PIGQ (Homo sapiens) in pLenti6.3/V5-DEST

Explanation of Terms

Gene:
Gene Symbol:  PIGQ
Gene Name:  phosphatidylinositol glycan anchor biosynthesis, class Q
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLenti6.3/V5-DEST
Format: 
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00943182
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2283nts
Open reading frame: 98 to 2380

GGCACGAGGGTCTGCCCGGGCCCGCCCATCGGGGTCCCCAACCCCATCCGGACCCCGCCG
CCCGAGCGCGCGGCCCCGGAAGCACCCGCCTCCCGGCATGGTGCTCAAGGCCTTCTTCCC
CACGTGCTGCGTCTCGACGGACAGCGGGCTGCTGGTGGGACGGTGGGTGCCGGAGCAGAG
CAGCGCCGTGGTCCTGGCGGTCCTGCACTTTCCCTTCATCCCCATCCAGGTCAAGCAGCT
CCTGGCCCAGGTGCGGCAGGCCAGCCAGGTGGGCGTGGCCGTGCTGGGCACCTGGTGCCA
CTGCCGGCAGGAGCCCGAGGAGAGCCTGGGCCGCTTCCTGGAGAGCCTGGGTGCTGTCTT
CCCCCATGAGCCCTGGCTGCGGCTGTGCCGGGAGAGAGGCGGCACGTTCTGGAGCTGCGA
GGCCACCCACCGGCAAGCGCCCACTGCCCCCGGTGCCCCTGGTGAGGACCAGGTCATGCT
CATCTTCTATGACCAGCGCCAGGTGTTGCTGTCACAGCTACACCTGCCCACCGTCTTGCC
CGACCGCCAGGCTGGAGCCACCACTGCCAGCACGGGGGGCCTGGCTGCCGTCTTCGACAC
GGTAGCACGCAGTGAGGTGCTCTTCCGCAGTGACCGCTTTGATGAGGGCCCCGTGCGGCT
GAGCCACTGGCAGTCGGAGGGCGTGGAGGCCAGCATCCTCGCGGAGCTGGCCAGGCGAGC
CTCGGGACCCATTTGCCTGCTGTTGGCCAGCCTGCTGTCGCTGGTCTCAGCTGTCAGTGC
CTGCCGAGTGTTCAAGCTCTGGCCCCTGTCCTTCCTCGGGAGCAAACTCTCCACGTGCGA
ACAGCTCCGGCACCGGCTGGAGCACCTCACGCTAATCTTCAGTACACGGAAGGCGGAGAA
CCCTGCCCAGCTGATGAGGAAGGCCAACACGGTGGCCTCTGTGCTGCTGGACGTGGCCCT
GGGCCTCATGCTGCTGTCCTGGCTCCACGGGAGAAGCCGCATCGGGCATCTGGCCGACGC
CCTCGTTCCTGTGGCTGACCACGTGGCCGAGGAGCTCCAGCATCTGCTGCAGTGGCTGAT
GGGTGCTCCCGCCGGGCTCAAGATGAACCGTGCACTGGACCAGGTGCTGGGCCGCTTCTT
CCTCTACCACATCCACCTGTGGATCAGCTACATCCACCTCATGTCCCCCTTCGTGGAGCA
CATCCTTTGGCACGTGGGCCTCTCGGCCTGCCTGGGCCTGACGGTGGCCCTGTCCCTCCT
CTCGGACATTATCGCCCTCCTCACCTTCCACATCTACTGCTTTTACGTCTATGGAGCCAG
GCTGTACTGCCTGAAGATCCATGGCCTGTCCTCACTGTGGCGTCTGTTCCGGGGGAAGAA
GTGGAACGTTCTGCGCCAGCGCGTGGACTCCTGTTCCTATGACCTGGACCAGCTGTTCAT
CGGGACTCTGCTCTTCACCATCCTGCTCTTCCTCCTGCCTACCACAGCCCTGTACTACCT
GGTGTTCACCCTGCTCCGGCTCCTGGTGGTCGCCGTGCAGGGCCTGATCCATCTGCTCGT
GGACCTCATCAACTCCCTGCCGCTGTACTCACTGGGTCTTCGGCTCTGCCGGCCCTACAG
GCTGGCGGATAAACCCACTGCCCTACAGCCGCGTGGTGCACACCTACCGCCTCCCCAGCT
GTGGCTGCCACCCCAAGCACTCCTGGGGCGCCCTGTGCCGCAAGCTGTTCCTTGGGGAGC
TCATCTACCCCTGGAGGCAGAGAGGGGACAAGCAGGACTGAGGGAACTGCTGGCTCGCCT
GGCACCACCACACGGCCACAGCCAGCCATCTGCTCTGCCAGGGTGGCACCAGCTCAGCTG
GCGCATGTCCTGTGCTTTGTGGACGCTGCTGTGTGCTCCTGAACACGGCAGGCCCTGCTA
TCACACCTTGGGCTTGGAGGTCATTGGGAGTGAGCAGATGTGGGGGTGGCCAGCCAGGCT
GGCCGCACTCCATCACTGGCACTGCCTGCCTTGGGACCCGCTTCCCACCTGCTGCGGTCA
CCATGGTGGCGAGCACAGCAACCCCAGGTGTCCAGAGCACTGCCCCATGCCCACCCTGTG
TACCCAGGTCCAGAGGGTCCGTCCACCACAGCAGCCCCAGGTGGAGGGCTGGTCTCCCTG
GGGGCTCCCCAGTGGCTCTGCCCTGGCTGTGGGGGTGGAGGGACCTTGCCAGGATGAACC
CCCCAGTCCCAGGCACCCTCTAGCTCCCTCAGCCGAACAGCACCCTGCATCTGGGGGATT
GAAGCAGTCGCTGACCCCCGTCCCCAGCGGGCCCGGGCCCTCACTCCCTGAACCACACGG
GGTTTATTTGCGGATGTTCCCTGGAGAGGTCGCTTTGTGAAGAAACCATCAGCAGGCTGT
GAGCATCGCCAGGCTGCTGTGGGGGCGGGAGCAGCCTCAGTGTCAAGGGCCCGCCCACTG
ACCCAGCCGTACCTATTCGTCCACGGTGCCCCGTAGCAGCAGGTCCTGCGGCCAAATCTG
TCTCCCTTCATGGGCCTCCCAGGGAAGGAGGAAGCCCTGCTGTGCAGACACCTCTGTGGC
CCCCCAGGAGTGTGAGTGGCCTGGGGAGGGGGCCGTGGCACTGAGGCCGAAAGTGCCTGC
CAGACGGCACGGTCTGGGTGCGGGTGTTCCCTGTGAGCCCGAGTCCGCTTCAGGAGGGGA
GCCTGCAGGTGCCGGCTGGTGAGGGGATGACGCGCTGTGGGTGGGAGGAGGCAGCGCCCA
TCTCAGCAGCACCAGGACTGCCTGGGACTCCCTGGCAACCCAGCACCGGGGAAGCCGTCA
GCTGCTGTGACAATAAAACCTGCCCCGTGTCTGGAAAAAAAAAAAAAAAAAAA

