Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00879138
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 4755nts
Open reading frame: 1 to 4755
ATGGAGAATACAGGTTGGATGGGCAAGGGACATAGGATGACCCCAGCCTGTCCCCTCTTA
CTGTCTGTGATTCTGTCCCTGCGCCTGGCCACCGCCTTCGACCCCGCCCCCAGTGCCTGC
TCTGCCCTGGCCTCGGGTGTGCTCTACGGGGCCTTCTCGCTGCAGGACCTCTTTCCTACC
ATCGCCTCGGGCTGCTCCTGGACCCTGGAGAACCCTGACCCCACCAAGTACTCCCTCTAC
CTGCGCTTCAACCGCCAGGAGCAGGTGTGCGCACACTTTGCCCCCCGCCTGCTGCCCCTG
GACCACTACCTGGTCAACTTTACCTGCCTGCGGCCTAGCCCCGAGGAGGCGGTGGCCCAG
GCGGAGTCAGAGGTGGGGCGGCCAGAAGAGGAGGAGGCAGAGGCGGCAGCGGGGTTGGAG
CTGTGCAGCGGCTCAGGCCCCTTTACCTTCCTGCACTTCGACAAGAACTTCGTGCAGCTG
TGCCTGTCGGCTGAGCCCTCCGAGGCCCCGCGCCTGCTGGCGCCCGCTGCCCTAGCCTTC
CGCTTTGTCGAGGTCTTGCTCATCAACAACAACAACTCTAGCCAATTCACCTGTGGTGTG
CTCTGCCGCTGGAGTGAGGAGTGTGGCCGCGCTGCCGGCAGGGCCTGCGGCTTTGCTCAG
CCAGGCTGCAGCTGCCCTGGAGAGGCGGGGGCCGGCTCCACCACCACCACATCTCCAGGC
CCTCCTGCTGCCCACACCCTGTCCAATGCCCTGGTGCCCGGGGGCCCAGCCCCACCTGCT
GAGGCCGATTTGCACTCGGGGAGCAGCAATGATCTGTTCACAACCGAGATGAGATATGGT
GAGGAGCCGGAAGAGGAACCGAAAGTGAAAACCCAGTGGCCGAGGTCTGCAGATGAGCCT
GGGCTATACATGGCGCAGACAGGCGACCCGGCGGCTGAGGAGTGGTCCCCGTGGAGCGTG
TGTTCCCTGACGTGTGGGCAGGGTCTGCAGGTGCGGACCCGCTCCTGTGTGTCCTCCCCC
TATGGGACCCTGTGCAGCGGGCCCCTGCGGGAGACCAGGCCCTGCAACAATTCAGCCACC
TGCCCAGTGCACGGCGTGTGGGAGGAGTGGGGGTCCTGGAGCCTGTGCTCCCGCAGCTGC
GGGCGGGGGTCCCGGAGCCGGATGCGGACCTGCGTGCCCCCCCAGCACGGCGGCAAGGCC
TGCGAGGGTCCTGAGCTGCAGACTAAGCTCTGCAGTATGGCTGCCTGCCCGGTGGAAGGC
CAGTGGTTAGAATGGGGTCCCTGGGGCCCATGCTCCACGTCCTGTGCCAATGGGACCCAA
CAGCGCAGCCGGAAGTGCAGCGTGGCGGGCCCAGCCTGGGCCACATGCACGGGTGCCCTC
ACTGACACCCGGGAGTGCAGCAACCTCGAGTGCCCGGCCACTGATAGCAAGTGGGGGCCA
TGGAATGCGTGGAGCCTGTGCTCTAAGACGTGTGACACAGGCTGGCAGCGCCGCTTCCGC
ATGTGCCAGGCCACGGGCACGCAGGGCTACCCCTGCGAGGGCACCGGAGAGGAGGTGAAG
CCTTGTAGTGAGAAGAGGTGTCCAGCCTTCCATGAGATGTGCAGGGATGAGTACGTGATG
CTGATGACGTGGAAGAAGGCAGCTGCTGGCGAGATCATCTACAACAAGTGCCCCCCGAAT
GCCTCAGGGTCTGCCAGCCGCCGCTGTCTCCTCAGTGCCCAAGGCGTGGCGTACTGGGGG
CTGCCCAGCTTTGCTCGCTGCATCTCCCATGAGTACCGCTACCTGTATCTGTCACTTAGG
GAGCACCTGGCCAAGGGGCAGCGCATGCTGGCAGGCGAGGGCATGTCGCAGGTGGTGCGC
AGCCTGCAGGAGCTACTGGCCCGGCGCACCTACTATAGTGGGGACCTGCTCTTCTCTGTG
GACATTCTGAGGAATGTCACTGACACCTTTAAGAGGGCCACCTACGTGCCCTCGGCTGAT
GATGTGCAGCGCTTCTTCCAGGTGGTGAGCTTCATGGTGGATGCGGAAAACAAGGAGAAG
TGGGACGATGCTCAGCAGGTGTCCCCTGGCTCTGTGCACCTGCTCCGTGTCGTGGAGGAC
TTCATTCACCTGGTGGGCGATGCTCTCAAGGCCTTCCAGAGCTCTCTGATTGTCACAGAT
AATCTAGTGATCAGCATTCAGCGAGAGCCCGTCTCAGCTGTGTCCAGTGACATCACGTTC
CCCATGCGGGGCCGCCGGGGCATGAAGGACTGGGTGCGGCACTCAGAGGACCGCCTCTTC
CTGCCCAAGGAGGTGCTCAGCCTCTCCTCCCCAGGGAAGCCAGCCACATCTGGGGCAGCA
GGCAGCCCTGGCAGGGGGAGGGGCCCAGGAACGGTGCCTCCTGGCCCAGGCCACTCCCAC
CAGCGCCTCCTCCCAGCAGACCCTGATGAGTCCTCCTACTTTGTGATCGGTGCTGTACTC
TACCGCACCCTTGGCCTCATCCTGCCGCCTCCCAGGCCCCCGCTGGCCGTCACATCCCGG
GTGATGACAGTGACTGTGCGCCCCCCTACCCAGCCTCCAGCTGAGCCCCTCATCACTGTG
GAGCTCTCCTACATCATCAATGGGACCACGGATCCCCATTGCGCCAGCTGGGACTACTCC
AGAGCAGATGCCAGCTCAGGAGACTGGGACACTGAAAATTGCCAGACCCTGGAGACCCAG
GCAGCTCACACCCGCTGCCAGTGCCAGCACCTGTCCACCTTTGCTGTACTAGCCCAGCCG
CCCAAGGACCTGACCCTGGAGCTGGCGGGCTCCCCCTCGGTCCCCCTGGTGATCGGCTGT
GCAGTGTCGTGCATGGCGCTGCTCACCCTGCTCGCCATCTATGCCGCCTTTTGGAGGTTC
ATAAAATCTGAACGCTCCATCATCTTGCTGAACTTCTGCCTGTCCATCTTGGCATCCAAC
ATCCTGATCCTCGTGGGCCAGTCCCGGGTGCTGAGCAAGGGCGTGTGCACCATGACGGCT
GCCTTCCTGCACTTCTTCTTTCTCTCCTCCTTTTGCTGGGTGCTTACCGAGGCCTGGCAG
TCCTACCTGGCTGTCATTGGGCGGATGCGCACCCGCCTCGTTCGCAAGCGCTTCCTCTGC
CTGGGCTGGGGTCTGCCTGCCCTGGTGGTGGCCGTGTCTGTTGGCTTTACCCGAACGAAA
GGATACGGTACATCCAGCTACTGCTGGCTCTCCCTGGAGGGCGGCCTGCTCTACGCCTTT
GTGGGCCCTGCAGCCGTCATTGTCCTGGTGAACATGCTCATCGGAATCATCGTCTTCAAC
AAGCTCATGGCACGTGATGGCATCTCCGACAAATCCAAGAAGCAGAGGGCCGGGTCGGAG
CGGTGCCCCTGGGCCAGCCTGCTCCTCCCCTGCTCAGCGTGTGGAGCGGTCCCCAGCCCC
CTGCTCAGCTCAGCCTCGGCCAGGAACGCCATGGCCTCACTCTGGAGCTCCTGCGTGGTG
CTGCCCCTGCTGGCGCTCACCTGGATGTCTGCCGTCCTGGCTATGACAGACCGCCGTTCC
GTCCTCTTCCAGGCCCTCTTTGCTGTCTTCAACTCCGCGCAGGGCTTTGTCATCACTGCT
GTGCACTGCTTCCTGCGCCGAGAGGTCCAGGATGTGGTGAAGTGCCAGATGGGGGTGTGC
CGGGCTGATGAGAGCGAAGACTCCCCTGACTCGTGTAAGAACGGGCAGCTGCAGATCCTG
TCAGACTTTGAAAAGGATGTGGATCTGGCTTGTCAAACAGTGCTGTTCAAGGAGGTCAAC
ACTTGCAACCCGTCCACCATCACGGGCACACTATCCCGCCTGTCCCTGGATGAGGATGAG
GAGCCCAAGTCCTGCCTCGTGGGCCCTGAGGGCAGCCTCAGCTTCTCACCACTGCCTGGG
AATATCCTGGTGCCCATGGCAGCCTCACCAGGGCTGGGGGAGCCTCCGCCCCCACAGGAG
GCCAACCCTGTTTACATGTGTGGGGAGGGTGGCCTGCGGCAGCTGGACCTCACATGGCTG
CGGCCCACTGAGCCAGGCTCTGAGGGAGACTACATGGTGCTGCCCCGGCGGACTTTGAGC
CTGCAGCCTGGCGGTGGGGGTGGAGGTGGTGAGGATGCCCCCAGGGCCCGGCCGGAGGGG
ACCCCCCGGCGAGCTGCCAAGACAGTGGCCCACACTGAAGGCTACCCCAGCTTCCTGTCC
GTGGACCACTCGGGCCTGGGGCTGGGCCCTGCCTATGGATCTCTCCAGAATCCCTATGGA
ATGACCTTCCAACCGCCACCGCCGACACCCAGCGCCCGCCAAGTGCCCGAGCCAGGGGAG
CGCAGCCGGACCATGCCTCGCACCGTGCCCGGCTCTACCATGAAGATGGGCTCCCTGGAG
CGAAAGAAATTACGGTATTCAGACCTGGACTTTGAGGTGATGCACACCCGGAAACGGCAT
TCAGAACTCTACCACGAGCTCAACCAGAAGTTCCACACTTTCGACCGCTACCGCAGCCAG
TCCACGGCCAAGAGGGAGAAGCGGTGGAGTGTGTCCTCGGGTGGGGCAGCCGAGCGGAGC
GTGTGCACCGATAAGCCCAGCCCTGGGGAGCGCCCCAGCTTGTCCCAACATCGGCGCCAT
CAGAGCTGGAGCACCTTCAAATCTATGACACTGGGCTCGCTGCCCCCCAAGCCCCGAGAA
CGGCTGACTCTGCACCGGGCAGCAGCCTGGGAGCCCACAGAACCACCGGATGGTGACTTC
CAGACAGAGGTGTTG
Protein sequence predicted based on nucleotide sequence
MENTGWMGKGHRMTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPT IASGCSWTLENPDPTKYSLYLRFNRQEQVCAHFAPRLLPLDHYLVNFTCLRPSPEEAVAQ AESEVGRPEEEEAEAAAGLELCSGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAF RFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGSTTTTSPG PPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEP GLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSAT CPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEG QWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDSKWGP WNAWSLCSKTCDTGWQRRFRMCQATGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVM LMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLR EHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSAD DVQRFFQVVSFMVDAENKEKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTD NLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATSGAA GSPGRGRGPGTVPPGPGHSHQRLLPADPDESSYFVIGAVLYRTLGLILPPPRPPLAVTSR VMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTENCQTLETQ AAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRF IKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQ SYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAF VGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSP LLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITA VHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVN TCNPSTITGTLSRLSLDEDEEPKSCLVGPEGSLSFSPLPGNILVPMAASPGLGEPPPPQE ANPVYMCGEGGLRQLDLTWLRPTEPGSEGDYMVLPRRTLSLQPGGGGGGGEDAPRARPEG TPRRAAKTVAHTEGYPSFLSVDHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGE RSRTMPRTVPGSTMKMGSLERKKLRYSDLDFEVMHTRKRHSELYHELNQKFHTFDRYRSQ STAKREKRWSVSSGGAAERSVCTDKPSPGERPSLSQHRRHQSWSTFKSMTLGSLPPKPRE RLTLHRAAAWEPTEPPDGDFQTEVL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
