Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00867741
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 5286nts
Open reading frame: 1 to 5286
ATGCAATTTCAACTGACCCTTTTTTTGCACCTTGGGTGGCTCAGTTACTCAAAAGCTCAA
GATGACTGCAACAGGGGTGCCTGTCATCCCACCACTGGTGATCTCCTGGTGGGCAGGAAC
ACGCAGCTTATGGCTTCTTCTACCTGTGGGCTGAGCAGAGCCCAGAAATACTGCATCCTC
AGTTACCTGGAGGGGGAACAAAAATGCTTCATCTGTGACTCTAGATTTCCATATGATCCG
TATGACCAACCCAACAGCCACACCATTGAGAATGTCATTGTAAGTTTTGAACCAGACAGA
GAAAAGAAATGGTGGCAATCTGAAAATGGTCTTGATCATGTCAGCATCAGACTGGACTTA
GAGGCATTATTTCGGTTCAGCCACCTTATCCTGACCTTTAAGACTTTTCGGCCTGCTGCA
ATGTTAGTTGAACGTTCCACAGACTATGGACACAACTGGAAAGTGTTCAAATATTTTGCA
AAAGACTGTGCCACTTCCTTTCCTAACATCACATCTGGCCAGGCCCAGGGAGTGGGAGAC
ATTGTTTGTGACTCCAAATACTCGGATATTGAACCCTCAACAGGTGGAGAGGTTGTTTTA
AAAGTTTTGGATCCCAGTTTTGAAATTGAAAACCCTTATAGCCCCTACATCCAAGACCTT
GTGACATTGACAAACCTGAGGATAAACTTTACCAAGCTCCACACCCTTGGGGATGCTTTG
CTTGGAAGGAGGCAAAATGATTCCCTTGATAAATACTACTATGCTCTGTACGAGATGATT
GTTCGGGGAAGCTGCTTTTGCAATGGCCATGCTAGCGAATGTCGCCCTATGCAGAAGATG
CGGGGAGATGTTTTCAGCCCTCCTGGAATGGTTCACGGTCAGTGTGTGTGTCAGCACAAT
ACAGATGGTCCGAACTGTGAGAGATGCAAGGACTTCTTCCAGGATGCTCCTTGGAGGCCA
GCTGCAGACCTCCAGGACAACGCTTGCAGATCGTGCAGCTGTAATAGCCACTCCAGCCGC
TGTCACTTTGACATGACTACGTACCTGGCAAGCGGTGGCCTCAGCGGGGGCGTGTGTGAA
GACTGCCAGCACAACACTGAGGGGCAGCACTGCGACCGCTGCAGACCCCTCTTCTACAGG
GACCCGCTCAAGACCATCTCAGATCCCTACGCGTGCATTCCTTGTGAATGTGACCCCGAT
GGGACCATATCTGGTGGCATTTGTGTGAGCCACTCTGATCCTGCCTTAGGGTCTGTGGCC
GGCCAGTGCCTTTGTAAAGAGAACGTGGAAGGAGCCAAATGCGACCAGTGCAAACCCAAC
CACTACGGACTAAGCGCCACCGACCCCCTGGGCTGCCAGCCCTGCGACTGTAACCCCCTT
GGGAGTCTGCCATTCTTGACCTGTGATGTGGATACAGGCCAATGCTTGTGCCTGTCATAT
GTCACCGGAGCACACTGCGAAGAATGCACTGTTGGATACTGGGGCCTGGGAAATCATCTC
CATGGGTGTTCTCCCTGTGACTGTGATATTGGAGGTGCTTATTCTAACGTGTGCTCACCC
AAGAATGGGCAGTGTGAATGCCGCCCACATGTCACTGGCCGTAGCTGCTCTGAACCAGCC
CCTGGCTACTTCTTTGCTCCTTTGAATTTCTATCTCTACGAGGCAGAGGAAGCCACAACA
CTCCAAGGACTGGCGCCTTTGGGCTCGGAGACGTTTGGCCAGAGTCCTGCTGTTCACGTT
GTTTTAGGAGAGCCAGTTCCTGGGAACCCTGTTACATGGACTGGACCTGGATTTGCCAGG
GTTCTCCCTGGGGCTGGCTTGAGATTTGCTGTCAACAACATTCCCTTTCCTGTGGACTTC
ACCATTGCCATTCACTATGAAACCCAGTCTGCAGCTGACTGGACTGTCCAGATTGTGGTG
AACCCCCCTGGAGGGAGTGAGCACTGCATACCCAAGACTCTACAGTCAAAGCCTCAGTCT
TTTGCCTTACCAGCGGCTACGAGAATCATGCTGCTTCCCACACCCATCTGTTTAGAACCA
GATGTACAATATTCCATAGATGTCTATTTTTCTCAGCCTTTGCAAGGAGAGTCCCACGCT
CATTCACATGTCCTGGTGGACTCTCTTGGCCTTATTCCCCAAATCAATTCATTGGAGAAT
TTCTGCAGCAAGCAGGACTTAGATGAGTATCAGCTTCACAACTGTGTTGAAATTGCCTCA
GCAATGGGACCTCAAGTGCTCCCGGGTGCCTGTGAAAGGCTGATCATCAGCATGTCTGCC
AAGCTGCATGATGGGGCTGTGGCCTGCAAGTGTCACCCCCAGGGCTCAGTCGGATCCAGC
TGCAGCCGACTTGGAGGCCAGTGCCAGTGTAAACCTCTTGTGGTCGGGCGCTGCTGTGAC
AGGTGCTCAACTGGAAGCTATGATTTGGGGCATCACGGCTGTCACCCATGTCACTGCCAT
CCTCAAGGATCAAAGGACACTGTATGTGACCAAGTAACAGGACAGTGCCCCTGCCATGGA
GAGGTGTCTGGCCGCCGCTGTGATCGCTGCCTGGCAGGCTACTTTGGATTTCCCAGCTGC
CACCCTTGCCCTTGTAATAGGTTTGCTGAACTTTGTGATCCTGAGACAGGGTCATGCTTC
AATTGTGGAGGCTTTACAACTGGCAGAAACTGTGAAAGGTGTATTGATGGTTACTATGGA
AATCCTTCTTCAGGACAGCCCTGTCGTCCTTGCCTGTGTCCAGATGATCCCTCAAGCAAT
CAGTATTTTGCCCATTCCTGTTATCAGAATCTGTGGAGCTCAGATGTAATCTGCAATTGT
CTTCAAGGTTATACGGGTACTCAGTGTGGAGAATGCTCTACTGGTTTCTATGGAAATCCA
AGAATTTCAGGAGCACCTTGCCAACCATGTGCCTGCAACAACAACATAGATGTAACCGAT
CCAGAGTCCTGCAGCCGGGTAACAGGGGAGTGCCTTCGATGTTTGCACAACACTCAGGGC
GCAAACTGCCAGCTCTGCAAACCAGGTCACTATGGATCAGCCCTCAATCAGACCTGCAGA
AGATGCTCCTGCCATGCTTCCGGCGTGAGTCCCATGGAGTGTCCCCCTGGTGGGGGAGCT
TGCCTCTGTGACCCTGTCACTGGTGCATGTCCTTGTCTGCCGAATGTCACAGGCCTGGCC
TGTGACCGTTGTGCTGATGGATACTGGAATCTGGTCCCTGGCAGAGGATGTCAGTCATGT
GACTGTGACCCTAGGACCTCTCAAAGTAGCCACTGTGACCAGCTTACAGGCCAGTGTCCG
TGTAAATTAGGTTACGGCGGGAAACGTTGCAGTGAGTGCCAGGAAAATTATTATGGTGAT
CCACCTGGGCGATGCATTCCATGTGATTGTAACAGGGCAGGTACCCAGAAGCCCATCTGT
GATCCAGACACAGGCATGTGCCGCTGCCGGGAGGGTGTCAGCGGCCAGAGATGTGATCGC
TGTGCCCGGGGACACAGCCAGGAATTCCCTACTTGTCTTCAATGTCACTTGTGCTTTGAT
CAGTGGGACCACACCATTTCTTCCCTCTCCAAAGCGGTGCAAGGGTTAATGAGACTGGCT
GCTAACATGGAAGATAAAAGAGAGACCCTGCCTGTCTGTGAGGCAGACTTCAAAGACCTC
AGAGGGAACGTGTCTGAAATAGAAAGGATTTTGAAACATCCTGTTTTCCCATCTGGGAAA
TTCTTAAAAGTCAAGGATTATCATGACTCTGTTAGAAGACAAATCATGCAGCTAAATGAA
CAACTGAAAGCAGTGTATGAATTTCAAGATCTGAAAGATACAATAGAAAGAGCAAAGAAT
GAAGCAGACCTCTTACTTGAAGACCTTCAGGAAGAAATTGATTTGCAATCCAGTGTCCTT
AATGCAAGCATTGCGGACTCCTCAGAAAACATCAAGAAATATTATCACATATCATCATCT
GCTGAAAAGAAAATTAATGAAACTAGTTCCACCATTAATACCTCTGCAAATACAAGGAAT
GACTTACTTACCATCTTAGATACACTAACCTCAAAAGGAAACTTGTCATTGGAAAGATTA
AAGCAGATTAAGATACCAGATATCCAAATATTGAATGAAAAGGTGTGCGGAGATCCAGGA
AATGTGCCATGTGTGCCCTTGCCCTGTGGCGGTGCTCTCTGCACGGGCCGGAAGGGGCAC
AGGAAGTGTAGGGGTCCCGGCTGTCACGGCTCCCTGACCCTCTCAACGAATGCCCTCCAA
AAAGCCCAGGAAGCAAAATCCATTATTCGTAATTTGGACAAACAGGTTCGTGGGTTGAAA
AATCAGATCGAAAGTATAAGTGAACAGGCAGAAGTCTCCAAAAACAATGCCTTACAGCTG
AGGGAAAAACTGGGAAATATAAGAAACCAAAGTGACTCTGAAGAAGAAAACATCAATCTT
TTCATCAAAAAAGTGAAAAACTTTTTGTTAGAGGAAAACGTGCCTCCAGAAGACATCGAG
AAGGTTGCGAATGGTGTGCTTGACATTCACCTACCAATTCCATCCCAAAATCTAACCGAT
GAACTTGTCAAAATACAGAAACATATGCAACTCTGTGAGGATTACAGGACAGATGAAAAC
AGGTTAAATGAAGAAGCAGATGGAGCCCAAAAGCTTTTGGTGAAGGCCAAAGCAGCTGAG
AAAGCAGCAAATATTCTATTAAATCTTGACAAAACATTGAACCAGTTACAACAAGCTCAA
ATCACTCAAGGACGGGCAAACTCTACCATTACACAGCTGACTGCCAATATAACAAAAATA
AAAAAGAATGTGCTGCAGGCTGAAAATCAAACCAGGGAAATGAAGAGTGAGCTGGAGTTA
GCAAAGCAGCGATCAGGGCTGGAGGATGGACTTTCCCTGCTGCAGACCAAGTTGCAAAGG
CATCAAGACCACGCTGTCAATGCGAAAGTTCAGGCTGAATCTGCCCAACACCAGGCTGGG
AGTCTTGAGAAGGAATTTGTTGAGCTGAAAAAACAATATGCTATTCTCCAACGTAAGACA
AGCACTACAGGACTAACAAAGGAGACATTAGGAAAAGTTAAACAGCTAAAAGATGCGGCA
GAAAAATTGGCTGGAGATACAGAGGCCAAGATAAGAAGAATAACAGATTTAGAAAGGAAA
ATCCAAGATTTGAATCTAAGTAGACAAGCAAAAGCTGATCAACTGAGAATATTGGAAGAT
CAAGTTGTTGCCATTAAAAATGAAATTGTTGAACAAGAAAAAAAATATGCTAGGTGCTAT
AGCTTG
Protein sequence predicted based on nucleotide sequence
MQFQLTLFLHLGWLSYSKAQDDCNRGACHPTTGDLLVGRNTQLMASSTCGLSRAQKYCIL SYLEGEQKCFICDSRFPYDPYDQPNSHTIENVIVSFEPDREKKWWQSENGLDHVSIRLDL EALFRFSHLILTFKTFRPAAMLVERSTDYGHNWKVFKYFAKDCATSFPNITSGQAQGVGD IVCDSKYSDIEPSTGGEVVLKVLDPSFEIENPYSPYIQDLVTLTNLRINFTKLHTLGDAL LGRRQNDSLDKYYYALYEMIVRGSCFCNGHASECRPMQKMRGDVFSPPGMVHGQCVCQHN TDGPNCERCKDFFQDAPWRPAADLQDNACRSCSCNSHSSRCHFDMTTYLASGGLSGGVCE DCQHNTEGQHCDRCRPLFYRDPLKTISDPYACIPCECDPDGTISGGICVSHSDPALGSVA GQCLCKENVEGAKCDQCKPNHYGLSATDPLGCQPCDCNPLGSLPFLTCDVDTGQCLCLSY VTGAHCEECTVGYWGLGNHLHGCSPCDCDIGGAYSNVCSPKNGQCECRPHVTGRSCSEPA PGYFFAPLNFYLYEAEEATTLQGLAPLGSETFGQSPAVHVVLGEPVPGNPVTWTGPGFAR VLPGAGLRFAVNNIPFPVDFTIAIHYETQSAADWTVQIVVNPPGGSEHCIPKTLQSKPQS FALPAATRIMLLPTPICLEPDVQYSIDVYFSQPLQGESHAHSHVLVDSLGLIPQINSLEN FCSKQDLDEYQLHNCVEIASAMGPQVLPGACERLIISMSAKLHDGAVACKCHPQGSVGSS CSRLGGQCQCKPLVVGRCCDRCSTGSYDLGHHGCHPCHCHPQGSKDTVCDQVTGQCPCHG EVSGRRCDRCLAGYFGFPSCHPCPCNRFAELCDPETGSCFNCGGFTTGRNCERCIDGYYG NPSSGQPCRPCLCPDDPSSNQYFAHSCYQNLWSSDVICNCLQGYTGTQCGECSTGFYGNP RISGAPCQPCACNNNIDVTDPESCSRVTGECLRCLHNTQGANCQLCKPGHYGSALNQTCR RCSCHASGVSPMECPPGGGACLCDPVTGACPCLPNVTGLACDRCADGYWNLVPGRGCQSC DCDPRTSQSSHCDQLTGQCPCKLGYGGKRCSECQENYYGDPPGRCIPCDCNRAGTQKPIC DPDTGMCRCREGVSGQRCDRCARGHSQEFPTCLQCHLCFDQWDHTISSLSKAVQGLMRLA ANMEDKRETLPVCEADFKDLRGNVSEIERILKHPVFPSGKFLKVKDYHDSVRRQIMQLNE QLKAVYEFQDLKDTIERAKNEADLLLEDLQEEIDLQSSVLNASIADSSENIKKYYHISSS AEKKINETSSTINTSANTRNDLLTILDTLTSKGNLSLERLKQIKIPDIQILNEKVCGDPG NVPCVPLPCGGALCTGRKGHRKCRGPGCHGSLTLSTNALQKAQEAKSIIRNLDKQVRGLK NQIESISEQAEVSKNNALQLREKLGNIRNQSDSEEENINLFIKKVKNFLLEENVPPEDIE KVANGVLDIHLPIPSQNLTDELVKIQKHMQLCEDYRTDENRLNEEADGAQKLLVKAKAAE KAANILLNLDKTLNQLQQAQITQGRANSTITQLTANITKIKKNVLQAENQTREMKSELEL AKQRSGLEDGLSLLQTKLQRHQDHAVNAKVQAESAQHQAGSLEKEFVELKKQYAILQRKT STTGLTKETLGKVKQLKDAAEKLAGDTEAKIRRITDLERKIQDLNLSRQAKADQLRILED QVVAIKNEIVEQEKKYARCYSL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
