Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860476
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4662nts
Open reading frame: 1 to 4662
ATGGAGCCCTCAGCCCTGAGGAAAGCTGGCTCGGAGCAGGAGGAGGGCTTTGAGGGGCTG
CCCAGAAGGGTCACTGACCTGGGGATGGTCTCCAATCTCCGGCGCAGCAACAGCAGCCTC
TTCAAGAGCTGGAGGCTACAGTGCCCCTTCGGCAACAATGACAAGCAAGAAAGCCTCAGT
TCGTGGATTCCTGAAAACATCAAGAAGAAAGAATGCGTGTATTTTGTGGAAAGTTCCAAA
CTGTCTGATGCTGGGAAGGTGGTGTGTCAGTGTGGCTACACGCATGAGCAGCACTTGGAG
GAGGCTACCAAGCCCCACACCTTCCAGGGCACACAGTGGGACCCAAAGAAACATGTCCAG
GAGATGCCAACCGATGCCTTTGGCGACATCGTCTTCACGGGCCTGAGCCAGAAGGTGAAA
AAGTACGTCCGAGTCTCCCAGGACACGCCCTCCAGCGTGATCTACCACCTCATGACCCAG
CACTGGGGGCTGGACGTCCCCAATCTCTTGATCTCGGTGACCGGGGGGGCCAAGAACTTC
AACATGAAGCCGCGGCTGAAGAGCATTTTCCGCAGAGGCCTGGTCAAGGTGGCTCAGACC
ACAGGGGCCTGGATCATCACAGGGGGGTCCCACACCGGCGTCATGAAGCAGGTAGGCGAG
GCGGTGCGGGACTTCAGCCTGAGCAGCAGCTACAAGGAAGGCGAGCTCATCACCATCGGA
GTCGCCACCTGGGGCACTGTCCACCGCCGCGAGGGCCTGATCCATCCCACGGGCAGCTTC
CCCGCCGAGTACATACTGGATGAGGATGGCCAAGGGAACCTGACCTGCCTAGACAGCAAC
CACTCTCACTTCATCCTCGTGGACGACGGGACCCACGGCCAGTACGGGGTGGAGATTCCT
CTGAGGACCAGGCTGGAGAAGTTCATATCGGAGCAGACCAAGGAAAGAGGAGGTGTGGCC
ATCAAGATCCCCATCGTGTGCGTGGTGCTGGAGGGCGGCCCGGGCACGTTGCACACCATC
GACAACGCCACCACCAACGGCACCCCCTGTGTGGTTGTGGAGGGCTCGGGCCGCGTGGCC
GACGTCATTGCCCAGGTGGCCAACCTGCCTGTCTCGGACATCACTATCTCCCTGATCCAG
CAGAAACTGAGCGTGTTCTTCCAGGAGATGTTTGAGACCTTCACGGAAAGCAGGATTGTC
GAGTGGACCAAAAAGATCCAAGATATCGTCCGGAGGCGGCAGCTGCTGACTGTCTTCCGG
GAAGGCAAGGATGGTCAGCAGGACGTGGATGTGGCCATCTTGCAGGCCTTGCTGAAAGCC
TCACGGAGCCAAGACCACTTTGGCCACGAGAACTGGGACCACCAGCTGAAACTGGCAGTG
GCATGGAATCGCGTGGACATTGCCCGCAGTGAGATCTTCATGGATGAGTGGCAGTGGAAG
CCTTCAGATCTGCACCCCACGATGACAGCTGCACTCATCTCCAACAAGCCTGAGTTTGTG
AAGCTCTTCCTGGAGAACGGGGTGCAGCTGAAGGAGTTTGTCACCTGGGACACCTTGCTC
TACCTGTACGAGAACCTGGACCCCTCCTGCCTGTTCCACAGCAAGCTGCAGAAGGTGCTG
GTGGAGGATCCCGAGCGCCCGGCTTGCGCGCCCGCGGCGCCCCGCCTGCAGATGCACCAC
GTGGCCCAGGTGCTGCGGGAGCTGCTGGGGGACTTCACGCAGCCGCTTTATCCCCGGCCC
CGGCACAACGACCGGCTGCGGCTCCTGCTGCCCGTTCCCCACGTCAAGCTCAACGTGCAG
GGAGTGAGCCTCCGGTCCCTCTACAAGCGTTCCTCAGGCCATGTGACCTTCACCATGGAC
CCCATCCGTGACCTTCTCATTTGGGCCATTGTCCAGAACCGTCGGGAGCTGGCAGGAATC
ATCTGGGCTCAGAGCCAGGACTGCATCGCAGCGGCCTTGGCCTGCAGCAAGATCCTGAAG
