Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00878310
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 8772nts
Open reading frame: 1 to 8772
ATGCGGAGCCCGGCCACCGGCGTCCCCCTCCCAACGCCGCCGCCGCCGCTGCTGCTGCTG
TTGCTGCTGCTGCTGCCGCCGCCACTATTGGGAGACCAAGTGGGGCCCTGTCGTTCCTTG
GGGTCCAGGGGACGAGGCTCTTCGGGGGCCTGCGCCCCCATGGGCTGGCTCTGTCCATCC
TCAGCGTCGAACCTCTGGCTCTACACCAGCCGCTGCAGGGATGCGGGCACTGAGCTGACT
GGCCACCTGGTACCCCACCACGATGGCCTGAGGGTTTGGTGTCCAGAATCCGAGGCCCAT
ATTCCCCTACCACCAGCTCCTGAAGGCTGCCCCTGGAGCTGTCGCCTCCTGGGCATTGGA
GGCCACCTTTCCCCACAGGGCAAGCTCACACTGCCCGAGGAGCACCCGTGCTTAAAGGCT
CCACGGCTCAGATGCCAGTCCTGCAAGCTGGCACAGGCCCCCGGGCTCAGGGCAGGGGAA
AGGTCACCAGAAGAGTCCCTGGGTGGGCGTCGGAAAAGGAATGTAAATACAGCCCCCCAG
TTCCAGCCCCCCAGCTACCAGGCCACAGTGCCGGAGAACCAGCCAGCAGGCACCCCTGTT
GCATCCCTGAGGGCCATCGACCCGGACGAGGGTGAGGCAGGTCGACTGGAGTACACCATG
GATGCCCTCTTTGATAGCCGCTCCAACCAGTTCTTCTCCCTGGACCCAGTCACTGGTGCA
GTAACCACAGCCGAGGAGCTGGATCGTGAGACCAAGAGCACCCACGTCTTCAGGGTCACG
GCGCAGGACCACGGCATGCCCCGACGAAGTGCCCTGGCTACACTCACCATCTTGGTTACT
GACACCAATGACCATGACCCTGTGTTCGAGCAGCAGGAGTACAAGGAGAGCCTCAGGGAG
AACCTGGAGGTTGGCTATGAGGTGCTCACTGTCAGGGCCACGGATGGTGATGCCCCTCCC
AATGCCAATATTCTGTACCGCCTGCTGGAGGGGTCTGGGGGCAGCCCCTCTGAAGTCTTT
GAGATCGACCCTCGCTCTGGGGTGATCCGAACCCGTGGCCCTGTGGATCGGGAAGAGGTG
GAATCCTACCAGCTGACGGTAGAGGCAAGTGACCAGGGTCGGGACCCGGGTCCTCGGAGT
ACCACAGCCGCTGTTTTCCTTTCTGTGGAGGATGACAATGATAATGCCCCCCAGTTTAGT
GAGAAGCGCTATGTGGTCCAGGTGAGGGAGGATGTGACTCCAGGGGCCCCAGTACTCCGA
GTCACAGCCTCGGATCGAGACAAGGGGAGCAATGCCGTGGTGCACTATAGCATCATGAGT
GGCAATGCTCGGGGACAGTTTTATCTGGATGCCCAGACTGGAGCTCTGGATGTGGTGAGC
CCTCTTGACTATGAGACGACCAAGGAGTACACCCTACGGGTGCGAGCACAGGATGGTGGC
CGTCCCCCACTCTCTAATGTCTCTGGCTTGGTGACAGTACAGGTCCTGGATATCAACGAC
AATGCCCCCATCTTCGTCAGCACCCCTTTCCAGGCTACTGTCCTGGAGAGTGTCCCCTTA
GGCTACCTGGTTCTCCATGTCCAGGCTATCGACGCTGATGCTGGTGACAATGCCCGCCTG
GAATACCGCCTTGCTGGGGTGGGACATGACTTCCCCTTCACCATCAACAATGGCACAGGC
TGGATCTCTGTGGCTGCTGAACTGGACCGGGAGGAAGTTGATTTCTACAGCTTTGGGGTA
GAAGCTCGAGACCATGGCACTCCAGCACTCACTGCCTCGGCCAGTGTCAGCGTGACTGTC
CTGGATGTCAACGACAACAATCCAACCTTTACCCAACCAGAGTACACAGTGCGGCTCAAT
GAGGATGCAGCTGTGGGCACCAGCGTGGTGACGGTGTCAGCTGTGGACCGTGATGCTCAT
AGTGTCATCACCTACCAGATCACCAGTGGCAATACTCGAAACCGCTTCTCCATCACCAGC
CAAAGTGGTGGTGGGCTGGTATCCCTTGCCCTGCCACTGGACTACAAACTTGAGCGGCAG
TATGTGTTGGCTGTTACCGCCTCCGATGGCACTCGGCAGGACACGGCACAGATTGTGGTG
AATGTCACCGACGCCAACACCCATCGTCCTGTCTTTCAGAGCTCCCACTATACAGTGAAT
GTTAATGAGGACCGGCCGGCAGGCACCACGGTGGTGCTGATCAGCGCCACGGATGAGGAC
ACAGGTGAGAATGCCCGCATCACCTACTTCATGGAGGACAGCATCCCCCAGTTCCGCATC
GATGCAGACACGGGGGCTGTCACCACCCAGGCTGAGCTGGACTATGAAGACCAAGTGTCT
TACACCCTGGCCATTACTGCTCGGGACAATGGCATTCCCCAGAAGTCCGACACCACCTAC
CTGGAGATCCTGGTGAACGACGTGAATGACAATGCCCCTCAGTTCCTGCGAGACTCCTAC
CAGGGCAGTGTCTATGAGGATGTGCCACCCTTCACTAGCGTCCTGCAGATCTCAGCCACT
GATCGTGATTCTGGACTTAATGGCAGGGTCTTCTACACCTTCCAAGGAGGCGACGATGGA
GACGGTGACTTTATTGTTGAGTCCACGTCAGGCATCGTGCGAACGCTACGGAGGCTGGAT
CGAGAGAACGTGGCCCAGTATGTCTTGCGGGCATATGCAGTGGACAAGGGGATGCCCCCA
GCCCGCACACCTATGGAAGTGACAGTCACTGTGTTGGATGTGAATGACAATCCCCCTGTC
TTTGAGCAGGATGAGTTTGATGTGTTTGTGGAAGAGAACAGCCCCATTGGGCTAGCCGTG
GCCCGGGTCACAGCCACTGACCCCGATGAAGGCACCAATGCCCAGATTATGTACCAGATT
GTGGAGGGCAACATCCCTGAGGTCTTTCAGCTGGACATCTTCTCCGGGGAGCTGACAGCC
CTGGTAGACTTAGACTACGAGGACCGGCCTGAGTACGTCCTGGTCATCCAGGCCACGTCA
GCTCCTCTGGTGAGCCGGGCTACAGTCCACGTCCGCCTCCTTGACCGCAATGACAACCCA
CCAGTGCTGGGCAACTTTGAGATCCTTTTCAACAACTATGTCACCAATCGCTCAAGCAGC
TTCCCTGGGGGTGCCATTGGCCGAGTACCTGCCCATGACCCTGATATCTCAGATAGTCTG
ACTTACAGCTTTGAGCGGGGAAATGAACTCAGCCTGGTCCTGCTCAATGCCTCCACGGGT
GAGCTGAAGCTAAGCCGCGCACTGGACAACAACCGGCCTCTGGAGGCCATCATGAGCGTG
CTGGTGTCAGACGGCGTACACAGCGTGACCGCCCAGTGCGCGCTGCGTGTGACCATCATC
ACCGATGAGATGCTCACCCACAGCATCACGCTGCGCCTGGAGGACATGTCACCCGAGCGC
TTCCTGTCACCACTGCTAGGCCTCTTCATCCAGGCGGTGGCCGCCACGCTGGCCACGCCA
CCGGACCACGTGGTGGTCTTCAACGTACAGCGGGACACCGACGCCCCCGGGGGCCACATC
CTCAACGTGAGCCTGTCGGTGGGCCAGCCGCCAGGGCCCGGGGGCGGGCCGCCCTTCCTG
CCCTCTGAGGACCTGCAGGAGCGCCTATACCTCAACCGCAGCCTGCTGACGGCCATCTCG
GCACAGCGCGTGCTGCCCTTCGACGACAACATCTGCCTGCGGGAGCCCTGCGAGAACTAC
ATGCGCTGCGTGTCGGTGCTGCGCTTCGACTCCTCCGCGCCCTTCATCGCCTCCTCCTCC
GTGCTCTTCCGGCCCATCCACCCCGTCGGAGGGCTGCGCTGCCGCTGCCCGCCCGGCTTC
ACGGGTGACTACTGCGAGACCGAGGTGGACCTCTGCTACTCGCGGCCCTGTGGCCCCCAC
GGGCGCTGCCGCAGCCGCGAGGGCGGCTACACCTGCCTCTGTCGTGATGGCTACACGGGT
GAGCACTGTGAGGTGAGTGCTCGCTCAGGCCGTTGCACCCCGGGTGTCTGCAAGAATGGG
GGCACCTGTGTCAACCTGCTGGTGGGCGGTTTCAAGTGCGATTGCCCATCTGGAGACTTC
GAGAAGCCCTACTGCCAGGTGACCACGCGCAGCTTCCCCGCCCACTCCTTCATCACCTTT
CGCGGCCTGCGCCAGCGTTTCCACTTCACCCTGGCCCTCTCGTTTGCCACAAAGGAGCGC
GACGGGTTGCTGTTGTACAATGGGCGTTTCAATGAGAAGCATGACTTTGTGGCCCTCGAG
GTGATCCAGGAGCAGGTCCAGCTCACCTTCTCTGCAGGGGAGTCAACCACCACGGTGTCC
CCATTCGTGCCCGGAGGAGTCAGTGATGGCCAGTGGCATACGGTGCAGCTGAAATACTAC
AATAAGCCACTGTTGGGTCAGACAGGGCTCCCACAGGGCCCATCAGAGCAGAAGGTGGCT
GTGGTGACCGTGGATGGCTGTGACACAGGAGTGGCCTTGCGCTTCGGATCTGTCCTGGGC
AACTACTCCTGTGCTGCCCAGGGCACCCAGGGTGGCAGCAAGAAGTCTCTGGATCTGACG
GGGCCCCTGCTACTAGGCGGGGTGCCTGACCTGCCCGAGAGCTTCCCAGTCCGAATGCGG
CAGTTCGTGGGCTGCATGCGGAACCTGCAGGTGGACAGCCGGCACATAGACATGGCTGAC
TTCATTGCCAACAATGGCACCGTGCCTGGCTGCCCTGCCAAGAAGAACGTGTGTGACAGC
AACACTTGCCACAATGGGGGCACTTGCGTGAACCAGTGGGACGCGTTCAGCTGCGAGTGC
CCCCTGGGCTTTGGGGGCAAGAGCTGCGCCCAGGAAATGGCCAATCCACAGCACTTCCTG
GGCAGCAGCCTGGTGGCCTGGCATGGCCTCTCGCTGCCCATCTCCCAACCCTGGTACCTC
AGCCTCATGTTCCGCACGCGCCAGGCCGACGGTGTCCTGCTGCAGGCCATCACCAGGGGG
CGCAGCACCATCACCCTACAGCTACGAGAGGGCCACGTGATGCTGAGCGTGGAGGGCACA
GGGCTTCAGGCCTCCTCTCTCCGTCTGGAGCCAGGCCGGGCCAATGACGGTGACTGGCAC
CATGCACAGCTGGCACTGGGAGCCAGCGGGGGGCCCGGCCATGCCATTCTGTCCTTCGAT
TATGGGCAGCAGAGAGCAGAGGGCAACCTGGGCCCCCGGCTGCATGGTCTGCACCTGAGC
AACATAACAGTGGGCGGAATACCTGGGCCAGCCGGCGGTGTGGCCCGTGGCTTTCGGGGC
TGTTTGCAGGGTGTGCGGGTGAGCGATACGCCGGAGGGGGTTAACAGCCTGGATCCCAGC
CATGGGGAGAGCATCAACGTGGAGCAAGGCTGTAGCCTGCCTGACCCTTGTGACTCAAAC
CCGTGTCCTGCTAACAGCTATTGCAGCAACGACTGGGACAGCTATTCCTGCAGCTGTGAT
CCAGGTTACTATGGTGACAACTGTACTAATGTGTGTGACCTGAACCCGTGTGAGCACCAG
TCTGTGTGTACCCGCAAGCCCAGTGCCCCCCATGGCTATACCTGCGAGTGTCCCCCAAAT
TACCTTGGGCCATACTGTGAGACCAGGATTGACCAGCCTTGTCCCCGTGGCTGGTGGGGA
CATCCCACATGTGGCCCATGCAACTGTGATGTCAGCAAAGGCTTTGACCCAGACTGCAAC
AAGACAAGCGGCGAGTGCCACTGCAAGGAGAACCACTACCGGCCCCCAGGCAGCCCCACC
TGCCTCTTGTGTGACTGCTACCCCACAGGCTCCTTGTCCAGAGTCTGTGACCCTGAGGAT
GGCCAGTGTCCATGCAAGCCAGGTGTCATCGGGCGTCAGTGTGACCGCTGTGACAACCCT
TTTGCTGAGGTCACCACCAATGGCTGTGAAGTGAATTATGACAGCTGCCCACGAGCGATT
GAGGCTGGGATCTGGTGGCCCCGTACCCGCTTCGGGCTGCCTGCTGCTGCTCCCTGTCCC
AAAGGCTCCTTTGGGACTGCTGTGCGCCACTGTGATGAGCACAGGGGGTGGCTCCCCCCA
AACCTCTTCAACTGCACGTCCATCACCTTCTCAGAACTGAAGGGCTTCGCTGAGCGGCTA
CAGCGGAATGAGTCAGGCCTAGACTCAGGGCGCTCCCAGCAGCTAGCCCTGCTCCTGCGC
AACGCCACGCAGCACACAGCTGGCTACTTCGGCAGCGACGTCAAGGTGGCCTACCAGCTG
GCCACGCGGCTGCTGGCCCACGAGAGCACCCAGCGGGGCTTTGGGCTGTCTGCCACACAG
GACGTGCACTTCACTGAGAATCTGCTGCGGGTGGGCAGCGCCCTCCTGGACACAGCCAAC
AAGCGGCACTGGGAGCTGATCCAGCAGACAGAGGGTGGCACCGCCTGGCTGCTCCAGCAC
TATGAGGCCTACGCCAGTGCCCTGGCCCAGAACATGCGGCACACCTACCTAAGCCCCTTC
ACCATCGTCACGCCCAACATTGTCATCTCCGTAGTGCGCTTGGACAAAGGGAACTTTGCT
GGGGCCAAGCTGCCCCGCTACGAGGCCCTGCGTGGGGAGCAGCCCCCGGACCTTGAGACA
ACAGTCATTCTGCCTGAGTCTGTCTTCAGAGAGACGCCCCCCGTGGTCAGGCCCGCAGGC
CCCGGAGAGGCCCAGGAGCCAGAGGAGCTGGCACGGCGACAGCGACGGCACCCGGAGCTG
AGCCAGGGTGAGGCTGTGGCCAGCGTCATCATCTACCGCACCCTGGCCGGGCTACTGCCT
CATAACTATGACCCTGACAAGCGCAGCTTGAGAGTCCCCAAACGCCCGATCATCAACACA
CCCGTGGTGAGCATCAGCGTCCATGATGATGAGGAGCTTCTGCCCCGGGCCCTGGACAAA
CCCGTCACGGTGCAGTTCCGCCTGCTGGAGACAGAGGAGCGGACCAAGCCCATCTGTGTC
TTCTGGAACCATTCAATCCTGGTCAGTGGCACAGGTGGCTGGTCGGCCAGAGGCTGTGAA
GTCGTCTTCCGCAATGAGAGCCACGTCAGCTGCCAGTGCAACCACATGACGAGCTTCGCT
GTGCTCATGGACGTTTCTCGGCGGGAGAATGGGGAGATCCTGCCACTGAAGACACTGACA
TACGTGGCTCTAGGTGTCACCTTGGCTGCCCTTCTGCTCACCTTCTTCTTCCTCACTCTC
TTGCGTATCCTGCGCTCCAACCAACACGGCATCCGACGTAACCTGACAGCTGCCCTGGGC
CTGGCTCAGCTGGTCTTCCTCCTGGGAATCAACCAGGCTGACCTCCCTTTTGCCTGCACA
GTCATTGCCATCCTGCTGCACTTCCTGTACCTCTGCACCTTTTCCTGGGCTCTGCTGGAG
GCCTTGCACCTGTACCGGGCACTCACTGAGGTGCGCGATGTCAACACCGGCCCCATGCGC
TTCTACTACATGCTGGGCTGGGGCGTGCCTGCCTTCATCACAGGGCTAGCCGTGGGCCTG
GACCCCGAGGGCTACGGGAACCCTGACTTCTGCTGGCTCTCCATCTATGACACGCTCATC
TGGAGTTTTGCTGGCCCGGTGGCCTTTGCCGTCTCGATGAGTGTCTTCCTGTACATCCTG
GCGGCCCGGGCCTCCTGTGCTGCCCAGCGGCAGGGCTTTGAGAAGAAAGGTCCTGTCTCG
GGCCTGCAGCCCTCCTTCGCCGTCCTCCTGCTGCTGAGCGCCACGTGGCTGCTGGCACTG
CTCTCTGTCAACAGCGACACCCTCCTCTTCCACTACCTCTTTGCTACCTGCAATTGCATC
CAGGGCCCCTTCATCTTCCTCTCCTATGTGGTGCTTAGCAAGGAGGTCCGGAAAGCACTC
AAGCTTGCCTGCAGCCGCAAGCCCAGCCCTGACCCTGCTCTGACCACCAAGTCCACCCTG
ACCTCGTCCTACAACTGCCCCAGCCCCTACGCAGATGGGCGGCTGTACCAGCCCTACGGA
GACTCGGCCGGCTCTCTGCACAGCACCAGTCGCTCGGGCAAGAGTCAGCCCAGCTACATC
CCCTTCTTGCTGAGGGAGGAGTCCGCACTGAACCCTGGCCAAGGGCCCCCTGGCCTGGGG
GATCCAGGCAGCCTGTTCCTGGAAGGTCAAGACCAGCAGCATGATCCTGACACGGACTCC
GACAGTGACCTGTCCTTAGAAGACGACCAGAGTGGCTCCTATGCCTCTACCCACTCATCA
GACAGTGAGGAGGAAGAAGAGGAGGAGGAAGAGGAGGCCGCCTTCCCTGGAGAGCAGGGC
TGGGATAGCCTGCTGGGGCCTGGAGCAGAGAGACTGCCCCTGCACAGTACTCCCAAGGAT
GGGGGCCCAGGGCCTGGCAAGGCCCCCTGGCCAGGAGACTTTGGGACCACAGCAAAAGAG
AGTAGTGGCAACGGGGCCCCTGAGGAGCGGCTGCGGGAGAATGGAGATGCCCTGTCTCGA
GAGGGGTCCCTAGGCCCCCTTCCAGGCTCTTCTGCCCAGCCTCACAAAGGCATCCTTAAG
AAGAAGTGTCTGCCCACCATCAGCGAGAAGAGCAGCCTCCTGCGGCTCCCCCTGGAGCAA
TGCACAGGGTCTTCCCGGGGCTCCTCCGCTAGTGAGGGCAGCCGGGGAGGCCCCCCTCCC
CGCCCACCGCCCCGGCAGAGCCTCCAGGAGCAGCTGAACGGGGTCATGCCCATCGCCATG
AGCATCAAGGCAGGCACGGTGGATGAGGACTCGTCAGGCTCCGAATTTCTCTTCTTTAAC
TTCCTGCATTTG
Protein sequence predicted based on nucleotide sequence
MRSPATGVPLPTPPPPLLLLLLLLLPPPLLGDQVGPCRSLGSRGRGSSGACAPMGWLCPS SASNLWLYTSRCRDAGTELTGHLVPHHDGLRVWCPESEAHIPLPPAPEGCPWSCRLLGIG GHLSPQGKLTLPEEHPCLKAPRLRCQSCKLAQAPGLRAGERSPEESLGGRRKRNVNTAPQ FQPPSYQATVPENQPAGTPVASLRAIDPDEGEAGRLEYTMDALFDSRSNQFFSLDPVTGA VTTAEELDRETKSTHVFRVTAQDHGMPRRSALATLTILVTDTNDHDPVFEQQEYKESLRE NLEVGYEVLTVRATDGDAPPNANILYRLLEGSGGSPSEVFEIDPRSGVIRTRGPVDREEV ESYQLTVEASDQGRDPGPRSTTAAVFLSVEDDNDNAPQFSEKRYVVQVREDVTPGAPVLR VTASDRDKGSNAVVHYSIMSGNARGQFYLDAQTGALDVVSPLDYETTKEYTLRVRAQDGG RPPLSNVSGLVTVQVLDINDNAPIFVSTPFQATVLESVPLGYLVLHVQAIDADAGDNARL EYRLAGVGHDFPFTINNGTGWISVAAELDREEVDFYSFGVEARDHGTPALTASASVSVTV LDVNDNNPTFTQPEYTVRLNEDAAVGTSVVTVSAVDRDAHSVITYQITSGNTRNRFSITS QSGGGLVSLALPLDYKLERQYVLAVTASDGTRQDTAQIVVNVTDANTHRPVFQSSHYTVN VNEDRPAGTTVVLISATDEDTGENARITYFMEDSIPQFRIDADTGAVTTQAELDYEDQVS YTLAITARDNGIPQKSDTTYLEILVNDVNDNAPQFLRDSYQGSVYEDVPPFTSVLQISAT DRDSGLNGRVFYTFQGGDDGDGDFIVESTSGIVRTLRRLDRENVAQYVLRAYAVDKGMPP ARTPMEVTVTVLDVNDNPPVFEQDEFDVFVEENSPIGLAVARVTATDPDEGTNAQIMYQI VEGNIPEVFQLDIFSGELTALVDLDYEDRPEYVLVIQATSAPLVSRATVHVRLLDRNDNP PVLGNFEILFNNYVTNRSSSFPGGAIGRVPAHDPDISDSLTYSFERGNELSLVLLNASTG ELKLSRALDNNRPLEAIMSVLVSDGVHSVTAQCALRVTIITDEMLTHSITLRLEDMSPER FLSPLLGLFIQAVAATLATPPDHVVVFNVQRDTDAPGGHILNVSLSVGQPPGPGGGPPFL PSEDLQERLYLNRSLLTAISAQRVLPFDDNICLREPCENYMRCVSVLRFDSSAPFIASSS VLFRPIHPVGGLRCRCPPGFTGDYCETEVDLCYSRPCGPHGRCRSREGGYTCLCRDGYTG EHCEVSARSGRCTPGVCKNGGTCVNLLVGGFKCDCPSGDFEKPYCQVTTRSFPAHSFITF RGLRQRFHFTLALSFATKERDGLLLYNGRFNEKHDFVALEVIQEQVQLTFSAGESTTTVS PFVPGGVSDGQWHTVQLKYYNKPLLGQTGLPQGPSEQKVAVVTVDGCDTGVALRFGSVLG NYSCAAQGTQGGSKKSLDLTGPLLLGGVPDLPESFPVRMRQFVGCMRNLQVDSRHIDMAD FIANNGTVPGCPAKKNVCDSNTCHNGGTCVNQWDAFSCECPLGFGGKSCAQEMANPQHFL GSSLVAWHGLSLPISQPWYLSLMFRTRQADGVLLQAITRGRSTITLQLREGHVMLSVEGT GLQASSLRLEPGRANDGDWHHAQLALGASGGPGHAILSFDYGQQRAEGNLGPRLHGLHLS NITVGGIPGPAGGVARGFRGCLQGVRVSDTPEGVNSLDPSHGESINVEQGCSLPDPCDSN PCPANSYCSNDWDSYSCSCDPGYYGDNCTNVCDLNPCEHQSVCTRKPSAPHGYTCECPPN YLGPYCETRIDQPCPRGWWGHPTCGPCNCDVSKGFDPDCNKTSGECHCKENHYRPPGSPT CLLCDCYPTGSLSRVCDPEDGQCPCKPGVIGRQCDRCDNPFAEVTTNGCEVNYDSCPRAI EAGIWWPRTRFGLPAAAPCPKGSFGTAVRHCDEHRGWLPPNLFNCTSITFSELKGFAERL QRNESGLDSGRSQQLALLLRNATQHTAGYFGSDVKVAYQLATRLLAHESTQRGFGLSATQ DVHFTENLLRVGSALLDTANKRHWELIQQTEGGTAWLLQHYEAYASALAQNMRHTYLSPF TIVTPNIVISVVRLDKGNFAGAKLPRYEALRGEQPPDLETTVILPESVFRETPPVVRPAG PGEAQEPEELARRQRRHPELSQGEAVASVIIYRTLAGLLPHNYDPDKRSLRVPKRPIINT PVVSISVHDDEELLPRALDKPVTVQFRLLETEERTKPICVFWNHSILVSGTGGWSARGCE VVFRNESHVSCQCNHMTSFAVLMDVSRRENGEILPLKTLTYVALGVTLAALLLTFFFLTL LRILRSNQHGIRRNLTAALGLAQLVFLLGINQADLPFACTVIAILLHFLYLCTFSWALLE ALHLYRALTEVRDVNTGPMRFYYMLGWGVPAFITGLAVGLDPEGYGNPDFCWLSIYDTLI WSFAGPVAFAVSMSVFLYILAARASCAAQRQGFEKKGPVSGLQPSFAVLLLLSATWLLAL LSVNSDTLLFHYLFATCNCIQGPFIFLSYVVLSKEVRKALKLACSRKPSPDPALTTKSTL TSSYNCPSPYADGRLYQPYGDSAGSLHSTSRSGKSQPSYIPFLLREESALNPGQGPPGLG DPGSLFLEGQDQQHDPDTDSDSDLSLEDDQSGSYASTHSSDSEEEEEEEEEEAAFPGEQG WDSLLGPGAERLPLHSTPKDGGPGPGKAPWPGDFGTTAKESSGNGAPEERLRENGDALSR EGSLGPLPGSSAQPHKGILKKKCLPTISEKSSLLRLPLEQCTGSSRGSSASEGSRGGPPP RPPPRQSLQEQLNGVMPIAMSIKAGTVDEDSSGSEFLFFNFLHL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
