Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860173
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 7221nts
Open reading frame: 1 to 7221
ATGGATCCTGAACAAAGTGTCAAGGGCACCAAGAAGGCTGAGGGAAGTCCCCGGAAGCGG
CTGACCAAAGGAGAGGCCATTCAGACCAGTGTTTCTTCCAGCGTCCCATACCCAGGCAGC
GGCACAGCTGCCACCCAAGAGAGCCCCGCCCAAGAGCTCTTAGCCCCGCAGCCCTTCCCG
GGCCCCTCATCAGTTCTTAGGGAAGGCTCTCAGGAGAAAACGGGCCAGCAGCAGAAGCCC
CCCAAAAGGCCCCCCATCGAAGCATCCGTCCACATCTCACAGCTTCCGCAGCACCCTCTG
ACACCAGCATTCATGTCGCCTGGCAAACCTGAGCATCTCCTGGAGGGGTCCACATGGCAA
CTGGTTGACCCCATGAGACCTGGACCCTCTGGCTCCTTCGTGGCCCCTGGGCTCCATCCT
CAGAGCCAGCTCCTTCCTTCCCACGCTTCCATCATTCCCCCCGAGGACCTTCCTGGAGTC
CCCAAAGTCTTCGTGCCTCGTCCTTCCCAGGTCTCCTTGAAGCCCACAGAAGAGGCACAC
AAGAAGGAGAGGAAGCCCCAGAAGCCAGGCAAGTACATCTGCCAGTACTGCAGCCGGCCC
TGTGCCAAGCCCAGCGTGCTCCAGAAGCACATTCGCTCACACACAGGTGAGAGGCCCTAC
CCCTGCGGCCCCTGTGGCTTCTCCTTCAAGACCAAGAGTAATCTCTACAAGCACAGGAAG
TCCCATGCCCACCGCATCAAAGCAGGCCTGGCCTCAGGCATGGGTGGCGAGATGTACCCA
CATGGGCTGGAGATGGAGCGGATCCCTGGGGAAGAGTTTGAGGAGCCCACTGAGGGAGAA
AGCACAGATTCTGAAGAGGAGACTAGTGCCACCTCTGGTCACCCTGCAGAGCTCTCCCCA
AGACCCAAGCAGCCCCTTCTCTCCAGCGGGCTATACAGCTCTGGGAGCCACAGTTCCAGC
CACGAACGCTGTTCCCTGTCCCAGTCCAGCACAGCCCAGTCACTCGAAGACCCCCCTCCA
TTTGTGGAACCCTCATCTGAGCACCCCCTGAGCCATAAACCTGAAGACACCCACACGATT
AAGCAGAAGCTGGCCCTCCGCTTAAGCGAGAGGAAGAAGGTGATCGATGAGCAGGCGTTT
CTGAGCCCAGGCAGCAAAGGGAGTACTGAGTCTGGGTATTTCTCTCGCTCCGAGAGTGCA
GAGCAGCAGGTCAGCCCCCCAAACACCAACGCCAAGTCCTACGCTGAGATCATCTTTGGC
AAGTGTGGGCGAATAGGACAGCGGACCGCCATGCTGACAGCCACCTCCACCCAGCCCCTC
CTGCCCCTGTCCACCGAAGACAAGCCCAGCCTGGTGCCTTTGTCTGTACCCCGGACGCAG
GTGATCGAGCACATCACGAAGCTCATCACCATCAACGAGGCCGTGGTGGACACCAGCGAG
ATCGACAGCGTGAAGCCAAGGCGGAGCTCACTGTCCAGGCGCAGCAGCATGGAGTCCCCA
AAATCCAGCCTCTACCGGGAGCCCCTGTCATCCCACAGTGAGAAAACCAAGCCTGAACAA
TCACTGCTGAGCCTCCAGCACCCGCCCAGTACCGCCCCCCCTGTGCCTCTCCTGAGAAGC
CACTCAATGCCTTCTGCCGCCTGCACTATCAGCACCCCCCACCACCCCTTCCGAGGTAGC
TACTCCTTCGATGACCATATCACCGACTCCGAAGCCCTGAGCCACAGCAGTCACGTGTTT
ACCTCCCACCCCCGGATGCTGAAGCGCCAGCCGGCAATCGAATTACCTTTGGGAGGGGAA
TACAGTTCTGAGGAGCCTGGCCCAAGCAGCAAAGACACAGCCTCCAAGCCCTCGGACGAA
GTGGAACCCAAGGAAAGCGAGCTTACCAAAAAGACCAAGAAGGGTTTGAAAACAAAAGGG
GTGATCTACGAATGTAACATATGTGGTGCTCGGTACAAGAAAAGGGATAACTACGAAGCC
CACAAAAAATACTACTGCTCAGAGCTTCAGATCGCAAAGCCCATCTCTGCAGGCACCCAC
ACATCTCCAGAAGCTGAAAAGAGTCAGATTGAGCATGAGCCGTGGTCCCAAATGATGCAT
TACAAACTGGGAACCACCCTGGAACTCACTCCACTGAGGAAGAGGAGGAAAGAGAAGAGC
CTTGGGGACGAGGAAGAGCCACCTGCCTTTGAGTCCACAAAAAGTCAGTTTGGCAGCCCC
GGGCCATCTGATGCTGCTCGGAACCTTCCCCTGGAGTCCACCAAGTCACCAGCAGAACCA
AGTAAATCAGTGCCCTCCTTGGAGGGACCCACGGGCTTCCAGCCAAGGACTCCCAAGCCA
GGGTCCGGTTCAGAATCAGGGAAGGAGAGGAGAACAACGTCCAAAGAAATTTCTGTCATC
CAGCACACCAGCTCCTTTGAGAAATCTGATTCTCTCGAGCAGCCGAGTGGCTTGGAAGGG
GAAGACAAACCTCTGGCCCAGTTCCCATCACCCCCACCTGCCCCACACGGACGCTCTGCT
CACTCCCTGCAGCCTAAGTTGGTCCGCCAGCCCAACATTCAGGTTCCTGAGATCCTAGTA
ACTGAGGAGCCTGACCGGCCGGACACAGAGCCAGAGCCGCCCCCTAAGGAACCTGAGAAG
ACTGAGGAGTTCCAATGGCCCCAGCGCAGCCAGACACTTGCCCAGCTCCCAGCTGAGAAG
CTGCCACCCAAAAAGAAGAGGTTGCGCCTGGCAGAGATGGCCCAATCATCAGGGGAGTCC
AGCTTCGAGTCCTCTGTGCCTCTGTCTCGCAGCCCGAGCCAGGAAAGCAATGTCTCTTTG
AGTGGGTCCAGCCGCTCAGCCTCGTTTGAGAGGGATGACCATGGGAAAGCCGAGGCCCCC
AGTCCCTCATCTGACATGCGCCCCAAACCCCTGGGCACCCACATGTTGACTGTCCCCAGC
CACCACCCACATGCCCGAGAGATGCGGAGGTCAGCCTCAGAGCAGAGCCCCAACGTTTCC
CATTCTGCCCACATGACCGAGACACGCAGCAAATCCTTTGACTATGGCAGCTTGTCCTTG
ACAGGCCCTTCTGCTCCAGCCCCAGTGGCTCCACCAGCGCGGGTGGCCCCGCCAGAGAGA
AGAAAATGCTTCTTGGTGAGACAGGCCTCTCTGAGCAGGCCTCCAGAATCTGAGTTGGAG
GTTGCCCCCAAGGGAAGACAGGAGAGCGAAGAACCACAGCCCTCATCCAGTAAACCCTCT
GCCAAAAGCTCATTGTCCCAGATTTCCTCTGCGGCCACCTCACATGGTGGACCCCCGGGA
GGCAAGGGCCCAGGGCAGGACAGGCCCCCATTGGGGCCCACTGTGCCCTACACAGAAGCA
CTGCAAGTGTTCCACCACCCCGTTGCCCAGACACCCCTGCATGAGAAGCCATACCTGCCC
CCACCAGTCTCCCTTTTCTCCTTCCAGCATCTCGTGCAGCATGAGCCAGGACAGTCTCCA
GAATTCTTCTCCACCCAGGCCATGTCCAGCCTCCTGTCCTCACCATACTCCATGCCCCCA
CTTCCTCCCTCCTTATTTCAAGCCCCACCGCTTCCTCTCCAGCCTACTGTTCTGCACCCA
GGCCAACTCCATCTCCCCCAGCTCATGCCTCACCCAGCCAACATCCCCTTCAGGCAGCCC
CCTTCCTTCCTCCCCATGCCATACCCGACCTCCTCAGCACTGTCTTCTGGGTTTTTCCTG
CCTCTGCAATCCCAGTTTGCACTTCAGCTCCCTGGTGATGTGGAAAGCCATCTGCCCCAG
ATCAAAACCAGCCTGGCCCCACTGGCAACAGGAAGTGCTGGCCTCTCCCCCAGCACAGAG
TACAGCAGTGACATCCGGCTACCCCCTGTGGCTCCCCCAGCCAGCTCCTCAGCACCTACA
TCAGCTCCTCCACTGGCCCTGCCTGCCTGTCCAGACACCATGGTGTCCCTGGTTGTGCCT
GTCCGTGTTCAGACCAATATGCCGTCCTATGGGAGCGCAATGTACACCACCCTTTCCCAG
ATCTTGGTCACCCAGTCCCAAGGCAGCTCAGCAACTGTGGCACTTCCCAAGTTTGAGGAA
CCCCCATCAAAGGGGACGACTGTATGTGGTGCAGATGTGCATGAGGTTGGGCCCGGCCCT
TCTGGGTTAAGTGAAGAGCAAAGCAGAGCTTTCCCAACTCCATACCTGAGAGTGCCTGTG
ACATTACCTGAAAGAAAAGGCACTTCCCTGTCATCAGAGAGTATCTTGAGCCTGGAGGGG
AGTTCATCAACAGCAGGGGGAAGCAAACGTGTCCTTTCACCAGCTGGCAGCCTTGAACTT
ACCATGGAAACCCAGCAGCAAAAAAGAGTGAAGGAGGAGGAGGCTTCCAAGGCAGATGAA
AAACTTGAGCTGGTAAAACCATGCAGTGTGGTCCTTACCAGCACCGAGGATGGGAAGAGG
CCAGAGAAATCCCACTTAGGCAACCAGGGCCAAGGCAGGAGGGAGCTAGAAATGCTGTCC
AGCCTGTCCTCAGATCCATCTGACACAAAGGAAATTCCTCCCCTCCCTCACCCTGCATTG
TCCCATGGGACAGCCCCAGGCTCAGAAGCTTTGAAGGAATATCCCCAGCCATCTGGCAAA
CCTCACCGAAGAGGGTTGACCCCACTGAGCGTGAAGAAAGAAGATTCCAAGGAACAACCT
GATCTCCCCTCCTTGGCACCTCCGAGCTCTCTGCCTCTGTCAGAAACGTCCTCCAGACCA
GCCAAGTCACAAGAAGGTACGGACTCAAAGAAGGTACTGCAGTTCCCCAGCCTCCACACA
ACCACTAATGTCAGTTGGTGCTATTTAAACTACATTAAGCCAAATCACATCCAGCATGCA
GATAGGAGGTCCTCTGTTTACGCTGGTTGGTGCATAAGTTTGTACAACCCCAACCTTCCG
GGGGTTTCCACTAAAGCTGCTTTGTCCCTCCTGAGGTCTAAGCAGAAAGTGAGCAAAGAG
ACATACACCATGGCCACAGCTCCGCATCCTGAGGCAGGAAGGCTTGTGCCATCCAGCTCC
CGCAAGCCCCGCATGACAGAGGTTCACCTCCCTTCACTGGTTTCCCCGGAAGGCCAGAAA
GATCTAGCTAGAGTGGAGAAGGAAGAAGAGAGGAGAGGGGAGCCGGAGGAGGATGCTCCT
GCCTCCCAGAGAGGGGAGCCGGCGAGGATCAAAATCTTCGAAGGAGGGTACAAATCAAAC
GAAGAGTATGTATATGTGCGAGGCCGCGGCCGAGGGAAATATGTTTGTGAGGAGTGTGGA
ATTCGCTGCAAGAAGCCCAGCATGCTGAAGAAACACATCCGCACCCACACTGACGTCCGG
CCCTATGTGTGCAAGCACTGTCACTTTGCTTTTAAAACCAAAGGGAATCTGACTAAGCAC
ATGAAGTCGAAGGCCCACAGCAAAAAGTGCCAAGAGACAGGGGTGCTGGAGGAGCTGGAA
GCCGAAGAAGGAACCAGTGACGACCTGTTCCAGGACTCGGAAGGACGAGAGGGTTCAGAG
GCTGTGGAGGAGCACCAGTTTTCGGACCTGGAGGACTCGGACTCAGACTCAGACCTGGAC
GAAGACGAGGATGAGGATGAGGAGGAGAGCCAGGATGAGCTGTCCAGACCATCCTCAGAG
GCGCCCCCGCCTGGCCCACCACATGCACTGCGGGCAGACTCCTCACCCATCCTGGGCCCT
CAGCCCCCAGATGCCCCCGCCTCTGGCACGGAGGCTACACGAGGCAGCTCGGTCTCGGAA
