Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00864171
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 5250nts
Open reading frame: 1 to 5250
ATGCACTTGAAGCACCTGAGGACCCTGCTGAGCCCTCAGGATGGAGCTGCAAAGGTGACC
TGCATGGCTTGGTCCCAGAACAATGCCAAATTTGCTGTCTGCACAGTGGACCGAGTGGTC
TTGCTGTATGATGAACATGGAGAACGGAGAGATAAATTCTCCACCAAACCAGCTGACATG
AAGTATGGCAGGAAGAGCTATATGGTGAAGGGCATGGCTTTTTCTCCTGATTCCACTAAA
ATTGCCATAGGACAGACTGACAACATCATCTATGTCTACAAGATTGGAGAAGATTGGGGT
GACAAGAAAGTCATCTGCAACAAGTTCATCCAGACGAGTGCTGTCACTTGTCTGCAATGG
CCGGCAGAATACATCATTGTCTTTGGACTGGCTGAAGGGAAGGTTCGTTTAGCAAACACC
AAAACTAATAAATCATCTACCATCTATGGGACAGAGTCTTACGTGGTGTCCCTGACAACA
AATTGCTCTGGGAAAGGAATTCTCTCTGGTCATGCAGATGGTACCATCGTTAGGTATTTC
TTTGATGATGAAGGCTCTGGAGAGTCACAGGGGAAGTTGGTTAACCACCCGTGTCCACCC
TATGCCTTGGCATGGGCAACCAATAGCATCGTGGCTGCAGGCTGTGATCGGAAAATTGTA
GCCTATGGAAAAGAAGGTCACATGCTACAAACTTTTGATTATAGCCGTGACCCTCAGGAG
CGGGAGTTCACCACAGCTGTATCAAGTCCTGGGGGCCAGTCTGTTGTGCTAGGAAGTTAT
GACAGGCTTCGGGTGTTCAACTGGATCCCTCGAAGAAGCATCTGGGAAGAGGCAAAGCCC
AAGGAGATTACCAATTTATACACCATCACTGCCTTGGCCTGGAAGCGGGATGGCTCACGG
CTCTGTGTGGGCACACTATGTGGTGGGGTGGAACAGTTTGACTGCTGCCTCCGAAGGAGT
ATTTACAAGAACAAGTTTGAGTTGACGTATGTGGGACCTAGCCAGGTGATTGTGAAGAAC
CTGTCATCAGGAACCCGAGTGGTGCTCAAGTCACACTATGGCTATGAGGTGGAAGAGGTG
AAAATCCTAGGAAAGGAACGTTACTTGGTGGCTCACACATCAGAAACACTGCTGCTGGGG
GACCTGAACACTAATCGGCTTAGTGAGATAGCCTGGCAAGGATCTGGTGGCAATGAGAAG
TATTTCTTTGAAAATGAGAATGTATGCATGATCTTCAATGCCGGAGAGCTAACCCTGGTG
GAATATGGGAATAATGACACCCTGGGTTCTGTACGCACTGAATTCATGAACCCCCACCTC
ATCAGTGTTCGTATTAATGAGAGGTGTCAGCGAGGAACAGAAGATAATAAGAAATTGGCT
TATCTTATTGATATTAAGACTATTGCTATAGTGGATCTGATTGGTGGCTACAACATTGGC
ACCGTCAGCCATGAGAGCCGTGTGGATTGGCTGGAACTTAATGAGACTGGACACAAGCTC
CTCTTCAGGGACCGGAAACTTCGTTTGCATCTGTATGATATTGAAAGCTGCTCTAAGACA
ATGATCCTCAACTTCTGCTCCTATATGCAGTGGGTCCCAGGAAGTGACGTGCTGGTAGCT
CAGAACCGAAACAGTCTGTGTGTATGGTACAACATTGAGGCACCTGAGAGAGTCACCATG
TTCACTATTAGGGGTGATGTTATAGGTCTGGAGCGGGGCGGGGGAAAGACCGAGGTGATG
GTGATGGAAGGTGTGACTACTGTTGCCTACACATTGGATGAGGGCCTCATCGAGTTTGGA
ACAGCCATTGATGATGGCAACTACATCCGGGCAACAGCCTTCTTAGAGACTCTGGAAATG
ACCCCAGAAACAGAGGCAATGTGGAAAACCTTGAGTAAACTGGCACTAGAGGCAAGGCAA
CTACACATTGCGGAGAGGTGCTTTTCTGCTTTGGGCCAAGTAGCAAAAGCTCGATTCCTG
CATGAGACCAATGAGATTGCAGATCAAGTATCCCGGGAATATGGCGGAGAAGGAACAGAC
TTTTATCAGGTCCGAGCACGTCTAGCCATGCTGGAAAAGAACTACAAACTGGCTGAAATG
ATCTTTTTGGAACAGAATGCTGTGGAGGAGGCCATGGGCATGTACCAGGAGCTACACCGT
TGGGATGAGTGTATCGCTGTGGCTGAAGCCAAGGGGCACCCAGCCCTGGAGAAGCTACGT
CGTAGTTACTACCAGTGGCTGATGGACACACAGCAAGAGGAGCGAGCAGGTGAACTACAG
GAGAGCCAAGGGGATGGGCTAGCAGCCATCAGCCTCTACCTCAAAGCTGGGCTCCCTGCC
AAAGCTGCTCGGCTGGTGCTGACCCGAGAGGAACTGCTAGCCAACACAGAGCTGGTAGAA
CACATCACTGCAGCCCTTATCAAGGGGGAACTCTACGAAAGGGCAGGTGATCTCTTTGAG
AAGATTCACAATCCACAGAAGGCCCTGGAGTGCTACCGTAAAGGCAACGCATTCATGAAA
GCGGTAGAGCTGGCTCGATTGGCCTTCCCAGTGGAGGTGGTGAAACTAGAGGAGGCATGG
GGGGACCACCTGGTGCAGCAGAAGCAGCTTGATGCAGCCATTAATCACTACATCGAAGCC
AGGTGCTCCATTAAGGCAATTGAGGCCGCCCTGGGTGCCCGCCAGTGGAAGAAGGCAATT
TATATATTAGATCTACAGGACCGGAACACTGCATCCAAATACTATCCTCTCGTGGCCCAA
CACTATGCATCCCTGCAGGAGTATGAGATTGCTGAGGAGCTCTATACTAAGGGAGATCGG
ACAAAAGATGCCATAGACATGTACACCCAGGCTGGTCGTTGGGAACAAGCCCACAAGCTG
GCGATGAAATGCATGAGACCAGAAGATGTGTCAGTGCTATACATCACTCAGGCCCAGGAA
ATGGAGAAGCAGGGCAAGTACCGTGAGGCTGAAAGGCTATATGTGACAGTACAAGAGCCT
GATCTTGCCATCACCATGTACAAAAAGCACAAGTTGTATGATGACATGATCCGCCTGGTA
GGGAAGCACCATCCAGATCTCCTCAGTGATACACACCTACATCTGGGCAAGGAGCTGGAG
GCTGAAGGCCGACTACAGGAGGCTGAGTACCACTACCTCGAGGCCCAGGAATGGAAGGCA
ACAGTGAACATGTACCGGGCCAGTGGGCTTTGGGAAGAGGCCTACAGGGTGGCCAGAACT
CAAGGAGGGGCTAATGCCCACAAACACGTGGCCTATCTGTGGGCAAAGAGCCTGGGAGGA
GAGGCTGCAGTTAGACTGCTTAATAAGCTGGGACTCCTGGAAGCTGCTGTTGACCACGCT
GCAGACAATTGCTCCTTTGAATTTGCGTTTGAACTCTCTCGGCTGGCCCTCAAGCACAAA
ACCCCCGAGGTTCATCTCAAATATGCTATGTTCCTGGAGGATGAGGGTAAATTCGAAGAG
GCTGAAGCTGAATTCATCAGAGCTGGTAAACCCAAGGAGGCAGTCCTCATGTTTGTCCAT
AACCAGGATTGGGAGGCAGCTCAGCGTGTGGCTGAGGCTCACGACCCTGACAGTGTCGCC
GAGGTGCTTGTGGGACAGGCCCGGGGGGCCTTGGAGGAGAAGGACTTTCAGAAAGCAGAA
GGGCTGCTGCTCCGGGCCCAGAGACCAGGCCTGGCCCTCAATTATTATAAGGAGGCTGGA
TTATGGAGTGACGCTCTGCGCATCTGCAAGGACTATGTGCCCAGCCAGCTGGAGGCTCTG
CAGGAAGAATATGAGCGGGAAGCTACTAAGAAGGGGGCCAGGGGTGTGGAGGGATTTGTG
GAACAAGCTCGACACTGGGAGCAGGCTGGAGAGTACAGCCGTGCCGTGGACTGCTACCTC
AAAGTGCGAGACTCTGGAAACAGCGGCCTGGCGGAGAAGTGCTGGATGAAGGCAGCTGAA
CTCTCCATCAAGTTTCTGCCTCCCCAACGTAATATGGAAGTCGTTCTGGCTGTAGGACCC
CAGCTGATTGGAATTGGAAAGCACAGTGCAGCTGCAGAGCTCTATCTGAATCTGGACCTT
GTCAAGGAAGCAATCGATGCTTTCATCGAGGGTGAGGAGTGGAACAAGGCGAAGCGTGTA
GCTAAGGAGTTAGATCCCAGGTATGAAGACTATGTGGACCAGCATTATAAAGAGTTCCTC
AAGAATCAGGGCAAAGTGGACTCGCTGGTGGGTGTGGATGTGATAGCTGCTTTGGACCTG
TATGTGGAGCAGGGCCAGTGGGACAAGTGCATTGAAACAGCTACCAAGCAGAACTACAAG
ATTCTGCACAAGTATGTGGCTTTGTATGCAACTCACTTGATCCGGGAGGGTAGCTCTGCC
CAGGCATTGGCCCTGTATGTACAGCACGGAGCCCCTGCTAACCCACAGAACTTCAATATC
TACAAAAGGATCTTCACTGACATGGTGAGCTCTCCTGGAACCAACTGTGCCGAGGCCTAT
CATAGCTGGGCTGATCTTCGAGATGTCCTCTTCAACCTGTGTGAAAACCTGGTGAAGTCC
AGTGAGGCAAACTCTCCAGCCCATGAGGAGTTCAAGACGATGCTGCTGATCGCTCATTAC
TATGCCACGCGCTCTGCAGCCCAGAGTGTCAAACAGCTGGAAACCGTGGCTGCCAGGCTT
TCTGTTTCACTCTTGCGTCACACCCAGCTACTACCTGTAGACAAAGCCTTCTATGAAGCA
GGCATTGCTGCCAAGGCAGTTGGCTGGGATAACATGGCATTCATCTTCCTCAATCGCTTT
TTGGACCTGACCGATGCAATCGAGGAAGGGACTCTAGATGGCCTTGACCACTCTGATTTT
CAGGATACAGACATTCCCTTTGAGGTGCCACTCCCAGCTAAGCAGCATGTACCGGAGGCT
GAGAGAGAAGAGGTTCGAGACTGGGTGCTTACAGTCTCCATGGACCAGCGGCTGGAGCAG
GTTCTGCCTCGGGATGAGCGTGGCGCCTACGAGGCCTCCCTAGTGGCAGCGAGCACTGGT
GTTCGAGCCCTGCCCTGCCTTATTACAGGATACCCCATTCTGAGGAACAAAATTGAATTT
AAGCGGCCAGGGAAGGCTGCTAACAAGGACAACTGGAATAAATTCCTTATGGCCATCAAG
ACCTCCCACAGCCCAGTGTGCCAGGACGTGCTGAAATTCATCAGTCAGTGGTGTGGAGGG
CTCCCCAGCACCAGCTTTTCCTTTCAGTTG
Protein sequence predicted based on nucleotide sequence
MHLKHLRTLLSPQDGAAKVTCMAWSQNNAKFAVCTVDRVVLLYDEHGERRDKFSTKPADM KYGRKSYMVKGMAFSPDSTKIAIGQTDNIIYVYKIGEDWGDKKVICNKFIQTSAVTCLQW PAEYIIVFGLAEGKVRLANTKTNKSSTIYGTESYVVSLTTNCSGKGILSGHADGTIVRYF FDDEGSGESQGKLVNHPCPPYALAWATNSIVAAGCDRKIVAYGKEGHMLQTFDYSRDPQE REFTTAVSSPGGQSVVLGSYDRLRVFNWIPRRSIWEEAKPKEITNLYTITALAWKRDGSR LCVGTLCGGVEQFDCCLRRSIYKNKFELTYVGPSQVIVKNLSSGTRVVLKSHYGYEVEEV KILGKERYLVAHTSETLLLGDLNTNRLSEIAWQGSGGNEKYFFENENVCMIFNAGELTLV EYGNNDTLGSVRTEFMNPHLISVRINERCQRGTEDNKKLAYLIDIKTIAIVDLIGGYNIG TVSHESRVDWLELNETGHKLLFRDRKLRLHLYDIESCSKTMILNFCSYMQWVPGSDVLVA QNRNSLCVWYNIEAPERVTMFTIRGDVIGLERGGGKTEVMVMEGVTTVAYTLDEGLIEFG TAIDDGNYIRATAFLETLEMTPETEAMWKTLSKLALEARQLHIAERCFSALGQVAKARFL HETNEIADQVSREYGGEGTDFYQVRARLAMLEKNYKLAEMIFLEQNAVEEAMGMYQELHR WDECIAVAEAKGHPALEKLRRSYYQWLMDTQQEERAGELQESQGDGLAAISLYLKAGLPA KAARLVLTREELLANTELVEHITAALIKGELYERAGDLFEKIHNPQKALECYRKGNAFMK AVELARLAFPVEVVKLEEAWGDHLVQQKQLDAAINHYIEARCSIKAIEAALGARQWKKAI YILDLQDRNTASKYYPLVAQHYASLQEYEIAEELYTKGDRTKDAIDMYTQAGRWEQAHKL AMKCMRPEDVSVLYITQAQEMEKQGKYREAERLYVTVQEPDLAITMYKKHKLYDDMIRLV GKHHPDLLSDTHLHLGKELEAEGRLQEAEYHYLEAQEWKATVNMYRASGLWEEAYRVART QGGANAHKHVAYLWAKSLGGEAAVRLLNKLGLLEAAVDHAADNCSFEFAFELSRLALKHK TPEVHLKYAMFLEDEGKFEEAEAEFIRAGKPKEAVLMFVHNQDWEAAQRVAEAHDPDSVA EVLVGQARGALEEKDFQKAEGLLLRAQRPGLALNYYKEAGLWSDALRICKDYVPSQLEAL QEEYEREATKKGARGVEGFVEQARHWEQAGEYSRAVDCYLKVRDSGNSGLAEKCWMKAAE LSIKFLPPQRNMEVVLAVGPQLIGIGKHSAAAELYLNLDLVKEAIDAFIEGEEWNKAKRV AKELDPRYEDYVDQHYKEFLKNQGKVDSLVGVDVIAALDLYVEQGQWDKCIETATKQNYK ILHKYVALYATHLIREGSSAQALALYVQHGAPANPQNFNIYKRIFTDMVSSPGTNCAEAY HSWADLRDVLFNLCENLVKSSEANSPAHEEFKTMLLIAHYYATRSAAQSVKQLETVAARL SVSLLRHTQLLPVDKAFYEAGIAAKAVGWDNMAFIFLNRFLDLTDAIEEGTLDGLDHSDF QDTDIPFEVPLPAKQHVPEAEREEVRDWVLTVSMDQRLEQVLPRDERGAYEASLVAASTG VRALPCLITGYPILRNKIEFKRPGKAANKDNWNKFLMAIKTSHSPVCQDVLKFISQWCGG LPSTSFSFQL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
