Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00860592
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4548nts
Open reading frame: 1 to 4548
ATGGAGCCCCGCAGCATGGAGTACTTCTGCGCCCAGGTGCAGCAGAAGGACGTCGGCGGC
CGGCTGCAGGTCGGCCAGGAGCTCCTGCTCTACCTTGGCGCCCCCGGCGCCATCTCGGAC
CTGGAGGAGGACCTGGGCCGCCTAGGCAAGACAGTCGACGCGCTCACCGGCTGGGTGGGT
TCGAGCAACTACCGGGTATCATTAATGGGATTGGAAATTTTAAGTGCCTTTGTGGACAGA
TTATCAACACGCTTTAAATCCTATGTAGCAATGGTTATTGTAGCTTTAATAGACAGAATG
GGAGATGCCAAAGACAAGGTTCGAGATGAAGCTCAGACTCTGATATTGAAGTTAATGGAT
CAAGTAGCACCACCTATGTACATTTGGGAGCAGTTGGCTTCTGGTTTTAAACACAAGAAT
TTTCGATCTCGAGAAGGCGTGTGTCTGTGTCTTATTGAAACCTTAAACATTTTTGGGGCT
CAGCCACTAGTCATCAGCAAATTGATACCACATTTGTGTATCCTGTTTGGAGACTCCAAC
AGTCAGGTGAGAGATGCTGCAATATTGGCTATAGTGGAGATTTATAGACATGTGGGAGAA
AAAGTGAGGATGGATCTTTATAAGAGAGGAATTCCCCCTGCTAGATTAGAAATGATATTT
GCCAAATTTGATGAAGTGCAAAGTTCAGGCGGTATGATTTTGAGTGTCTGCAAAGATAAA
AGCTTCGATGATGAAGAATCAGTGGATGGAAATAGGCCATCATCAGCTGCATCAGCCTTC
AAGGTTCCTGCACCTAAAACATCCGGAAATCCTGCCAACAGTGCAAGGAAGCCTGGTTCA
GCAGGTGGCCCTAAGGTTGGAGCAGGTGCTTCTAAGGAAGGAGGTGCTGGAGCAGTTGAT
GAAGATGATTTTATAAAAGCTTTTACAGATGTCCCTTCTATTCAGATTTATTCTAGTCGA
GAACTCGAAGAAACATTAAATAAAATCAGGGAAATTTTGTCAGATGATAAACATGACTGG
GATCAGCGTGCCAATGCACTGAAGAAAATTCGATCACTGCTTGTTGCTGGAGCTGCACAG
TATGATTGCTTTTTTCAACATTTACGATTGTTGGATGGAGCACTTAAACTTTCAGCTAAG
GATCTTAGATCCCAGGTGGTTAGAGAAGCTTGTATTACTGTAGCCCACCTTTCAACAGTT
TTGGGAAACAAGTTTGATCATGGCGCTGAAGCCATTGTACCTACACTTTTTAATCTCGTC
CCCAATAGTGCAAAAGTCATGGCAACTTCTGGATGTGCAGCAATCAGATTTATCATTCGG
CATACTCATGTACCCAGACTTATACCTTTAATAACAAGCAATTGCACATCAAAATCAGTT
CCCGTGAGGAGACGTTCATTTGAATTTTTAGATTTATTGTTGCAAGAGTGGCAGACTCAT
TCATTGGAAAGACATGCAGCCGTCTTGGTTGAAACTATTAAAAAGGGAATTCATGATGCT
GACGCTGAGGCCAGAGTGGAGGCAAGAAAGACATACATGGGTCTTAGAAACCACTTTCCT
GGTGAAGCTGAAACATTATATAATTCCCTTGAGCCATCTTATCAGAAGAGTCTTCAAACT
TACTTAAAGAGTTCTGGCAGTGTAGCATCTCTTCCACAATCAGACAGGTCCTCATCCAGC
TCACAGGAAAGTCTCAATCGCCCTTTTTCTTCCAAATGGTCTACAGCAAATCCATCAACT
GTGGCTGGAAGAGTATCAGCAGGCAGCAGCAAAGCCAGTTCCCTTCCAGGAAGCCTGCAG
CGTTCACGAAGTGACATTGATGTGAATGCTGCTGCAGGTGCCAAGGCACATCATGCTGCT
GGACAGTCTGTGCGAAGCGGGCGCTTAGGTGCAGGTGCCCTGAATGCAGGTTCCTATGCG
TCACTAGAGGATACTTCTGACAAGCTGGATGGAACAGCATCTGAAGATGGCCGGGTGAGA