GCTGAGCGCCTGACAGCCAGCAGCTGCTCCATGTCCAGCCAGAGCATGCCGGGCCTCCCC
TGGCTGGGACCGGCCCCTCTGGGCTCTGTGGAGAAAGACACAGGCTCAGCCTTGAGCTAC
AAGCCTGTGTCCCCAAGAAGACCGTGGTCCCCAAGCAAAGAAGCAGGCAGCCGTCCACCA
CTAGCCCGCAAACACTCGCTAACCAAAAACGACTCATCTCCCCAGCGATGCTCCCCGGCC
CGAGAACCACAGGCCTCAGCCCCAAGCCCACCTGGCCTGCACGTGGACCCAGGAAGGGGC
ATGGGCGCTCTCCCTTGTGGGTCTCCAAGACTTCAGCTGTCTCCTCTCACCCTCTGCCCC
CTGGGAAGAGAACTGGCCCCTCGAGCACATGTGCTCTCCAAACTCGAGGGTACCACCGAC
CCAGGCCTCCCCAGATACTCGCCCACCAGGAGATGGTCTCCAGGTCAGGCCGAGTCACCA
CCACGGTCAGCGCCGCCAGGGAAGTGGGCCTTGGCTGGGCCGGGCAGCCCCTCAGCGGGG
GAGCATGGCCCAGGCTTGGGGCTGGACCCACGGGTTCTCTTCCCGCCCGCGCCTCTACCT
CACAAGCTCCTCAGCAGAAGCCCAGAGACCTGCGCCTCCCCGTGGCAGAAGGCCGAGTCC
CGAAGTCCCTCCTGCTCACCCGGCCCTGCTCATCCTCTCTCCTCCCGACCCTTCTCCGCC
CTCCATGACTTCCACGGCCACATCCTGGCCCGGACAGAGGAGAACATCTTCAGCCACCTG
CCTCTGCACTCCCAGCACTTGACCCGTGCCCCATGTCCCTTGATTCCCATCGGTGGGATC
CAGATGGTGCAGGCCCGGCCAGGAGCCCACCCCACCCTGCTGCCAGGGCCCACCGCAGCC
TGGGTCAGTGGCTTCTCCGGGGGTGGCAGCGACCTGACAGGGGCCCGGGAGGCCCAGGAG
CGAGGCCGCTGGAGTCCCACTGAGAGCTCGTCAGCCTCCGTGTCGCCTGTGGCTAAGGTC
TCCAAATTCACACTCTCCTCAGAGCTGGAGGGCGGGGACTACCCCAAGGAGAGGGAGAGG
ACCGGCGGAGGCCCGGGCAGGCCTCCTGACTGGACACCCCATGGGACCGGGGCACCTGCA
GAGCCCACACCCACGCACAGCCCCTGCACCCCACCCGACACCTTGCCCCGGCCGCCCCAG
GGACGCCGGGCAGCGCAGTCCTGGAGCCCCCGCTTGGAGTCCCCGCGTGCACCGACCAAC
CCCGAGCCTTCTGCCACCCCGCCGCTGGACCGCAGCAGCTCTGTGGGCTGCCTGGCAGAG
GCCTCTGCCCGCTTCCCAGCCCGGACGAGGAACCTCTCCGGGGAACCCAGGACCAGGCAG
GACTCCCCCAAGCCCTCAGGAAGTGGGGAGCCCAGGGCACATCCACATCAGCCTGAGGAC
AGGGTTCCCCCCAACGCTTTG
Protein sequence predicted based on nucleotide sequence
MDPEQSVKGTKKAEGSPRKRLTKGEAIQTSVSSSVPYPGSGTAATQESPAQELLAPQPFP GPSSVLREGSQEKTGQQQKPPKRPPIEASVHISQLPQHPLTPAFMSPGKPEHLLEGSTWQ LVDPMRPGPSGSFVAPGLHPQSQLLPSHASIIPPEDLPGVPKVFVPRPSQVSLKPTEEAH KKERKPQKPGKYICQYCSRPCAKPSVLQKHIRSHTGERPYPCGPCGFSFKTKSNLYKHRK SHAHRIKAGLASGMGGEMYPHGLEMERIPGEEFEEPTEGESTDSEEETSATSGHPAELSP RPKQPLLSSGLYSSGSHSSSHERCSLSQSSTAQSLEDPPPFVEPSSEHPLSHKPEDTHTI KQKLALRLSERKKVIDEQAFLSPGSKGSTESGYFSRSESAEQQVSPPNTNAKSYAEIIFG KCGRIGQRTAMLTATSTQPLLPLSTEDKPSLVPLSVPRTQVIEHITKLITINEAVVDTSE