Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00877426
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4410nts
Open reading frame: 1 to 4410
ATGGATGTAGATGCTGAAAGAGAGAAGATAACACAGGAGATCAAGGAGCTGGAAAGGATT
TTGGATCCCGGCTCCTCGGGCTCCCACGTGGAGATCTCAGAATCAAGTCTCGAGTCAGAT
TCTGAAGCAGATTCACTGCCTTCTGAGGACTTGGATCCTGCCGATCCCCCGATCTCGGAA
GAAGAAAGGTGGGGCGAAGCCAGCAATGACGAGGACGATCCCAAGGATAAAACCCTCCCT
GAAGACCCAGAAACCTGCCTGCAGCTGAACATGGTCTACCAGGAGGTCATCCAGGAGAAG
CTGGCTGAGGCCAACCTGCTGCTGGCCCAGAACCGGGAGCAGCAGGAGGAACTCATGAGG
GATCTGGCTGGGTCCAAAGGCACCAAGGTGAAAGATGGCAAAAGCCTGCCCCCAAGCACA
TACATGGGGCACTTCATGAAGCCGTATTTCAAGGACAAGGTCACGGGCGTGGGGCCACCT
GCCAACGAGGACACACGAGAGAAGGCTGCCCAGGGGATCAAGGCTTTCGAGGAGCTCCTT
GTGACCAAATGGAAAAACTGGGAAAAGGCCTTGCTCCGAAAGTCAGTGGTGAGTGACCGC
CTGCAGCGATTGCTTCAGCCCAAGTTACTGAAGCTCGAGTACTTGCACCAGAAGCAGAGC
AAAGTCTCCAGTGAGCTGGAGAGGCAAGCCCTGGAGAAGCAGGGCAGGGAAGCCGAGAAG
GAGATCCAGGACATCAACCAGCTTCCAGAAGAGGCCTTGCTGGGAAACAGGCTGGACAGC
CACGACTGGGAGAAGATTTCCAATATTAACTTTGAAGGCAGCCGCAGTGCAGAGGAGATC
CGGAAGTTCTGGCAGAACTCGGAGCACCCCAGCATCAACAAGCAGGAGTGGAGCAGGGAG
GAGGAGGAGCGGCTGCAGGCGATCGCGGCTGCACACGGCCACCTGGAGTGGCAGAAGATT
GCAGAGGAGCTGGGGACCAGCCGCAGCGCCTTCCAGTGCCTGCAGAAATTCCAGCAGCAC
AACAAAGCTCTGAAACGCAAGGAGTGGACAGAGGAGGAGGACCGCATGCTCACGCAGCTG
GTGCAGGAGATGCGCGTCGGCAGCCACATCCCCTACCGCAGAATTGTCTACTATATGGAA
GGGAGAGACTCCATGCAGCTGATCTACCGATGGACCAAGAGCTTGGATCCTGGTCTGAAG
AAGGGTTACTGGGCCCCGGAGGAAGATGCTAAGTTGCTTCAAGCTGTTGCCAAATACGGG
GAGCAGGATTGGTTTAAAATCCGGGAAGAGGTGCCAGGTAGGAGCGATGCCCAGTGCCGA
GATCGGTATCTCAGGAGATTACATTTCAGCTTGAAAAAGGGTCGGTGGAATTTAAAAGAA
GAGGAACAGTTAATTGAATTAATAGAAAAATATGGTGTCGGTCACTGGGCAAAAATAGCT
TCTGAGCTGCCCCATCGGTCTGGCTCCCAGTGTCTGAGCAAGTGGAAGATCATGATGGGG
AAGAAGCAGGGTCTCCGGAGGCGGCGGCGGAGGGCCCGTCACAGCGTCCGGTGGAGCTCT
ACCAGCAGCAGCGGCAGCAGCAGTGGCAGCAGTGGAGGGAGCAGCAGCAGCAGCAGCAGC
AGCAGCGAGGAGGACGAGCCAGAGCAGGCGCAGGCCGGGGAGGGTGACAGAGCGCTGCTG
TCCCCACAGTACATGGTCCCGGACATGGACCTGTGGGTTCCTGCCAGGCAGAGCACCAGC
CAGCCATGGAGAGGAGGGGCAGGGGCCTGGCTGGGAGGCCCCGCTGCCTCCCTCAGCCCT
CCCAAGGGGTCCAGTGCCAGCCAGGGCGGCAGCAAGGAAGCTTCCACCACAGCCGCGGCT
CCTGGAGAGGAGACGAGTCCGGTGCAGGTCCCTGCCAGGGCCCACGGCCCTGTCCCGAGG
TCTGCCCAGGCCTCCCACTCAGCAGACACTCGCCCGGCGGGCGCAGAGAAGCAGGCCCTG
GAGGGTGGGAGGCGTCTGCTGACAGTGCCTGTGGAGACCGTGCTGAGGGTGCTCAGGGCC
AACACGGCTGCTCGGAGCTGCACACAGAAAGAGCAGCTGAGGCAGCCACCCCTGCCCACC
TCATCCCCAGGGGTCAGCTCTGGTGACAGCGTGGCCCGATCCCATGTGCAGTGGCTACGG
CACAGAGCCACCCAGAGTGGGCAGCGGCGCTGGAGACACGCTCTGCACCGGAGGCTCCTG
AACCGCAGGCTGCTGCTGGCTGTGACCCCTTGGGTAGGGGACGTTGTCGTGCCCTGCACA
CAGGCTTCCCAGAGACCCGCCGTAGTGCAGACTCAAGCGGATGGCCTCAGGGAGCAGCTG
CAGCAGGCCCGCCTGGCCAGCACCCCTGTGTTTACCCTGTTTACCCAGCTGTTCCACATC
GATACTGCCGGCTGCTTGGAGGTCGTCCGAGAGAGGAAGGCCCTGCCACCCAGGCTGCCC
CAGGCTGGTGCTCGGGACCCACCAGTTCATCTTCTGCAGGCATCCTCAAGTGCCCAGAGC
ACCCCAGGCCACCTCTTCCCAAACGTGCCGGCTCAAGAAGCCTCAAAGAGTGCCAGCCAC
AAAGGGAGCCGAAGACTGGCGTCCAGCCGGGTGGAGCGCACCCTACCCCAGGCGTCCCTG
CTGGCTTCAACCGGCCCCCGGCCCAAGCCCAAGACTGTGTCGGAGCTGCTTCAGGAGAAG
CGGCTTCAGGAGGCCCGTGCCAGGGAGGCCACCCGGGGCCCGGTGGTGCTCCCGTCCCAG
CTGCTGGTCTCCTCGTCTGTGATCCTCCAGCCCCCTCTACCACACACCCCACACGGCCGC
CCAGCCCCGGGTCCCACCGTCTTAAATGTACCGCTCTCTGGGCCTGGGGCCCCCGCGGCA
GCCAAACCTGGCACTTCTGGCTCCTGGCAGGAGGCTGGGACTTCAGCCAAGGACAAGAGA
CTCTCCACCATGCAAGCCCTGCCCCTCGCTCCTGTCTTCTCAGAGGCCGAGGGCACAGCC
CCTGCTGCTTCCCAAGCCCCTGCCCTGGGCCCCGGCCAGATCTCTGTGAGCTGCCCCGAG
AGTGGTCTCGGACAGTCTCAGGCCCCCGCTGCATCCCGGAAGCAGGGCCTGCCTGAGGCG
CCACCCTTTCTCCCCGCAGCCCCCAGCCCCACCCCACTGCCCGTCCAGCCCCTCAGCCTG
ACGCACATAGGAGGGCCACATGTGGCGACCAGTGTCCCCCTGCCTGTCACCTGGGTGCTC
ACAGCCCAGGGGCTTCTCCCTGTTCCTGTACCAGCTGTGGTGAGCCTTCCCAGGCCAGCA
GGGACCCCTGGCCCCGCAGGGCTGCTGGCCACTCTGCTGCCTCCCCTGACTGAGACTCGG
GCGGCCCAGGGCCCCAGGGCCCCAGCGTTGAGCAGCTCTTGGCAGCCCCCAGCCAATATG
AACAGGGAACCGGAGCCTTCCTGCAGGACAGACACCCCAGCTCCTCCCACACACGCCCTC
TCCCAAAGTCCTGCAGAAGCGGATGGCAGTGTGGCCTTTGTCCCTGGAGAGGCCCAGGTG
GCCAGGGAGATACCTGAGCCCAGGACGTCCTCCCACGCTGACCCTCCTGAAGCAGAACCC
CCTTGGTCCGGGAGGCTGCCAGCCTTCGGTGGTGTCATCCCAGCAACTGAGCCAAGGGGG
ACGCCGGGGTCCCCCTCAGGGACACAGGAGCCCAGGGGGCCTCTGGGCCTGGAGAAGCTG
CCCCTGCGCCAGCCTGGGCCTGAGAAGGGGGCCCTGGACCTGGAGAAGCCGCCCCTACCC
CAGCCTGGGCCTGAGAAGGGGGCCCTGGACCTGGGCCTGCTGTCCCAGGAGGGCGAGGCG
GCCACACAGCAGTGGCTGGGGGGCCAGCGGGGGGTGCGTGTGCCTCTTCTGGGCAGCAGA
CTGCCCTATCAGCCCCCAGCCCTGTGCAGCCTGCGAGCTCTGTCCGGTCTCCTACTCCAC
AAGAAGGCCCTGGAGCACAAGGCCACCTCCCTGGTGGTGGGGGGCGAGGCTGAGCGGCCG
GCCGGAGCACTGCAAGCCTCACTGGGGCTGGTGCGGGGGCAGCTCCAGGACAACCCGGCC
TACCTCCTGTTGCGGGCGCGGTTCCTGGCAGCCTTCACCCTCCCTGCGCTCCTGGCCACC
CTGGCCCCCCAAGGCGTCCGCACCACCCTCTCAGTACCTTCGAGGGTGGGCTCTGAGAGT
GAGGATGAAGACCTCCTGAGTGAGCTGGAACTTGCAGACAGGGACGGGCAGCCGGGCTGC
ACGACAGCCACATGCCCCATTCAGGGAGCCCCAGACTCTGGTAAATGCTCTGCTTCCTCC
TGCCTGGATACTTCTAATGACCCTGACGACCTGGACGTGCTCAGAACCCGGCATGCCAGG
CACACCCGGAAGCGGAGGCGGCTGGTGTTG
Protein sequence predicted based on nucleotide sequence
MDVDAEREKITQEIKELERILDPGSSGSHVEISESSLESDSEADSLPSEDLDPADPPISE EERWGEASNDEDDPKDKTLPEDPETCLQLNMVYQEVIQEKLAEANLLLAQNREQQEELMR DLAGSKGTKVKDGKSLPPSTYMGHFMKPYFKDKVTGVGPPANEDTREKAAQGIKAFEELL VTKWKNWEKALLRKSVVSDRLQRLLQPKLLKLEYLHQKQSKVSSELERQALEKQGREAEK EIQDINQLPEEALLGNRLDSHDWEKISNINFEGSRSAEEIRKFWQNSEHPSINKQEWSRE EEERLQAIAAAHGHLEWQKIAEELGTSRSAFQCLQKFQQHNKALKRKEWTEEEDRMLTQL VQEMRVGSHIPYRRIVYYMEGRDSMQLIYRWTKSLDPGLKKGYWAPEEDAKLLQAVAKYG EQDWFKIREEVPGRSDAQCRDRYLRRLHFSLKKGRWNLKEEEQLIELIEKYGVGHWAKIA SELPHRSGSQCLSKWKIMMGKKQGLRRRRRRARHSVRWSSTSSSGSSSGSSGGSSSSSSS SSEEDEPEQAQAGEGDRALLSPQYMVPDMDLWVPARQSTSQPWRGGAGAWLGGPAASLSP PKGSSASQGGSKEASTTAAAPGEETSPVQVPARAHGPVPRSAQASHSADTRPAGAEKQAL EGGRRLLTVPVETVLRVLRANTAARSCTQKEQLRQPPLPTSSPGVSSGDSVARSHVQWLR HRATQSGQRRWRHALHRRLLNRRLLLAVTPWVGDVVVPCTQASQRPAVVQTQADGLREQL QQARLASTPVFTLFTQLFHIDTAGCLEVVRERKALPPRLPQAGARDPPVHLLQASSSAQS TPGHLFPNVPAQEASKSASHKGSRRLASSRVERTLPQASLLASTGPRPKPKTVSELLQEK RLQEARAREATRGPVVLPSQLLVSSSVILQPPLPHTPHGRPAPGPTVLNVPLSGPGAPAA AKPGTSGSWQEAGTSAKDKRLSTMQALPLAPVFSEAEGTAPAASQAPALGPGQISVSCPE SGLGQSQAPAASRKQGLPEAPPFLPAAPSPTPLPVQPLSLTHIGGPHVATSVPLPVTWVL TAQGLLPVPVPAVVSLPRPAGTPGPAGLLATLLPPLTETRAAQGPRAPALSSSWQPPANM NREPEPSCRTDTPAPPTHALSQSPAEADGSVAFVPGEAQVAREIPEPRTSSHADPPEAEP PWSGRLPAFGGVIPATEPRGTPGSPSGTQEPRGPLGLEKLPLRQPGPEKGALDLEKPPLP QPGPEKGALDLGLLSQEGEAATQQWLGGQRGVRVPLLGSRLPYQPPALCSLRALSGLLLH KKALEHKATSLVVGGEAERPAGALQASLGLVRGQLQDNPAYLLLRARFLAAFTLPALLAT LAPQGVRTTLSVPSRVGSESEDEDLLSELELADRDGQPGCTTATCPIQGAPDSGKCSASS CLDTSNDPDDLDVLRTRHARHTRKRRRLVL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