GAACTGTCCAAGGAGGAGGAGGACACGGACAGCTCGGAGGAGATGCTGGCGCTGGCGGAG
GAGTATGAGCACAGAGCCATCGGGGTCTTCACCGAGTGCTACCGGAAGGACGAAGAGAGA
GCCCAGAAACTGCTCACCCGCGTGTCCGAGGCCTGGGGGAAGACCACCTGCCTGCAGCTC
GCCCTGGAGGCCAAGGACATGAAGTTTGTGTCTCACGGGGGCATCCAGGCCTTCCTGACC
AAGGTGTGGTGGGGCCAGCTCTCCGTGGACAATGGGCTGTGGCGTGTGACCCTGTGCATG
CTGGCCTTCCCGCTGCTCCTCACCGGCCTCATCTCCTTCAGGGAGAAGAGGCTGCAGGAT
GTGGGCACCCCCGCGGCCCGCGCCCGTGCCTTCTTCACCGCACCCGTGGTGGTCTTCCAC
CTGAACATCCTCTCCTACTTCGCCTTCCTCTGCCTGTTCGCCTACGTGCTCATGGTGGAC
TTCCAGCCTGTGCCCTCCTGGTGCGAGTGTGCCATCTACCTCTGGCTCTTCTCCTTGGTG
TGCGAGGAGATGCGGCAGCTCTTCTATGACCCTGACGAGTGCGGGCTGATGAAGAAGGCA
GCCTTGTACTTCAGTGACTTCTGGAATAAGCTGGACGTCGGCGCAATCTTGCTCTTCGTG
GCAGGGCTGACCTGCAGGCTCATCCCGGCGACGCTGTACCCCGGGCGCGTCATCCTCTCT
CTGGACTTCATCCTGTTCTGCCTCCGGCTCATGCACATTTTTACCATCAGTAAGACGCTG
GGGCCCAAGATCATCATTGTGAAGCGGATGATGAAGGACGTCTTCTTCTTCCTCTTCCTG
CTGGCTGTGTGGGTGGTGTCCTTCGGGGTGGCCAAGCAGGCCATCCTCATCCACAACGAG
CGCCGGGTGGACTGGCTGTTCCGAGGGGCCGTCTACCACTCCTACCTCACCATCTTCGGG
CAGATCCCGGGCTACATCGACGGTGTGAACTTCAACCCGGAGCACTGCAGCCCCAATGGC
ACCGACCCCTACAAGCCTAAGTGCCCCGAGAGCGACGCGACGCAGCAGAGGCCGGCCTTC
CCTGAGTGGCTGACGGTCCTCCTACTCTGCCTCTACCTGCTCTTCACCAACATCCTGCTG
CTCAACCTCCTCATCGCCATGTTCAACTACACCTTCCAGCAGGTGCAGGAGCACACGGAC
CAGATTTGGAAGTTCCAGCGCCATGACCTGATCGAGGAGTACCACGGCCGCCCCGCCGCG
CCGCCCCCCTTCATCCTCCTCAGCCACCTGCAGCTCTTCATCAAGAGGGTGGTCCTGAAG
ACTCCGGCCAAGAGGCACAAGCAGCTCAAGAACAAGCTGGAGAAGAACGAGGAGGCGGCC
CTGCTATCCTGGGAGATCTACCTGAAGGAGAACTACCTCCAGAACCGACAGTTCCAGCAA
AAGCAGCGGCCCGAGCAGAAGATCGAGGACATCAGCAATAAGGCAGGGCTGGAGCTTTGG
GGGTCAAGGACAAGCTCTGTCTGCAAATCTAGACAGTTCCTGCATCTCACGGTGGTGGAA
TCACGCGGGTCCGAGAAGGCTCCAGTAACCACCCTCGCATGTTTGCAAGGCTGTCTGGGA
AAACATGGCAGGGTTGACGCCATGGTGGACCTGCTGGACCTGGACCCACTGAAGAGGTCG
GGCTCCATGGAGCAGAGGTTGGCCTCCCTGGAGGAGCAGGTGGCCCAGACAGCCCGAGCC
CTGCACTGGATCGTGAGGACGCTGCGGGCCAGCGGCTTCAGCTCGGAGGCGGACGTCCCC
ACTCTGGCCTCCCAGAAGGCCGCGGAGGAGCCGGATGCTGAGCCGGGAGGCAGGAAGAAG
ACGGAGGAGCCGGGCGACAGCTACCACGTGAATGCCCGGCACCTCCTCTACCCCAACTGC
CCTGTCACGCGCTTCCCCGTGCCCAACGAGAAGGTGCCCTGGGAGACGGAGTTCCTGATC
TATGACCCACCCTTTTACACGGCAGAGAGGAAGGACGCGGCCGCCATGGACCCCATGGGA
GACACCCTGGAGCCACTGTCCACGATCCAGTACAACGTGGTGGATGGCCTGAGGGACCGC
CGGAGCTTCCACGGGCCGTACACAGTGCAGGCCGGGTTGCCCCTGAACCCCATGGGCCGC
ACAGGACTGCGTGGGCGCGGGAGCCTCAGCTGCTTCGGACCCAACCACACGCTGTACCCC
ATGGTCACGCGGTGGAGGCGGAACGAGGATGGAGCCATCTGCAGGAAGAGCATAAAGAAG
ATGCTGGAAGTGCTGGTGGTGAAGCTCCCTCTCTCCGAGCACTGGGCCCTGCCTGGGGGC
TCCCGGGAGCCAGGGGAGATGCTACCTCGGAAGCTGAAGCGGATCCTCCGGCAGGAGCAC
TGGCCGTCTTTTGAAAACTTGCTGAAGTGCGGCATGGAGGTGTACAAAGGCTACATGGAT
GACCCGAGGAACACGGACAATGCCTGGATCGAGACGGTGGCCGTCAGCGTCCACTTCCAG
GACCAGAATGACGTGGAGCTGAACAGGCTGAACTCTAACCTGCACGCCTGCGACTCGGGG
GCCTCCATCCGATGGCAGGTGGTGGACAGGCGCATCCCACTCTATGCGAACCACAAGACC
CTCCTCCAGAAGGCAGCCGCTGAGTTCGGGGCTCACTACTTG
Protein sequence predicted based on nucleotide sequence
MEPSALRKAGSEQEEGFEGLPRRVTDLGMVSNLRRSNSSLFKSWRLQCPFGNNDKQESLS SWIPENIKKKECVYFVESSKLSDAGKVVCQCGYTHEQHLEEATKPHTFQGTQWDPKKHVQ EMPTDAFGDIVFTGLSQKVKKYVRVSQDTPSSVIYHLMTQHWGLDVPNLLISVTGGAKNF NMKPRLKSIFRRGLVKVAQTTGAWIITGGSHTGVMKQVGEAVRDFSLSSSYKEGELITIG VATWGTVHRREGLIHPTGSFPAEYILDEDGQGNLTCLDSNHSHFILVDDGTHGQYGVEIP LRTRLEKFISEQTKERGGVAIKIPIVCVVLEGGPGTLHTIDNATTNGTPCVVVEGSGRVA DVIAQVANLPVSDITISLIQQKLSVFFQEMFETFTESRIVEWTKKIQDIVRRRQLLTVFR EGKDGQQDVDVAILQALLKASRSQDHFGHENWDHQLKLAVAWNRVDIARSEIFMDEWQWK PSDLHPTMTAALISNKPEFVKLFLENGVQLKEFVTWDTLLYLYENLDPSCLFHSKLQKVL VEDPERPACAPAAPRLQMHHVAQVLRELLGDFTQPLYPRPRHNDRLRLLLPVPHVKLNVQ GVSLRSLYKRSSGHVTFTMDPIRDLLIWAIVQNRRELAGIIWAQSQDCIAAALACSKILK ELSKEEEDTDSSEEMLALAEEYEHRAIGVFTECYRKDEERAQKLLTRVSEAWGKTTCLQL ALEAKDMKFVSHGGIQAFLTKVWWGQLSVDNGLWRVTLCMLAFPLLLTGLISFREKRLQD VGTPAARARAFFTAPVVVFHLNILSYFAFLCLFAYVLMVDFQPVPSWCECAIYLWLFSLV CEEMRQLFYDPDECGLMKKAALYFSDFWNKLDVGAILLFVAGLTCRLIPATLYPGRVILS LDFILFCLRLMHIFTISKTLGPKIIIVKRMMKDVFFFLFLLAVWVVSFGVAKQAILIHNE RRVDWLFRGAVYHSYLTIFGQIPGYIDGVNFNPEHCSPNGTDPYKPKCPESDATQQRPAF PEWLTVLLLCLYLLFTNILLLNLLIAMFNYTFQQVQEHTDQIWKFQRHDLIEEYHGRPAA PPPFILLSHLQLFIKRVVLKTPAKRHKQLKNKLEKNEEAALLSWEIYLKENYLQNRQFQQ KQRPEQKIEDISNKAGLELWGSRTSSVCKSRQFLHLTVVESRGSEKAPVTTLACLQGCLG KHGRVDAMVDLLDLDPLKRSGSMEQRLASLEEQVAQTARALHWIVRTLRASGFSSEADVP TLASQKAAEEPDAEPGGRKKTEEPGDSYHVNARHLLYPNCPVTRFPVPNEKVPWETEFLI YDPPFYTAERKDAAAMDPMGDTLEPLSTIQYNVVDGLRDRRSFHGPYTVQAGLPLNPMGR TGLRGRGSLSCFGPNHTLYPMVTRWRRNEDGAICRKSIKKMLEVLVVKLPLSEHWALPGG SREPGEMLPRKLKRILRQEHWPSFENLLKCGMEVYKGYMDDPRNTDNAWIETVAVSVHFQ DQNDVELNRLNSNLHACDSGASIRWQVVDRRIPLYANHKTLLQKAAAEFGAHYL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