IDSVKPRRSSLSRRSSMESPKSSLYREPLSSHSEKTKPEQSLLSLQHPPSTAPPVPLLRS HSMPSAACTISTPHHPFRGSYSFDDHITDSEALSHSSHVFTSHPRMLKRQPAIELPLGGE YSSEEPGPSSKDTASKPSDEVEPKESELTKKTKKGLKTKGVIYECNICGARYKKRDNYEA HKKYYCSELQIAKPISAGTHTSPEAEKSQIEHEPWSQMMHYKLGTTLELTPLRKRRKEKS LGDEEEPPAFESTKSQFGSPGPSDAARNLPLESTKSPAEPSKSVPSLEGPTGFQPRTPKP GSGSESGKERRTTSKEISVIQHTSSFEKSDSLEQPSGLEGEDKPLAQFPSPPPAPHGRSA HSLQPKLVRQPNIQVPEILVTEEPDRPDTEPEPPPKEPEKTEEFQWPQRSQTLAQLPAEK LPPKKKRLRLAEMAQSSGESSFESSVPLSRSPSQESNVSLSGSSRSASFERDDHGKAEAP SPSSDMRPKPLGTHMLTVPSHHPHAREMRRSASEQSPNVSHSAHMTETRSKSFDYGSLSL TGPSAPAPVAPPARVAPPERRKCFLVRQASLSRPPESELEVAPKGRQESEEPQPSSSKPS AKSSLSQISSAATSHGGPPGGKGPGQDRPPLGPTVPYTEALQVFHHPVAQTPLHEKPYLP PPVSLFSFQHLVQHEPGQSPEFFSTQAMSSLLSSPYSMPPLPPSLFQAPPLPLQPTVLHP GQLHLPQLMPHPANIPFRQPPSFLPMPYPTSSALSSGFFLPLQSQFALQLPGDVESHLPQ IKTSLAPLATGSAGLSPSTEYSSDIRLPPVAPPASSSAPTSAPPLALPACPDTMVSLVVP VRVQTNMPSYGSAMYTTLSQILVTQSQGSSATVALPKFEEPPSKGTTVCGADVHEVGPGP SGLSEEQSRAFPTPYLRVPVTLPERKGTSLSSESILSLEGSSSTAGGSKRVLSPAGSLEL TMETQQQKRVKEEEASKADEKLELVKPCSVVLTSTEDGKRPEKSHLGNQGQGRRELEMLS SLSSDPSDTKEIPPLPHPALSHGTAPGSEALKEYPQPSGKPHRRGLTPLSVKKEDSKEQP DLPSLAPPSSLPLSETSSRPAKSQEGTDSKKVLQFPSLHTTTNVSWCYLNYIKPNHIQHA DRRSSVYAGWCISLYNPNLPGVSTKAALSLLRSKQKVSKETYTMATAPHPEAGRLVPSSS RKPRMTEVHLPSLVSPEGQKDLARVEKEEERRGEPEEDAPASQRGEPARIKIFEGGYKSN EEYVYVRGRGRGKYVCEECGIRCKKPSMLKKHIRTHTDVRPYVCKHCHFAFKTKGNLTKH MKSKAHSKKCQETGVLEELEAEEGTSDDLFQDSEGREGSEAVEEHQFSDLEDSDSDSDLD EDEDEDEEESQDELSRPSSEAPPPGPPHALRADSSPILGPQPPDAPASGTEATRGSSVSE AERLTASSCSMSSQSMPGLPWLGPAPLGSVEKDTGSALSYKPVSPRRPWSPSKEAGSRPP LARKHSLTKNDSSPQRCSPAREPQASAPSPPGLHVDPGRGMGALPCGSPRLQLSPLTLCP LGRELAPRAHVLSKLEGTTDPGLPRYSPTRRWSPGQAESPPRSAPPGKWALAGPGSPSAG EHGPGLGLDPRVLFPPAPLPHKLLSRSPETCASPWQKAESRSPSCSPGPAHPLSSRPFSA LHDFHGHILARTEENIFSHLPLHSQHLTRAPCPLIPIGGIQMVQARPGAHPTLLPGPTAA WVSGFSGGGSDLTGAREAQERGRWSPTESSSASVSPVAKVSKFTLSSELEGGDYPKERER TGGGPGRPPDWTPHGTGAPAEPTPTHSPCTPPDTLPRPPQGRRAAQSWSPRLESPRAPTN PEPSATPPLDRSSSVGCLAEASARFPARTRNLSGEPRTRQDSPKPSGSGEPRAHPHQPED RVPPNAL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