GCAAAACTTTCAGCACCACTTGCTGGCATGGGAAATGCCAAGGCAGATTCTAGAGGAAGA
AGTCGAACAAAAATGGTGTCTCAATCACAGCCTGGTAGCCGGTCTGGGTCTCCAGGAAGA
GTTCTGACCACAACAGCCCTGTCCACTGTGAGCTCTGGTGTTCAAAGAGTCCTGGTCAAT
TCAGCCTCAGCACAAAAAAGAAGCAAGATACCACGGAGCCAGGGCTGTAGCAGAGAGGCT
AGTCCATCTAGGCTTTCAGTGGCCCGAAGCAGTCGTATTCCTCGACCAAGTGTGAGTCAA
GGATGCAGCCGGGAAGCTAGTCGGGAGAGCAGCAGAGACACAAGTCCTGTTCGCTCTTTT
CAGCCCCTCGGTCCAGGTTATGGGATCAGCCAATCAAGTCGACTGTCGTCTTCTGTTAGT
GCCATGCGAGTCCTGAACACAGGTTCTGATGTGGAGGAGGCGGTGGCAGATGCCTTGCTC
TTAGGAGACATACGGACTAAGAAAAAACCAGCTCGAAGAAGATATGAATCATATGGAATG
CATTCAGATGATGACGCCAACAGCGATGCATCTAGTGCTTGTTCAGAACGCTCCTATAGT
TCTCGAAATGGTAGTATTCCTACATATATGAGGCAGACGGAAGATGTGGCAGAAGTCCTC
AATAGATGTGCTAGTTCCAATTGGTCAGAAAGGAAAGAAGGCCTCCTAGGTCTGCAGAAC
TTATTAAAAAATCAGAGAACACTAAGTCGAGTTGAACTGAAAAGATTATGTGAAATTTTC
ACAAGAATGTTTGCTGACCCTCATGGCAAGAGAGTATTCAGCATGTTTTTGGAGACTCTA
GTGGATTTCATACAAGTCCACAAAGATGATCTTCAAGATTGGTTGTTTGTACTGCTGACA
CAACTACTAAAAAAAATGGGTGCTGATTTGCTTGGATCTGTTCAGGCAAAAGTTCAGAAA
GCCCTTGATGTTACAAGAGAGTCTTTTCCAAATGATCTTCAGTTCAATATTCTAATGAGA
TTTACAGTTGATCAGACCCAGACACCAAGCTTAAAGGTGAAGGTTGCTATCCTTAAATAC
ATAGAAACTCTGGCCAAACAGATGGATCCAGGAGATTTTATAAATTCCAGTGAAACTCGC
CTAGCAGTGTCTCGGGTCATCACTTGGACAACAGAACCCAAAAGTTCTGATGTTCGGAAG
GCAGCACAGTCAGTGCTGATTTCATTATTTGAACTCAATACCCCAGAGTTTACAATGTTA
TTAGGAGCTTTACCAAAAACTTTTCAGGATGGTGCTACCAAGCTTCTTCATAATCACCTT
CGAAACACTGGCAATGGAACCCAGAGTTCCATGGGGAGTCCTTTGACAAGACCAACACCA
CGATCACCAGCTAACTGGTCCAGTCCTCTTACTTCTCCTACCAATACATCACAGAATACT
TTATCTCCAAGTGCATTTGATTATGACACAGAAAATATGAACTCTGAAGATATTTATAGC
TCTCTTAGAGGTGTCACTGAAGCAATCCAGAATTTCAGCTTCCGTAGCCAAGAAGATATG
AATGAGCCATTGAAAAGGGATTCTAAAAAAGATGATGGCGATTCAATGTGTGGTGGTCCT
GGGATGTCTGACCCAAGAGCAGGAGGTGATGCTACTGACTCAAGTCAAACAGCTCTTGAT
AATAAAGCTTCATTGCTCCATTCAATGCCTACTCACTCCTCTCCACGCTCTCGAGACTAT
AATCCATATAACTATTCAGATAGCATCAGTCCCTTCAACAAGTCTGCCCTCAAGGAAGCC
ATGTTTGATGATGATGCTGACCAGTTTCCTGACGATCTTTCCCTAGATCATTCTGACCTA
GTTGCAGAGTTGTTGAAGGAGCTGTCTAACCATAATGAGCGTGTAGAAGAAAGAAAAATT
GCCCTCTATGAACTTATGAAACTGACACAGGAAGAATCTTTTAGTGTTTGGGATGAACAC
TTCAAAACAATATTGCTTTTATTGCTTGAAACGCTTGGAGATAAAGAGCCTACAATCAGG
GCTTTGGCATTAAAGGTTTTAAGAGAAATCCTAAGGCATCAACCAGCAAGATTTAAAAAC
TATGCAGAATTGACTGTCATGAAAACATTGGAAGCACATAAAGATCCTCATAAGGAGGTG
GTGAGATCTGCTGAGGAAGCGGCATCAGTGTTGGCCACTTCAATTAGTCCAGAGCAGTGC
ATCAAAGTGCTTTGTCCTATCATTCAAACTGCAGACTACCCAATTAATCTGGCTGCAATC
AAAATGCAAACAAAAGTGATAGAGAGAGTGTCCAAGGAAACCCTAAACCTGCTTTTGCCA
GAGATTATGCCAGGTCTAATACAGGGTTATGATAATTCAGAGAGCAGTGTTCGGAAAGCT
TGTGTCTTCTGCCTGGTGGCTGTTCATGCGGTAATTGGTGATGAACTAAAACCACATCTC
AGTCAACTTACTGGCAGTAAAATGAAGCTACTGAATCTTTACATCAAACGTGCACAAACA
GGTTCTGGAGGAGCTGATCCCACTACTGATGTTTCTGGACAAAGTTTG
Protein sequence predicted based on nucleotide sequence
MEPRSMEYFCAQVQQKDVGGRLQVGQELLLYLGAPGAISDLEEDLGRLGKTVDALTGWVG SSNYRVSLMGLEILSAFVDRLSTRFKSYVAMVIVALIDRMGDAKDKVRDEAQTLILKLMD QVAPPMYIWEQLASGFKHKNFRSREGVCLCLIETLNIFGAQPLVISKLIPHLCILFGDSN SQVRDAAILAIVEIYRHVGEKVRMDLYKRGIPPARLEMIFAKFDEVQSSGGMILSVCKDK SFDDEESVDGNRPSSAASAFKVPAPKTSGNPANSARKPGSAGGPKVGAGASKEGGAGAVD EDDFIKAFTDVPSIQIYSSRELEETLNKIREILSDDKHDWDQRANALKKIRSLLVAGAAQ YDCFFQHLRLLDGALKLSAKDLRSQVVREACITVAHLSTVLGNKFDHGAEAIVPTLFNLV PNSAKVMATSGCAAIRFIIRHTHVPRLIPLITSNCTSKSVPVRRRSFEFLDLLLQEWQTH SLERHAAVLVETIKKGIHDADAEARVEARKTYMGLRNHFPGEAETLYNSLEPSYQKSLQT YLKSSGSVASLPQSDRSSSSSQESLNRPFSSKWSTANPSTVAGRVSAGSSKASSLPGSLQ RSRSDIDVNAAAGAKAHHAAGQSVRSGRLGAGALNAGSYASLEDTSDKLDGTASEDGRVR AKLSAPLAGMGNAKADSRGRSRTKMVSQSQPGSRSGSPGRVLTTTALSTVSSGVQRVLVN SASAQKRSKIPRSQGCSREASPSRLSVARSSRIPRPSVSQGCSREASRESSRDTSPVRSF QPLGPGYGISQSSRLSSSVSAMRVLNTGSDVEEAVADALLLGDIRTKKKPARRRYESYGM HSDDDANSDASSACSERSYSSRNGSIPTYMRQTEDVAEVLNRCASSNWSERKEGLLGLQN LLKNQRTLSRVELKRLCEIFTRMFADPHGKRVFSMFLETLVDFIQVHKDDLQDWLFVLLT QLLKKMGADLLGSVQAKVQKALDVTRESFPNDLQFNILMRFTVDQTQTPSLKVKVAILKY IETLAKQMDPGDFINSSETRLAVSRVITWTTEPKSSDVRKAAQSVLISLFELNTPEFTML LGALPKTFQDGATKLLHNHLRNTGNGTQSSMGSPLTRPTPRSPANWSSPLTSPTNTSQNT LSPSAFDYDTENMNSEDIYSSLRGVTEAIQNFSFRSQEDMNEPLKRDSKKDDGDSMCGGP GMSDPRAGGDATDSSQTALDNKASLLHSMPTHSSPRSRDYNPYNYSDSISPFNKSALKEA MFDDDADQFPDDLSLDHSDLVAELLKELSNHNERVEERKIALYELMKLTQEESFSVWDEH FKTILLLLLETLGDKEPTIRALALKVLREILRHQPARFKNYAELTVMKTLEAHKDPHKEV VRSAEEAASVLATSISPEQCIKVLCPIIQTADYPINLAAIKMQTKVIERVSKETLNLLLP EIMPGLIQGYDNSESSVRKACVFCLVAVHAVIGDELKPHLSQLTGSKMKLLNLYIKRAQT GSGGADPTTDVSGQSL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
