Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860236
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4353nts
Open reading frame: 1 to 4353
ATGGCAGAGGCTGGGGATGCTGCGCTATCGGTGGCCGAGTGGCTGCGGGCATTGCACCTG
GAGCAGTACACGGGGCTCTTTGAGCAGCATGGCCTGGTGTGGGCCACTGAGTGCCAAGGC
CTCAGCGACACCCGCCTGATGGACATGGGCATGCTACTCCCTGGTCACCGCCGCCGCATC
CTGGCTGGCCTGCTCCGTGCCCATACCTCACCGGCCCCTGCACCCCGCCCCACCCCACGG
CCTGTGCCCATGAAGCGCCACATCTTCCGCTCACCACCTGTGCCTGCCACTCCACCCGAG
CCGCTGCCCACCACTACAGAGGATGAGGGGCTCCCCGCTGCCCCACCCATCCCGCCCCGG
AGGAGCTGCCTTCCGCCCACCTGCTTCACCACCCCATCCACAGCTGCCCCAGACCCTGTG
CTGCCCCCGCTGCCTGCTAAGCGGCATTTGGCAGAGCTGAGCGTTCCACCCGTGCCGCCC
CGCACCGGACCCCCCCGCCTGCTGGTGAGCCTGCCCACTAAGGAGGAGGAGTCATTGCTG
CCATCATTATCATCCCCTCCCCAGCCACAGTCTGAGGAGCCCCTGTCCACCCTCCCCCAG
GGGCCTCCCCAGCCTCCCTCTCCACCTCCCTGCCCCCCGGAGATACCTCCAAAGCCGGTA
CGCCTGTTCCCAGAGTTCGATGACTCTGACTACGATGAGGTCCCAGAGGAGGGGCCGGGG
GCCCCAGCCAGAGTGATGACCAAGAAGGAGGAGCCCCCACCGAGCCGAGTCCCACGGGCC
GTGCGCGTGGCCAGTCTGCTGAGCGAGGGAGAGGAACTGTCTGGGGACGACCAAGGGGAT
GAGGAAGAGGATGACCACGCCTATGAGGGCGTCCCCAATGGCGGATGGCATACCAGCAGC
CTGAGCTTGTCCTTGCCCAGCACAATAGCTGCGCCACACCCCATGGACGGGCCGCCTGGG
GGCTCCACCCCCGTCACACCAGTCATCAAGGCTGGCTGGCTGGACAAGAACCCACCGCAG
GGATCTTACATCTATCAGAAACGATGGGTGAGACTGGATACTGATCACCTGCGATACTTT
GACAGTAACAAGGACGCTTACTCTAAGCGCTTTATCTCTGTGGCCTGCATCTCCCACGTG
GCTGCCATCGGGGACCAGAAGTTTGAAGTGATCACAAACAACCGAACCTTTGCCTTCCGG
GCAGAGAGTGATGTGGAGCGGAAGGAGTGGATGCAGGCCCTGCAGCAGGCCATGGCTGAG
CAGCGTGCCCGGGCCCGGCTCTCTAGCGCTTATCTGCTGGGAGTTCCAGGCTCAGAGCAG
CCTGACCGCGCTGGCAGCCTGGAGCTTCGTGGCTTCAAGAATAAGCTGTACGTGGCCGTG
GTCGGGGACAAAGTGCAGCTCTACAAGAATCTAGAGGAGTACCACCTGGGCATTGGCATC
ACCTTCATCGACATGAGCGTGGGCAACGTGAAGGAAGTGGACCGGCGCAGCTTCGACCTC
ACCACGCCCTACCGCATCTTCAGCTTCTCTGCTGACTCAGAGCTAGAGAAGGAGCAGTGG
TTGGAGGCCATGCAGGGAGCCATCGCTGAGGCCCTGTCTACCTCGGAGGTGGCCGAGCGC
ATCTGGGCTGCAGCCCCCAACAGGTTCTGTGCTGACTGCGGGGCTCCTCAGCCTGACTGG
GCCTCCATCAACCTCTGTGTTGTTATCTGCAAGCGCTGTGCAGGGGAGCACCGTGGCCTG
GGCGCTGGCGTCTCCAAGGTGCGGAGCCTGAAGATGGACAGGAAGGTGTGGACAGAAACA
CTTATCGAGCTCTTCTTACAGCTGGGGAATGGCGCTGGGAACCGCTTCTGGGCAGCCAAC
