Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00866175
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3855nts
Open reading frame: 1 to 3855
ATGAGCTCCGGAGACCCTGCACACCTCGGCCTCTGCCTCTGGCTGTGGCTGGGCGCCACC
CTGGGAAGAGAGCAAGTTCAAGCAAGCGGTCTCCTGAGGCTGGCTGTGCTGCCTGAGGAC
CGGCTGCAGATGAAGTGGAGAGAGTCGGAGGGGAGCGGCCTCGGCTACCTGGTGCAGGTG
AAGCCCATGGCAGGGGACTCGGAACAGGAGGTGATACTGACCACCAAGACCCCTAAGGCC
ACAGTGGGGGGCCTGAGCCCCTCCAAGGGCTACACCTTGCAGATCTTCGAGCTCACTGGC
TCTGGGCGCTTCCTGCTAGCTCGGAGGGAGTTTGTGATTGAGGATCTGAAGAGTAGCTCC
CTGGACAGGAGCAGCCAGAGGCCCCTCGGCTCTGGAGCCCCGGAGCCCACCCCCTCCCAC
ACGGGGAGCCCAGACCCTGAGCAGGCTTCTGAGCCCCAAGTTGCCTTCACACCAAGCCAG
GATCCGCGCACTCCTGCCGGCCCCCAGTTCCGCTGCCTGCCCCCCGTGCCTGCTGACATG
GTCTTCCTGGTGGACGGGTCCTGGAGCATTGGCCACAGTCACTTCCAGCAGGTCAAGGAC
TTCCTGGCCAGTGTCATCGCACCCTTTGAAATCGGGCCGGATAAGGTCCAAGTAGGCCTG
ACTCAGTACAGCGGGGATGCTCAGACTGAGTGGGACCTGAACTCCCTCAGCACCAAGGAA
CAGGTGCTGGCAGCTGTGCGCCGCCTCCGCTACAAGGGGGGGAACACGTTCACAGGCCTT
GCCCTGACCCACGTGCTGGGGCAGAACCTGCAGCCGGCGGCTGGCCTCCGTCCAGAGGCA
GCCAAGGTGGTGATTCTGGTGACGGACGGCAAGTCCCAGGACGATGTGCACACTGCTGCC
CGTGTCCTCAAGGACCTGGGCGTGAACGTCTTCGCTGTGGGTGTGAAGAACGCCGATGAG
GCTGAGCTGAGGCTCCTGGCGTCCCCGCCGAGGGACATCACCGTCCACAGCGTGCTGGAC
TTCCTGCAGCTCGGCGCGCTGGCTGGCCTGCTCAGCCGTCTCATCTGCCAGAGGCTCCAG
GGTGGGAGCCCGCGGCAGGGCCCAGCAGCGGCTCCAGCCCTGGACACCCTCCCTGCCCCC
ACCAGCCTGGTCCTGAGCCAGGTGACCTCCTCCAGCATCCGCCTGTCCTGGACTCCAGCC
CCCCGGCACCCCCTCAAGTATCTGATCGTTTGGCGAGCCTCTAGAGGTGGCACCCCCAGG
GAGGTGGTGGTGGAGGGACCCGCCGCCTCCACGGAGCTGCACAACCTGGCCTCCCGCACA
GAGTACCTGGTCTCCGTGTTCCCCATCTATGAGGGCGGGGTTGGCGAAGGCCTGCGGGGC
CTGGTGACCACAGCACCTCTGCCTCCGCCCCGGGCGCTGACCCTGGCCGCAGTGACGCCC
AGAACCGTCCACCTCACCTGGCAGCCCTCGGCCGGGGCCACCCACTACCTGGTGCGATGT
TCTCCTGCTTCCCCCAAGGGTGAAGAGGAGGAGCGAGAGGTGCAGGTCGGGCGGCCCGAG
GTGCTGCTGGATGGCCTGGAACCTGGCAGGGACTATGAGGTCTCGGTGCAGAGCCTGCGA
GGCCCTGAGGGCAGCGAGGCCCGGGGCATCCGTGCCAGGACCCCCACCCTGGCCCCCCCG
AGACACCTGGGCTTCTCAGACGTGAGCCACGACGCGGCACGAGTGTTCTGGGAGGGTGCC
CCGAGGCCTGTGCGCCTGGTCAGGGTCACCTATGTGTCCAGCGAGGGTGGACACTCGGGG
CAGACAGAGGCTCCTGGGAACGCCACCTCGGCCACGCTGGGGCCTCTCTCTTCCTCCACC
ACCTACACTGTCCGTGTCACCTGCCTCTACCCTGGGGGTGGCTCCTCTACGCTGACTGGC
CGGGTGACCACCAAGAAAGCTCCCAGCCCAAGCCAGCTGTCCATGACGGAGCTGCCAGGG
GATGCAGTCCAGCTGGCGTGGGTGGCCGCAGCCCCGTCTGGCGTGCTTGTCTACCAGATC
ACGTGGACGCCCCTGGGAGAGGGGAAGGCTCACGAGATCTCTGTCCCAGGGAACCTCGGC
ACGGCCGTCCTGCCTGGCCTAGGGAGGCACACAGAGTACGACGTCACCATCTTGGCCTAC
TACAGGGACGGGGCCCGCAGTGACCCTGTGTCCCTCCGCTATACCCCCTCCACGGTGAGC
AGGAGCCCACCCTCCAACCTGGCCCTGGCCTCGGAGACCCCCGACAGCCTGCAGGTCAGC
TGGACGCCCCCGCTTGGCCGCGTGCTCCATTACTGGCTCACCTACGCCCCCGCCTCTGGC
TTGGGACCCGAGAAATCCGTCTCTGTGCCAGGAGCCAGGAGCCACGTGACACTGCCCGAC
CTGCAGGCAGCCACGAAGTACAGGGTCCTGGTCTCAGCTATCTATGCAGCAGGCAGGAGT
GAGGCTGTGTCTGCCACGGGCCAGACAGCCTGCCCAGCCCTCCGCCCTGACGGCTCCCTC
CCAGGGTTTGACCTGATGGTGGCCTTCAGCCTGGTGGAAAAGGCTTATGCGTCCATCCGG
GGCGTGGCCATGGAGCCCTCTGCCTTCGGTGGGACCCCGACCTTCACGCTCTTCAAGGAC
GCCCAGCTGACAAGACGGGTCAGTGACGTCTACCCAGCCCCCCTACCTCCAGAGCACACC
ATCGTCTTCCTTGTGCGCCTACTTCCCGAGACACCCCGTGAGGCCTTCGCGCTGTGGCAG
ATGACAGCCGAGGACTTCCAGCCCCTCCTTGGGGTTCTGCTGGATGCCGGGAAGAAGTCC
CTGACCTACTTCCACCGTGACCCCAGGGCTGCCTTGCAGGAGGCCACCTTCGACCCGCAG
GAAGTGAGGAAGATTTTCTTCGGGAGCTTCCACAAGGTGCACGTGGCTGTGGGCCGCTCC
AAGGTCAGGCTCTATGTGGACTGCCGGAAGGTGGCTGAGCGGCCCCTTGGGGAGATGGGC
AGCCCACCCGCTGCGGGCTTCGTCACGCTGGGGAGGCTGGCCAAGGCCAGGGGCCCCCGG
AGCAGTTCGGCCGCGTTTCAGCTCCAGATGCTGCAGATCGTGTGCAGTGACACCTGGGCC
GATGAGGACCGGTGCTGTGAGCTCCCTGCCTCGAGGGATGGAGAGACCTGCCCCGCCTTC
GTGTCTGCCTGTTCCTGTTCCTCAGAGACCCCTGGGCCCCCAGGACCTCAAGGACCCCCA
GGCCTCCCTGGGAGGAATGGCACCCCAGGAGAGCAGGGCTTCCCAGGGCCCAGGGGTCCA
CCAGGGGTCAAAGGAGAGAAGGGAGACCATGGGCTTCCAGGCTTGCAGGGCCACCCCGGC
CACCAGGGCATCCCCGGGAGAGTTGGCCTCCAGGGACCAAAGGGAATGAGAGGCCTGGAG
GGAACTGCTGGCCTGCCTGGACCCCCTGGCCCCAGGGGGTTCCAGGGCATGGCAGGGGCC
AGGGGCACTAGTGGAGAGCGAGGACCTCCAGGGACCGTGGGGCCCACAGGACTGCCAGGG
CCCAAAGGGGAACGAGGAGAGAAGGGCGAGCCGCAGTCCCTTGCCACCCTCTACCAGCTT
GTGAGCCAGGCCTCACACGTGTCAAAGTTCGACTCCTTCCACGAGAACACCAGGCCCCCC
ATGCCCATCTTGGAGCAGAAGCTGGAGCCGGGCACTGAGCCCCTGGGGTCCCCTGGCACC
CGCAGCAAGGCCCTGGTTCCTGGAGAATGGGGGCGTGGTGGCCGCCACCTTGAGGGCAGA
GGGGAGCCTGGAGCTGTTGGTCAGATGGGCAGCCCTGGGCAGCAGGGGGCTAGCACCCAG
GGCCTCTGGGAGTTG
Protein sequence predicted based on nucleotide sequence
MSSGDPAHLGLCLWLWLGATLGREQVQASGLLRLAVLPEDRLQMKWRESEGSGLGYLVQV KPMAGDSEQEVILTTKTPKATVGGLSPSKGYTLQIFELTGSGRFLLARREFVIEDLKSSS LDRSSQRPLGSGAPEPTPSHTGSPDPEQASEPQVAFTPSQDPRTPAGPQFRCLPPVPADM VFLVDGSWSIGHSHFQQVKDFLASVIAPFEIGPDKVQVGLTQYSGDAQTEWDLNSLSTKE QVLAAVRRLRYKGGNTFTGLALTHVLGQNLQPAAGLRPEAAKVVILVTDGKSQDDVHTAA RVLKDLGVNVFAVGVKNADEAELRLLASPPRDITVHSVLDFLQLGALAGLLSRLICQRLQ GGSPRQGPAAAPALDTLPAPTSLVLSQVTSSSIRLSWTPAPRHPLKYLIVWRASRGGTPR EVVVEGPAASTELHNLASRTEYLVSVFPIYEGGVGEGLRGLVTTAPLPPPRALTLAAVTP RTVHLTWQPSAGATHYLVRCSPASPKGEEEEREVQVGRPEVLLDGLEPGRDYEVSVQSLR GPEGSEARGIRARTPTLAPPRHLGFSDVSHDAARVFWEGAPRPVRLVRVTYVSSEGGHSG QTEAPGNATSATLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSQLSMTELPG DAVQLAWVAAAPSGVLVYQITWTPLGEGKAHEISVPGNLGTAVLPGLGRHTEYDVTILAY YRDGARSDPVSLRYTPSTVSRSPPSNLALASETPDSLQVSWTPPLGRVLHYWLTYAPASG LGPEKSVSVPGARSHVTLPDLQAATKYRVLVSAIYAAGRSEAVSATGQTACPALRPDGSL PGFDLMVAFSLVEKAYASIRGVAMEPSAFGGTPTFTLFKDAQLTRRVSDVYPAPLPPEHT IVFLVRLLPETPREAFALWQMTAEDFQPLLGVLLDAGKKSLTYFHRDPRAALQEATFDPQ EVRKIFFGSFHKVHVAVGRSKVRLYVDCRKVAERPLGEMGSPPAAGFVTLGRLAKARGPR SSSAAFQLQMLQIVCSDTWADEDRCCELPASRDGETCPAFVSACSCSSETPGPPGPQGPP GLPGRNGTPGEQGFPGPRGPPGVKGEKGDHGLPGLQGHPGHQGIPGRVGLQGPKGMRGLE GTAGLPGPPGPRGFQGMAGARGTSGERGPPGTVGPTGLPGPKGERGEKGEPQSLATLYQL VSQASHVSKFDSFHENTRPPMPILEQKLEPGTEPLGSPGTRSKALVPGEWGRGGRHLEGR GEPGAVGQMGSPGQQGASTQGLWEL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
