Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name:  None
Original Clone ID:
3269h1TCt7b1
Keyword:
None
Species:
Vibrio cholerae
Type:
cDNA
Vector Name:
pSB3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
C-terminal 6-His, TOPO
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
VcCD00091860
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 603nts
Open reading frame: 12 to 614

ATATGTCCCTTGGTACTATGTCTACTGAAGCAACTTTACTTGAACGCTGTCAATCACAAT
GCGAGCTATGCGCGGCCTCTACTCCTCTTTCTCCTTTTGTGGTTGCTCCGCATGCCCTCG
TCACTGTCGATCACGCAGTGATGCTGTGTGACACCTGTAAAGGACAAATCGAAAACCCAG
AAACGGTGGATATGAACCATTGGCGTTGTCTGAATGACAGTATGTGGAGCCAAGTTCAAC
CTGTTCAGGTTCTCGCATGGCGCCAACTGAAACGTATTTCTGAAACGGAAGGCTGGGCTC
GTGATCTGCTGGATATGATGTATCTTGATGAAGAGACCGCTAAGTGGGCAGAAATTGGCA
TGGACGATGATGCGGAAAAACCACGCGATGTAAATGGTGTCGAGCTGAAAAAAGGTGACG
ATGTCACCGTCATCAAAGATCTACCAGTCAAAGGCTCAAGCATGGTCATCAAACAAGGTA
CTGCAGTACGCGGTATCGCGTTAACCGAAGATCCAAAGCACATTCAAGGTAAGGTGAACG
GCCAAACCATGTACATCATTGCTGAGTATTGCCGTAAAAAGGAAGGGGGATCCCACCACC
ACCACCACCACTGAGATCCGAATTCGAGCTCCG

Protein sequence predicted based on nucleotide sequence

GTMSTEATLLERCQSQCELCAASTPLSPFVVAPHALVTVDHAVMLCDTCKGQIENPETVD
MNHWRCLNDSMWSQVQPVQVLAWRQLKRISETEGWARDLLDMMYLDEETAKWAEIGMDDD
AEKPRDVNGVELKKGDDVTVIKDLPVKGSSMVIKQGTAVRGIALTEDPKHIQGKVNGQTM
YIIAEYCRKKEGGSHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 603
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGTCCCTT
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-3269h
Recommended expression in:
Not Applicable