IL4I1 (Homo sapiens) in pLenti6.3/V5-DEST

Explanation of Terms

Gene:
Gene Symbol:  IL4I1
Gene Name:  interleukin 4 induced 1
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLenti6.3/V5-DEST
Format: 
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00942069
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1767nts
Open reading frame: 453 to 2219

GTTGGAGAAGAGGTAAGCGGTTACTCACTCCATGGCTGCAGCAAGGAGAGGCGGCGGCGG
CCTCGGCTGAAGAAAGAAGGTGGGAGCGGAGAGCGCAGGCGTGAAACCCACCTTGTCCCA
TCCACATCAGGACATCCCAGCTGGAGTTCAACCTTCATCCCTTCTGTGGCAGTTAGGAGA
CTGAATCAAGGTCCAGAGAAGGTGGAGGAATCCTGATACTGAGCGGAAGAAAATACACAG
ATGTGCTTCCTTCCCAGTCCTGACAAATGGCCTTTCCTTAAGTTCCTCATTAATTCATAT
GAAGACAACACATTTGGTGACTAAATTTGGAATCAGAGGCTTTTAAAGCCTCCCAGCTGC
TCTGACCCCAATATCAGGAACTTGGCATCTCTGATCTAACAAGGGCACCACTAACAAGGA
CAAAGCCACCATCATTCACCTTGATTCCGCACATGCCCAACGATGACTTCTGTCCTGGGC
TAACCATAAAGGCCATGGGTGCTGAGAGAGCCCCCCAGAGGCAGCCATGCACCCTGCACC
TCCTCGTCCTCGTCCCCATCCTCCTCAGCCTGGTGGCCTCCCAGGACTGGAAGGCTGAAC
GCAGCCAAGACCCCTTCGAGAAATGCATGCAGGATCCTGACTATGAGCAGCTGCTCAAGG
TGGTGACCTGGGGGCTCAATCGGACCCTGAAGCCCCAGAGGGTGATTGTGGTTGGCGCTG
GTGTGGCCGGGCTGGTGGCCGCCAAGGTGCTCAGCGATGCTGGACACAAGGTCACCATCC
TGGAGGCAGATAACAGGATCGGGGGCCGCATCTTCACCTACCGGGACCAGAACATGGGCT
GGATTGGGGAGCTGGGAGCCATGCGCATGCCCAGCTCTCACAGGATCCTCCACAAGCTCT
GCCAGGGCCTGGGGCTCAACCTGACCAAGTTCACCCAGTACGACAAGAACACGTGGACGG
AGGTGCACGAAGTGAAGCTGCGCAACTATGTGGTGGAGAAGGTGCCCGAGAAGCTGGGCT
ACGCCTTGCGTCCCCAGGAAAAGGGCCACTCGCCCGAAGACATCTACCAGATGGCTCTCA
ACCAGGCCCTCAAAGACCTCAAGGCACTGGGCTGCAGAAAGGCGATGAAGAAGTTTGAAA
GGCACACGCTCTTGGAATATCTTCTCGGGGAGGGGAACCTGAGCCGGCCGGCCGTGCAGC
TTCTGGGAGACGTGATGTCCGAGGATGGCTTCTTCTATCTCAGCTTCGCCGAGGCCCTCC
GGGCCCACAGCTGCCTCAGCGACAGACTCCAGTACAGCCGCATCGTGGGTGGCTGGGACC
TGCTGCCGCGCGCGCTGCTGAGCTCGCTGTCCGGGCTTGTGCTGTTGAACGCGCCCGTGG
TGGCGATGACCCAGGGACCGCACGATGTGCACGTGCAGATCGAGACCTCTCCCCCGGCGC
GGAATCTGAAGGTGCTGAAGGCCGACGTGGTGCTGCTGACGGCGAGCGGACCGGCGGTGA
AGCGCATCACCTTCTCGCCGCCGCTGCCCCGCCACATGCAGGAGGCGCTGCGGAGGCTGC
ACTACGTGCCGGCCACCAAGGTGTTCCTAAGCTTCCGCAGGCCCTTCTGGCGCGAGGAGC
ACATTGAAGGCGGCCACTCAAACACCGATCGCCCGTCGCGCATGATTTTCTACCCGCCGC
CGCGCGAGGGCGCGCTGCTGCTGGCCTCGTACACGTGGTCGGACGCGGCGGCAGCGTTCG
CCGGCTTGAGCCGGGAAGAGGCGTTGCGCTTGGCGCTCGACGACGTGGCGGCATTGCACG
GGCCTGTCGTGCGCCAGCTCTGGGACGGCACCGGCGTCGTCAAGCGTTGGGCGGAGGACC
AGCACAGCCAGGGTGGCTTTGTGGTACAGCCGCCGGCGCTCTGGCAAACCGAAAAGGATG
ACTGGACGGTCCCTTATGGCCGCATCTACTTTGCCGGCGAGCACACCGCCTACCCGCACG
GCTGGGTGGAGACGGCGGTCAAGTCGGCGCTGCGCGCCGCCATCAAGATCAACAGCCGGA
AGGGGCCTGCATCGGACACGGCCAGCCCCGAGGGGCACGCATCTGACATGGAGGGGCAGG
GGCATGTGCATGGGGTGGCCAGCAGCCCCTCGCATGACCTGGCAAAGGAAGAAGGCAGCC
ACCCTCCAGTCCAAGGCCAGTTATCTCTCCAAAACACGACCCACACGAGGACCTCGCATT
AAAGTATTTTCGGAAAAA

Protein sequence predicted based on nucleotide sequence

MPNDDFCPGLTIKAMGAERAPQRQPCTLHLLVLVPILLSLVASQDWKAERSQDPFEKCMQ
DPDYEQLLKVVTWGLNRTLKPQRVIVVGAGVAGLVAAKVLSDAGHKVTILEADNRIGGRI
FTYRDQNMGWIGELGAMRMPSSHRILHKLCQGLGLNLTKFTQYDKNTWTEVHEVKLRNYV
VEKVPEKLGYALRPQEKGHSPEDIYQMALNQALKDLKALGCRKAMKKFERHTLLEYLLGE
GNLSRPAVQLLGDVMSEDGFFYLSFAEALRAHSCLSDRLQYSRIVGGWDLLPRALLSSLS
GLVLLNAPVVAMTQGPHDVHVQIETSPPARNLKVLKADVVLLTASGPAVKRITFSPPLPR
HMQEALRRLHYVPATKVFLSFRRPFWREEHIEGGHSNTDRPSRMIFYPPPREGALLLASY
TWSDAAAAFAGLSREEALRLALDDVAALHGPVVRQLWDGTGVVKRWAEDQHSQGGFVVQP
PALWQTEKDDWTVPYGRIYFAGEHTAYPHGWVETAVKSALRAAIKINSRKGPASDTASPE
GHASDMEGQGHVHGVASSPSHDLAKEEGSHPPVQGQLSLQNTTHTRTSH

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Host Type:  bacterial    Marker:  chloramphenicol
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable