGCAGAACCCAGCCACCCAGAATGCCCCCCGGCTC
CTGCAGGACTCCCAGAAAAAAATCATCAGCAACCTGTTTCTGGGCCGCGCAGGCAGTGCC
ACGGTGCTCTCTGGCTCCATCGCGGGCCTGGAGGGCGGCTCGCAGCTGGCACTGCGCCGG
GCCACCAGCATGCGGAAAACCTTTACCACAGGCATGGCGGCTGTCAAAAAGAAGTCACTG
TGCATCCAGATGAAGCTACAGGTGGACGCCCTCATCGACACCATCAAGAAGTCAAAGCTG
CATTTTGTGCACTGCTTCCTGCCTGTAGCTGAGGGCTGGGCTGGGGAGCCCCGTTCCGCC
TCCTCCCGCCGAGTCAGCAGCAGCAGTGAGCTGGACCTGCCCTCGGGAGACCACTGCGAG
GCTGGGCTCCTGCAGCTCGACGTGCCCCTGCTCCGCACCCAGCTCCGCGGCTCCCGCCTG
CTCGATGCCATGCGCATGTACCGCCAAGGTTACCCTGACCACATGGTGTTTTCCGAGTTC
CGCCGCCGCTTTGATGTCCTGGCCCCGCACCTGACCAAGAAACACGGGCGTAACTACATC
GTGGTGGATGAAAGGCGGGCAGTGGAGGAGCTGCTGGAGTGCTTGGATCTGGAGAAGAGC
AGCTGCTGCATGGGCCTGAGCCGGGTGTTCTTCCGGGCGGGCACCTTGGCACGGCTGGAG
GAGCAGCGGGATGAACAAACCAGCAGGAACCTAACCCTGTTCCAAGCAGCCTGCAGGGGC
TACCTGGCCCGCCAGCACTTCAAGAAGAGAAAGATCCAGGACCTGGCCATTCGCTGTGTA
CAGAAGAACATCAAGAAGAACAAAGGGGTGAAGGACTGGCCCTGGTGGAAGCTTTTTACC
ACAGTGAGGCCCCTCATCGAAGTACAGCTGTCAGAGGAGCAGATCCGGAACAAAGACGAG
GAGATCCAGCAGCTGCGGAGCAAGCTCGAGAAGGCGGAGAAGGAGAGGAACGAGCTGCGG
CTCAACAGTGACCGGCTGGAGAGCCGGATCTCAGAGCTGACATCGGAGCTGACAGATGAG
CGTAACACAGGAGAGTCCGCCTCCCAGCTGCTGGACGCGGAGACAGCAGAGAGGCTCCGG
GCTGAGAAGGAGATGAAGGAACTGCAGACCCAGTACGATGCACTGAAGAAGCAGATGGAG
GTTATGGAAATGGAGGTGATGGAGGCCCGTCTCATCCGGGCAGCGGAGATCAACGGGGAA
GTGGATGATGATGATGCAGGTGGCGAGTGGCGGCTGAAGTATGAGCGGGCTGTGCGGGAG
GTGGACTTCACCAAGAAACGGCTCCAGCAGGAGTTTGAGGACAAGCTGGAGGTGGAGCAG
CAGAACAAGAGGCAGCTGGAACGGCGGCTCGGGGACCTGCAGGCAGATAGTGAGGAGAGT
CAGCGGGCTCTGCAGCAGCTCAAGAAGAAGTGCCAGCGACTGACGGCTGAGCTGCAAGAC
ACCAAGCTGCACCTGGAGGGCCAGCAGGTCCGCAACCACGAACTGGAGAAGAAGCAGAGG
AGGTTTGACAGTGAGCTCTCGCAGGCGCATGAGGAGGCCCAGCGGGAGAAGCTGCAGCGG
GAGAAGCTGCAGCGGGAGAAGGACATGCTCCTCGCTGAGGCTTTCAGCCTGAAGCAGCAA
CTAGAGGAAAAAGACATGGACATTGCAGGGTTCACCCAGAAGGTTGTGTCTCTAGAGGCA
GAGCTCCAGGACATTTCTTCCCAAGAGTCCAAGGATGAGGCTTCTCTGGCCAAGGTCAAG
AAACAGCTCCGGGACCTGGAGGCCAAAGTCAAGGATCAGGAAGAAGAGCTGGATGAGCAG
GCAGGGACCATCCAGATGCTGGAACAGGCCAAGCTGCGTCTGGAGATGGAGATGGAGCGG
ATGAGACAGACCCATTCTAAGGAGATGGAGAGTCGGGATGAGGAGGTGGAGGAGGCCCGG
CAGTCGTGTCAGAAGAAGTTAAAACAGATGGAGGTGCAGCTAGAGGAAGAGTATGAGGAC
AAGCAGAAGGTTCTGCGAGAGAAGCGGGAGCTGGAGGGCAAGCTCGCCACCCTCAGCGAC
CAGGTGAACCGGCGGGACTTTGAGTCAGAGAAGCGGCTGCGGAAGGACCTGAAGCGCACC
AAGGCCCTGCTGGCAGATGCCCAGCTCATGCTGGACCACCTGAAGAACAGTGCTCCCAGC
AAGCGAGAGATTGCCCAGCTCAAGAACCAGCTGGAGGAGTCAGAGTTCACCTGTGCGGCA
GCCGTGAAAGCACGGAAAGCAATGGAGGTGGAGATCGAAGACCTGCACCTGCAGATTGAT
GACATCGCCAAAGCCAAGACAGCGCTGGAGGAGCAGCTGAGCCGCCTTCAGCGTGAGAAG
AATGAGATCCAGAACCGGCTGGAGGAAGATCAGGAAGACATGAACGAATTGATGAAGAAG
CACAAGGCTGCCGTGGCTCAGGCTTCCCGGGACCTGGCTCAGATAAATGATCTCCAAGCT
CAGCTAGAAGAAGCCAACAAAGAGAAGCAGGAGCTGCAGGAGAAGCTACAAGCCCTCCAG
AGCCAGGTGGAGTTCCTGGAGCAGTCCATGGTGGACAAGTCCCTGGTGAGCAGGCAGGAA
GCTAAGATACGGGAGCTGGAGACACGCCTGGAGTTTGAAAGGACGCAAGTGAAACGGCTG
GAGAGCCTGGCTAGCCGTCTCAAGGAAAACATGGAGAAGCTGACTGAGGAGCGGGATCAG
CGCATTGCAGCCGAGAACCGGGAGAAGGAACAGAACAAGCGGCTACAGAGGCAGCTCCGG
GACACCAAGGAGGAGATGGGCGAGCTTGCCAGGAAGGAGGCCGAGGCGAGCCGCAAGAAG
CACGAACTGGAGATGGATCTAGAAAGCCTGGAGGCTGCTAACCAGAGCCTGCAGGCTGAC
CTAAAGTTGGCATTCAAGCGCATCGGGGACCTGCAGGCTGCCATTGAGGATGAGATGGAG
AGTGATGAGAATGAGGACCTCATCAACAGTGAGGGAGACTCTGATGTGGACTCGGAGCTG
GAGGACCGTGTTGACGGGGTCAAGTCCTGGTTGTCAAAAAACAAGGGACCTTCCAAGGCA
GCTTCTGATGATGGCAGCTTAAAGAGTTCCAGCCCCACCAGCTACTGGAAGTCCCTTGCC
CCTGATCGGTCAGATGATGAGCACGACCCTCTCGACAACACCTCCAGACCGCGATACTCC
CACAGTTATCTGAGTGACAGCGACACAGAGGCCAAGCTGACGGAGACTAACGCATTG
Protein sequence predicted based on nucleotide sequence
MFNLMKKDKDKDGGRKEKKEKKEKKERMSAAELRSLEEMSLRRGFFNLNRSSKRESKTRL EISNPIPIKVASGSDLHLTDIDSDSNRGSVILDSGHLSTASSSDDLKGEEGSFRGSVLQR AAKFGSLAKQNSQMIVKRFSFSQRSRDESASETSTPSEHSAAPSPQVEVRTLEGQLVQHP GPGIPRPGHRSRAPELVTKKFPVDLRLPPVVPLPPPTLRELELQRRPTGDFGFSLRRTTM LDRGPEGQACRRVVHFAEPGAGTKDLALGLVPGDRLVEINGHNVESKSRDEIVEMIRQSG DSVRLKVQPIPELSELSRSWLRSGEGPRREPSDLDSEAASHAHSQAKTEEQIAAEEAWNE TEKVWLVHRDGFSLASQLKSEELNLPEGKVRVKLDHDGAILDVDEDDVEKANAPSCDRLE DLASLVYLNESSVLHTLRQRYGASLLHTYAGPSLLVLGPRGAPAVYSEKVMHMFKGCRRE DMAPHIYAVAQTAYRAMLMSRQDQSIILLGSSGSGKTTSCQHLVQYLATIAGISGNKVFS VEKWQALYTLLEAFGNSPTIINGNATRFSQILSLDFDQAGQVASASIQTMLLEKLRVARR PASEATFNVFYYLLACGDGTLRTELHLNHLAENNVFGIVPLAKPEEKQKAAQQFSKLQAA MKVLGISPDEQKACWFILAAIYHLGAAGATKEAPEEQAEAAEAGRKQFARHEWAQKAAYL LGCSLEELSSAIFKHQHKGGTLQRSTSFRQGPEESGLGDGTGPKLSALECLEGMAAGLYS ELFTLLVSLVNRALKSSQHSLCSMMIVDTPGFQNPEQGGSARGASFEELCHNYTQDRLQR LFHERTFVQELERYKEENIELAFDDLEPPTDDSVAAVDQASHQSLVRSLARTDEARGLLW LLEEEALVPGASEDTLLERLFSYYGPQEGDKKGQSPLLHSSKPHHFLLGHSHGTNWVEYN VTGWLNYTKQNPATQNAPRLLQDSQKKIISNLFLGRAGSATVLSGSIAGLEGGSQLALRR ATSMRKTFTTGMAAVKKKSLCIQMKLQVDALIDTIKKSKLHFVHCFLPVAEGWAGEPRSA SSRRVSSSSELDLPSGDHCEAGLLQLDVPLLRTQLRGSRLLDAMRMYRQGYPDHMVFSEF RRRFDVLAPHLTKKHGRNYIVVDERRAVEELLECLDLEKSSCCMGLSRVFFRAGTLARLE EQRDEQTSRNLTLFQAACRGYLARQHFKKRKIQDLAIRCVQKNIKKNKGVKDWPWWKLFT TVRPLIEVQLSEEQIRNKDEEIQQLRSKLEKAEKERNELRLNSDRLESRISELTSELTDE RNTGESASQLLDAETAERLRAEKEMKELQTQYDALKKQMEVMEMEVMEARLIRAAEINGE VDDDDAGGEWRLKYERAVREVDFTKKRLQQEFEDKLEVEQQNKRQLERRLGDLQADSEES QRALQQLKKKCQRLTAELQDTKLHLEGQQVRNHELEKKQRRFDSELSQAHEEAQREKLQR EKLQREKDMLLAEAFSLKQQLEEKDMDIAGFTQKVVSLEAELQDISSQESKDEASLAKVK KQLRDLEAKVKDQEEELDEQAGTIQMLEQAKLRLEMEMERMRQTHSKEMESRDEEVEEAR QSCQKKLKQMEVQLEEEYEDKQKVLREKRELEGKLATLSDQVNRRDFESEKRLRKDLKRT KALLADAQLMLDHLKNSAPSKREIAQLKNQLEESEFTCAAAVKARKAMEVEIEDLHLQID DIAKAKTALEEQLSRLQREKNEIQNRLEEDQEDMNELMKKHKAAVAQASRDLAQINDLQA QLEEANKEKQELQEKLQALQSQVEFLEQSMVDKSLVSRQEAKIRELETRLEFERTQVKRL ESLASRLKENMEKLTEERDQRIAAENREKEQNKRLQRQLRDTKEEMGELARKEAEASRKK HELEMDLESLEAANQSLQADLKLAFKRIGDLQAAIEDEMESDENEDLINSEGDSDVDSEL EDRVDGVKSWLSKNKGPSKAASDDGSLKSSSPTSYWKSLAPDRSDDEHDPLDNTSRPRYS HSYLSDSDTEAKLTETNAL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
