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Detailed Plasmid Information

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Explanation of Terms


Gene: Gene Symbol:  None
Gene Name:  None
Sequence             
Original Clone ID: 17183
Keyword: None
Species: Streptococcus mutans UA159
Type: cDNA
Vector Name: pMH4               Format:  CLOSED
Source: Joint Center for Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: PSI
Joint Center for Structural Genomics
SmCD00089876

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 1080nts         Open reading frame : 37 to 1116

ATGGGATCTGATAAAATTCATCATCATCATCATCACATGAATATTTATGATCAACTTCAG
GCGGTTGAAGATCGTTATGAAGAATTAGGAGAATTGCTTTCAGATCCAGACGTCGTCAGT
GATACCAAGCGTTTTATGGAATTATCGCGAGAAGAAGCCAATAGCCGCGAAACAGTTGCT
GTCTATCGTGAGTACAAGCAAATCGTTCAAAATATTGCTGATGCGCAAGAAATGATTAAA
GATGCTAGTGGTGATCCTGAACTTGAGGAAATGGCCAAGGAAGAGTTGAAAAACTCCAAG
GTAGCAAAGGAAGAATACGAAGAAAAACTGAAATTTCTCCTTTTACCAAAAGATCCCAAC
GATGATAAGAATATTATTTTAGAAATTCGTGGTGCTGCAGGCGGTGACGAAGCCGCTCTT
TTTGCTGGTGATCTTTTGAATATGTACCAAAAATATGCTGAAAATCAAGGCTGGAAATTT
GAGGTGATGGAGGCTTCTACCAATGGTGTCGGCGGACTCAAGGAAGTCGTTGCTATGGTT
TCAGGACAGTCTGTTTATTCTAAACTCAAATATGAATCGGGTGCCCATCGAGTTCAACGT
GTTCCTGTAACAGAAAGTCAAGGTCGTGTCCATACCTCAACAGCAACAGTTCTCGTCATG
CCCGAAGTTGAAGAAGTCGAATATGAAATAGATCCAAAAGACTTGCGTGTGGATATCTAT
CATGCTTCAGGTGCTGGTGGGCAAAATGTCAATAAAGTAGCAACTGCGGTTCGTATTATC
CATTTGCCAACCAACATTAAAGTTGAAATGCAAGAAGAGCGCACCCAGCAGAAAAATCGT
GATAAGGCTATGAAAATTATCCGTGCGCGTGTAGCAGACCACTTTGCGCAAATTGCTCAG
GATGAACAAGATGCTGAACGAAAATCAACAGTTGGTACGGGAGACCGTTCAGAGCGTATT
CGTACCTATAATTTTCCGCAAAACCGTGTCACAGATCACCGTATTGGGCTGACACTTCAA
AAATTAGACAGTATTTTGTCAGGCAAGTTAGACGAAGTCATTGACGCTCTTATTCTTTAT
GATCAAACACAAAAATTAGAAGAGCTTAATAAATGACGCGACTTAATTAACTCTAGATAA
CTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 1116
Mutations:
None
Discrepancy:
g166a,V56I;g296a,R99K;
g463a,A155T;t744c,I248I;
t954c,V318V;t1017c,D339D;
                                

5' Linker Sequence:
ATGGGATCTGATAAAATTCATCATCATCATCATCAC

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: SolubleProtein: Protein_Soluble
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
Find experimental protocols in 358002
Recommended expression in: DL41
       DyNA Vector Map

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Vector Name: pMH4

pMH4 in advanced viewed

Synonyms: pMH-4
Sequencing Primer: Forward:  pBAD forward
Reverse:  alt_bad_rev
Description: Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enyzme (Pml I/Pac I) or PIPE cloning.
Comments: None
Size (bp): 5489
Parent Vector: None
Empty Vector: None
Properties: PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name: Joint Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1 bacterial origin of replication 3331 4013
inducible trxn activator protein gene araC araC cds 5463 4585
negative selection marker ccdB ccdB negative selection gene (may be gone in with-insert form) 1576 1785
promoter T7 T7 promoter 290 310
promoter araC araC promoter 153 124
selectable marker CmR chloramphenicol resistance gene (CmR) 484 1140
selectable marker AmpR ampicillin resistance gene 2574 3233
tag His N-terminal 6xHis tag 364 381
trxn regulatory element arabinose O1 operator arabinose O1 operator 161 182
trxn regulatory element arabinose O2 operator arabinose O2 operator 5 23
trxn termination sequence rrnB rrnB T2 transcription terminator 2238 2264

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Reagents

Gene

Target GenBank: NP_721477

 

Protein

PDB - Structural Annotation : 1ZBT
SMART domain : PCRF :
Structural Annotation Wiki : TOPSAN
UniProt : Q8DU64
Protein Structure Viewer

FirstGlance in Jmol
PDB ID

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Clone

Cloning Information : 281058
HIP Master Clone ID : 210544
Original Clone ID : 17183

Experimental

Structural Biology Knowledgebase : SBKB (1ZBT)
TargetTrack - Experimental data : 358002

Reagents

Labome : rf1-cDNA