Protein sequence predicted based on nucleotide sequence

MVLKAFFPTCCVSTDSGLLVGRWVPEQSSAVVLAVLHFPFIPIQVKQLLAQVRQASQVGV
AVLGTWCHCRQEPEESLGRFLESLGAVFPHEPWLRLCRERGGTFWSCEATHRQAPTAPGA
PGEDQVMLIFYDQRQVLLSQLHLPTVLPDRQAGATTASTGGLAAVFDTVARSEVLFRSDR
FDEGPVRLSHWQSEGVEASILAELARRASGPICLLLASLLSLVSAVSACRVFKLWPLSFL
GSKLSTCEQLRHRLEHLTLIFSTRKAENPAQLMRKANTVASVLLDVALGLMLLSWLHGRS
RIGHLADALVPVADHVAEELQHLLQWLMGAPAGLKMNRALDQVLGRFFLYHIHLWISYIH
LMSPFVEHILWHVGLSACLGLTVALSLLSDIIALLTFHIYCFYVYGARLYCLKIHGLSSL
WRLFRGKKWNVLRQRVDSCSYDLDQLFIGTLLFTILLFLLPTTALYYLVFTLLRLLVVAV
QGLIHLLVDLINSLPLYSLGLRLCRPYRLADKPTALQPRGAHLPPPQLWLPPQALLGRPV
PQAVPWGAHLPLEAERGQAGLRELLARLAPPHGHSQPSALPGWHQLSWRMSCALWTLLCA
PEHGRPCYHTLGLEVIGSEQMWGWPARLAALHHWHCLPWDPLPTCCGHHGGEHSNPRCPE
HCPMPTLCTQVQRVRPPQQPQVEGWSPWGLPSGSALAVGVEGPCQDEPPSPRHPLAPSAE
QHPASGGLKQSLTPVPSGPGPSLPEPHGVYLRMFPGEVAL*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Host Type:  bacterial    Marker:  chloramphenicol
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable