Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00868243
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4491nts
Open reading frame: 1 to 4491
ATGGCCCTGAGCAAAGGGCTGCGGCTGCTGGGGCGCCTGGGGGCCGAGGGGGACTGTAGC
GTGCTGCTGGAGGCGCGCGGCCGCGACGACTGCCTGCTGTTCGAGGCCGGCACGGTGGCC
ACGCTGGCTCCAGAAGAAAAGGAAGTCATTAAAGGACAGTATGGCAAGCTCACGGACGCG
TACGGCTGCCTGGGGGAGCTGAGGCTGAAATCTGGTGGCACGTCTCTGAGCTTCCTGGTG
TTGGTGACAGGCTGCACATCTGTGGGCAGAATTCCAGATGCTGAAATCTACAAAATCACT
GCCACTGACTTTTACCCTCTTCAGGAAGAGGCCAAGGAGGAGGAACGCCTCATAGCTTTG
AAGAAAATCCTCAGCTCGGGGGTGTTCTATTTCTCATGGCCAAACGATGGGTCTCGCTTT
GACCTGACTGTCCGCACGCAGAAGCAGGGGGATGACAGCTCTGAATGGGGGAACTCCTTC
TTCTGGAACCAGCTGTTGCACGTGCCCTTGAGGCAGCACCAGGTGAGCTGCTGTGACTGG
CTGCTGAAGATCATCTGCGGGGTGGTCACCATCCGCACCGTGTATGCCTCCCACAAGCAG
GCCAAGGCCTGCCTCGTCTCTCGCGTTAGCTGTGAGCGCACAGGCACTCGCTTCCACACC
CGTGGCGTGAACGACGACGGCCATGTGTCCAACTTCGTGGAGACAGAGCAGATGATTTAC
ATGGACGATGGAGTGTCATCTTTTGTCCAGATCAGAGGCTCCGTTCCGCTGTTCTGGGAA
CAGCCAGGGCTTCAGGTTGGCTCCCATCATCTGAGACTCCACAGAGGCCTGGAAGCCAAT
GCCCCTGCTTTCGACAGGCACATGGTGCTTCTGAAGGAGCAGTACGGGCAGCAGGTGGTC
GTGAACCTTCTGGGAAGCAGAGGCGGAGAGGAGGTGCTCAACAGAGCCTTCAAGAAGCTG
CTCTGGGCTTCTTGCCACGCGGGCGACACGCCTATGATCAATTTTGACTTCCATCAGTTT
GCCAAAGGTGGGAAGCTAGAGAAATTGGAGACCCTCTTGAGGCCACAGTTAAAGCTGCAC
TGGGAAGACTTCGATGTGTTCACAAAGGGGGAGAACGTCAGTCCACGTTTTCAGAAAGGC
ACTTTGCGGATGAACTGTCTTGACTGCCTGGACCGAACCAACACTGTGCAGAGCTTCATC
GCGCTCGAGGTCCTGCATCTGCAGCTCAAGACCCTGGGGCTGAGTTCAAAACCCATCGTT
GACCGCTTTGTGGAGTCCTTCAAAGCCATGTGGTCTCTGAATGGCCACAGCCTGAGCAAG
GTGTTCACAGGCAGCAGAGCCCTGGAAGGGAAGGCCAAGGTGGGGAAGCTGAAGGATGGA
GCCCGGTCCATGTCTCGAACCATCCAGTCCAACTTCTTCGACGGGGTGAAGCAGGAGGCC
ATCAAGCTGCTGCTGGTTGGGGACGTCTACGGCGAGGAGGTGGCAGACAAAGGGGGCATG
CTGCTGGACAGCACGGCGCTCCTGGTGACTCCCAGGATCCTGAAAGCTATGACTGAGCGT
CAGTCCGAATTCACAAATTTCAAGCGGATCCGGATTGCTATGGGGACCTGGAACGTGAAC
GGAGGAAAGCAGTTCCGGAGCAACGTGCTCAGGACGGCGGAGCTGACAGACTGGCTGCTC
GACTCGCCCCAGCTCTCGGGAGCTACCGACTCCCAGGATGACAGCAGCCCAGCTGACATA
TTTGCTGTGGGGTTTGAAGAGATGGTGGAATTGAGCGCAGGGAATATTGTCAATGCCAGT
ACTACCAACAAGAAGATGTGGGGTGAACAGCTTCAGAAAGCCATCTCACGCTCTCATAGA
TACATTCTGTTGACTTCGGCACAGCTGGTGGGCGTCTGTCTTTATATCTTTGTACGTCCA
TACCATGTCCCGTTCATCAGGGACGTAGCCATCGACACAGTGAAGACGGGCATGGGGGGC
AAGGCGGGGAACAAGGGCGCCGTCGGCATCCGCTTCCAGTTCCACAGCACCAGCTTCTGC
TTCATATGTAGTCACCTGACGGCCGGGCAGTCCCAGGTGAAGGAGCGGAATGAAGACTAC
AAGGAGATCACCCAGAAACTCTGCTTCCCAATGGGGAGAAATGTTTTTTCTCATGATTAT
GTATTTTGGTGTGGCGATTTCAACTACCGCATTGATCTTACTTATGAAGAAGTCTTCTAT
TTTGTTAAACGCCAAGACTGGAAGAAACTTCTGGAATTTGATCAACTACAGCTACAGAAA
TCAAGTGGAAAAATTTTTAAGGACTTTCACGAAGGAGCCATTAACTTTGGACCCACCTAC
AAGTATGACGTTGGCTCAGCCGCCTACGATACAAGCGACAAATGCCGCACCCCCGCCTGG
ACAGACAGGGTGCTGTGGTGGAGGAAGAAACATCCCTTTGATAAAACAGCTGGAGAACTC
AACCTTCTAGACAGTGATCTAGATGTTGACACCAAAGTCAGACACACCTGGTCTCCTGGT
GCCCTGCAGTATTATGGTCGTGCGGAGCTACAAGCGTCTGATCACAGACCTGTGCTGGCG
ATCGTGGAGGTGGAAGTTCAGGAAGTCGATGTGGGTGCTCGGGAGAGGGTTTTCCAGGAA
GTGTCCTCCTTCCAGGGCCCCCTGGATGCCACTGTTGTAGTAAACCTTCAATCACCGACC
TTAGAAGAGAAAAACGAGTTTCCAGAGGACCTGCGTACTGAGCTCATGCAGACCTTGGGG
AGTTATGGGACAATTGTTCTTGTCAGGATCAACCAAGGGCAGATGCTGGTAACTTTTGCA
GACAGTCACTCGGCTCTCAGTGTCCTGGACGTGGACGGTATGAAGGTGAAAGGCAGAGCA
GTGAAGATTAGACCGAAGACCAAGGACTGGCTGAAAGGTTTGCGAGAGGAGATCATTCGG
AAACGAGACAGCATGGCCCCCGTGTCTCCCACTGCCAACTCCTGTTTGCTGGAGGAAAAC
TTTGACTTCACAAGTTTGGACTATGAGTCAGAAGGGGATATTCTTGAAGACGATGAAGAC
TACTTGGTGGATGAATTCAATCAGCCTGGAGTCTCGGACAGTGAACTCGGGGGAGACGAC
CTCTCTGATGTCCCCGGCCCCACAGCACTGGCTCCTCCCAGCAAGTCACCTGCTCTCACC
AAAAAGAAGCAGCATCCAACGTACAAAGATGACGCGGACCTGGTGGAGCTCAAGCGGGAG
CTGGAAGCCGTCGGGGAGTTCCGCCACCGTTCTCCGAGCAGGTCTCTGTCGGTCCCCAAC
CGGCCTCGGCCACCTCAACCCCCGCAGAGACCCCCCCCTCCAACCGGTTTAATGGTGAAA
AAGTCGGCTTCAGATGCGTCCATCTCCTCCGGCACCCATGGACAGTATTCAATTTTGCAG
ACGGCAAGACTTCTACCAGGAGCACCTCAGCAACCTCCCAAGGCTCGGACTGGAATAAGT
AAACCTTATAATGTCAAGCAGATCAAAACCACCAATGCCCAGGAGGCAGAAGCAGCAATC
CGGTGTCTCCTGGAAGCCAGAGGAGGTGCCTCCGAAGAAGCCCTAAGTGCCGTGGCCCCA
AGGGACCTTGAAGCATCCTCTGAACCAGAGCCCACACCGGGGGCAGCCAAACCAGAGACC
CCACAGGCGCCCCCACTCCTTCCCCGTCGGCCCCCACCCAGAGTTCCTGCCATCAAGAAG
CCAACCTTGAGAAGGACAGGAAAGCCCCTGTCACCGGAAGAACAGTTTGAGCAACAGACT
GTCCATTTTACAATCGGGCCCCCGGAGACAAGCGTTGAGGCCCCTCCTGTCGTGACAGCC
CCTCGAGTCCCTCCTGTTCCCAAACCAAGAACATTTCAGCCTGGGAAAGCTGCAGAGAGG
CCAAGCCACAGGAAGCCAGCATCAGACGAAGCCCCTCCTGGGGCAGGAGCCTCTGTGCCA
CCACCTCTGGAGGCGCCGCCTCTTGTGCCCAAGGTACCCCCGAGGAGGAAGAAGTCAGCC
CCCGCAGCCTTCCACCTGCAGGTCCTGCAGAGCAACAGCCAGCTTCTCCAGGGCCTCACT
TACAATAGCAGTGACAGCCCCTCTGGGCACCCACCTGCCGCGGGCACCGTCTTCCCACAA
GGGGACTTTCTCAGCACTTCATCTGCTACAAGCCCCGACAGCGATGGCACCAAAGCGATG
AAGCCAGAGGCAGCCCCACTTCTTGGTGATTATCAGGACCCCTTCTGGAACCTTCTTCAC
CACCCTAAACTGTTGAATAACACTTGGCTTTCTAAGAGCTCAGACCCTTTGGACTCAGGA
ACCAGGAGCCCCAAAAGAGATCCCATAGACCCAGTGTCAGCTGGCGCTTCAGCTGCCAAG
GCAGAGCTGCCACCAGATCATGAACACAAAACCTTAGGTCACTGGGTGACAATCAGTGAC
CAAGAAAAGAGGACAGCACTGCAGGTGTTTGACCCACTGGCAAAAACATTG
Protein sequence predicted based on nucleotide sequence
MALSKGLRLLGRLGAEGDCSVLLEARGRDDCLLFEAGTVATLAPEEKEVIKGQYGKLTDA YGCLGELRLKSGGTSLSFLVLVTGCTSVGRIPDAEIYKITATDFYPLQEEAKEEERLIAL KKILSSGVFYFSWPNDGSRFDLTVRTQKQGDDSSEWGNSFFWNQLLHVPLRQHQVSCCDW LLKIICGVVTIRTVYASHKQAKACLVSRVSCERTGTRFHTRGVNDDGHVSNFVETEQMIY MDDGVSSFVQIRGSVPLFWEQPGLQVGSHHLRLHRGLEANAPAFDRHMVLLKEQYGQQVV VNLLGSRGGEEVLNRAFKKLLWASCHAGDTPMINFDFHQFAKGGKLEKLETLLRPQLKLH WEDFDVFTKGENVSPRFQKGTLRMNCLDCLDRTNTVQSFIALEVLHLQLKTLGLSSKPIV DRFVESFKAMWSLNGHSLSKVFTGSRALEGKAKVGKLKDGARSMSRTIQSNFFDGVKQEA IKLLLVGDVYGEEVADKGGMLLDSTALLVTPRILKAMTERQSEFTNFKRIRIAMGTWNVN GGKQFRSNVLRTAELTDWLLDSPQLSGATDSQDDSSPADIFAVGFEEMVELSAGNIVNAS TTNKKMWGEQLQKAISRSHRYILLTSAQLVGVCLYIFVRPYHVPFIRDVAIDTVKTGMGG KAGNKGAVGIRFQFHSTSFCFICSHLTAGQSQVKERNEDYKEITQKLCFPMGRNVFSHDY VFWCGDFNYRIDLTYEEVFYFVKRQDWKKLLEFDQLQLQKSSGKIFKDFHEGAINFGPTY KYDVGSAAYDTSDKCRTPAWTDRVLWWRKKHPFDKTAGELNLLDSDLDVDTKVRHTWSPG ALQYYGRAELQASDHRPVLAIVEVEVQEVDVGARERVFQEVSSFQGPLDATVVVNLQSPT LEEKNEFPEDLRTELMQTLGSYGTIVLVRINQGQMLVTFADSHSALSVLDVDGMKVKGRA VKIRPKTKDWLKGLREEIIRKRDSMAPVSPTANSCLLEENFDFTSLDYESEGDILEDDED YLVDEFNQPGVSDSELGGDDLSDVPGPTALAPPSKSPALTKKKQHPTYKDDADLVELKRE LEAVGEFRHRSPSRSLSVPNRPRPPQPPQRPPPPTGLMVKKSASDASISSGTHGQYSILQ TARLLPGAPQQPPKARTGISKPYNVKQIKTTNAQEAEAAIRCLLEARGGASEEALSAVAP RDLEASSEPEPTPGAAKPETPQAPPLLPRRPPPRVPAIKKPTLRRTGKPLSPEEQFEQQT VHFTIGPPETSVEAPPVVTAPRVPPVPKPRTFQPGKAAERPSHRKPASDEAPPGAGASVP PPLEAPPLVPKVPPRRKKSAPAAFHLQVLQSNSQLLQGLTYNSSDSPSGHPPAAGTVFPQ GDFLSTSSATSPDSDGTKAMKPEAAPLLGDYQDPFWNLLHHPKLLNNTWLSKSSDPLDSG TRSPKRDPIDPVSAGASAAKAELPPDHEHKTLGHWVTISDQEKRTALQVFDPLAKTL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