GTGCCCCCCAGTGAGGCCCTGCAGCCCAGCAGCAGCCCCAGCACCCGGCGGTGCCACCTG
GAGGCCAAGTACCGTGAGGGCAAGTACCGCCGCTACCACCCGCTCTTTGGCAACCAGGAG
GAGCTGGACAAGGCCCTGTGTGCTGCAGTCACCACCACAGACCTGGCTGAGACCCAGGCG
CTCCTGGGCTGTGGGGCTGGGATCAACTGCTTCTCGGGGGACCCTGAGGCCCCCACGCCC
CTGGCTCTTGCAGAGCAGGCGGGGCAGACGCTGCAGATGGAATTCCTTCGGAACAACCGG
ACCACAGAGGTGCCTCGGCTGGACTCGATGAAGCCCCTGGAAAAGCACTACTCAGTTGTC
CTGCCGACCGTGAGCCACAGTGGCTTCCTCTACAAGACTGCCTCTGCCGGCAAGCTGCTA
CAGGACCGCCGGGCCCGGGAAGAGTTCAGCCGGCGCTGGTGTGTCCTTGGTGACGGGGTC
CTGAGCTACTTTGAGAATGAGCGGGCAGTGACCCCCAATGGAGAGATTCGGGCCAGCGAG
ATTGTGTGCCTGGCAGTGCCCCCTCCTGACACCCATGGCTTTGAGCACACCTTTGAGGTG
TACACGGAGGGAGAACGGCTGTACCTGTTTGGGCTGGAGAGTGCGGAGCAGGCTCATGAG
TGGGTCAAGTGTATTGCTAAGGCATTCGTGCCTCCCCTAGCCGAGGATCTGCTGGCCCGG
GATTTTGAGCGGCTGGGACGCCTACCCTACAAAGCTGGCCTGAGCCTACAGCGGGCCCAG
GAGGGCTGGTTCTCTCTCAGTGGCTCGGAGCTCCGTGCTGTCTTCCCGGAGGGGCCCTGC
GAAGAGCCGCTGCAACTACGGAAACTGCAGGAGCTTTCCATCCAGGGGGACAGTGAGAAC
CAGGTGCTGGTGCTGGTGGAGCGAAGGAGGACACTGTACATACAGGGCGAGCGGCGGCTG
GACTTCATGGGTTGGCTGGGGGCCATCCAGAAAGCAGCCGCCAGCATGGGGGACACGCTG
TCGGAGCAGCAGCTTGGGGACTCGGATATCCCGGTGATCGTGTACCGCTGTGTGGACTAC
ATCACGCAGTGCGGCCTGACCTCCGAGGGCATCTACCGCAAGTGTGGGCAGACATCGAAG
ACACAGCGGCTGCTGGAGAGCCTGCGGCAGGATGCGCGCTCTGTGCACCTCAAGGAGGGC
GAGCAGCACGTGGATGATGTTTCCTCGGCGCTCAAGCGCTTCCTGCGCGACCTGCCTGAT
GGGCTCTTCACTCGCGCCCAGCGCCTAACCTGGCTGGAGGCCTCAGAGATTGAGGACGAG
GAGGAGAAGGTCTCCAGGTACCGAGAGCTGCTGGTGCGGCTGCCCCCTGTCAACCGGGCC
ACAGTGAAGGCCCTTATCAGCCACCTGTACTGTGTTCAGTGCTTCTCAGACACGAACCAG
ATGAACGTGCACAACCTGGCAATTGTGTTTGGGCCCACGCTCTTCCAGACAGATGGGCAG
GACTACAAGGCTGGCCGTGTGGTGGAAGACCTCATTAACCACTATGTGGTGGTGTTTAGT
GTGGATGAGGAAGAGCTCAGGAAGCAGCGGGAGGAGATCACTGCCATTGTGAAGATGCGC
GTGGCTGGCACTGCCAGTGGGACCCAGCATGCCGGTGACTTCATCTGCACAGTGTATCTG
GAAGAGAAGAAGGCAGAGACTGAGCAGCATATCAAGGTCCCAGCATCCATGACTGCTGAG
GAGCTCACCCTGGAGATCCTGGATCGCCGGAACGTGGGCATCAGGGAGAAGGACTATTGG
ACCTGCTTTGAGGTCAACGAGAGGGAGGAGGCAGAGCGCCCCCTGCACTTTGCGGAGAAG
GTGCTGCCCATCCTGCACGGGCTGGGCACGGACAGCCACCTGGTGGTGAAGAAGCACCAG
GCCATGGAGGCCATGCTGCTGTACCTGGCCAGCCGTGTCGGTGACACCAAGCATGGCATG
ATGAAGTTCCGTGAGGACCGCAGCCTCCTGGGCCTGGGCCTGCCCTCAGGTGGCTTCCAC
GATCGCTACTTCATCCTCAACAGCAGCTGCTTGCGGCTCTACAAGGAGGTCCGGAGCCAG
AGGCCGTGGAGCGGGGCCCCTGAGACCAGTCACCGGCCTGAGAAGGAGTGGCCTATTAAG
AGTCTCAAAGTCTACCTGGGAGTGAAGAAGAAACTCAGGCCACCCACCTGCTGGGGCTTC
ACAGTGGTGCATGAGACAGAGAAACATGAGAAGCAGCAGTGGTACCTCTGCTGTGACACA
CAGATGGAGCTCCGGGAGTGGTTCGCTACCTTTCTGTTTGTGCAGCATGACGGCCTGGTG
TGGCCCTCAGAGCCCTCACGCGTGTCCCGGGCAGTGCCTGAGGTCCGGCTGGGTAGTGTG
TCACTGATCCCCCTTCGAGGTAGTGAAAATGAAATGCGCCGGAGTGTGGCTGCCTTCACC
GCGGACCCTCTGTCTCTTCTGCGCAACGTCTTG
Protein sequence predicted based on nucleotide sequence
MAEAGDAALSVAEWLRALHLEQYTGLFEQHGLVWATECQGLSDTRLMDMGMLLPGHRRRI LAGLLRAHTSPAPAPRPTPRPVPMKRHIFRSPPVPATPPEPLPTTTEDEGLPAAPPIPPR RSCLPPTCFTTPSTAAPDPVLPPLPAKRHLAELSVPPVPPRTGPPRLLVSLPTKEEESLL PSLSSPPQPQSEEPLSTLPQGPPQPPSPPPCPPEIPPKPVRLFPEFDDSDYDEVPEEGPG APARVMTKKEEPPPSRVPRAVRVASLLSEGEELSGDDQGDEEEDDHAYEGVPNGGWHTSS LSLSLPSTIAAPHPMDGPPGGSTPVTPVIKAGWLDKNPPQGSYIYQKRWVRLDTDHLRYF DSNKDAYSKRFISVACISHVAAIGDQKFEVITNNRTFAFRAESDVERKEWMQALQQAMAE QRARARLSSAYLLGVPGSEQPDRAGSLELRGFKNKLYVAVVGDKVQLYKNLEEYHLGIGI TFIDMSVGNVKEVDRRSFDLTTPYRIFSFSADSELEKEQWLEAMQGAIAEALSTSEVAER IWAAAPNRFCADCGAPQPDWASINLCVVICKRCAGEHRGLGAGVSKVRSLKMDRKVWTET LIELFLQLGNGAGNRFWAANVPPSEALQPSSSPSTRRCHLEAKYREGKYRRYHPLFGNQE ELDKALCAAVTTTDLAETQALLGCGAGINCFSGDPEAPTPLALAEQAGQTLQMEFLRNNR TTEVPRLDSMKPLEKHYSVVLPTVSHSGFLYKTASAGKLLQDRRAREEFSRRWCVLGDGV LSYFENERAVTPNGEIRASEIVCLAVPPPDTHGFEHTFEVYTEGERLYLFGLESAEQAHE WVKCIAKAFVPPLAEDLLARDFERLGRLPYKAGLSLQRAQEGWFSLSGSELRAVFPEGPC EEPLQLRKLQELSIQGDSENQVLVLVERRRTLYIQGERRLDFMGWLGAIQKAAASMGDTL SEQQLGDSDIPVIVYRCVDYITQCGLTSEGIYRKCGQTSKTQRLLESLRQDARSVHLKEG EQHVDDVSSALKRFLRDLPDGLFTRAQRLTWLEASEIEDEEEKVSRYRELLVRLPPVNRA TVKALISHLYCVQCFSDTNQMNVHNLAIVFGPTLFQTDGQDYKAGRVVEDLINHYVVVFS VDEEELRKQREEITAIVKMRVAGTASGTQHAGDFICTVYLEEKKAETEQHIKVPASMTAE ELTLEILDRRNVGIREKDYWTCFEVNEREEAERPLHFAEKVLPILHGLGTDSHLVVKKHQ AMEAMLLYLASRVGDTKHGMMKFREDRSLLGLGLPSGGFHDRYFILNSSCLRLYKEVRSQ RPWSGAPETSHRPEKEWPIKSLKVYLGVKKKLRPPTCWGFTVVHETEKHEKQQWYLCCDT QMELREWFATFLFVQHDGLVWPSEPSRVSRAVPEVRLGSVSLIPLRGSENEMRRSVAAFT ADPLSLLRNVL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
